Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Gastric intrinsic factor

Gene

Gif

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei171 – 1711CobalaminBy similarity
Binding sitei222 – 2221CobalaminBy similarity
Binding sitei270 – 2701CobalaminBy similarity

GO - Molecular functioni

  1. cobalamin binding Source: MGI

GO - Biological processi

  1. cobalamin metabolic process Source: GO_Central
  2. cobalamin transport Source: MGI
  3. cobalt ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cobalt transport, Ion transport, Transport

Keywords - Ligandi

Cobalt

Enzyme and pathway databases

ReactomeiREACT_189086. Defective GIF causes intrinsic factor deficiency.
REACT_189089. Defective AMN causes hereditary megaloblastic anemia 1.
REACT_189090. Defective CUBN causes hereditary megaloblastic anemia 1.
REACT_189118. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Gastric intrinsic factor
Alternative name(s):
Intrinsic factor
Short name:
IF
Short name:
INF
Gene namesi
Name:Gif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1202394. Gif.

Subcellular locationi

GO - Cellular componenti

  1. apical plasma membrane Source: MGI
  2. endosome Source: MGI
  3. extracellular space Source: MGI
  4. microvillus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 417399Gastric intrinsic factorPRO_0000005559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 246By similarity
Disulfide bondi103 ↔ 288By similarity
Disulfide bondi143 ↔ 182By similarity
Glycosylationi311 – 3111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi413 – 4131N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP52787.
PRIDEiP52787.

PTM databases

PhosphoSiteiP52787.

Expressioni

Tissue specificityi

Gastric mucosa.

Gene expression databases

BgeeiP52787.
CleanExiMM_GIF.
GenevestigatoriP52787.

Interactioni

Subunit structurei

Interacts with CUBN (via CUB domains).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025585.

Structurei

3D structure databases

ProteinModelPortaliP52787.
SMRiP52787. Positions 25-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni365 – 3706Cobalamin bindingBy similarity
Regioni386 – 39510Cobalamin bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG254667.
GeneTreeiENSGT00530000063370.
HOGENOMiHOG000013214.
HOVERGENiHBG006133.
InParanoidiP52787.
KOiK14615.
OMAiNLAGAYN.
OrthoDBiEOG7H1JKX.
PhylomeDBiP52787.
TreeFamiTF333092.

Family and domain databases

InterProiIPR002157. Cbl-bd_transpt_euk.
[Graphical view]
PANTHERiPTHR10559. PTHR10559. 1 hit.
PfamiPF01122. Cobalamin_bind. 1 hit.
[Graphical view]
PROSITEiPS00468. COBALAMIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52787-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWLTLYLLS VLWAVAGTST RAQSSCSVPP DQQPWVDGLQ ALMENSVTDS
60 70 80 90 100
DFPNPSILIA MNLAGAYNVE AQKLLTYQLM ASDSADLTSG QLALTVMALT
110 120 130 140 150
SSCRDPGSKV STLLKKMENW SPSSPGAESS AFYGPGLAIL ALCQKSSEAT
160 170 180 190 200
LPIAVRFAKT LMMEPSPFNV DTGAVATLAL TCMYNKIPVG SQENYRDLFG
210 220 230 240 250
QALKAIVEKI SLRIKADGII GDIYSTGLAM QALSVTPEQP TKKWDCEKTM
260 270 280 290 300
HTILNEIKQG KFQNPMSIAQ ILPSLKGKTY LDVPQVTCGP DHEVPPTLTD
310 320 330 340 350
YPTPVPTSVS NITVIYTINN QLRGVDLLFN VTIEVSVKSG SVLLAVLEEA
360 370 380 390 400
QRKNSMFKFE TTMTSWGLIV SSINNIAENV NHKTYWEFLS GKTPLDEGVA
410
YYIPFNHEHI TANFTQY
Length:417
Mass (Da):45,497
Last modified:March 3, 2009 - v2
Checksum:iBF38663EFC90F34A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861D → N in AAA37881 (PubMed:8253786).Curated
Sequence conflicti86 – 861D → N in AAA37882 (PubMed:8253786).Curated
Sequence conflicti156 – 1561R → A in AAA37881 (PubMed:8253786).Curated
Sequence conflicti156 – 1561R → A in AAA37882 (PubMed:8253786).Curated
Sequence conflicti327 – 3271L → P in AAA37881 (PubMed:8253786).Curated
Sequence conflicti327 – 3271L → P in AAA37882 (PubMed:8253786).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24192 Genomic DNA. Translation: AAA37881.1.
L24191 mRNA. Translation: AAA37882.1.
AK078933 mRNA. Translation: BAC37468.1.
CH466534 Genomic DNA. Translation: EDL41439.1.
BC118519 mRNA. Translation: AAI18520.1.
CCDSiCCDS29609.1.
PIRiA49684.
RefSeqiNP_032144.2. NM_008118.3.
UniGeneiMm.456.

Genome annotation databases

EnsembliENSMUST00000025585; ENSMUSP00000025585; ENSMUSG00000024682.
GeneIDi14603.
KEGGimmu:14603.
UCSCiuc008gsw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24192 Genomic DNA. Translation: AAA37881.1.
L24191 mRNA. Translation: AAA37882.1.
AK078933 mRNA. Translation: BAC37468.1.
CH466534 Genomic DNA. Translation: EDL41439.1.
BC118519 mRNA. Translation: AAI18520.1.
CCDSiCCDS29609.1.
PIRiA49684.
RefSeqiNP_032144.2. NM_008118.3.
UniGeneiMm.456.

3D structure databases

ProteinModelPortaliP52787.
SMRiP52787. Positions 25-417.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025585.

PTM databases

PhosphoSiteiP52787.

Proteomic databases

MaxQBiP52787.
PRIDEiP52787.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025585; ENSMUSP00000025585; ENSMUSG00000024682.
GeneIDi14603.
KEGGimmu:14603.
UCSCiuc008gsw.1. mouse.

Organism-specific databases

CTDi2694.
MGIiMGI:1202394. Gif.

Phylogenomic databases

eggNOGiNOG254667.
GeneTreeiENSGT00530000063370.
HOGENOMiHOG000013214.
HOVERGENiHBG006133.
InParanoidiP52787.
KOiK14615.
OMAiNLAGAYN.
OrthoDBiEOG7H1JKX.
PhylomeDBiP52787.
TreeFamiTF333092.

Enzyme and pathway databases

ReactomeiREACT_189086. Defective GIF causes intrinsic factor deficiency.
REACT_189089. Defective AMN causes hereditary megaloblastic anemia 1.
REACT_189090. Defective CUBN causes hereditary megaloblastic anemia 1.
REACT_189118. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Miscellaneous databases

NextBioi286384.
PROiP52787.
SOURCEiSearch...

Gene expression databases

BgeeiP52787.
CleanExiMM_GIF.
GenevestigatoriP52787.

Family and domain databases

InterProiIPR002157. Cbl-bd_transpt_euk.
[Graphical view]
PANTHERiPTHR10559. PTHR10559. 1 hit.
PfamiPF01122. Cobalamin_bind. 1 hit.
[Graphical view]
PROSITEiPS00468. COBALAMIN_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Use of transgenic mice to study regulation of gene expression in the parietal cell lineage of gastric units."
    Lorenz R.G., Gordon J.I.
    J. Biol. Chem. 268:26559-26570(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: BALB/c.
    Tissue: Stomach.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cecum.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiIF_MOUSE
AccessioniPrimary (citable) accession number: P52787
Secondary accession number(s): Q8C5C1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 3, 2009
Last modified: February 4, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.