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Protein

Guanylyl cyclase GC-E

Gene

Gucy2e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cGMP biosynthesis, Sensory transduction, Vision

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylyl cyclase GC-E (EC:4.6.1.2)
Alternative name(s):
Guanylate cyclase 2E
Gene namesi
Name:Gucy2e
Synonyms:Guc2e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:105123. Gucy2e.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini55 – 465411ExtracellularSequence analysisAdd
BLAST
Transmembranei466 – 49025HelicalSequence analysisAdd
BLAST
Topological domaini491 – 1108618CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5454Sequence analysisAdd
BLAST
Chaini55 – 11081054Guanylyl cyclase GC-EPRO_0000012383Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi108 ↔ 136By similarity
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence analysis
Disulfide bondi452 – 452InterchainBy similarity
Disulfide bondi460 – 460InterchainBy similarity

Post-translational modificationi

There are 9 conserved cysteine residues in sensory guanylate cyclases, 6 in the extracellular domain, which may be involved in intra- or interchain disulfide bonds.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP52785.
PaxDbiP52785.
PRIDEiP52785.

PTM databases

iPTMnetiP52785.
PhosphoSiteiP52785.

Expressioni

Gene expression databases

BgeeiP52785.
CleanExiMM_GUCY2E.
GenevisibleiP52785. MM.

Interactioni

Protein-protein interaction databases

BioGridi200127. 1 interaction.
STRINGi10090.ENSMUSP00000021259.

Structurei

3D structure databases

ProteinModelPortaliP52785.
SMRiP52785. Positions 563-841, 881-1059.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini491 – 811321Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini883 – 1013131Guanylate cyclasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000293307.
HOVERGENiHBG098487.
InParanoidiP52785.
KOiK12321.
OMAiAMLELTP.
OrthoDBiEOG7QVM1V.
TreeFamiTF106338.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011645. HNOB_dom_associated.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07701. HNOBA. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAWLLPAGG LPGAGFCVPA RQSPSSFSRV LRWPRPGLPG LLLLLLLPSP
60 70 80 90 100
SALSAVFKVG VLGPWACDPI FARARPDLAA RLAANRLNRD FALDGGPRFE
110 120 130 140 150
VALLPEPCLT PGSLGAVSSA LSRVSGLVGP VNPAACRPAE LLAQEAGVAL
160 170 180 190 200
VPWGCPGTRA AGTTAPAVTP AADALYVLLR AFRWARVALI TAPQDLWVEA
210 220 230 240 250
GRALSTALRA RGLPVALVTS METSDRSGAR EALGRIRDGP RVRVVIMVMH
260 270 280 290 300
SVLLGGEEQR YLLEAAEELA LTDGSLVFLP FDTLHYALSP GPEALAAFVN
310 320 330 340 350
SSQLRRAHDA VLTLTRRCPP GGSVQDSLRR AQEHQELPLD LNLKQVSPLF
360 370 380 390 400
GTIYDAVFLL AGGVKRARTA VGGGWVSGAS VARQVREAQV SGFCGVLGRT
410 420 430 440 450
EEPSFVLLDT DASGEQLFAT HLLDPVLGSL RSAGTPMHFP RGGPAPGPDP
460 470 480 490 500
SCWFDPDVIC NGGVEPGLVF VGFLLVIGMG LTGAFLAHYL RHRLLHMQMA
510 520 530 540 550
SGPNKIILTL EDVTFLHPPG GSSRKVVQGS RSSLATRSAS DIRSVPSQPQ
560 570 580 590 600
ESTNVGLYEG DWVWLKKFPG EHHMAIRPAT KTAFSKLREL RHENVALYLG
610 620 630 640 650
LFLAGTADSP ATPGEGILAV VSEHCARGSL HDLLAQREIK LDWMFKSSLL
660 670 680 690 700
LDLIKGMRYL HHRGVAHGRL KSRNCVVDGR FVLKVTDHGH GRLLEAQRVL
710 720 730 740 750
PEPPSAEDQL WTAPELLRDP SLERRGTLAG DVFSLAIIMQ EVVCRSTPYA
760 770 780 790 800
MLELTPEEVI QRVRSPPPLC RPLVSMDQAP MECIQLMTQC WAEHPELRPS
810 820 830 840 850
MDLTFDLFKS INKGRKTNII DSMLRMLEQY SSNLEDLIRE RTEELEQEKQ
860 870 880 890 900
KTDRLLTQML PPSVAEALKM GTSVEPEYFE EVTLYFSDIV GFTTISAMSE
910 920 930 940 950
PIEVVDLLND LYTLFDAIIG AHDVYKVETI GDAYMVASGL PQRNGQRHAA
960 970 980 990 1000
EIANMSLDIL SAVGSFRMRH MPEVPVRIRI GLHSGPCVAG VVGLTMPRYC
1010 1020 1030 1040 1050
LFGDTVNTAS RMESTGLPYR IHVNMSTVRI LRALDQGFQM ECRGRTELKG
1060 1070 1080 1090 1100
KGIEDTYWLV GRLGFNKPIP KPPDLQPGAS NHGISLQEIP PERRKKLEKA

