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P52787

- IF_MOUSE

UniProt

P52787 - IF_MOUSE

Protein

Gastric intrinsic factor

Gene

Gif

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei171 – 1711CobalaminBy similarity
    Binding sitei222 – 2221CobalaminBy similarity
    Binding sitei270 – 2701CobalaminBy similarity

    GO - Molecular functioni

    1. cobalamin binding Source: RefGenome

    GO - Biological processi

    1. cobalamin metabolic process Source: RefGenome
    2. cobalamin transport Source: Ensembl
    3. cobalt ion transport Source: UniProtKB-KW

    Keywords - Biological processi

    Cobalt transport, Ion transport, Transport

    Keywords - Ligandi

    Cobalt

    Enzyme and pathway databases

    ReactomeiREACT_189086. Defective GIF causes intrinsic factor deficiency.
    REACT_189089. Defective AMN causes hereditary megaloblastic anemia 1.
    REACT_189090. Defective CUBN causes hereditary megaloblastic anemia 1.
    REACT_189118. Cobalamin (Cbl, vitamin B12) transport and metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gastric intrinsic factor
    Alternative name(s):
    Intrinsic factor
    Short name:
    IF
    Short name:
    INF
    Gene namesi
    Name:Gif
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1202394. Gif.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: Ensembl
    2. endosome Source: Ensembl
    3. extracellular space Source: Ensembl
    4. microvillus Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 417399Gastric intrinsic factorPRO_0000005559Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 246By similarity
    Disulfide bondi103 ↔ 288By similarity
    Disulfide bondi143 ↔ 182By similarity
    Glycosylationi311 – 3111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi413 – 4131N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP52787.
    PRIDEiP52787.

    PTM databases

    PhosphoSiteiP52787.

    Expressioni

    Tissue specificityi

    Gastric mucosa.

    Gene expression databases

    BgeeiP52787.
    CleanExiMM_GIF.
    GenevestigatoriP52787.

    Interactioni

    Subunit structurei

    Interacts with CUBN (via CUB domains).By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000025585.

    Structurei

    3D structure databases

    ProteinModelPortaliP52787.
    SMRiP52787. Positions 25-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni365 – 3706Cobalamin bindingBy similarity
    Regioni386 – 39510Cobalamin bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG254667.
    GeneTreeiENSGT00530000063370.
    HOGENOMiHOG000013214.
    HOVERGENiHBG006133.
    InParanoidiQ8C5C1.
    KOiK14615.
    OMAiNLAGAYN.
    OrthoDBiEOG7H1JKX.
    PhylomeDBiP52787.
    TreeFamiTF333092.

    Family and domain databases

    InterProiIPR002157. Cbl-bd_transpt_euk.
    [Graphical view]
    PANTHERiPTHR10559. PTHR10559. 1 hit.
    PfamiPF01122. Cobalamin_bind. 1 hit.
    [Graphical view]
    PROSITEiPS00468. COBALAMIN_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52787-1 [UniParc]FASTAAdd to Basket

