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P52784

- PFKAM_CANFA

UniProt

P52784 - PFKAM_CANFA

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Protein
ATP-dependent 6-phosphofructokinase, muscle type
Gene
PFKM, M-PFK
Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251ATP; via amide nitrogen By similarity
Metal bindingi119 – 1191Magnesium; catalytic By similarity
Active sitei166 – 1661Proton acceptor By similarity
Binding sitei201 – 2011Substrate; shared with dimeric partner By similarity
Binding sitei264 – 2641Substrate By similarity
Binding sitei292 – 2921Substrate; shared with dimeric partner By similarity
Binding sitei473 – 4731Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei568 – 5681Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei631 – 6311Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei657 – 6571Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei737 – 7371Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi88 – 892ATP By similarity
Nucleotide bindingi118 – 1214ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase, muscle type (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
PFK-M
Alternative name(s):
6-phosphofructokinase type A
Phosphofructo-1-kinase isozyme A
Short name:
PFK-A
Phosphohexokinase
Gene namesi
Name:PFKM
Synonyms:M-PFK
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Chromosome 27

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 782781ATP-dependent 6-phosphofructokinase, muscle typeUniRule annotation
PRO_0000112014Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine By similarity
Glycosylationi532 – 5321O-linked (GlcNAc) By similarity
Modified residuei669 – 6691Phosphoserine By similarity
Modified residuei777 – 7771Phosphoserine By similarity

Post-translational modificationi

GlcNAcylation decreases enzyme activity By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP52784.
PRIDEiP52784.

Interactioni

Subunit structurei

Homo- and heterotetramers By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000013277.

Structurei

3D structure databases

ProteinModelPortaliP52784.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 390389N-terminal catalytic PFK domain 1UniRule annotation
Add
BLAST
Regioni164 – 1663Substrate binding By similarity
Regioni208 – 2103Substrate binding By similarity
Regioni298 – 3014Substrate binding By similarity
Regioni391 – 40313Interdomain linkerUniRule annotation
Add
BLAST
Regioni404 – 782379C-terminal regulatory PFK domain 2UniRule annotation
Add
BLAST
Regioni530 – 5345Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni575 – 5773Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni663 – 6664Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
GeneTreeiENSGT00390000013209.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
InParanoidiP52784.
KOiK00850.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52784-1 [UniParc]FASTAAdd to Basket

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MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF    50
VHEGYQGLVD GGDHIREATW ESVSMMLQLG GTVIGSARCK DFREREGRLR 100
AAHNLVKRGI TNLCVIGGDG SLTGADTFRS EWSDLLSDLQ KAGKITAEEA 150
TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS ALHRIIEIVD AITTTAQSHQ 200
RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHLCRRLSET 250
RTRGSRLNII IVAEGAIDKN GKPITSEEIK ELVVKRLGYD TRVTVLGHVQ 300
RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME 350
CVQVTKDVTK AMNDRKFDEA MKLRGRSFMN NWEVYKLLAH IRPPVSKTSA 400
TMHTVAVMNV GAPAAGMNAA VRSTVRIGLI QGNRVLVVHD GFEGLAKGQI 450
EEAGWSYVGG WTGQGGSKLG TKRTLPKKSF EQISANITKF NIQGLIIIGG 500
FEAYTGGLEL MEGRKQFDEL CIPFVVIPAT VSNNVPGSDF SVGADTALNT 550
ICTTCDRIKQ SAAGTKRRVF IIETMGGYCG YLATMAGLAA GADAAYIFEE 600
PFTIRDLQAN VEHLVQKMKT TVKRGLVLRN EKCNENYTTD FIFNLYSEEG 650
KGIFDSRKNV LGHMQQGGSP TPFDRNFATK MGAKAMNWMS GKIKESYRNG 700
RIFANTPDSG CVLGMRKRAL VFQPVTELKD QTDFDHRIPK EQWWLKLRPI 750
LKILAKYEID LDTTEHAHLE HISRKRSGET SI 782
Length:782
Mass (Da):85,561
Last modified:January 23, 2007 - v2
Checksum:i7C28514057CFE1C9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25183 mRNA. Translation: AAC48615.1.
RefSeqiNP_001003199.1. NM_001003199.1.
UniGeneiCfa.3710.

Genome annotation databases

EnsembliENSCAFT00000046392; ENSCAFP00000037674; ENSCAFG00000009035.
GeneIDi403849.
KEGGicfa:403849.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25183 mRNA. Translation: AAC48615.1 .
RefSeqi NP_001003199.1. NM_001003199.1.
UniGenei Cfa.3710.

3D structure databases

ProteinModelPortali P52784.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9615.ENSCAFP00000013277.

Proteomic databases

PaxDbi P52784.
PRIDEi P52784.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSCAFT00000046392 ; ENSCAFP00000037674 ; ENSCAFG00000009035 .
GeneIDi 403849.
KEGGi cfa:403849.

Organism-specific databases

CTDi 5213.

Phylogenomic databases

eggNOGi COG0205.
GeneTreei ENSGT00390000013209.
HOGENOMi HOG000200154.
HOVERGENi HBG000976.
InParanoidi P52784.
KOi K00850.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Miscellaneous databases

NextBioi 20817343.

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.

Entry informationi

Entry nameiPFKAM_CANFA
AccessioniPrimary (citable) accession number: P52784
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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