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Protein

Hexokinase-3

Gene

HK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi95 – 1006ATPSequence Analysis
Nucleotide bindingi542 – 5476ATPSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS08548-MONOMER.
ReactomeiREACT_1383. Glycolysis.
REACT_212. Glucose transport.
SABIO-RKP52790.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-3 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type III
Short name:
HK III
Gene namesi
Name:HK3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:4925. HK3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29303.

Polymorphism and mutation databases

BioMutaiHK3.
DMDMi206729871.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 923923Hexokinase-3PRO_0000197590Add
BLAST

Proteomic databases

MaxQBiP52790.
PaxDbiP52790.
PRIDEiP52790.

PTM databases

PhosphoSiteiP52790.

Expressioni

Gene expression databases

BgeeiP52790.
CleanExiHS_HK3.
ExpressionAtlasiP52790. baseline and differential.
GenevestigatoriP52790.

Organism-specific databases

HPAiHPA056743.
HPA059948.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi109348. 10 interactions.
IntActiP52790. 3 interactions.
STRINGi9606.ENSP00000292432.

Structurei

Secondary structure

1
923
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi480 – 4878Combined sources
Helixi488 – 4903Combined sources
Helixi494 – 51320Combined sources
Beta strandi536 – 56631Combined sources
Helixi570 – 5734Combined sources
Helixi577 – 59519Combined sources
Beta strandi604 – 6085Combined sources
Helixi639 – 64911Combined sources
Beta strandi657 – 6615Combined sources
Helixi663 – 67210Combined sources
Beta strandi678 – 69518Combined sources
Helixi696 – 6983Combined sources
Beta strandi706 – 7127Combined sources
Helixi715 – 7173Combined sources
Turni718 – 72710Combined sources
Helixi730 – 7378Combined sources
Beta strandi739 – 7413Combined sources
Helixi748 – 7503Combined sources
Helixi753 – 76917Combined sources
Helixi774 – 7763Combined sources
Helixi780 – 7834Combined sources
Helixi790 – 7967Combined sources
Helixi803 – 8119Combined sources
Turni812 – 8143Combined sources
Helixi819 – 85436Combined sources
Beta strandi858 – 86710Combined sources
Helixi869 – 8735Combined sources
Helixi877 – 88812Combined sources
Beta strandi892 – 8987Combined sources
Helixi903 – 91816Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HM8X-ray2.80A/B/C/D480-922[»]
ProteinModelPortaliP52790.
SMRiP52790. Positions 28-919.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52790.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 471445Hexokinase 1PROSITE-ProRule annotationAdd
BLAST
Domaini477 – 912436Hexokinase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 488488RegulatoryAdd
BLAST
Regioni84 – 220137Hexokinase small subdomain 1PROSITE-ProRule annotationAdd
BLAST
Regioni162 – 18827Glucose-bindingSequence AnalysisAdd
BLAST
Regioni221 – 460240Hexokinase large subdomain 1PROSITE-ProRule annotationAdd
BLAST
Regioni489 – 923435CatalyticAdd
BLAST
Regioni531 – 661131Hexokinase small subdomain 2PROSITE-ProRule annotationAdd
BLAST
Regioni603 – 62927Glucose-bindingSequence AnalysisAdd
BLAST
Regioni662 – 901240Hexokinase large subdomain 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 2 hexokinase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiP52790.
