Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P52747 (ZN143_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 143
Alternative name(s):
SPH-binding factor
Selenocysteine tRNA gene transcription-activating factor
Short name=hStaf
Gene names
Name:ZNF143
Synonyms:SBF, STAF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length638 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8. Ref.5 Ref.6

Subunit structure

Interacts with CHD8. Ref.6

Subcellular location

Nucleus Potential.

Tissue specificity

Expressed in all tissues tested, with the strongest expression in ovary.

Sequence similarities

Belongs to the GLI C2H2-type zinc-finger protein family.

Contains 7 C2H2-type zinc fingers.

Sequence caution

The sequence AAC50266.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH20219.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAF83822.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P52747-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P52747-2)

The sequence of this isoform differs from the canonical sequence as follows:
     39-69: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 638638Zinc finger protein 143
PRO_0000047426

Regions

Zinc finger237 – 26125C2H2-type 1
Zinc finger267 – 29125C2H2-type 2
Zinc finger297 – 32125C2H2-type 3
Zinc finger327 – 35125C2H2-type 4
Zinc finger357 – 38125C2H2-type 5
Zinc finger387 – 41125C2H2-type 6
Zinc finger417 – 44024C2H2-type 7

Amino acid modifications

Modified residue11N-acetylmethionine Ref.7

Natural variations

Alternative sequence39 – 6931Missing in isoform 2.
VSP_036978
Natural variant4611G → D.
Corresponds to variant rs34972213 [ dbSNP | Ensembl ].
VAR_061937
Natural variant5611E → Q. Ref.1 Ref.3 Ref.5
Corresponds to variant rs10743108 [ dbSNP | Ensembl ].
VAR_027254

Experimental info

Sequence conflict4331A → V in AAH20219. Ref.3
Sequence conflict5901Missing in BAG59697. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: D149030016A6058A

FASTA63868,896
        10         20         30         40         50         60 
MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL QAVTLADGST 

        70         80         90        100        110        120 
AYIQHNSKDA KLIDGQVIQL EDGSAAYVQH VPIPKSTGDS LRLEDGQAVQ LEDGTTAFIH 

       130        140        150        160        170        180 
HTSKDSYDQS ALQAVQLEDG TTAYIHHAVQ VPQSDTILAI QADGTVAGLH TGDATIDPDT 

       190        200        210        220        230        240 
ISALEQYAAK VSIDGSESVA GTGMIGENEQ EKKMQIVLQG HATRVTAKSQ QSGEKAFRCE 

       250        260        270        280        290        300 
YDGCGKLYTT AHHLKVHERS HTGDRPYQCE HAGCGKAFAT GYGLKSHVRT HTGEKPYRCS 

       310        320        330        340        350        360 
EDNCTKSFKT SGDLQKHIRT HTGERPFKCP FEGCGRSFTT SNIRKVHVRT HTGERPYYCT 

       370        380        390        400        410        420 
EPGCGRAFAS ATNYKNHVRI HTGEKPYVCT VPGCDKRFTE YSSLYKHHVV HTHSKPYNCN 

       430        440        450        460        470        480 
HCGKTYKQIS TLAMHKRTAH NDTEPIEEEQ EAFFEPPPGQ GEDVLKGSQI TYVTGVEGDD 

       490        500        510        520        530        540 
VVSTQVATVT QSGLSQQVTL ISQDGTQHVN ISQADMQAIG NTITMVTQDG TPITVPAHDA 

       550        560        570        580        590        600 
VISSAGTHSV AMVTAEGTEG EQVAIVAQDL AAFHTASSEM GHQQHSHHLV TTETRPLTLV 

       610        620        630 
ATSNGTQIAV QLGEQPSLEE AIRIASRIQQ GETPGLDD 

« Hide

Isoform 2 [UniParc].

Checksum: 285B599E182261D6
Show »

FASTA60765,636

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT GLN-561.
Tissue: Brain.
[2]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLN-561.
Tissue: Muscle.
[4]"Isolation and fine mapping of 16 novel human zinc finger-encoding cDNAs identify putative candidate genes for developmental and malignant disorders."
Tommerup N., Vissing H.
Genomics 27:259-264(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-638 (ISOFORM 1).
Tissue: Insulinoma.
[5]"Molecular cloning of a cDNA encoding human SPH-binding factor, a conserved protein that binds to the enhancer-like region of the U6 small nuclear RNA gene promoter."
Rincon J.C., Engler S.K., Hargrove B.W., Kunkel G.R.
Nucleic Acids Res. 26:4846-4852(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 88-638 (ISOFORMS 1/2), VARIANT GLN-561, FUNCTION.
[6]"CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription."
Yuan C.-C., Zhao X., Florens L., Swanson S.K., Washburn M.P., Hernandez N.
Mol. Cell. Biol. 27:8729-8738(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CHD8.
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK291133 mRNA. Translation: BAF83822.1. Different initiation.
AK297214 mRNA. Translation: BAG59697.1.
AC127030 Genomic DNA. No translation available.
AC132192 Genomic DNA. No translation available.
BC020219 mRNA. Translation: AAH20219.1. Different initiation.
U09850 mRNA. Translation: AAC50266.1. Different initiation.
AF071771 mRNA. Translation: AAC96102.1.
PIRI38618.
RefSeqNP_001269585.1. NM_001282656.1.
NP_001269586.1. NM_001282657.1.
NP_003433.3. NM_003442.5.
UniGeneHs.523471.

3D structure databases

ProteinModelPortalP52747.
SMRP52747. Positions 231-441.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113496. 3 interactions.
IntActP52747. 1 interaction.
STRING9606.ENSP00000379847.

PTM databases

PhosphoSiteP52747.

Polymorphism databases

DMDM229462806.

Proteomic databases

PaxDbP52747.
PRIDEP52747.

Protocols and materials databases

DNASU7702.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000396597; ENSP00000379843; ENSG00000166478. [P52747-2]
ENST00000396602; ENSP00000379847; ENSG00000166478. [P52747-1]
GeneID7702.
KEGGhsa:7702.
UCSCuc001mhr.3. human. [P52747-1]
uc010rby.2. human. [P52747-2]

Organism-specific databases

CTD7702.
GeneCardsGC11P009483.
HGNCHGNC:12928. ZNF143.
HPAHPA003263.
MIM603433. gene.
neXtProtNX_P52747.
PharmGKBPA37515.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000118073.
HOVERGENHBG053078.
InParanoidP52747.
OMAQEKKMQI.
OrthoDBEOG7WX080.
PhylomeDBP52747.
TreeFamTF333498.

Enzyme and pathway databases

ReactomeREACT_1788. Transcription.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressP52747.
BgeeP52747.
CleanExHS_ZNF143.
GenevestigatorP52747.

Family and domain databases

Gene3D3.30.160.60. 7 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 7 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZNF143. human.
GeneWikiZNF143.
GenomeRNAi7702.
NextBio29854.
PROP52747.
SOURCESearch...

Entry information

Entry nameZN143_HUMAN
AccessionPrimary (citable) accession number: P52747
Secondary accession number(s): A8K518 expand/collapse secondary AC list , B4DLY5, O75559, Q8WUK9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 5, 2009
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM