Reviewed,
UniProtKB/Swiss-Prot P52756 (RBM5_HUMAN)
Last modified
November 3, 2009.
Version 95.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: RNA-binding protein 5 Alternative name(s): RNA-binding motif protein 5 Putative tumor suppressor LUCA15 Protein G15 Renal carcinoma antigen NY-REN-9 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 815 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis. Ref.5 Ref.10 Ref.11 Ref.12 Ref.13 Ref.18 Ref.20 |
| Subunit structure | Component of the spliceosome A complex (also known as the prespliceosome). Appears to dissociate from the spliceosome upon formation of the spliceosome B complex (also known as the precatalytic spliceosome), in which the heterotrimeric U4/U6.U5 snRNPs are bound. Interacts with U2AF2; this interaction is direct. Also interacts with ACIN1, PRPF8, SFRS3, SNRPB, SNRPN, SNRNP70 and SNRNP200; these interactions may be indirect. Ref.18 |
| Subcellular location | |
| Tissue specificity | Isoform 5 is widely expressed in normal tissues and is expressed at increased levels in T-leukaemic cell lines. |
| Sequence similarities | Belongs to the RBM5/RBM10 family. Contains 1 C2H2-type zinc finger. Contains 1 G-patch domain. Contains 1 RanBP2-type zinc finger. Contains 2 RRM (RNA recognition motif) domains. |
| Sequence caution | The sequence BAG59728.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence BAG59871.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence BAG63975.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. |
Ontologies
Alternative products
| This entry describes 5 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P52756-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P52756-2) The sequence of this isoform differs from the canonical sequence as follows: 138-143: VSRGFA → ESLLSS 144-815: Missing. | ||||||
| Isoform 3 (identifier: P52756-3) The sequence of this isoform differs from the canonical sequence as follows: 65-126: ERRNSDRSED...MMESFEGPQP → YSRNDGVLRR...DSRKAHCNAL 127-815: Missing. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform 4 (identifier: P52756-4) The sequence of this isoform differs from the canonical sequence as follows: 142-815: Missing. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform 5 (identifier: P52756-5) Also known as: delta-6; The sequence of this isoform differs from the canonical sequence as follows: 137-150: GVSRGFAFVEFYHL → EKVGDSRKAHCNAL 151-815: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 815 | 815 | RNA-binding protein 5 | PRO_0000081759 | |||||
Regions | |||||||||
| Domain | 98 – 178 | 81 | RRM 1 | ||||||
| Domain | 231 – 315 | 85 | RRM 2 | ||||||
| Domain | 743 – 789 | 47 | G-patch | ||||||
| Zinc finger | 181 – 210 | 30 | RanBP2-type | ||||||
| Zinc finger | 647 – 677 | 31 | C2H2-type; atypical | ||||||
| Region | 321 – 809 | 489 | Required for interaction with U2AF2 | ||||||
| Region | 452 – 535 | 84 | Sufficient for interaction with ACIN1, PRPF8, SFRS3, SNRPB, SNRPN, SNRNP70 and SNRNP200 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 59 | 1 | Phosphoserine Ref.14 | ||||||
| Modified residue | 69 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 78 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 542 | 1 | N6-acetyllysine Ref.22 | ||||||
| Modified residue | 621 | 1 | Phosphoserine Ref.17 Ref.19 | ||||||
| Modified residue | 624 | 1 | Phosphoserine Ref.14 Ref.17 Ref.19 Ref.16 | ||||||
| Modified residue | 712 | 1 | Phosphotyrosine Ref.17 | ||||||
Natural variations | |||||||||
| Alternative sequence | 65 – 126 | 62 | ERRNS…EGPQP → YSRNDGVLRRPSACGCEADE EENRCKPWFRLRGVLSLARC YQLDGSQSEKVGDSRKAHCN AL in isoform 3. | VSP_037429 | |||||
| Alternative sequence | 127 – 815 | 689 | Missing in isoform 3. | VSP_037430 | |||||
| Alternative sequence | 137 – 150 | 14 | GVSRG…EFYHL → EKVGDSRKAHCNAL in isoform 5. | VSP_037431 | |||||
| Alternative sequence | 138 – 143 | 6 | VSRGFA → ESLLSS in isoform 2. | VSP_037432 | |||||
| Alternative sequence | 142 – 815 | 674 | Missing in isoform 4. | VSP_037433 | |||||
| Alternative sequence | 144 – 815 | 672 | Missing in isoform 2. | VSP_037434 | |||||
