P52747 (ZN143_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 143

Alternative name(s):
SPH-binding factor
Selenocysteine tRNA gene transcription-activating factor
Short name=hStaf

Gene names

Name:	ZNF143
Synonyms:	SBF, STAF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8. Ref.5 Ref.6
Subunit structure	Interacts with CHD8. Ref.6
Subcellular location	Nucleus Potential.
Tissue specificity	Expressed in all tissues tested, with the strongest expression in ovary.
Sequence similarities	Belongs to the GLI C2H2-type zinc-finger protein family. Contains 7 C2H2-type zinc fingers.
Sequence caution	The sequence AAC50266.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH20219.1 differs from that shown. Reason: Erroneous initiation. The sequence BAF83822.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription from RNA polymerase II promoter Traceable author statement PubMed 9566884. Source: ProtInc regulation of transcription from RNA polymerase III promoter Traceable author statement PubMed 9566884. Source: ProtInc transcription from RNA polymerase II promoter Traceable author statement PubMed 9009278. Source: ProtInc transcription from RNA polymerase III promoter Traceable author statement. Source: Reactome
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P52747-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P52747-2)

The sequence of this isoform differs from the canonical sequence as follows:
     39-69: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 638	638	Zinc finger protein 143		PRO_0000047426
Regions
	Zinc finger	237 – 261	25	C2H2-type 1
	Zinc finger	267 – 291	25	C2H2-type 2
	Zinc finger	297 – 321	25	C2H2-type 3
	Zinc finger	327 – 351	25	C2H2-type 4
	Zinc finger	357 – 381	25	C2H2-type 5
	Zinc finger	387 – 411	25	C2H2-type 6
	Zinc finger	417 – 440	24	C2H2-type 7
Natural variations
	Alternative sequence	39 – 69	31	Missing in isoform 2.		VSP_036978
	Natural variant	461	1	G → D. Corresponds to variant rs34972213 [ dbSNP | Ensembl ].		VAR_061937
	Natural variant	561	1	E → Q. Ref.1 Ref.3 Ref.5 Corresponds to variant rs10743108 [ dbSNP | Ensembl ].		VAR_027254
Experimental info
	Sequence conflict	433	1	A → V in AAH20219. Ref.3
	Sequence conflict	590	1	Missing in BAG59697. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: D149030016A6058A	FASTA	638	68,896
	10 20 30 40 50 60 MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL QAVTLADGST 70 80 90 100 110 120 AYIQHNSKDA KLIDGQVIQL EDGSAAYVQH VPIPKSTGDS LRLEDGQAVQ LEDGTTAFIH 130 140 150 160 170 180 HTSKDSYDQS ALQAVQLEDG TTAYIHHAVQ VPQSDTILAI QADGTVAGLH TGDATIDPDT 190 200 210 220 230 240 ISALEQYAAK VSIDGSESVA GTGMIGENEQ EKKMQIVLQG HATRVTAKSQ QSGEKAFRCE 250 260 270 280 290 300 YDGCGKLYTT AHHLKVHERS HTGDRPYQCE HAGCGKAFAT GYGLKSHVRT HTGEKPYRCS 310 320 330 340 350 360 EDNCTKSFKT SGDLQKHIRT HTGERPFKCP FEGCGRSFTT SNIRKVHVRT HTGERPYYCT 370 380 390 400 410 420 EPGCGRAFAS ATNYKNHVRI HTGEKPYVCT VPGCDKRFTE YSSLYKHHVV HTHSKPYNCN 430 440 450 460 470 480 HCGKTYKQIS TLAMHKRTAH NDTEPIEEEQ EAFFEPPPGQ GEDVLKGSQI TYVTGVEGDD 490 500 510 520 530 540 VVSTQVATVT QSGLSQQVTL ISQDGTQHVN ISQADMQAIG NTITMVTQDG TPITVPAHDA 550 560 570 580 590 600 VISSAGTHSV AMVTAEGTEG EQVAIVAQDL AAFHTASSEM GHQQHSHHLV TTETRPLTLV 610 620 630 ATSNGTQIAV QLGEQPSLEE AIRIASRIQQ GETPGLDD
	Isoform 2 [UniParc]. Checksum: 285B599E182261D6	FASTA	607	65,636