RPGQFTGK
Length:1,108
Mass (Da):120,638
Last modified:July 27, 2011 - v2
Checksum:iF3F4FE69D38EB1C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151G → R in AAC42081 (PubMed:8838319).Curated
Sequence conflicti92 – 921A → G in AAC42081 (PubMed:8838319).Curated
Sequence conflicti135 – 1351A → G in AAC42081 (PubMed:8838319).Curated
Sequence conflicti1033 – 10331A → S in AAC42081 (PubMed:8838319).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41933 Genomic DNA. Translation: AAC42081.1.
AL645527 Genomic DNA. Translation: CAI35252.1.
CH466601 Genomic DNA. Translation: EDL10484.1.
CCDSiCCDS24887.1.
RefSeqiNP_032218.2. NM_008192.3.
XP_006532307.1. XM_006532244.2.
UniGeneiMm.291816.

Genome annotation databases

EnsembliENSMUST00000021259; ENSMUSP00000021259; ENSMUSG00000020890.
ENSMUST00000108664; ENSMUSP00000104304; ENSMUSG00000020890.
ENSMUST00000108665; ENSMUSP00000104305; ENSMUSG00000020890.
GeneIDi14919.
KEGGimmu:14919.
UCSCiuc007jpn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41933 Genomic DNA. Translation: AAC42081.1.
AL645527 Genomic DNA. Translation: CAI35252.1.
CH466601 Genomic DNA. Translation: EDL10484.1.
CCDSiCCDS24887.1.
RefSeqiNP_032218.2. NM_008192.3.
XP_006532307.1. XM_006532244.2.
UniGeneiMm.291816.

3D structure databases

ProteinModelPortaliP52785.
SMRiP52785. Positions 563-841, 881-1059.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200127. 1 interaction.
STRINGi10090.ENSMUSP00000021259.

PTM databases

iPTMnetiP52785.
PhosphoSiteiP52785.

Proteomic databases

MaxQBiP52785.
PaxDbiP52785.
PRIDEiP52785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021259; ENSMUSP00000021259; ENSMUSG00000020890.
ENSMUST00000108664; ENSMUSP00000104304; ENSMUSG00000020890.
ENSMUST00000108665; ENSMUSP00000104305; ENSMUSG00000020890.
GeneIDi14919.
KEGGimmu:14919.
UCSCiuc007jpn.2. mouse.

Organism-specific databases

CTDi14919.
MGIiMGI:105123. Gucy2e.

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000293307.
HOVERGENiHBG098487.
InParanoidiP52785.
KOiK12321.
OMAiAMLELTP.
OrthoDBiEOG7QVM1V.
TreeFamiTF106338.

Enzyme and pathway databases

ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

ChiTaRSiGucy2e. mouse.
PROiP52785.
SOURCEiSearch...

Gene expression databases

BgeeiP52785.
CleanExiMM_GUCY2E.
GenevisibleiP52785. MM.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011645. HNOB_dom_associated.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07701. HNOBA. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Chromosomal localization and genomic organization of genes encoding guanylyl cyclase receptors expressed in olfactory sensory neurons and retina."
    Yang R.B., Fulle H.J., Garbers D.L.
    Genomics 31:367-372(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiGUC2E_MOUSE
AccessioniPrimary (citable) accession number: P52785
Secondary accession number(s): B1ASX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.