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P52757 (CHIO_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-chimaerin
Alternative name(s):
Beta-chimerin
Rho GTPase-activating protein 3
Gene names
Name:CHN2
Synonyms:ARHGAP3, BCH
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase-activating protein for p21-rac. Insufficient expression of beta-2 chimaerin is expected to lead to higher Rac activity and could therefore play a role in the progression from low-grade to high-grade tumors.

Subcellular location

Membrane; Peripheral membrane protein Potential.

Tissue specificity

Highest levels in the brain and pancreas. Also expressed in the heart, placenta, and weakly in the kidney and liver. Expression is much reduced in the malignant gliomas, compared to normal brain or low-grade astrocytomas.

Sequence similarities

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 Rho-GAP domain.

Contains 1 SH2 domain.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSH2 domain
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionGTPase activation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processsmall GTPase mediated signal transduction

Traceable author statement. Source: Reactome

   Cellular componentcytosol

Traceable author statement. Source: Reactome

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionSH3/SH2 adaptor activity

Traceable author statement. Source: ProtInc

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]
Isoform Beta-2 (identifier: P52757-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta-1 (identifier: P52757-2)

The sequence of this isoform is not available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Beta-chimaerin
PRO_0000056697

Regions

Domain59 – 12769SH2
Domain277 – 468192Rho-GAP
Zinc finger214 – 26451Phorbol-ester/DAG-type

Natural variations

Natural variant2041H → R.
Corresponds to variant rs3750103 [ dbSNP | Ensembl ].
VAR_022118
Natural variant4381P → S.
Corresponds to variant rs34971642 [ dbSNP | Ensembl ].
VAR_049136

Experimental info

Sequence conflict1 – 66MAASSN → MRLL in AAA16836. Ref.1
Sequence conflict1 – 66MAASSN → MRLL in AAA19191. Ref.1

Secondary structure

...................................................................... 468
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Beta-2 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 63254958E0B5804C

FASTA46853,924
        10         20         30         40         50         60 
MAASSNSSLS GSSVSSDAEE YQPPIWKSYL YQLQQEAPRP KRIICPREVE NRPKYYGREF 

        70         80         90        100        110        120 
HGIISREQAD ELLGGVEGAY ILRESQRQPG CYTLALRFGN QTLNYRLFHD GKHFVGEKRF 

       130        140        150        160        170        180 
ESIHDLVTDG LITLYIETKA AEYISKMTTN PIYEHIGYAT LLREKVSRRL SRSKNEPRKT 

       190        200        210        220        230        240 
NVTHEEHTAV EKISSLVRRA ALTHNDNHFN YEKTHNFKVH TFRGPHWCEY CANFMWGLIA 

       250        260        270        280        290        300 
QGVRCSDCGL NVHKQCSKHV PNDCQPDLKR IKKVYCCDLT TLVKAHNTQR PMVVDICIRE 

       310        320        330        340        350        360 
IEARGLKSEG LYRVSGFTEH IEDVKMAFDR DGEKADISAN VYPDINIITG ALKLYFRDLP 

       370        380        390        400        410        420 
IPVITYDTYS KFIDAAKISN ADERLEAVHE VLMLLPPAHY ETLRYLMIHL KKVTMNEKDN 

       430        440        450        460 
FMNAENLGIV FGPTLMRPPE DSTLTTLHDM RYQKLIVQIL IENEDVLF 

« Hide

Isoform Beta-1 (Sequence not available).

References

« Hide 'large scale' references
[1]"Cerebellar beta 2-chimaerin, a GTPase-activating protein for p21 ras-related rac is specifically expressed in granule cells and has a unique N-terminal SH2 domain."
Leung T., How B.-E., Manser E., Lim L.
J. Biol. Chem. 269:12888-12892(1994) [PubMed: 8175705] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Cerebellum.
[2]"Identification and characterization of human beta 2-chimaerin: association with malignant transformation in astrocytoma."
Yuan S., Miller D.W., Barnett G.H., Hahn J.F., Williams B.R.G.
Cancer Res. 55:3456-3461(1995) [PubMed: 7614486] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fetal brain.
[3]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed: 12853948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L29126 mRNA. Translation: AAA19191.1.
U07223 mRNA. Translation: AAA16836.1.
U28926 mRNA. Translation: AAA86528.1.
AC004417 Genomic DNA. Translation: AAC06177.1.
AC007255 Genomic DNA. Translation: AAS07498.1.
BC112155 mRNA. Translation: AAI12156.1.
IPIIPI01012038.
PIRA53764.
RefSeqNP_001035025.1. NM_001039936.1.
NP_004058.1. NM_004067.2.
UniGeneHs.654611.
Hs.654753.
Hs.663145.
Hs.710429.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1XA6X-ray3.20A7-466[»]
ProteinModelPortalP52757.
SMRP52757. Positions 23-468.
ModBaseSearch...

Protein-protein interaction databases

IntActP52757. 2 interactions.
MINTMINT-1420866.
STRINGP52757.

PTM databases

PhosphoSiteP52757.

Polymorphism databases

DMDM2506455.

Proteomic databases

PRIDEP52757.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000222792; ENSP00000222792; ENSG00000106069.
GeneID1124.
KEGGhsa:1124.
UCSCuc003szz.1. human.

Organism-specific databases

CTD1124.
GeneCardsGC07P029186.
H-InvDBHIX0006558.
HGNCHGNC:1944. CHN2.
HPAHPA018989.
MIM602857. gene.
neXtProtNX_P52757.
GenAtlasSearch...

Phylogenomic databases

GeneTreeENSGT00600000084231.
HOVERGENHBG080489.
InParanoidP52757.
OMAXFGNQTL.
OrthoDBEOG4W9J3S.
PhylomeDBP52757.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP52757.
BgeeP52757.
CleanExHS_CHN2.
GenevestigatorP52757.
GermOnlineENSG00000106069. Homo sapiens.

Family and domain databases

InterProIPR020454. DAG/PE-bd.
IPR017356. N-chimaerin.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR000980. SH2.
[Graphical view]
Gene3DG3DSA:1.10.555.10. RhoGAP. 1 hit.
G3DSA:3.30.505.10. SH2. 1 hit.
PfamPF00130. C1_1. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PIRSFPIRSF038015. N-chimaerin. 1 hit.
PRINTSPR00008. DAGPEDOMAIN.
SMARTSM00109. C1. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMSSF48350. Rho_GAP. 1 hit.
PROSITEPS50238. RHOGAP. 1 hit.
PS50001. SH2. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio4668.
SOURCESearch...

Entry information

Entry nameCHIO_HUMAN
AccessionPrimary (citable) accession number: P52757
Secondary accession number(s): Q2M203, Q75MM2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: January 25, 2012
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families