P52780 (SYQ_LUPLU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutamine--tRNA ligase EC=6.1.1.18 Alternative name(s): Glutaminyl-tRNA synthetase Short name=GlnRS
Organism	Lupinus luteus (European yellow lupin)
Taxonomic identifier	3873 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Genisteae › Lupinus

Protein attributes

Sequence length	794 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 794	794	Glutamine--tRNA ligase		PRO_0000195864
Regions
	Motif	277 – 287	11	"HIGH" region
	Motif	505 – 509	5	"KMSKS" region
Sites
	Binding site	508	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52780 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 4C7D02C0795268E1	FASTA	794	90,483
	10 20 30 40 50 60 MPAKDDTSSD KEKSLELFLK IGLDERTAKN TVANNKVTTN LTSVINDAGV TDGCSRTVGN 70 80 90 100 110 120 LLYTVATKYP ANALPHRPTL LQYIVNSKVK TTAQLDAALS FLSATGSENL DLNKFEEACG 130 140 150 160 170 180 VGVEVSTEDI KHAVDEVVEE NKATILELRY RVNVGELLGH VRKRLPWADA KVVKQLVDAK 190 200 210 220 230 240 LYEILGDRTA ADNEKPKKKK EKPAKVEDKA APVATSEKPL EEDLNPYLIF PNPEDNFKVH 250 260 270 280 290 300 TEVPFSDGNI LRCCNTKALL EKHLKATGGK VLTRFPPEPN GYLHIGHAKA MFVDFGLAKD 310 320 330 340 350 360 RNGGCYLRFD DTNPEAEKKE YIDHIEEIVQ WMGWEPFKIT YTSNYFQELY EFAVELIRRG 370 380 390 400 410 420 HAYVDHQTAD EIKEYREKKL NSPWRDRPIS ESLKLFEDMR RGFIEEGKAT LRMKQDMQSD 430 440 450 460 470 480 NYNMYDLIAY RIKFTPHPHA GDKWCIYPSY DYAHCIVDSI ENVTHSLCTL EFETRRASYY 490 500 510 520 530 540 WLLHALGIYQ PYVWEYSRLN VSNTVMSKRK LNRLVTEKWV DGWDDPRLMT LAGLRRRGMT 550 560 570 580 590 600 PTAINAFVRG MGITRSDGTL ISVERLEYHV REELNKTAPR AMVVLHPLKV VITNLEAKSA 610 620 630 640 650 660 IEVDAKKWPD AQADDASAFY KIPFSNVVYI ERSDFRMQDS KDYYGLAPGK SVILRYAFPI 670 680 690 700 710 720 KCTEVILADD NETILEIRAE YDPSKKTKPK GVLHWVSQPS PGVDPLKVEV RLFERLFLSE 730 740 750 760 770 780 NPAELDNWLG DLNPHSKVEI SNAYGVSLLK DAKLGDRFQF ERLGYFAVDQ DSTPEKLVFN 790 RTVTLKDSYG KGGK

References

[1]	"Isolation and characterization of a cDNA clone encoding a plant gene of aminoacyl-tRNA synthetase." Siatecka M., Rozek M., Barciszewski J. Plant Gene Register PGR95-103 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Ventus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X91787 mRNA. Translation: CAA62901.1.
PIR	T09643.
3D structure databases
ProteinModelPortal	P52780.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2. IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. PF04558. tRNA_synt_1c_R1. 1 hit. PF04557. tRNA_synt_1c_R2. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_LUPLU

Accession

Primary (citable) accession number: P52780

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 15, 1998
Last modified:	March 6, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program