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Protein

Glutamine synthetase cytosolic isozyme

Gene
N/A
Organism
Pinus sylvestris (Scots pine)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme (EC:6.3.1.2)
Alternative name(s):
GS1
Glutamate--ammonia ligase
OrganismiPinus sylvestris (Scots pine)
Taxonomic identifieri3349 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaePinusPinus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Glutamine synthetase cytosolic isozymePRO_0000153195Add
BLAST

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP52783.
SMRiP52783. Positions 5-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52783-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVLTDLLN LDLSDVTEKV IAEYIWIGGS GMDMRSKARS LSGPVSSVKE
60 70 80 90 100
LPKWNYDGSS TGQAQGHDSE VILYPQAIFR DPFRRGKHIL VICDAYSPNG
110 120 130 140 150
TAIPSNKRAA AAKIFNEKAV SDEETWYGLE QEYTLLQKDV KWPLGWPIGG
160 170 180 190 200
YPGPQGPYYC GVGADKAWGR DIVDAHYKAC LYSGINISGI NGEVMPGQWE
210 220 230 240 250
FQVGPSVGIS AADELWCARF IMERITEKAG VVLSFDPKPI EGDWNGAGCH
260 270 280 290 300
TNYSTKSMRK EGGFEVIKKA IEKLKLRHKE HISAYGEGNE RRLTGRHETA
310 320 330 340 350
DMNTFSWGVA NRGASVRVGR DTEKEGKGYF EDRRPASNMD PYIVTSMIAE

TTILWKP
Length:357
Mass (Da):39,571
Last modified:October 1, 1996 - v1
Checksum:i659B457032DB9F11
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti183 – 1831S → A in CAA52448 (PubMed:7506600).Curated
Sequence conflicti307 – 3071W → R in CAA52448 (PubMed:7506600).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69822 mRNA. Translation: CAA49476.1.
X74429 mRNA. Translation: CAA52448.1.
PIRiS36195.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69822 mRNA. Translation: CAA49476.1.
X74429 mRNA. Translation: CAA52448.1.
PIRiS36195.

3D structure databases

ProteinModelPortaliP52783.
SMRiP52783. Positions 5-356.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of a cDNA clone encoding glutamine synthetase from a gymnosperm, Pinus sylvestris."
    Canton F.R., Garcia-Gutierrez A., Gallardo F., de Vicente A., Canovas F.M.
    Plant Mol. Biol. 22:819-828(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Coaction of blue light and light absorbed by phytochrome in control of glutamine synthetase gene expression in Scots pine (Pinus sylvestris L.) seedlings."
    Elmlinger M.W., Bolle C., Batschauer A., Oelmueller R., Mohr H.
    Planta 192:189-194(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGLNA_PINSY
AccessioniPrimary (citable) accession number: P52783
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.