KOiK00844.
OMAiHGTEQGD.
OrthoDBiEOG7S21X5.
PhylomeDBiP52790.
TreeFamiTF314238.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSIGSSGLR QGEETLSCSE EGLPGPSDSS ELVQECLQQF KVTRAQLQQI
60 70 80 90 100
QASLLGSMEQ ALRGQASPAP AVRMLPTYVG STPHGTEQGD FVVLELGATG
110 120 130 140 150
ASLRVLWVTL TGIEGHRVEP RSQEFVIPQE VMLGAGQQLF DFAAHCLSEF
160 170 180 190 200
LDAQPVNKQG LQLGFSFSFP CHQTGLDRST LISWTKGFRC SGVEGQDVVQ
210 220 230 240 250
LLRDAIRRQG AYNIDVVAVV NDTVGTMMGC EPGVRPCEVG LVVDTGTNAC
260 270 280 290 300
YMEEARHVAV LDEDRGRVCV SVEWGSFSDD GALGPVLTTF DHTLDHESLN
310 320 330 340 350
PGAQRFEKMI GGLYLGELVR LVLAHLARCG VLFGGCTSPA LLSQGSILLE
360 370 380 390 400
HVAEMEDPST GAARVHAILQ DLGLSPGASD VELVQHVCAA VCTRAAQLCA
410 420 430 440 450
AALAAVLSCL QHSREQQTLQ VAVATGGRVC ERHPRFCSVL QGTVMLLAPE
460 470 480 490 500
CDVSLIPSVD GGGRGVAMVT AVAARLAAHR RLLEETLAPF RLNHDQLAAV
510 520 530 540 550
QAQMRKAMAK GLRGEASSLR MLPTFVRATP DGSERGDFLA LDLGGTNFRV
560 570 580 590 600
LLVRVTTGVQ ITSEIYSIPE TVAQGSGQQL FDHIVDCIVD FQQKQGLSGQ
610 620 630 640 650
SLPLGFTFSF PCRQLGLDQG ILLNWTKGFK ASDCEGQDVV SLLREAITRR
660 670 680 690 700
QAVELNVVAI VNDTVGTMMS CGYEDPRCEI GLIVGTGTNA CYMEELRNVA
710 720 730 740 750
GVPGDSGRMC INMEWGAFGD DGSLAMLSTR FDASVDQASI NPGKQRFEKM
760 770 780 790 800
ISGMYLGEIV RHILLHLTSL GVLFRGQQIQ RLQTRDIFKT KFLSEIESDS
810 820 830 840 850
LALRQVRAIL EDLGLPLTSD DALMVLEVCQ AVSQRAAQLC GAGVAAVVEK
860 870 880 890 900
IRENRGLEEL AVSVGVDGTL YKLHPRFSSL VAATVRELAP RCVVTFLQSE
910 920
DGSGKGAALV TAVACRLAQL TRV
Length:923
Mass (Da):99,025
Last modified:September 23, 2008 - v2
Checksum:iDA75F2A9DBB895FF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti277 – 2771F → L in AAC50732 (PubMed:8812439).Curated
Sequence conflicti420 – 4201Q → L in AAC50422 (PubMed:8717435).Curated
Sequence conflicti431 – 4311E → Q in AAC50422 (PubMed:8717435).Curated
Sequence conflicti439 – 4391V → I in AAC50422 (PubMed:8717435).Curated
Sequence conflicti510 – 5101K → R in AAC50422 (PubMed:8717435).Curated
Sequence conflicti512 – 5132LR → SE in AAC50422 (PubMed:8717435).Curated
Sequence conflicti516 – 5161A → S in AAC50422 (PubMed:8717435).Curated
Sequence conflicti519 – 5191L → S in AAC50422 (PubMed:8717435).Curated
Sequence conflicti530 – 5312PD → LT in AAC50422 (PubMed:8717435).Curated
Sequence conflicti577 – 5782GQ → AE in AAC50422 (PubMed:8717435).Curated
Sequence conflicti604 – 6041L → T in AAC50422 (PubMed:8717435).Curated
Sequence conflicti708 – 7081R → H in AAC50422 (PubMed:8717435).Curated
Sequence conflicti718 – 7181F → L in AAC50422 (PubMed:8717435).Curated
Sequence conflicti728 – 7281S → R in AAC50422 (PubMed:8717435).Curated
Sequence conflicti750 – 7501M → I in AAC50422 (PubMed:8717435).Curated
Sequence conflicti831 – 8311A → V in AAC50422 (PubMed:8717435).Curated
Sequence conflicti836 – 8361A → P in AAC50422 (PubMed:8717435).Curated
Sequence conflicti853 – 8531E → G in AAC50732 (PubMed:8812439).Curated
Sequence conflicti918 – 9181A → T in AAC50422 (PubMed:8717435).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti281 – 2811G → R.
Corresponds to variant rs35610191 [ dbSNP | Ensembl ].
VAR_034004
Natural varianti480 – 4801R → W in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_036186
Natural varianti499 – 4991A → V in a breast cancer sample; somatic mutation. 1 Publication
VAR_036187

