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P52790

- HXK3_HUMAN

UniProt

P52790 - HXK3_HUMAN

Protein

Hexokinase-3

Gene

HK3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + D-hexose = ADP + D-hexose 6-phosphate.

    Enzyme regulationi

    Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi95 – 1006ATPSequence Analysis
    Nucleotide bindingi542 – 5476ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. fructokinase activity Source: RefGenome
    3. glucokinase activity Source: RefGenome
    4. hexokinase activity Source: MGI
    5. hormone binding Source: Ensembl
    6. mannokinase activity Source: RefGenome

    GO - Biological processi

    1. carbohydrate metabolic process Source: Reactome
    2. carbohydrate phosphorylation Source: GOC
    3. cellular glucose homeostasis Source: RefGenome
    4. glucose 6-phosphate metabolic process Source: GOC
    5. glucose transport Source: Reactome
    6. glycolytic process Source: UniProtKB-KW
    7. hexose metabolic process Source: UniProtKB-UniPathway
    8. hexose transport Source: Reactome
    9. small molecule metabolic process Source: Reactome
    10. transmembrane transport Source: Reactome

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS08548-MONOMER.
    ReactomeiREACT_212. Glucose transport.
    SABIO-RKP52790.
    UniPathwayiUPA00242.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hexokinase-3 (EC:2.7.1.1)
    Alternative name(s):
    Hexokinase type III
    Short name:
    HK III
    Gene namesi
    Name:HK3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:4925. HK3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. mitochondrion Source: Ensembl
    3. protein complex Source: Ensembl

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29303.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 923923Hexokinase-3PRO_0000197590Add
    BLAST

    Proteomic databases

    MaxQBiP52790.
    PaxDbiP52790.
    PRIDEiP52790.

    PTM databases

    PhosphoSiteiP52790.

    Expressioni

    Gene expression databases

    ArrayExpressiP52790.
    BgeeiP52790.
    CleanExiHS_HK3.
    GenevestigatoriP52790.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi109348. 3 interactions.
    IntActiP52790. 3 interactions.
    STRINGi9606.ENSP00000292432.

    Structurei

    Secondary structure

    1
    923
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi480 – 4878
    Helixi488 – 4903
    Helixi494 – 51320
    Beta strandi536 – 56631
    Helixi570 – 5734
    Helixi577 – 59519
    Beta strandi604 – 6085
    Helixi639 – 64911
    Beta strandi657 – 6615
    Helixi663 – 67210
    Beta strandi678 – 69518
    Helixi696 – 6983
    Beta strandi706 – 7127
    Helixi715 – 7173
    Turni718 – 72710
    Helixi730 – 7378
    Beta strandi739 – 7413
    Helixi748 – 7503
    Helixi753 – 76917
    Helixi774 – 7763
    Helixi780 – 7834
    Helixi790 – 7967
    Helixi803 – 8119
    Turni812 – 8143
    Helixi819 – 85436
    Beta strandi858 – 86710
    Helixi869 – 8735
    Helixi877 – 88812
    Beta strandi892 – 8987
    Helixi903 – 91816

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3HM8X-ray2.80A/B/C/D480-922[»]
    ProteinModelPortaliP52790.
    SMRiP52790. Positions 28-919.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP52790.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 231204Hexokinase type-1 1Add
    BLAST
    Domaini237 – 475239Hexokinase type-2 1Add
    BLAST
    Domaini480 – 675196Hexokinase type-1 2Add
    BLAST
    Domaini677 – 916240Hexokinase type-2 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 488488RegulatoryAdd
    BLAST
    Regioni162 – 18827Glucose-bindingSequence AnalysisAdd
    BLAST
    Regioni489 – 923435CatalyticAdd
    BLAST
    Regioni603 – 62927Glucose-bindingSequence AnalysisAdd
    BLAST

    Domaini

    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

    Sequence similaritiesi

    Belongs to the hexokinase family.Curated
    Contains 2 hexokinase type-1 domains.Curated
    Contains 2 hexokinase type-2 domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5026.
    HOGENOMiHOG000162671.
    HOVERGENiHBG005020.
    InParanoidiP52790.
    KOiK00844.
    OMAiHGTEQGD.
    OrthoDBiEOG7S21X5.
    PhylomeDBiP52790.
    TreeFamiTF314238.

    Family and domain databases

    InterProiIPR001312. Hexokinase.
    IPR022673. Hexokinase_C.
    IPR019807. Hexokinase_CS.
    IPR022672. Hexokinase_N.
    [Graphical view]
    PANTHERiPTHR19443. PTHR19443. 1 hit.
    PfamiPF00349. Hexokinase_1. 2 hits.
    PF03727. Hexokinase_2. 2 hits.
    [Graphical view]
    PRINTSiPR00475. HEXOKINASE.
    PROSITEiPS00378. HEXOKINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52790-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSIGSSGLR QGEETLSCSE EGLPGPSDSS ELVQECLQQF KVTRAQLQQI    50
    QASLLGSMEQ ALRGQASPAP AVRMLPTYVG STPHGTEQGD FVVLELGATG 100
    ASLRVLWVTL TGIEGHRVEP RSQEFVIPQE VMLGAGQQLF DFAAHCLSEF 150
    LDAQPVNKQG LQLGFSFSFP CHQTGLDRST LISWTKGFRC SGVEGQDVVQ 200
    LLRDAIRRQG AYNIDVVAVV NDTVGTMMGC EPGVRPCEVG LVVDTGTNAC 250
    YMEEARHVAV LDEDRGRVCV SVEWGSFSDD GALGPVLTTF DHTLDHESLN 300
    PGAQRFEKMI GGLYLGELVR LVLAHLARCG VLFGGCTSPA LLSQGSILLE 350
    HVAEMEDPST GAARVHAILQ DLGLSPGASD VELVQHVCAA VCTRAAQLCA 400
    AALAAVLSCL QHSREQQTLQ VAVATGGRVC ERHPRFCSVL QGTVMLLAPE 450
    CDVSLIPSVD GGGRGVAMVT AVAARLAAHR RLLEETLAPF RLNHDQLAAV 500
    QAQMRKAMAK GLRGEASSLR MLPTFVRATP DGSERGDFLA LDLGGTNFRV 550
    LLVRVTTGVQ ITSEIYSIPE TVAQGSGQQL FDHIVDCIVD FQQKQGLSGQ 600
    SLPLGFTFSF PCRQLGLDQG ILLNWTKGFK ASDCEGQDVV SLLREAITRR 650
    QAVELNVVAI VNDTVGTMMS CGYEDPRCEI GLIVGTGTNA CYMEELRNVA 700
    GVPGDSGRMC INMEWGAFGD DGSLAMLSTR FDASVDQASI NPGKQRFEKM 750
    ISGMYLGEIV RHILLHLTSL GVLFRGQQIQ RLQTRDIFKT KFLSEIESDS 800
    LALRQVRAIL EDLGLPLTSD DALMVLEVCQ AVSQRAAQLC GAGVAAVVEK 850
    IRENRGLEEL AVSVGVDGTL YKLHPRFSSL VAATVRELAP RCVVTFLQSE 900
    DGSGKGAALV TAVACRLAQL TRV 923
    Length:923
    Mass (Da):99,025
    Last modified:September 23, 2008 - v2
    Checksum:iDA75F2A9DBB895FF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti277 – 2771F → L in AAC50732. (PubMed:8812439)Curated
    Sequence conflicti420 – 4201Q → L in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti431 – 4311E → Q in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti439 – 4391V → I in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti510 – 5101K → R in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti512 – 5132LR → SE in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti516 – 5161A → S in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti519 – 5191L → S in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti530 – 5312PD → LT in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti577 – 5782GQ → AE in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti604 – 6041L → T in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti708 – 7081R → H in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti718 – 7181F → L in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti728 – 7281S → R in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti750 – 7501M → I in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti831 – 8311A → V in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti836 – 8361A → P in AAC50422. (PubMed:8717435)Curated
    Sequence conflicti853 – 8531E → G in AAC50732. (PubMed:8812439)Curated
    Sequence conflicti918 – 9181A → T in AAC50422. (PubMed:8717435)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti281 – 2811G → R.
    Corresponds to variant rs35610191 [ dbSNP | Ensembl ].
    VAR_034004
    Natural varianti480 – 4801R → W in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_036186
    Natural varianti499 – 4991A → V in a breast cancer sample; somatic mutation. 1 Publication
    VAR_036187

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51333 mRNA. Translation: AAC50732.1.
    CH471195 Genomic DNA. Translation: EAW85048.1.
    BC028129 mRNA. Translation: AAH28129.1.
    U42303 mRNA. Translation: AAC50422.1.
    CCDSiCCDS4407.1.
    RefSeqiNP_002106.2. NM_002115.2.
    UniGeneiHs.411695.