| Alternative sequence | 151 – 815 | 665 | Missing in isoform 5. | VSP_037435 | |||||
Experimental info | |||||||||
| Sequence conflict | 53 – 54 | 2 | DY → GS in AAA99715. Ref.1 | ||||||
| Sequence conflict | 354 | 1 | G → V in AAA99715. Ref.1 | ||||||
| Sequence conflict | 455 | 1 | Missing in AAB42216. Ref.8 | ||||||
| Sequence conflict | 788 | 1 | G → A in AAB42216. Ref.8 | ||||||
| Sequence conflict | 812 | 1 | T → I in AAA99715. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A putative tumor suppressor gene LUCA15 on 3p21.3 encodes two RNA recognizing motifs and is related to the Drosophila tumor suppresorgene Sxl." Bader S., Latif F., Duh F.-M., Wei M., Kashuba V., Sekido Y., Lee C., Koonin E., Zabarofsky E., Klein G., Minna J.D., Lerman M.I. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [2] | "An evolutionary rearrangement of the Xp11.3-11.23 region in 3p21.3, a region frequently deleted in a variety of cancers." Timmer T., Terpstra P., van den Berg A., Veldhuis P.M.J.F., Ter Elst A., van der Veen A.Y., Kok K., Naylor S.L., Buys C.H.C.M. Genomics 60:238-240(1999) [PubMed: 10486216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain. |
| [3] | "Identification of differentially expressed genes associated with HER-2/neu overexpression in human breast cancer cells." Oh J.J., Grosshans D.R., Wong S.G., Slamon D.J. Nucleic Acids Res. 27:4008-4017(1999) [PubMed: 10497265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain. |
| [4] | Oh J.J., Wong S.G., Slamon D.J. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 61. Tissue: Testis. |
| [5] | "LUCA-15-encoded sequence variants regulate CD95-mediated apoptosis." Sutherland L.C., Edwards S.E., Cable H.C., Poirier G.G., Miller B.A., Cooper C.S., Williams G.T. Oncogene 19:3774-3781(2000) [PubMed: 10949932] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING, FUNCTION. |
| [6] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3; 4 AND 5). Tissue: Placenta. |
| [7] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [8] | Bentley D., Maggi L. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 117-815. |
| [9] | "Antigens recognized by autologous antibody in patients with renal-cell carcinoma." Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J. Int. J. Cancer 83:456-464(1999) [PubMed: 10508479] [Abstract] Cited for: IDENTIFICATION AS A RENAL CANCER ANTIGEN. Tissue: Renal cell carcinoma. |
| [10] | "Candidate tumour suppressor LUCA-15 can regulate multiple apoptotic pathways." Mourtada-Maarabouni M., Sutherland L.C., Williams G.T. Apoptosis 7:421-432(2002) [PubMed: 12207175] [Abstract] Cited for: FUNCTION. |
| [11] | "Simultaneous acceleration of the cell cycle and suppression of apoptosis by splice variant delta-6 of the candidate tumour suppressor LUCA-15/RBM5." Mourtada-Maarabouni M., Sutherland L.C., Meredith J.M., Williams G.T. Genes Cells 8:109-119(2003) [PubMed: 12581154] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORM 5), FUNCTION. |
| [12] | "LUCA-15/RBM5, a putative tumour suppressor, enhances multiple receptor-initiated death signals." Rintala-Maki N.D., Sutherland L.C. Apoptosis 9:475-484(2004) [PubMed: 15192330] [Abstract] Cited for: FUNCTION. |
| [13] | "3p21.3 tumor suppressor gene H37/Luca15/RBM5 inhibits growth of human lung cancer cells through cell cycle arrest and apoptosis." Oh J.J., Razfar A., Delgado I., Reed R.A., Malkina A., Boctor B., Slamon D.J. Cancer Res. 66:3419-3427(2006) [PubMed: 16585163] [Abstract] Cited for: FUNCTION. |
| [14] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59 AND SER-624, MASS SPECTROMETRY. Tissue: Epithelium. |
| [15] | "Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes." Behzadnia N., Golas M.M., Hartmuth K., Sander B., Kastner B., Deckert J., Dube P., Will C.L., Urlaub H., Stark H., Luehrmann R. EMBO J. 26:1737-1748(2007) [PubMed: 17332742] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL A COMPLEX. |
| [16] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-624, MASS SPECTROMETRY. |
| [17] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-624 AND TYR-712, MASS SPECTROMETRY. |
