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Protein

Zinc finger protein 143

Gene

ZNF143

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri237 – 26125C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri267 – 29125C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri297 – 32125C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri327 – 35125C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri357 – 38125C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri387 – 41125C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri417 – 44024C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 143
Alternative name(s):
SPH-binding factor
Selenocysteine tRNA gene transcription-activating factor
Short name:
hStaf
Gene namesi
Name:ZNF143
Synonyms:SBF, STAF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:12928. ZNF143.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37515.

Polymorphism and mutation databases

BioMutaiZNF143.
DMDMi229462806.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 638638Zinc finger protein 143PRO_0000047426Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP52747.
PaxDbiP52747.
PRIDEiP52747.

PTM databases

PhosphoSiteiP52747.

Expressioni

Tissue specificityi

Expressed in all tissues tested, with the strongest expression in ovary.

Gene expression databases

BgeeiP52747.
CleanExiHS_ZNF143.
ExpressionAtlasiP52747. baseline and differential.
GenevisibleiP52747. HS.

Organism-specific databases

HPAiHPA003263.

Interactioni

Subunit structurei

Interacts with CHD8.1 Publication

Protein-protein interaction databases

BioGridi113496. 3 interactions.
IntActiP52747. 1 interaction.
STRINGi9606.ENSP00000379847.

Structurei

3D structure databases

ProteinModelPortaliP52747.
SMRiP52747. Positions 231-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri237 – 26125C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri267 – 29125C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri297 – 32125C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri327 – 35125C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri357 – 38125C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri387 – 41125C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri417 – 44024C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000118073.
HOVERGENiHBG053078.
InParanoidiP52747.
OMAiKMQIVLQ.
OrthoDBiEOG7WX080.
PhylomeDBiP52747.
TreeFamiTF333498.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 7 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P52747-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL
60 70 80 90 100
QAVTLADGST AYIQHNSKDA KLIDGQVIQL EDGSAAYVQH VPIPKSTGDS
110 120 130 140 150
LRLEDGQAVQ LEDGTTAFIH HTSKDSYDQS ALQAVQLEDG TTAYIHHAVQ
160 170 180 190 200
VPQSDTILAI QADGTVAGLH TGDATIDPDT ISALEQYAAK VSIDGSESVA
210 220 230 240 250
GTGMIGENEQ EKKMQIVLQG HATRVTAKSQ QSGEKAFRCE YDGCGKLYTT
260 270 280 290 300
AHHLKVHERS HTGDRPYQCE HAGCGKAFAT GYGLKSHVRT HTGEKPYRCS
310 320 330 340 350
EDNCTKSFKT SGDLQKHIRT HTGERPFKCP FEGCGRSFTT SNIRKVHVRT
360 370 380 390 400
HTGERPYYCT EPGCGRAFAS ATNYKNHVRI HTGEKPYVCT VPGCDKRFTE
410 420 430 440 450
YSSLYKHHVV HTHSKPYNCN HCGKTYKQIS TLAMHKRTAH NDTEPIEEEQ
460 470 480 490 500
EAFFEPPPGQ GEDVLKGSQI TYVTGVEGDD VVSTQVATVT QSGLSQQVTL
510 520 530 540 550
ISQDGTQHVN ISQADMQAIG NTITMVTQDG TPITVPAHDA VISSAGTHSV
560 570 580 590 600
AMVTAEGTEG EQVAIVAQDL AAFHTASSEM GHQQHSHHLV TTETRPLTLV
610 620 630
ATSNGTQIAV QLGEQPSLEE AIRIASRIQQ GETPGLDD
Length:638
Mass (Da):68,896
Last modified:May 5, 2009 - v2
Checksum:iD149030016A6058A
GO
Isoform 2 (identifier: P52747-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     39-69: Missing.

Show »
Length:607
Mass (Da):65,636
Checksum:i285B599E182261D6
GO
Isoform 3 (identifier: P52747-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-98: TG → R

Note: No experimental confirmation available.
Show »
Length:637
Mass (Da):68,894
Checksum:iF393F46093039A3C
GO

Sequence cautioni

The sequence AAC50266.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH20219.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAF83822.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti433 – 4331A → V in AAH20219 (PubMed:15489334).Curated
Sequence conflicti443 – 4431T → A in AK313330 (PubMed:14702039).Curated
Sequence conflicti590 – 5901Missing in BAG59697 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti461 – 4611G → D.
Corresponds to variant rs34972213 [ dbSNP | Ensembl ].
VAR_061937
Natural varianti561 – 5611E → Q.3 Publications
Corresponds to variant rs10743108 [ dbSNP | Ensembl ].
VAR_027254

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei39 – 6931Missing in isoform 2. 1 PublicationVSP_036978Add
BLAST
Alternative sequencei97 – 982TG → R in isoform 3. 1 PublicationVSP_055109

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK291133 mRNA. Translation: BAF83822.1. Different initiation.
AK297214 mRNA. Translation: BAG59697.1.
AK313330 mRNA. No translation available.
AC127030 Genomic DNA. No translation available.
AC132192 Genomic DNA. No translation available.
BC020219 mRNA. Translation: AAH20219.1. Different initiation.
U09850 mRNA. Translation: AAC50266.1. Different initiation.
AF071771 mRNA. Translation: AAC96102.1.
CCDSiCCDS60720.1. [P52747-3]
CCDS60721.1. [P52747-2]
CCDS7799.2. [P52747-1]
PIRiI38618.
RefSeqiNP_001269585.1. NM_001282656.1. [P52747-3]
NP_001269586.1. NM_001282657.1. [P52747-2]
NP_003433.3. NM_003442.5. [P52747-1]
XP_011518651.1. XM_011520349.1. [P52747-1]
UniGeneiHs.523471.