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    MAWLTLYLLS VLWAVAGTST RAQSSCSVPP DQQPWVDGLQ ALMENSVTDS    50
    DFPNPSILIA MNLAGAYNVE AQKLLTYQLM ASDSADLTSG QLALTVMALT 100
    SSCRDPGSKV STLLKKMENW SPSSPGAESS AFYGPGLAIL ALCQKSSEAT 150
    LPIAVRFAKT LMMEPSPFNV DTGAVATLAL TCMYNKIPVG SQENYRDLFG 200
    QALKAIVEKI SLRIKADGII GDIYSTGLAM QALSVTPEQP TKKWDCEKTM 250
    HTILNEIKQG KFQNPMSIAQ ILPSLKGKTY LDVPQVTCGP DHEVPPTLTD 300
    YPTPVPTSVS NITVIYTINN QLRGVDLLFN VTIEVSVKSG SVLLAVLEEA 350
    QRKNSMFKFE TTMTSWGLIV SSINNIAENV NHKTYWEFLS GKTPLDEGVA 400
    YYIPFNHEHI TANFTQY 417
    Length:417
    Mass (Da):45,497
    Last modified:March 3, 2009 - v2
    Checksum:iBF38663EFC90F34A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti86 – 861D → N in AAA37881. (PubMed:8253786)Curated
    Sequence conflicti86 – 861D → N in AAA37882. (PubMed:8253786)Curated
    Sequence conflicti156 – 1561R → A in AAA37881. (PubMed:8253786)Curated
    Sequence conflicti156 – 1561R → A in AAA37882. (PubMed:8253786)Curated
    Sequence conflicti327 – 3271L → P in AAA37881. (PubMed:8253786)Curated
    Sequence conflicti327 – 3271L → P in AAA37882. (PubMed:8253786)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24192 Genomic DNA. Translation: AAA37881.1.
    L24191 mRNA. Translation: AAA37882.1.
    AK078933 mRNA. Translation: BAC37468.1.
    CH466534 Genomic DNA. Translation: EDL41439.1.
    BC118519 mRNA. Translation: AAI18520.1.
    CCDSiCCDS29609.1.
    PIRiA49684.
    RefSeqiNP_032144.2. NM_008118.3.
    UniGeneiMm.456.

    Genome annotation databases

    EnsembliENSMUST00000025585; ENSMUSP00000025585; ENSMUSG00000024682.
    GeneIDi14603.
    KEGGimmu:14603.
    UCSCiuc008gsw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24192 Genomic DNA. Translation: AAA37881.1 .
    L24191 mRNA. Translation: AAA37882.1 .
    AK078933 mRNA. Translation: BAC37468.1 .
    CH466534 Genomic DNA. Translation: EDL41439.1 .
    BC118519 mRNA. Translation: AAI18520.1 .
    CCDSi CCDS29609.1.
    PIRi A49684.
    RefSeqi NP_032144.2. NM_008118.3.
    UniGenei Mm.456.

    3D structure databases

    ProteinModelPortali P52787.
    SMRi P52787. Positions 25-417.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000025585.

    PTM databases

    PhosphoSitei P52787.

    Proteomic databases

    MaxQBi P52787.
    PRIDEi P52787.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025585 ; ENSMUSP00000025585 ; ENSMUSG00000024682 .
    GeneIDi 14603.
    KEGGi mmu:14603.
    UCSCi uc008gsw.1. mouse.

    Organism-specific databases

    CTDi 2694.
    MGIi MGI:1202394. Gif.

    Phylogenomic databases

    eggNOGi NOG254667.
    GeneTreei ENSGT00530000063370.
    HOGENOMi HOG000013214.
    HOVERGENi HBG006133.
    InParanoidi Q8C5C1.
    KOi K14615.
    OMAi NLAGAYN.
    OrthoDBi EOG7H1JKX.
    PhylomeDBi P52787.
    TreeFami TF333092.

    Enzyme and pathway databases

    Reactomei REACT_189086. Defective GIF causes intrinsic factor deficiency.
    REACT_189089. Defective AMN causes hereditary megaloblastic anemia 1.
    REACT_189090. Defective CUBN causes hereditary megaloblastic anemia 1.
    REACT_189118. Cobalamin (Cbl, vitamin B12) transport and metabolism.

    Miscellaneous databases

    NextBioi 286384.
    PROi P52787.
    SOURCEi Search...

    Gene expression databases

    Bgeei P52787.
    CleanExi MM_GIF.
    Genevestigatori P52787.

    Family and domain databases

    InterProi IPR002157. Cbl-bd_transpt_euk.
    [Graphical view ]
    PANTHERi PTHR10559. PTHR10559. 1 hit.
    Pfami PF01122. Cobalamin_bind. 1 hit.
    [Graphical view ]
    PROSITEi PS00468. COBALAMIN_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Use of transgenic mice to study regulation of gene expression in the parietal cell lineage of gastric units."
      Lorenz R.G., Gordon J.I.
      J. Biol. Chem. 268:26559-26570(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: BALB/c.
      Tissue: Stomach.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cecum.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiIF_MOUSE
    AccessioniPrimary (citable) accession number: P52787
    Secondary accession number(s): Q8C5C1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3