6-phosphofructokinase, muscle type - Canis familiaris (Dog)

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P52784 (K6PF_CANFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
6-phosphofructokinase, muscle type
EC=2.7.1.11

Alternative name(s):
Phosphofructo-1-kinase isozyme A
Short name=PFK-A
Short name=Phosphofructokinase-M
Phosphofructokinase 1
Phosphohexokinase

Gene names

Name:	PFKM
Synonyms:	M-PFK

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis ›

Protein attributes

Sequence length	782 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP By similarity.
Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Cofactor	Magnesium.
Enzyme regulation	Allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Homotetramer By similarity.
Post-translational modification	GlcNAcylation decreases enzyme activity By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Glycoprotein Phosphoprotein
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glucose homeostasis Inferred from electronic annotation. Source: Compara glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway muscle cell homeostasis Inferred from electronic annotation. Source: Compara positive regulation of insulin secretion Inferred from electronic annotation. Source: Compara protein oligomerization Inferred from electronic annotation. Source: Compara
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro apical plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	6-phosphofructokinase activity Inferred from sequence or structural similarity. Source: UniProtKB ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose binding Inferred from electronic annotation. Source: Compara metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 782

781

6-phosphofructokinase, muscle type

PRO_0000112014

Regions

Repeat

2 – 404

403

Approximate

Repeat

405 – 782

378

Approximate

Nucleotide binding

35 – 39

ATP By similarity

Nucleotide binding

193 – 197

ATP By similarity

Nucleotide binding

210 – 226

ATP By similarity

Sites

Active site

166

Proton acceptor By similarity

Metal binding

224

Magnesium; via carbonyl oxygen By similarity

Binding site

201

Substrate By similarity

Binding site

292

Substrate By similarity

Binding site

298

Substrate By similarity

Binding site

301

Substrate By similarity

Amino acid modifications

Modified residue

N-acetylthreonine By similarity

Modified residue

669

Phosphoserine By similarity

Modified residue

777

Phosphoserine By similarity

Glycosylation

532

O-linked (GlcNAc...) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P52784 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 7C28514057CFE1C9
Show »

FASTA

782

85,561

        10         20         30         40         50         60 
MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF VHEGYQGLVD 

        70         80         90        100        110        120 
GGDHIREATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAHNLVKRGI TNLCVIGGDG 

       130        140        150        160        170        180 
SLTGADTFRS EWSDLLSDLQ KAGKITAEEA TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS 

       190        200        210        220        230        240 
ALHRIIEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE 

       250        260        270        280        290        300 
EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEEIK ELVVKRLGYD TRVTVLGHVQ 

       310        320        330        340        350        360 
RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME CVQVTKDVTK 

       370        380        390        400        410        420 
AMNDRKFDEA MKLRGRSFMN NWEVYKLLAH IRPPVSKTSA TMHTVAVMNV GAPAAGMNAA 

       430        440        450        460        470        480 
VRSTVRIGLI QGNRVLVVHD GFEGLAKGQI EEAGWSYVGG WTGQGGSKLG TKRTLPKKSF 

       490        500        510        520        530        540 
EQISANITKF NIQGLIIIGG FEAYTGGLEL MEGRKQFDEL CIPFVVIPAT VSNNVPGSDF 

       550        560        570        580        590        600 
SVGADTALNT ICTTCDRIKQ SAAGTKRRVF IIETMGGYCG YLATMAGLAA GADAAYIFEE 

       610        620        630        640        650        660 
PFTIRDLQAN VEHLVQKMKT TVKRGLVLRN EKCNENYTTD FIFNLYSEEG KGIFDSRKNV 

       670        680        690        700        710        720 
LGHMQQGGSP TPFDRNFATK MGAKAMNWMS GKIKESYRNG RIFANTPDSG CVLGMRKRAL 

       730        740        750        760        770        780 
VFQPVTELKD QTDFDHRIPK EQWWLKLRPI LKILAKYEID LDTTEHAHLE HISRKRSGET 


SI

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References

[1]	"A cDNA encoding canine muscle-type phosphofructokinase." Smith B.F., Henthorn P.S., Rajpurohit Y., Stedman H., Wolfe J.H., Patterson D.F., Giger U. Gene 168:275-276(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U25183 mRNA. Translation: AAC48615.1.
RefSeq	NP_001003199.1. NM_001003199.1.
UniGene	Cfa.3710.
3D structure databases
ProteinModelPortal	P52784.
ModBase	Search...
Protein-protein interaction databases
STRING	9615.ENSCAFP00000013277.
Proteomic databases
PaxDb	P52784.
PRIDE	P52784.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000046392; ENSCAFP00000037674; ENSCAFG00000009035.
GeneID	403849.
KEGG	cfa:403849.
Organism-specific databases
CTD	5213.
Phylogenomic databases
eggNOG	COG0205.
GeneTree	ENSGT00390000013209.
HOGENOM	HOG000200154.
HOVERGEN	HBG000976.
InParanoid	P52784.
KO	K00850.
OrthoDB	EOG4RJG0W.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20817343.

Entry information

Entry name

K6PF_CANFA

Accession

Primary (citable) accession number: P52784

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents