Hexokinase-3 - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P52790 (HXK3_HUMAN)

Last modified November 3, 2009. Version 84. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Hexokinase-3
    EC=2.7.1.1
Alternative name(s):
    Hexokinase type III
      Short name=HK III

Gene names

Name:

HK3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	923 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Enzyme regulation	Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.
Pathway	Carbohydrate metabolism; hexose metabolism.
Subunit structure	Monomer By similarity.
Domain	The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
Miscellaneous	In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).
Sequence similarities	Belongs to the hexokinase family.

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Ontologies

Keywords
Biological process	Glycolysis
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Non-traceable author statement. Source: UniProtKB
Cellular component	cytosol Inferred from Experiment. Source: Reactome membrane Non-traceable author statement. Source: UniProtKB
Molecular function	ATP binding Non-traceable author statement. Source: UniProtKB hexokinase activity Ref.4 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 923

923

Hexokinase-3

PRO_0000197590

Regions

Nucleotide binding

95 – 100

ATP Potential

Nucleotide binding

542 – 547

ATP Potential

Region

1 – 488

488

Regulatory

Region

162 – 188

Glucose-binding Potential

Region

489 – 923

435

Catalytic

Region

603 – 629

Glucose-binding Potential

Natural variations

Natural variant

281

G → R: dbSNP rs35610191.

VAR_034004

Natural variant

480

R → W in a colorectal cancer sample; somatic mutation. Ref.5

VAR_036186

Natural variant

499

A → V in a breast cancer sample; somatic mutation. Ref.5

VAR_036187

Experimental info

Sequence conflict

277

F → L in AAC50732. Ref.1

Sequence conflict

420

Q → L in AAC50422. Ref.4

Sequence conflict

431

E → Q in AAC50422. Ref.4

Sequence conflict

439

V → I in AAC50422. Ref.4

Sequence conflict

510

K → R in AAC50422. Ref.4

Sequence conflict

512 – 513

LR → SE in AAC50422. Ref.4

Sequence conflict

516

A → S in AAC50422. Ref.4

Sequence conflict

519

L → S in AAC50422. Ref.4

Sequence conflict

530 – 531

PD → LT in AAC50422. Ref.4

Sequence conflict

577 – 578

GQ → AE in AAC50422. Ref.4

Sequence conflict

604

L → T in AAC50422. Ref.4

Sequence conflict

708

R → H in AAC50422. Ref.4

Sequence conflict

718

F → L in AAC50422. Ref.4

Sequence conflict

728

S → R in AAC50422. Ref.4

Sequence conflict

750

M → I in AAC50422. Ref.4

Sequence conflict

831

A → V in AAC50422. Ref.4

Sequence conflict

836

A → P in AAC50422. Ref.4

Sequence conflict

853

E → G in AAC50732. Ref.1

Sequence conflict

918

A → T in AAC50422. Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

P52790-1 [UniParc].

Last modified September 23, 2008. Version 2.
Checksum: DA75F2A9DBB895FF
Show »

FASTA

923

99,025

        10         20         30         40         50         60 
MDSIGSSGLR QGEETLSCSE EGLPGPSDSS ELVQECLQQF KVTRAQLQQI QASLLGSMEQ 

        70         80         90        100        110        120 
ALRGQASPAP AVRMLPTYVG STPHGTEQGD FVVLELGATG ASLRVLWVTL TGIEGHRVEP 

       130        140        150        160        170        180 
RSQEFVIPQE VMLGAGQQLF DFAAHCLSEF LDAQPVNKQG LQLGFSFSFP CHQTGLDRST 

       190        200        210        220        230        240 
LISWTKGFRC SGVEGQDVVQ LLRDAIRRQG AYNIDVVAVV NDTVGTMMGC EPGVRPCEVG 

       250        260        270        280        290        300 
LVVDTGTNAC YMEEARHVAV LDEDRGRVCV SVEWGSFSDD GALGPVLTTF DHTLDHESLN 

       310        320        330        340        350        360 
PGAQRFEKMI GGLYLGELVR LVLAHLARCG VLFGGCTSPA LLSQGSILLE HVAEMEDPST 

       370        380        390        400        410        420 
GAARVHAILQ DLGLSPGASD VELVQHVCAA VCTRAAQLCA AALAAVLSCL QHSREQQTLQ 

