P52757 (CHIO_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-chimaerin

Alternative name(s):
Beta-chimerin
Rho GTPase-activating protein 3

Gene names

Name:	CHN2
Synonyms:	ARHGAP3, BCH

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase-activating protein for p21-rac. Insufficient expression of beta-2 chimaerin is expected to lead to higher Rac activity and could therefore play a role in the progression from low-grade to high-grade tumors.
Subcellular location	Membrane; Peripheral membrane protein Potential.
Tissue specificity	Highest levels in the brain and pancreas. Also expressed in the heart, placenta, and weakly in the kidney and liver. Expression is much reduced in the malignant gliomas, compared to normal brain or low-grade astrocytomas.
Sequence similarities	Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Rho-GAP domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	SH2 domain Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	SH3/SH2 adaptor activity Traceable author statement. Source: ProtInc metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]

Isoform Beta-2 (identifier: P52757-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta-1 (identifier: P52757-2)

The sequence of this isoform is not available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 468

468

Beta-chimaerin

PRO_0000056697

Regions

Domain

59 – 127

69

SH2

Domain

277 – 468

192

Rho-GAP

Zinc finger

214 – 264

51

Phorbol-ester/DAG-type

Natural variations

Natural variant

204

1

H → R.
Corresponds to variant rs3750103 [ dbSNP | Ensembl ].

VAR_022118

Natural variant

438

1

P → S.
Corresponds to variant rs34971642 [ dbSNP | Ensembl ].

VAR_049136

Experimental info

Sequence conflict

1 – 6

6

MAASSN → MRLL in AAA16836. Ref.1

Sequence conflict

1 – 6

6

MAASSN → MRLL in AAA19191. Ref.1

Secondary structure

1

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468

Helix Strand Turn

Details...

