P52786 (CP2J1_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2J1
EC=1.14.14.1

Alternative name(s):
CYPIIJ1
Cytochrome P-450IB

Gene names

Name:

CYP2J1

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the N-demethylation of benzphetamine to formaldehyde.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Small intestine.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 501	500	Cytochrome P450 2J1		PRO_0000051768
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52786 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 90EEE628F0538197	FASTA	501	57,326
	10 20 30 40 50 60 MVAALSSLAA ALGAGLHPKT LLLGAVAFLF FAYFLKTRRP KNYPPGPWRL PFLGNLFTLD 70 80 90 100 110 120 MEKSHLQLQQ FVKKYGNLFC LDLAGKSIVI VTGLPLIKEV LVHMDQNFIN RPVPPIRERS 130 140 150 160 170 180 FKKNGLIMSS GQLWKEQRRF ALMTLRNFGL GKKSLEERIQ EEARHLTEAM EKEGGQPFDA 190 200 210 220 230 240 HFKINNAVSN IICSITFGER FEYHDGQFQE LLKLFDEVMY LEASMLCQLY NIFPWIMKFL 250 260 270 280 290 300 PGAHQTLFSN WKKLELFVSR MLENHKKDWN PAETRDFIDA YLKEMSKYPG SATSSFNEEN 310 320 330 340 350 360 LICSTLDLFL AGTETTSDMR WGLLFMALYP EIQEKVHAEI DSVIGQWQQP SMASRESLPY 370 380 390 400 410 420 TNAVIHEVQR MGNILPLNVP REVTVDTTLA GYHLPKGTVV LTNLTALHKD PEEWATPDTF 430 440 450 460 470 480 NPEHFLENGQ FKKKEAFIPF SIGKRACLGE QLAKSELFIF FTSLMQKFTF KPPSDEKLTL 490 500 NFRMGITLSP VKHRICAIPR A

References

[1]

"A novel species of cytochrome P-450 (P-450ib) specific for the small intestine of rabbits. cDNA cloning and its expression in COS cells." Kikuta Y., Sogawa K., Haniu M., Kinosaki M., Kusunose E., Nojima Y., Yamamoto S., Ichihara K., Kusunose M., Fujii-Kuriyama Y. J. Biol. Chem. 266:17821-17825(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D90405 mRNA. Translation: BAA14401.1.
PIR	A40938.
RefSeq	NP_001153760.1. NM_001160288.1.
UniGene	Ocu.2700.
3D structure databases
ProteinModelPortal	P52786.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100301544.
Organism-specific databases
CTD	100301544.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000036991.
HOVERGEN	HBG015789.
OrthoDB	EOG4QVCBZ.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008071. Cyt_P450_E_grp-I_CYP2J-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01688. EP450ICYP2J. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2J1_RABIT

Accession

Primary (citable) accession number: P52786

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program