       430        440        450        460        470        480 
VAVATGGRVC ERHPRFCSVL QGTVMLLAPE CDVSLIPSVD GGGRGVAMVT AVAARLAAHR 

       490        500        510        520        530        540 
RLLEETLAPF RLNHDQLAAV QAQMRKAMAK GLRGEASSLR MLPTFVRATP DGSERGDFLA 

       550        560        570        580        590        600 
LDLGGTNFRV LLVRVTTGVQ ITSEIYSIPE TVAQGSGQQL FDHIVDCIVD FQQKQGLSGQ 

       610        620        630        640        650        660 
SLPLGFTFSF PCRQLGLDQG ILLNWTKGFK ASDCEGQDVV SLLREAITRR QAVELNVVAI 

       670        680        690        700        710        720 
VNDTVGTMMS CGYEDPRCEI GLIVGTGTNA CYMEELRNVA GVPGDSGRMC INMEWGAFGD 

       730        740        750        760        770        780 
DGSLAMLSTR FDASVDQASI NPGKQRFEKM ISGMYLGEIV RHILLHLTSL GVLFRGQQIQ 

       790        800        810        820        830        840 
RLQTRDIFKT KFLSEIESDS LALRQVRAIL EDLGLPLTSD DALMVLEVCQ AVSQRAAQLC 

       850        860        870        880        890        900 
GAGVAAVVEK IRENRGLEEL AVSVGVDGTL YKLHPRFSSL VAATVRELAP RCVVTFLQSE 

       910        920 
DGSGKGAALV TAVACRLAQL TRV

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization." Furuta H., Nishi S., Le Beau M.M., Fernald A.A., Yano H., Bell G.I. Genomics 36:206-209(1996) [PubMed: 8812439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood.
[4]	"Purification and characterization of the carboxyl-domain of human hexokinase type III expressed as fusion protein." Palma F., Agostini D., Mason P., Dacha M., Piccoli G., Biagiarelli B., Fiorani M., Stocchi V. Mol. Cell. Biochem. 155:23-29(1996) [PubMed: 8717435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 358-923. Tissue: Liver.
[5]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] TRP-480 AND VAL-499.
+	Additional computationally mapped references.

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Web resources

Wikipedia

Hexokinase entry

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Cross-references

Sequence databases

U51333 mRNA. Translation: AAC50732.1.
CH471195 Genomic DNA. Translation: EAW85048.1.
BC028129 mRNA. Translation: AAH28129.1.
U42303 mRNA. Translation: AAC50422.1.

IPI

IPI00005118.

RefSeq

NP_002106.2.

UniGene

Hs.411695

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3HM8	X-ray	2.80	A/B/C/D	480-922	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P52790.

PTM databases

PhosphoSite

P52790.

Proteomic databases

PRIDE

P52790.

Genome annotation databases

Ensembl

ENST00000292432; ENSP00000292432; ENSG00000160883; Homo sapiens. [Genome view]

GeneID

3101.

KEGG

hsa:3101.

UCSC

uc003mfa.1. human.

Organism-specific databases

CTD

3101.

GeneCards

GC05M176240.

H-InvDB

HIX0005449.

HGNC

HGNC:4925. HK3.

MIM

142570. gene.

PharmGKB

PA29303.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P52790.

HOVERGEN

P52790.

OMA

TLISWTK.

Enzyme and pathway databases

BRENDA

2.7.1.1. 247.

Reactome

REACT_474. Metabolism of carbohydrates.

Gene expression databases

ArrayExpress

P52790.

Bgee

P52790.

CleanEx

HS_HK3.

Genevestigator

P52790.

GermOnline

ENSG00000160883. Homo sapiens.

Family and domain databases

InterPro

IPR001312. Hexokinase.
IPR019807. Hexokinase_CS.
[Graphical view]

PANTHER

PTHR19443. Hexokinase. 1 hit.

Pfam

PF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]

PRINTS

PR00475. HEXOKINASE.

ProDom

PD001109. Hexokinase. 2 hits.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00378. HEXOKINASES. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

12311.

SOURCE

Search...

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Entry information

Entry name

HXK3_HUMAN

Accession

Primary (citable) accession number: P52790
Secondary accession number(s): Q8N1E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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