Helix

29 – 36

8

Beta strand

58 – 63

6

Helix

66 – 73

8

Beta strand

79 – 84

6

Beta strand

86 – 88

3

Beta strand

92 – 98

7

Beta strand

101 – 104

4

Beta strand

108 – 114

7

Beta strand

116 – 118

3

Helix

125 – 138

14

Helix

141 – 146

6

Helix

153 – 155

3

Beta strand

156 – 161

6

Turn

229 – 231

3

Turn

237 – 240

4

Beta strand

246 – 248

3

Helix

255 – 257

3

Turn

266 – 269

4

Helix

279 – 286

8

Helix

292 – 303

12

Beta strand

306 – 308

3

Turn

309 – 313

5

Helix

318 – 328

11

Turn

331 – 334

4

Beta strand

339 – 342

4

Helix

346 – 358

13

Helix

369 – 373

5

Turn

374 – 377

4

Helix

381 – 392

12

Helix

397 – 405

9

Turn

406 – 410

5

Helix

411 – 414

4

Turn

415 – 419

5

Helix

424 – 431

8

Turn

432 – 434

3

Helix

445 – 448

4

Helix

450 – 462

13

Helix

464 – 467

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform Beta-2 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 63254958E0B5804C	FASTA	468	53,924
	10 20 30 40 50 60 MAASSNSSLS GSSVSSDAEE YQPPIWKSYL YQLQQEAPRP KRIICPREVE NRPKYYGREF 70 80 90 100 110 120 HGIISREQAD ELLGGVEGAY ILRESQRQPG CYTLALRFGN QTLNYRLFHD GKHFVGEKRF 130 140 150 160 170 180 ESIHDLVTDG LITLYIETKA AEYISKMTTN PIYEHIGYAT LLREKVSRRL SRSKNEPRKT 190 200 210 220 230 240 NVTHEEHTAV EKISSLVRRA ALTHNDNHFN YEKTHNFKVH TFRGPHWCEY CANFMWGLIA 250 260 270 280 290 300 QGVRCSDCGL NVHKQCSKHV PNDCQPDLKR IKKVYCCDLT TLVKAHNTQR PMVVDICIRE 310 320 330 340 350 360 IEARGLKSEG LYRVSGFTEH IEDVKMAFDR DGEKADISAN VYPDINIITG ALKLYFRDLP 370 380 390 400 410 420 IPVITYDTYS KFIDAAKISN ADERLEAVHE VLMLLPPAHY ETLRYLMIHL KKVTMNEKDN 430 440 450 460 FMNAENLGIV FGPTLMRPPE DSTLTTLHDM RYQKLIVQIL IENEDVLF
	Isoform Beta-1 (Sequence not available).		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cerebellar beta 2-chimaerin, a GTPase-activating protein for p21 ras-related rac is specifically expressed in granule cells and has a unique N-terminal SH2 domain." Leung T., How B.-E., Manser E., Lim L. J. Biol. Chem. 269:12888-12892(1994) [PubMed: 8175705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cerebellum.
[2]	"Identification and characterization of human beta 2-chimaerin: association with malignant transformation in astrocytoma." Yuan S., Miller D.W., Barnett G.H., Hahn J.F., Williams B.R.G. Cancer Res. 55:3456-3461(1995) [PubMed: 7614486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L29126 mRNA. Translation: AAA19191.1.
U07223 mRNA. Translation: AAA16836.1.
U28926 mRNA. Translation: AAA86528.1.
AC004417 Genomic DNA. Translation: AAC06177.1.
AC007255 Genomic DNA. Translation: AAS07498.1.
BC112155 mRNA. Translation: AAI12156.1.

IPI

IPI01012038.

PIR

A53764.

RefSeq

NP_001035025.1. NM_001039936.1.
NP_004058.1. NM_004067.2.

UniGene

Hs.654611.
Hs.654753.
Hs.663145.
Hs.710429.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XA6	X-ray	3.20	A	7-466	[»]

ProteinModelPortal

P52757.

SMR

P52757. Positions 23-468.

ModBase

Search...

Protein-protein interaction databases

IntAct

P52757. 2 interactions.

MINT

MINT-1420866.

STRING

P52757.

PTM databases

PhosphoSite

P52757.

Polymorphism databases

DMDM

2506455.

Proteomic databases

PRIDE

P52757.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000222792; ENSP00000222792; ENSG00000106069.

GeneID

1124.

KEGG

hsa:1124.

UCSC

uc003szz.1. human.

Organism-specific databases

CTD

1124.

GeneCards

GC07P029186.

H-InvDB

HIX0006558.

HGNC

HGNC:1944. CHN2.

HPA

HPA018989.

MIM

602857. gene.

neXtProt

NX_P52757.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00600000084231.

HOVERGEN

HBG080489.

InParanoid

P52757.

OMA

XFGNQTL.

OrthoDB

EOG4W9J3S.

PhylomeDB

P52757.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

P52757.

Bgee

P52757.

CleanEx

HS_CHN2.

Genevestigator

P52757.

GermOnline

ENSG00000106069. Homo sapiens.

Family and domain databases

InterPro

IPR020454. DAG/PE-bd.
IPR017356. N-chimaerin.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR000980. SH2.
[Graphical view]

Gene3D

G3DSA:1.10.555.10. RhoGAP. 1 hit.
G3DSA:3.30.505.10. SH2. 1 hit.

Pfam

PF00130. C1_1. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]

PIRSF

PIRSF038015. N-chimaerin. 1 hit.

PRINTS

PR00008. DAGPEDOMAIN.

SMART

SM00109. C1. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]

SUPFAM

SSF48350. Rho_GAP. 1 hit.

PROSITE

PS50238. RHOGAP. 1 hit.
PS50001. SH2. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

4668.

SOURCE

Search...

Entry information

Entry name

CHIO_HUMAN

Accession

Primary (citable) accession number: P52757
Secondary accession number(s): Q2M203, Q75MM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.