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Protein

Glutamine--tRNA ligase

Gene
N/A
Organism
Lupinus luteus (European yellow lupin)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei508 – 5081ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligase (EC:6.1.1.18)
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name:
GlnRS
OrganismiLupinus luteus (European yellow lupin)
Taxonomic identifieri3873 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeGenisteaeLupinus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 794794Glutamine--tRNA ligasePRO_0000195864Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP52780.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi277 – 28711"HIGH" regionAdd
BLAST
Motifi505 – 5095"KMSKS" region

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PF04558. tRNA_synt_1c_R1. 1 hit.
PF04557. tRNA_synt_1c_R2. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAKDDTSSD KEKSLELFLK IGLDERTAKN TVANNKVTTN LTSVINDAGV
60 70 80 90 100
TDGCSRTVGN LLYTVATKYP ANALPHRPTL LQYIVNSKVK TTAQLDAALS
110 120 130 140 150
FLSATGSENL DLNKFEEACG VGVEVSTEDI KHAVDEVVEE NKATILELRY
160 170 180 190 200
RVNVGELLGH VRKRLPWADA KVVKQLVDAK LYEILGDRTA ADNEKPKKKK
210 220 230 240 250
EKPAKVEDKA APVATSEKPL EEDLNPYLIF PNPEDNFKVH TEVPFSDGNI
260 270 280 290 300
LRCCNTKALL EKHLKATGGK VLTRFPPEPN GYLHIGHAKA MFVDFGLAKD
310 320 330 340 350
RNGGCYLRFD DTNPEAEKKE YIDHIEEIVQ WMGWEPFKIT YTSNYFQELY
360 370 380 390 400
EFAVELIRRG HAYVDHQTAD EIKEYREKKL NSPWRDRPIS ESLKLFEDMR
410 420 430 440 450
RGFIEEGKAT LRMKQDMQSD NYNMYDLIAY RIKFTPHPHA GDKWCIYPSY
460 470 480 490 500
DYAHCIVDSI ENVTHSLCTL EFETRRASYY WLLHALGIYQ PYVWEYSRLN
510 520 530 540 550
VSNTVMSKRK LNRLVTEKWV DGWDDPRLMT LAGLRRRGMT PTAINAFVRG
560 570 580 590 600
MGITRSDGTL ISVERLEYHV REELNKTAPR AMVVLHPLKV VITNLEAKSA
610 620 630 640 650
IEVDAKKWPD AQADDASAFY KIPFSNVVYI ERSDFRMQDS KDYYGLAPGK
660 670 680 690 700
SVILRYAFPI KCTEVILADD NETILEIRAE YDPSKKTKPK GVLHWVSQPS
710 720 730 740 750
PGVDPLKVEV RLFERLFLSE NPAELDNWLG DLNPHSKVEI SNAYGVSLLK
760 770 780 790
DAKLGDRFQF ERLGYFAVDQ DSTPEKLVFN RTVTLKDSYG KGGK
Length:794
Mass (Da):90,483
Last modified:December 15, 1998 - v2
Checksum:i4C7D02C0795268E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91787 mRNA. Translation: CAA62901.1.
PIRiT09643.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91787 mRNA. Translation: CAA62901.1.
PIRiT09643.

3D structure databases

ProteinModelPortaliP52780.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PF04558. tRNA_synt_1c_R1. 1 hit.
PF04557. tRNA_synt_1c_R2. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of a cDNA clone encoding a plant gene of aminoacyl-tRNA synthetase."
    Siatecka M., Rozek M., Barciszewski J.
    Plant Gene Register PGR95-103
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Ventus.

Entry informationi

Entry nameiSYQ_LUPLU
AccessioniPrimary (citable) accession number: P52780
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 15, 1998
Last modified: October 1, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.