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Protein

Hexokinase-3

Gene

HK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi95 – 100ATPSequence analysis6
Nucleotide bindingi542 – 547ATPSequence analysis6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS08548-MONOMER.
ZFISH:HS08548-MONOMER.
ReactomeiR-HSA-6798695. Neutrophil degranulation.
R-HSA-70153. Glucose transport.
R-HSA-70171. Glycolysis.
SABIO-RKP52790.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-3 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type III
Short name:
HK III
Gene namesi
Name:HK3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:4925. HK3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi3101.
OpenTargetsiENSG00000160883.
PharmGKBiPA29303.

Chemistry databases

ChEMBLiCHEMBL2709.

Polymorphism and mutation databases

BioMutaiHK3.
DMDMi206729871.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001975901 – 923Hexokinase-3Add BLAST923

Proteomic databases

EPDiP52790.
MaxQBiP52790.
PaxDbiP52790.
PeptideAtlasiP52790.
PRIDEiP52790.

PTM databases

iPTMnetiP52790.
PhosphoSitePlusiP52790.
SwissPalmiP52790.

Expressioni

Gene expression databases

BgeeiENSG00000160883.
CleanExiHS_HK3.
ExpressionAtlasiP52790. baseline and differential.
GenevisibleiP52790. HS.

Organism-specific databases

HPAiHPA056743.
HPA059948.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi109348. 15 interactors.
IntActiP52790. 8 interactors.
STRINGi9606.ENSP00000292432.

Structurei

Secondary structure

1923
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi480 – 487Combined sources8
Helixi488 – 490Combined sources3
Helixi494 – 513Combined sources20
Beta strandi536 – 566Combined sources31
Helixi570 – 573Combined sources4
Helixi577 – 595Combined sources19
Beta strandi604 – 608Combined sources5
Helixi639 – 649Combined sources11
Beta strandi657 – 661Combined sources5
Helixi663 – 672Combined sources10
Beta strandi678 – 695Combined sources18
Helixi696 – 698Combined sources3
Beta strandi706 – 712Combined sources7
Helixi715 – 717Combined sources3
Turni718 – 727Combined sources10
Helixi730 – 737Combined sources8
Beta strandi739 – 741Combined sources3
Helixi748 – 750Combined sources3
Helixi753 – 769Combined sources17
Helixi774 – 776Combined sources3
Helixi780 – 783Combined sources4
Helixi790 – 796Combined sources7
Helixi803 – 811Combined sources9
Turni812 – 814Combined sources3
Helixi819 – 854Combined sources36
Beta strandi858 – 867Combined sources10
Helixi869 – 873Combined sources5
Helixi877 – 888Combined sources12
Beta strandi892 – 898Combined sources7
Helixi903 – 918Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HM8X-ray2.80A/B/C/D480-922[»]
ProteinModelPortaliP52790.
SMRiP52790.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52790.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 471Hexokinase 1PROSITE-ProRule annotationAdd BLAST445
Domaini477 – 912Hexokinase 2PROSITE-ProRule annotationAdd BLAST436

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 488RegulatoryAdd BLAST488
Regioni84 – 220Hexokinase small subdomain 1PROSITE-ProRule annotationAdd BLAST137
Regioni162 – 188Glucose-bindingSequence analysisAdd BLAST27
Regioni221 – 460Hexokinase large subdomain 1PROSITE-ProRule annotationAdd BLAST240
Regioni489 – 923CatalyticAdd BLAST435
Regioni531 – 661Hexokinase small subdomain 2PROSITE-ProRule annotationAdd BLAST131
Regioni603 – 629Glucose-bindingSequence analysisAdd BLAST27
Regioni662 – 901Hexokinase large subdomain 2PROSITE-ProRule annotationAdd BLAST240

