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P52783 (GLNA_PINSY) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase cytosolic isozyme

EC=6.3.1.2
Alternative name(s):
GS1
Glutamate--ammonia ligase
OrganismPinus sylvestris (Scots pine)
Taxonomic identifier3349 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaePinusPinus

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer By similarity.

Subcellular location

Cytoplasm.

Miscellaneous

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Glutamine synthetase cytosolic isozyme
PRO_0000153195

Experimental info

Sequence conflict1831S → A in CAA52448. Ref.2
Sequence conflict3071W → R in CAA52448. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P52783 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 659B457032DB9F11

FASTA35739,571
        10         20         30         40         50         60 
MSSVLTDLLN LDLSDVTEKV IAEYIWIGGS GMDMRSKARS LSGPVSSVKE LPKWNYDGSS 

        70         80         90        100        110        120 
TGQAQGHDSE VILYPQAIFR DPFRRGKHIL VICDAYSPNG TAIPSNKRAA AAKIFNEKAV 

       130        140        150        160        170        180 
SDEETWYGLE QEYTLLQKDV KWPLGWPIGG YPGPQGPYYC GVGADKAWGR DIVDAHYKAC 

       190        200        210        220        230        240 
LYSGINISGI NGEVMPGQWE FQVGPSVGIS AADELWCARF IMERITEKAG VVLSFDPKPI 

       250        260        270        280        290        300 
EGDWNGAGCH TNYSTKSMRK EGGFEVIKKA IEKLKLRHKE HISAYGEGNE RRLTGRHETA 

       310        320        330        340        350 
DMNTFSWGVA NRGASVRVGR DTEKEGKGYF EDRRPASNMD PYIVTSMIAE TTILWKP 

« Hide

References

[1]"Molecular characterization of a cDNA clone encoding glutamine synthetase from a gymnosperm, Pinus sylvestris."
Canton F.R., Garcia-Gutierrez A., Gallardo F., de Vicente A., Canovas F.M.
Plant Mol. Biol. 22:819-828(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Coaction of blue light and light absorbed by phytochrome in control of glutamine synthetase gene expression in Scots pine (Pinus sylvestris L.) seedlings."
Elmlinger M.W., Bolle C., Batschauer A., Oelmueller R., Mohr H.
Planta 192:189-194(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69822 mRNA. Translation: CAA49476.1.
X74429 mRNA. Translation: CAA52448.1.
PIRS36195.

3D structure databases

ProteinModelPortalP52783.
SMRP52783. Positions 5-356.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_PINSY
AccessionPrimary (citable) accession number: P52783
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families