Genome annotation databases

EnsembliENST00000396597; ENSP00000379843; ENSG00000166478. [P52747-2]
ENST00000396602; ENSP00000379847; ENSG00000166478.
ENST00000396604; ENSP00000379849; ENSG00000166478. [P52747-3]
ENST00000530463; ENSP00000432154; ENSG00000166478. [P52747-3]
GeneIDi7702.
KEGGihsa:7702.
UCSCiuc001mhr.3. human. [P52747-1]
uc010rby.2. human. [P52747-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK291133 mRNA. Translation: BAF83822.1. Different initiation.
AK297214 mRNA. Translation: BAG59697.1.
AK313330 mRNA. No translation available.
AC127030 Genomic DNA. No translation available.
AC132192 Genomic DNA. No translation available.
BC020219 mRNA. Translation: AAH20219.1. Different initiation.
U09850 mRNA. Translation: AAC50266.1. Different initiation.
AF071771 mRNA. Translation: AAC96102.1.
CCDSiCCDS60720.1. [P52747-3]
CCDS60721.1. [P52747-2]
CCDS7799.2. [P52747-1]
PIRiI38618.
RefSeqiNP_001269585.1. NM_001282656.1. [P52747-3]
NP_001269586.1. NM_001282657.1. [P52747-2]
NP_003433.3. NM_003442.5. [P52747-1]
XP_011518651.1. XM_011520349.1. [P52747-1]
UniGeneiHs.523471.

3D structure databases

ProteinModelPortaliP52747.
SMRiP52747. Positions 231-438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113496. 3 interactions.
IntActiP52747. 1 interaction.
STRINGi9606.ENSP00000379847.

PTM databases

PhosphoSiteiP52747.

Polymorphism and mutation databases

BioMutaiZNF143.
DMDMi229462806.

Proteomic databases

MaxQBiP52747.
PaxDbiP52747.
PRIDEiP52747.

Protocols and materials databases

DNASUi7702.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000396597; ENSP00000379843; ENSG00000166478. [P52747-2]
ENST00000396602; ENSP00000379847; ENSG00000166478.
ENST00000396604; ENSP00000379849; ENSG00000166478. [P52747-3]
ENST00000530463; ENSP00000432154; ENSG00000166478. [P52747-3]
GeneIDi7702.
KEGGihsa:7702.
UCSCiuc001mhr.3. human. [P52747-1]
uc010rby.2. human. [P52747-2]

Organism-specific databases

CTDi7702.
GeneCardsiGC11P009483.
HGNCiHGNC:12928. ZNF143.
HPAiHPA003263.
MIMi603433. gene.
neXtProtiNX_P52747.
PharmGKBiPA37515.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000118073.
HOVERGENiHBG053078.
InParanoidiP52747.
OMAiKMQIVLQ.
OrthoDBiEOG7WX080.
PhylomeDBiP52747.
TreeFamiTF333498.

Enzyme and pathway databases

ReactomeiREACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

ChiTaRSiZNF143. human.
GeneWikiiZNF143.
GenomeRNAii7702.
NextBioi29854.
PROiP52747.
SOURCEiSearch...

Gene expression databases

BgeeiP52747.
CleanExiHS_ZNF143.
ExpressionAtlasiP52747. baseline and differential.
GenevisibleiP52747. HS.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 7 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3), VARIANT GLN-561.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLN-561.
    Tissue: Muscle.
  4. "Isolation and fine mapping of 16 novel human zinc finger-encoding cDNAs identify putative candidate genes for developmental and malignant disorders."
    Tommerup N., Vissing H.
    Genomics 27:259-264(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-638 (ISOFORM 1).
    Tissue: Insulinoma.
  5. "Molecular cloning of a cDNA encoding human SPH-binding factor, a conserved protein that binds to the enhancer-like region of the U6 small nuclear RNA gene promoter."
    Rincon J.C., Engler S.K., Hargrove B.W., Kunkel G.R.
    Nucleic Acids Res. 26:4846-4852(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 88-638 (ISOFORMS 1/2), VARIANT GLN-561, FUNCTION.
  6. "CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription."
    Yuan C.-C., Zhao X., Florens L., Swanson S.K., Washburn M.P., Hernandez N.
    Mol. Cell. Biol. 27:8729-8738(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CHD8.
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiZN143_HUMAN
AccessioniPrimary (citable) accession number: P52747
Secondary accession number(s): A8K518
, B4DLY5, E7ER34, O75559, Q8WUK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 5, 2009
Last modified: July 22, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.