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 2 hexokinase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1369. Eukaryota.
COG5026. LUCA.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiP52790.
KOiK00844.
OMAiSDCEGQD.
OrthoDBiEOG091G08MD.
PhylomeDBiP52790.
TreeFamiTF314238.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSIGSSGLR QGEETLSCSE EGLPGPSDSS ELVQECLQQF KVTRAQLQQI
60 70 80 90 100
QASLLGSMEQ ALRGQASPAP AVRMLPTYVG STPHGTEQGD FVVLELGATG
110 120 130 140 150
ASLRVLWVTL TGIEGHRVEP RSQEFVIPQE VMLGAGQQLF DFAAHCLSEF
160 170 180 190 200
LDAQPVNKQG LQLGFSFSFP CHQTGLDRST LISWTKGFRC SGVEGQDVVQ
210 220 230 240 250
LLRDAIRRQG AYNIDVVAVV NDTVGTMMGC EPGVRPCEVG LVVDTGTNAC
260 270 280 290 300
YMEEARHVAV LDEDRGRVCV SVEWGSFSDD GALGPVLTTF DHTLDHESLN
310 320 330 340 350
PGAQRFEKMI GGLYLGELVR LVLAHLARCG VLFGGCTSPA LLSQGSILLE
360 370 380 390 400
HVAEMEDPST GAARVHAILQ DLGLSPGASD VELVQHVCAA VCTRAAQLCA
410 420 430 440 450
AALAAVLSCL QHSREQQTLQ VAVATGGRVC ERHPRFCSVL QGTVMLLAPE
460 470 480 490 500
CDVSLIPSVD GGGRGVAMVT AVAARLAAHR RLLEETLAPF RLNHDQLAAV
510 520 530 540 550
QAQMRKAMAK GLRGEASSLR MLPTFVRATP DGSERGDFLA LDLGGTNFRV
560 570 580 590 600
LLVRVTTGVQ ITSEIYSIPE TVAQGSGQQL FDHIVDCIVD FQQKQGLSGQ
610 620 630 640 650
SLPLGFTFSF PCRQLGLDQG ILLNWTKGFK ASDCEGQDVV SLLREAITRR
660 670 680 690 700
QAVELNVVAI VNDTVGTMMS CGYEDPRCEI GLIVGTGTNA CYMEELRNVA
710 720 730 740 750
GVPGDSGRMC INMEWGAFGD DGSLAMLSTR FDASVDQASI NPGKQRFEKM
760 770 780 790 800
ISGMYLGEIV RHILLHLTSL GVLFRGQQIQ RLQTRDIFKT KFLSEIESDS
810 820 830 840 850
LALRQVRAIL EDLGLPLTSD DALMVLEVCQ AVSQRAAQLC GAGVAAVVEK
860 870 880 890 900
IRENRGLEEL AVSVGVDGTL YKLHPRFSSL VAATVRELAP RCVVTFLQSE
910 920
DGSGKGAALV TAVACRLAQL TRV
Length:923
Mass (Da):99,025
Last modified:September 23, 2008 - v2
Checksum:iDA75F2A9DBB895FF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti277F → L in AAC50732 (PubMed:8812439).Curated1
Sequence conflicti420Q → L in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti431E → Q in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti439V → I in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti510K → R in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti512 – 513LR → SE in AAC50422 (PubMed:8717435).Curated2
Sequence conflicti516A → S in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti519L → S in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti530 – 531PD → LT in AAC50422 (PubMed:8717435).Curated2
Sequence conflicti577 – 578GQ → AE in AAC50422 (PubMed:8717435).Curated2
Sequence conflicti604L → T in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti708R → H in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti718F → L in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti728S → R in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti750M → I in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti831A → V in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti836A → P in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti853E → G in AAC50732 (PubMed:8812439).Curated1
Sequence conflicti918A → T in AAC50422 (PubMed:8717435).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_034004281G → R.Corresponds to variant rs35610191dbSNPEnsembl.1
Natural variantiVAR_036186480R → W in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs376532514dbSNPEnsembl.1
Natural variantiVAR_036187499A → V in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs755546220dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51333 mRNA. Translation: AAC50732.1.
CH471195 Genomic DNA. Translation: EAW85048.1.
BC028129 mRNA. Translation: AAH28129.1.
U42303 mRNA. Translation: AAC50422.1.
CCDSiCCDS4407.1.
RefSeqiNP_002106.2. NM_002115.2.
UniGeneiHs.411695.

Genome annotation databases

EnsembliENST00000292432; ENSP00000292432; ENSG00000160883.
GeneIDi3101.
KEGGihsa:3101.
UCSCiuc003mfa.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Hexokinase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51333 mRNA. Translation: AAC50732.1.
CH471195 Genomic DNA. Translation: EAW85048.1.
BC028129 mRNA. Translation: AAH28129.1.
U42303 mRNA. Translation: AAC50422.1.
CCDSiCCDS4407.1.
RefSeqiNP_002106.2. NM_002115.2.
UniGeneiHs.411695.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HM8X-ray2.80A/B/C/D480-922[»]
ProteinModelPortaliP52790.
SMRiP52790.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109348. 15 interactors.
IntActiP52790. 8 interactors.
STRINGi9606.ENSP00000292432.

Chemistry databases

ChEMBLiCHEMBL2709.

PTM databases

iPTMnetiP52790.
PhosphoSitePlusiP52790.
SwissPalmiP52790.

Polymorphism and mutation databases

BioMutaiHK3.
DMDMi206729871.

Proteomic databases

EPDiP52790.
MaxQBiP52790.
PaxDbiP52790.
PeptideAtlasiP52790.
PRIDEiP52790.

Protocols and materials databases

DNASUi3101.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292432; ENSP00000292432; ENSG00000160883.
GeneIDi3101.
KEGGihsa:3101.
UCSCiuc003mfa.4. human.

Organism-specific databases

CTDi3101.
DisGeNETi3101.
GeneCardsiHK3.
HGNCiHGNC:4925. HK3.
HPAiHPA056743.
HPA059948.
MIMi142570. gene.
neXtProtiNX_P52790.
OpenTargetsiENSG00000160883.
PharmGKBiPA29303.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1369. Eukaryota.
COG5026. LUCA.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiP52790.
KOiK00844.
OMAiSDCEGQD.
OrthoDBiEOG091G08MD.
PhylomeDBiP52790.
TreeFamiTF314238.

Enzyme and pathway databases

UniPathwayiUPA00242.
BioCyciMetaCyc:HS08548-MONOMER.
ZFISH:HS08548-MONOMER.
ReactomeiR-HSA-6798695. Neutrophil degranulation.
R-HSA-70153. Glucose transport.
R-HSA-70171. Glycolysis.
SABIO-RKP52790.

Miscellaneous databases

EvolutionaryTraceiP52790.
GeneWikiiHK3.
GenomeRNAii3101.
PROiP52790.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160883.
CleanExiHS_HK3.
ExpressionAtlasiP52790. baseline and differential.
GenevisibleiP52790. HS.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHXK3_HUMAN
AccessioniPrimary (citable) accession number: P52790
Secondary accession number(s): Q8N1E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 23, 2008
Last modified: November 30, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.