    Genome annotation databases

    EnsembliENST00000292432; ENSP00000292432; ENSG00000160883.
    GeneIDi3101.
    KEGGihsa:3101.
    UCSCiuc003mez.3. human.

    Polymorphism databases

    DMDMi206729871.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Wikipedia

    Hexokinase entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51333 mRNA. Translation: AAC50732.1 .
    CH471195 Genomic DNA. Translation: EAW85048.1 .
    BC028129 mRNA. Translation: AAH28129.1 .
    U42303 mRNA. Translation: AAC50422.1 .
    CCDSi CCDS4407.1.
    RefSeqi NP_002106.2. NM_002115.2.
    UniGenei Hs.411695.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3HM8 X-ray 2.80 A/B/C/D 480-922 [» ]
    ProteinModelPortali P52790.
    SMRi P52790. Positions 28-919.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109348. 3 interactions.
    IntActi P52790. 3 interactions.
    STRINGi 9606.ENSP00000292432.

    Chemistry

    BindingDBi P52790.
    ChEMBLi CHEMBL2709.

    PTM databases

    PhosphoSitei P52790.

    Polymorphism databases

    DMDMi 206729871.

    Proteomic databases

    MaxQBi P52790.
    PaxDbi P52790.
    PRIDEi P52790.

    Protocols and materials databases

    DNASUi 3101.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000292432 ; ENSP00000292432 ; ENSG00000160883 .
    GeneIDi 3101.
    KEGGi hsa:3101.
    UCSCi uc003mez.3. human.

    Organism-specific databases

    CTDi 3101.
    GeneCardsi GC05M176240.
    HGNCi HGNC:4925. HK3.
    MIMi 142570. gene.
    neXtProti NX_P52790.
    PharmGKBi PA29303.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5026.
    HOGENOMi HOG000162671.
    HOVERGENi HBG005020.
    InParanoidi P52790.
    KOi K00844.
    OMAi HGTEQGD.
    OrthoDBi EOG7S21X5.
    PhylomeDBi P52790.
    TreeFami TF314238.

    Enzyme and pathway databases

    UniPathwayi UPA00242 .
    BioCyci MetaCyc:HS08548-MONOMER.
    Reactomei REACT_212. Glucose transport.
    SABIO-RK P52790.

    Miscellaneous databases

    EvolutionaryTracei P52790.
    GeneWikii HK3.
    GenomeRNAii 3101.
    NextBioi 12311.
    PROi P52790.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P52790.
    Bgeei P52790.
    CleanExi HS_HK3.
    Genevestigatori P52790.

    Family and domain databases

    InterProi IPR001312. Hexokinase.
    IPR022673. Hexokinase_C.
    IPR019807. Hexokinase_CS.
    IPR022672. Hexokinase_N.
    [Graphical view ]
    PANTHERi PTHR19443. PTHR19443. 1 hit.
    Pfami PF00349. Hexokinase_1. 2 hits.
    PF03727. Hexokinase_2. 2 hits.
    [Graphical view ]
    PRINTSi PR00475. HEXOKINASE.
    PROSITEi PS00378. HEXOKINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization."
      Furuta H., Nishi S., Le Beau M.M., Fernald A.A., Yano H., Bell G.I.
      Genomics 36:206-209(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Blood.
    4. "Purification and characterization of the carboxyl-domain of human hexokinase type III expressed as fusion protein."
      Palma F., Agostini D., Mason P., Dacha M., Piccoli G., Biagiarelli B., Fiorani M., Stocchi V.
      Mol. Cell. Biochem. 155:23-29(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 358-923.
      Tissue: Liver.
    5. Cited for: VARIANTS [LARGE SCALE ANALYSIS] TRP-480 AND VAL-499.

    Entry informationi

    Entry nameiHXK3_HUMAN
    AccessioniPrimary (citable) accession number: P52790
    Secondary accession number(s): Q8N1E7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

    Keywords - Technical termi

    3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3