	10 20 30 40 50 60 MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADAK LIDGQVIQLE DGSAAYVQHV 70 80 90 100 110 120 PIPKSTGDSL RLEDGQAVQL EDGTTAFIHH TSKDSYDQSA LQAVQLEDGT TAYIHHAVQV 130 140 150 160 170 180 PQSDTILAIQ ADGTVAGLHT GDATIDPDTI SALEQYAAKV SIDGSESVAG TGMIGENEQE 190 200 210 220 230 240 KKMQIVLQGH ATRVTAKSQQ SGEKAFRCEY DGCGKLYTTA HHLKVHERSH TGDRPYQCEH 250 260 270 280 290 300 AGCGKAFATG YGLKSHVRTH TGEKPYRCSE DNCTKSFKTS GDLQKHIRTH TGERPFKCPF 310 320 330 340 350 360 EGCGRSFTTS NIRKVHVRTH TGERPYYCTE PGCGRAFASA TNYKNHVRIH TGEKPYVCTV 370 380 390 400 410 420 PGCDKRFTEY SSLYKHHVVH THSKPYNCNH CGKTYKQIST LAMHKRTAHN DTEPIEEEQE 430 440 450 460 470 480 AFFEPPPGQG EDVLKGSQIT YVTGVEGDDV VSTQVATVTQ SGLSQQVTLI SQDGTQHVNI 490 500 510 520 530 540 SQADMQAIGN TITMVTQDGT PITVPAHDAV ISSAGTHSVA MVTAEGTEGE QVAIVAQDLA 550 560 570 580 590 600 AFHTASSEMG HQQHSHHLVT TETRPLTLVA TSNGTQIAVQ LGEQPSLEEA IRIASRIQQG ETPGLDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT GLN-561. Tissue: Brain.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLN-561. Tissue: Muscle.
[4]	"Isolation and fine mapping of 16 novel human zinc finger-encoding cDNAs identify putative candidate genes for developmental and malignant disorders." Tommerup N., Vissing H. Genomics 27:259-264(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-638 (ISOFORM 1). Tissue: Insulinoma.
[5]	"Molecular cloning of a cDNA encoding human SPH-binding factor, a conserved protein that binds to the enhancer-like region of the U6 small nuclear RNA gene promoter." Rincon J.C., Engler S.K., Hargrove B.W., Kunkel G.R. Nucleic Acids Res. 26:4846-4852(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 88-638 (ISOFORMS 1/2), VARIANT GLN-561, FUNCTION.
[6]	"CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription." Yuan C.-C., Zhao X., Florens L., Swanson S.K., Washburn M.P., Hernandez N. Mol. Cell. Biol. 27:8729-8738(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CHD8.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK291133 mRNA. Translation: BAF83822.1. Different initiation. AK297214 mRNA. Translation: BAG59697.1. AC127030 Genomic DNA. No translation available. AC132192 Genomic DNA. No translation available. BC020219 mRNA. Translation: AAH20219.1. Different initiation. U09850 mRNA. Translation: AAC50266.1. Different initiation. AF071771 mRNA. Translation: AAC96102.1.
IPI	IPI00305316. IPI00929121.
PIR	I38618.
RefSeq	NP_003433.3. NM_003442.5.
UniGene	Hs.523471.
3D structure databases
ProteinModelPortal	P52747.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52747. 1 interaction.
STRING	9606.ENSP00000379847.
PTM databases
PhosphoSite	P52747.
Polymorphism databases
DMDM	229462806.
Proteomic databases
PaxDb	P52747.
PRIDE	P52747.
Protocols and materials databases
DNASU	7702.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000396597; ENSP00000379843; ENSG00000166478. ENST00000396602; ENSP00000379847; ENSG00000166478.
GeneID	7702.
KEGG	hsa:7702.
UCSC	uc001mhr.3. human. uc010rby.2. human.
Organism-specific databases
CTD	7702.
GeneCards	GC11P009483.
HGNC	HGNC:12928. ZNF143.
HPA	HPA003263.
MIM	603433. gene.
neXtProt	NX_P52747.
PharmGKB	PA37515.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000118073.
HOVERGEN	HBG053078.
InParanoid	P52747.
OMA	QEKKMQI.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P52747.
Bgee	P52747.
CleanEx	HS_ZNF143.
Genevestigator	P52747.
GermOnline	ENSG00000166478. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 7 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 7 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 7 hits. PS50157. ZINC_FINGER_C2H2_2. 7 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ZNF143. human.
GenomeRNAi	7702.
NextBio	29854.
SOURCE	Search...

Entry information

Entry name

ZN143_HUMAN

Accession

Primary (citable) accession number: P52747
Secondary accession number(s): A8K518 , B4DLY5, O75559, Q8WUK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.