Reviewed, UniProtKB/Swiss-Prot P52756 (RBM5_HUMAN)

Names and origin

Protein names

Recommended name:
    RNA-binding protein 5
Alternative name(s):
    RNA-binding motif protein 5
    Tumor suppressor LUCA15
    Protein G15
    Renal carcinoma antigen NY-REN-9

Gene names

Name:	RBM5
ORF Names:	H37, LUCA15

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tumor suppressor protein that reduces cell proliferation and promotes apoptosis and cell cycle arrest.
Subcellular location	NucleusPotential.
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 G-patch domain. Contains 1 RanBP2-type zinc finger. Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Molecular function	Anti-oncogene
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	negative regulation of cell cycle Inferred from electronic annotation. Source: UniProtKB-KW nuclear mRNA splicing, via spliceosome Inferred from Experiment. Source: Reactome
Cellular component	nucleus Traceable author statement. Source: ProtInc
Molecular function	DNA binding Traceable author statement. Source: ProtInc RNA binding Traceable author statement. Source: ProtInc nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 815	815	RNA-binding protein 5		PRO_0000081759
Regions
	Domain	98 – 178	81	RRM 1
	Domain	231 – 315	85	RRM 2
	Domain	743 – 789	47	G-patch
	Zinc finger	181 – 210	30	RanBP2-type
	Zinc finger	647 – 677	31	C2H2-type; atypical
Amino acid modifications
	Modified residue	59	1	Phosphoserine
	Modified residue	69	1	Phosphoserine By similarity
	Modified residue	621	1	Phosphoserine
	Modified residue	624	1	Phosphoserine
Experimental info
	Sequence conflict	53 – 54	2	DY → GS in AAA99715. Ref.1
	Sequence conflict	354	1	G → V in AAA99715. Ref.1
	Sequence conflict	455	1	Missing in AAB42216. Ref.5
	Sequence conflict	788	1	G → A in AAB42216. Ref.5
	Sequence conflict	812	1	T → I in AAA99715. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52756-1 [UniParc]. Last modified February 21, 2001. Version 2. Checksum: AA79962D13405479	FASTA	815	92,154
	10 20 30 40 50 60 MGSDKRVSRT ERSGRYGSII DRDDRDERES RSRRRDSDYK RSSDDRRGDR YDDYRDYDSP 70 80 90 100 110 120 ERERERRNSD RSEDGYHSDG DYGEHDYRHD ISDERESKTI MLRGLPITIT ESDIREMMES 130 140 150 160 170 180 FEGPQPADVR LMKRKTGVSR GFAFVEFYHL QDATSWMEAN QKKLVIQGKH IAMHYSNPRP 190 200 210 220 230 240 KFEDWLCNKC CLNNFRKRLK CFRCGADKFD SEQEVPPGTT ESVQSVDYYC DTIILRNIAP 250 260 270 280 290 300 HTVVDSIMTA LSPYASLAVN NIRLIKDKQT QQNRGFAFVQ LSSAMDASQL LQILQSLHPP 310 320 330 340 350 360 LKIDGKTIGV DFAKSARKDL VLSDGNRVSA FSVASTAIAA AQWSSTQSQS GEGGSVDYSY 370 380 390 400 410 420 LQPGQDGYAQ YAQYSQDYQQ FYQQQAGGLE SDASSASGTA VTTTSAAVVS QSPQLYNQTS 430 440 450 460 470 480 NPPGSPTEEA QPSTSTSTQA PAASPTGVVP GTKYAVPDTS TYQYDESSGY YYDPTTGLYY 490 500 510 520 530 540 DPNSQYYYNS LTQQYLYWDG EKETYVPAAE SSSHQQSGLP PAKEGKEKKE KPKSKTAQQI 550 560 570 580 590 600 AKDMERWAKS LNKQKENFKN SFQPVNSLRE EERRESAAAD AGFALFEKKG ALAERQQLIP 610 620 630 640 650 660 ELVRNGDEEN PLKRGLVAAY SGDSDNEEEL VERLESEEEK LADWKKMACL LCRRQFPNKD 670 680 690 700 710 720 ALVRHQQLSD LHKQNMDIYR RSRLSEQELE ALELREREMK YRDRAAERRE KYGIPEPPEP 730 740 750 760 770 780 KRKKQFDAGT VNYEQPTKDG IDHSNIGNKM LQAMGWREGS GLGRKCQGIT APIEAQVRLK 790 800 810 GAGLGAKGSA YGLSGADSYK DAVRKAMFAR FTEME