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51333 mRNA. Translation: AAC50732.1.
CH471195 Genomic DNA. Translation: EAW85048.1.
BC028129 mRNA. Translation: AAH28129.1.
U42303 mRNA. Translation: AAC50422.1.
CCDSiCCDS4407.1.
RefSeqiNP_002106.2. NM_002115.2.
UniGeneiHs.411695.

Genome annotation databases

EnsembliENST00000292432; ENSP00000292432; ENSG00000160883.
GeneIDi3101.
KEGGihsa:3101.
UCSCiuc003mez.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Hexokinase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51333 mRNA. Translation: AAC50732.1.
CH471195 Genomic DNA. Translation: EAW85048.1.
BC028129 mRNA. Translation: AAH28129.1.
U42303 mRNA. Translation: AAC50422.1.
CCDSiCCDS4407.1.
RefSeqiNP_002106.2. NM_002115.2.
UniGeneiHs.411695.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HM8X-ray2.80A/B/C/D480-922[»]
ProteinModelPortaliP52790.
SMRiP52790. Positions 28-919.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109348. 10 interactions.
IntActiP52790. 3 interactions.
STRINGi9606.ENSP00000292432.

Chemistry

ChEMBLiCHEMBL2709.

PTM databases

PhosphoSiteiP52790.

Polymorphism and mutation databases

BioMutaiHK3.
DMDMi206729871.

Proteomic databases

MaxQBiP52790.
PaxDbiP52790.
PRIDEiP52790.

Protocols and materials databases

DNASUi3101.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292432; ENSP00000292432; ENSG00000160883.
GeneIDi3101.
KEGGihsa:3101.
UCSCiuc003mez.3. human.

Organism-specific databases

CTDi3101.
GeneCardsiGC05M176240.
HGNCiHGNC:4925. HK3.
HPAiHPA056743.
HPA059948.
MIMi142570. gene.
neXtProtiNX_P52790.
PharmGKBiPA29303.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiP52790.
KOiK00844.
OMAiHGTEQGD.
OrthoDBiEOG7S21X5.
PhylomeDBiP52790.
TreeFamiTF314238.

Enzyme and pathway databases

UniPathwayiUPA00242.
BioCyciMetaCyc:HS08548-MONOMER.
ReactomeiREACT_1383. Glycolysis.
REACT_212. Glucose transport.
SABIO-RKP52790.

Miscellaneous databases

EvolutionaryTraceiP52790.
GeneWikiiHK3.
GenomeRNAii3101.
NextBioi12311.
PROiP52790.
SOURCEiSearch...

Gene expression databases

BgeeiP52790.
CleanExiHS_HK3.
ExpressionAtlasiP52790. baseline and differential.
GenevestigatoriP52790.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization."
    Furuta H., Nishi S., Le Beau M.M., Fernald A.A., Yano H., Bell G.I.
    Genomics 36:206-209(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Blood.
  4. "Purification and characterization of the carboxyl-domain of human hexokinase type III expressed as fusion protein."
    Palma F., Agostini D., Mason P., Dacha M., Piccoli G., Biagiarelli B., Fiorani M., Stocchi V.
    Mol. Cell. Biochem. 155:23-29(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 358-923.
    Tissue: Liver.
  5. Cited for: VARIANTS [LARGE SCALE ANALYSIS] TRP-480 AND VAL-499.

Entry informationi

Entry nameiHXK3_HUMAN
AccessioniPrimary (citable) accession number: P52790
Secondary accession number(s): Q8N1E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 23, 2008
Last modified: May 27, 2015
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.