| [18] | "RBM5/Luca-15/H37 regulates Fas alternative splice site pairing after exon definition." Bonnal S., Martinez C., Foerch P., Bachi A., Wilm M., Valcarcel J. Mol. Cell 32:81-95(2008) [PubMed: 18851835] [Abstract] Cited for: FUNCTION, INTERACTION WITH ACIN1; PRPF8; SFRS3; SNRPB; SNRPN; SNRNP70; SNRNP200 AND U2AF2, SUBCELLULAR LOCATION. |
| [19] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621 AND SER-624, MASS SPECTROMETRY. |
| [20] | "Up-regulation of the proapoptotic caspase 2 splicing isoform by a candidate tumor suppressor, RBM5." Fushimi K., Ray P., Kar A., Wang L., Sutherland L.C., Wu J.Y. Proc. Natl. Acad. Sci. U.S.A. 105:15708-15713(2008) [PubMed: 18840686] [Abstract] Cited for: FUNCTION, RNA-BINDING. |
| [21] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [22] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-542, MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| U23946 mRNA. Translation: AAA99715.1. AF091263 mRNA. Translation: AAD04159.1. AF103802 mRNA. Translation: AAF02422.2. AF107493 mRNA. Translation: AAF99551.1. AK297249 mRNA. Translation: BAG59728.1. Sequence problems. AK297445 mRNA. Translation: BAG59871.1. Sequence problems. AK302766 mRNA. Translation: BAG63975.1. Sequence problems. AK314032 mRNA. Translation: BAG36742.1. CH471055 Genomic DNA. Translation: EAW65040.1. CH471055 Genomic DNA. Translation: EAW65041.1. U73168 Genomic DNA. Translation: AAB42216.1. | |
| IPI | IPI00005036. IPI00796867. IPI00927051. IPI00930333. IPI00930619. |
| RefSeq | NP_005769.1. |
| UniGene | Hs.439480 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P52756. 1 interaction. |
| STRING | P52756. |
PTM databases | |
| PhosphoSite | P52756. |
Proteomic databases | |
| PRIDE | P52756. |
Genome annotation databases | |
| Ensembl | ENST00000347869; ENSP00000343054; ENSG00000003756; Homo sapiens. [Genome view] ENST00000395174; ENSP00000378603; ENSG00000003756; Homo sapiens. [Genome view] ENST00000404526; ENSP00000384872; ENSG00000003756; Homo sapiens. [Genome view] ENST00000417905; ENSP00000406119; ENSG00000003756; Homo sapiens. [Genome view] ENST00000433556; ENSP00000396926; ENSG00000003756; Homo sapiens. [Genome view] ENST00000437500; ENSP00000394622; ENSG00000003756; Homo sapiens. [Genome view] ENST00000438369; ENSP00000414218; ENSG00000003756; Homo sapiens. [Genome view] ENST00000441305; ENSP00000390711; ENSG00000003756; Homo sapiens. [Genome view] ENST00000441812; ENSP00000398426; ENSG00000003756; Homo sapiens. [Genome view] |
| GeneID | 10181. |
| KEGG | hsa:10181. |
| UCSC | uc003cyf.2. human. uc003cyg.1. human. |
Organism-specific databases | |
| CTD | 10181. |
| GeneCards | GC03P050101. |
| HGNC | HGNC:9902. RBM5. |
| HPA | HPA017335. HPA018011. |
| MIM | 606884. gene. |
| PharmGKB | PA34267. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | P52756. |
| HOVERGEN | P52756. |
| OMA | SHQQAGL. |
Enzyme and pathway databases | |
| Reactome | REACT_125. Processing of Capped Intron-Containing Pre-mRNA. REACT_6167. Influenza Infection. REACT_71. Gene Expression. |
Gene expression databases | |
| ArrayExpress | P52756. |
| Bgee | P52756. |
| Genevestigator | P52756. |
| GermOnline | ENSG00000003756. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR012677. a_b_plait_nuc_bd. IPR000467. G_patch. IPR000504. RRM_RNP1. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR001876. Znf_RanBP2. [Graphical view] |
| Gene3D | G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits. |
| Pfam | PF01585. G-patch. 1 hit. PF00076. RRM_1. 1 hit. PF00641. zf-RanBP. 1 hit. [Graphical view] |
| SMART | SM00443. G_patch. 1 hit. SM00360. RRM. 2 hits. SM00355. ZnF_C2H2. 1 hit. SM00547. ZnF_RBZ. 1 hit. [Graphical view] |
| PROSITE | PS50174. G_PATCH. 1 hit. PS50102. RRM. 2 hits. PS01358. ZF_RANBP2_1. 1 hit. PS50199. ZF_RANBP2_2. 1 hit. PS00028. ZINC_FINGER_C2H2_1. False negative. PS50157. ZINC_FINGER_C2H2_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 38542. |
| SOURCE | Search... |
Entry information
| Entry name | RBM5_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P52756 Secondary accession number(s): B2RA45 Q9UL24 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 3 Human chromosome 3: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