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A putative tumor suppressor gene LUCA15 on 3p21.3 encodes two RNA recognizing motifs and is related to the Drosophila tumor suppresorgene Sxl." Bader S., Latif F., Duh F.-M., Wei M., Kashuba V., Sekido Y., Lee C., Koonin E., Zabarofsky E., Klein G., Minna J.D., Lerman M.I. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"An evolutionary rearrangement of the Xp11.3-11.23 region in 3p21.3, a region frequently deleted in a variety of cancers." Timmer T., Terpstra P., van den Berg A., Veldhuis P.M.J.F., Ter Elst A., van der Veen A.Y., Kok K., Naylor S.L., Buys C.H.C.M. Genomics 60:238-240(1999) [PubMed: 10486216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Identification of differentially expressed genes associated with HER-2/neu overexpression in human breast cancer cells." Oh J.J., Grosshans D.R., Wong S.G., Slamon D.J. Nucleic Acids Res. 27:4008-4017(1999) [PubMed: 10497265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	Oh J.J., Wong S.G., Slamon D.J. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 61.
[5]	Bentley D., Maggi L. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 117-815.
[6]	"Antigens recognized by autologous antibody in patients with renal-cell carcinoma." Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J. Int. J. Cancer 83:456-464(1999) [PubMed: 10508479] [Abstract] Cited for: IDENTIFICATION AS A RENAL CANCER ANTIGEN. Tissue: Renal cell carcinoma.
[7]	"3p21.3 tumor suppressor gene H37/Luca15/RBM5 inhibits growth of human lung cancer cells through cell cycle arrest and apoptosis." Oh J.J., Razfar A., Delgado I., Reed R.A., Malkina A., Boctor B., Slamon D.J. Cancer Res. 66:3419-3427(2006) [PubMed: 16585163] [Abstract] Cited for: FUNCTION.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59 AND SER-624, MASS SPECTROMETRY. Tissue: Epithelium.
[9]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-624, MASS SPECTROMETRY.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621 AND SER-624, MASS SPECTROMETRY.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23946 mRNA. Translation: AAA99715.1. AF091263 mRNA. Translation: AAD04159.1. AF103802 mRNA. Translation: AAF02422.2. U73168 Genomic DNA. Translation: AAB42216.1.
RefSeq	NP_005769.1.
UniGene	Hs.439480
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P52756.
PTM databases
PhosphoSite	P52756.
Genome annotation databases
Ensembl	ENSG00000003756. Homo sapiens. [Contig view]
GeneID	10181.
KEGG	hsa:10181.
Organism-specific databases
HGNC	HGNC:9902. RBM5.
HPA	HPA017335. HPA018011.
MIM	606884. gene.
PharmGKB	PA34267.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P52756.
HOVERGEN	P52756.
Enzyme and pathway databases
Reactome	REACT_1675. mRNA Processing. REACT_6167. Influenza Infection. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P52756.
GermOnline	ENSG00000003756. Homo sapiens.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR000467. G_patch. IPR000504. RRM_RNP1. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR001876. Znf_RanBP2. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
Pfam	PF01585. G-patch. 1 hit. PF00076. RRM_1. 1 hit. PF00641. zf-RanBP. 1 hit. [Graphical view]
SMART	SM00443. G_patch. 1 hit. SM00360. RRM. 2 hits. SM00355. ZnF_C2H2. 1 hit. SM00547. ZnF_RBZ. 1 hit. [Graphical view]
PROSITE	PS50174. G_PATCH. 1 hit. PS50102. RRM. 2 hits. PS01358. ZF_RANBP2_1. 1 hit. PS50199. ZF_RANBP2_2. 1 hit. PS00028. ZINC_FINGER_C2H2_1. False negative. PS50157. ZINC_FINGER_C2H2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	38542.
SOURCE	Search...

Entry information

Entry name

RBM5_HUMAN

Accession

Primary (citable) accession number: P52756
Secondary accession number(s): Q93021 , Q9BU14, Q9UKY8, Q9UL24

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	February 21, 2001
Last modified:	November 25, 2008
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)