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P52747

- ZN143_HUMAN

UniProt

P52747 - ZN143_HUMAN

Protein

Zinc finger protein 143

Gene

ZNF143

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (05 May 2009)
      Previous versions | rss
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    Functioni

    Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri237 – 26125C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri267 – 29125C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri297 – 32125C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri327 – 35125C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri357 – 38125C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri387 – 41125C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri417 – 44024C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. gene expression Source: Reactome
    2. regulation of transcription from RNA polymerase III promoter Source: ProtInc
    3. regulation of transcription from RNA polymerase II promoter Source: ProtInc
    4. transcription from RNA polymerase III promoter Source: Reactome
    5. transcription from RNA polymerase II promoter Source: ProtInc

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
    REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein 143
    Alternative name(s):
    SPH-binding factor
    Selenocysteine tRNA gene transcription-activating factor
    Short name:
    hStaf
    Gene namesi
    Name:ZNF143
    Synonyms:SBF, STAF
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:12928. ZNF143.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37515.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 638638Zinc finger protein 143PRO_0000047426Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP52747.
    PaxDbiP52747.
    PRIDEiP52747.

    PTM databases

    PhosphoSiteiP52747.

    Expressioni

    Tissue specificityi

    Expressed in all tissues tested, with the strongest expression in ovary.

    Gene expression databases

    ArrayExpressiP52747.
    BgeeiP52747.
    CleanExiHS_ZNF143.
    GenevestigatoriP52747.

    Organism-specific databases

    HPAiHPA003263.

    Interactioni

    Subunit structurei

    Interacts with CHD8.1 Publication

    Protein-protein interaction databases

    BioGridi113496. 3 interactions.
    IntActiP52747. 1 interaction.
    STRINGi9606.ENSP00000379847.

    Structurei

    3D structure databases

    ProteinModelPortaliP52747.
    SMRiP52747. Positions 231-438.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri237 – 26125C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri267 – 29125C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri297 – 32125C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri327 – 35125C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri357 – 38125C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri387 – 41125C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri417 – 44024C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    HOGENOMiHOG000118073.
    HOVERGENiHBG053078.
    InParanoidiP52747.
    OMAiKMQIVLQ.
    OrthoDBiEOG7WX080.
    PhylomeDBiP52747.
    TreeFamiTF333498.

    Family and domain databases

    Gene3Di3.30.160.60. 7 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00096. zf-C2H2. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 7 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
    PS50157. ZINC_FINGER_C2H2_2. 7 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P52747-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL    50
    QAVTLADGST AYIQHNSKDA KLIDGQVIQL EDGSAAYVQH VPIPKSTGDS 100
    LRLEDGQAVQ LEDGTTAFIH HTSKDSYDQS ALQAVQLEDG TTAYIHHAVQ 150
    VPQSDTILAI QADGTVAGLH TGDATIDPDT ISALEQYAAK VSIDGSESVA 200
    GTGMIGENEQ EKKMQIVLQG HATRVTAKSQ QSGEKAFRCE YDGCGKLYTT 250
    AHHLKVHERS HTGDRPYQCE HAGCGKAFAT GYGLKSHVRT HTGEKPYRCS 300
    EDNCTKSFKT SGDLQKHIRT HTGERPFKCP FEGCGRSFTT SNIRKVHVRT 350
    HTGERPYYCT EPGCGRAFAS ATNYKNHVRI HTGEKPYVCT VPGCDKRFTE 400
    YSSLYKHHVV HTHSKPYNCN HCGKTYKQIS TLAMHKRTAH NDTEPIEEEQ 450
    EAFFEPPPGQ GEDVLKGSQI TYVTGVEGDD VVSTQVATVT QSGLSQQVTL 500
    ISQDGTQHVN ISQADMQAIG NTITMVTQDG TPITVPAHDA VISSAGTHSV 550
    AMVTAEGTEG EQVAIVAQDL AAFHTASSEM GHQQHSHHLV TTETRPLTLV 600
    ATSNGTQIAV QLGEQPSLEE AIRIASRIQQ GETPGLDD 638
    Length:638
    Mass (Da):68,896
    Last modified:May 5, 2009 - v2
    Checksum:iD149030016A6058A
    GO
    Isoform 2 (identifier: P52747-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         39-69: Missing.

    Show »
    Length:607
    Mass (Da):65,636
    Checksum:i285B599E182261D6
    GO
    Isoform 3 (identifier: P52747-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         97-98: TG → R

    Note: No experimental confirmation available.

    Show »
    Length:637
    Mass (Da):68,894
    Checksum:iF393F46093039A3C
    GO

    Sequence cautioni

    The sequence AAC50266.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH20219.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAF83822.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti433 – 4331A → V in AAH20219. (PubMed:15489334)Curated
    Sequence conflicti443 – 4431T → A in AK313330. (PubMed:14702039)Curated
    Sequence conflicti590 – 5901Missing in BAG59697. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti461 – 4611G → D.
    Corresponds to variant rs34972213 [ dbSNP | Ensembl ].
    VAR_061937
    Natural varianti561 – 5611E → Q.3 Publications
    Corresponds to variant rs10743108 [ dbSNP | Ensembl ].
    VAR_027254

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei39 – 6931Missing in isoform 2. 1 PublicationVSP_036978Add
    BLAST
    Alternative sequencei97 – 982TG → R in isoform 3. 1 PublicationVSP_055109

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK291133 mRNA. Translation: BAF83822.1. Different initiation.
    AK297214 mRNA. Translation: BAG59697.1.
    AK313330 mRNA. No translation available.
    AC127030 Genomic DNA. No translation available.
    AC132192 Genomic DNA. No translation available.
    BC020219 mRNA. Translation: AAH20219.1. Different initiation.
    U09850 mRNA. Translation: AAC50266.1. Different initiation.
    AF071771 mRNA. Translation: AAC96102.1.
    CCDSiCCDS60720.1. [P52747-3]
    CCDS60721.1. [P52747-2]
    CCDS7799.2. [P52747-1]
    PIRiI38618.
    RefSeqiNP_001269585.1. NM_001282656.1.
    NP_001269586.1. NM_001282657.1. [P52747-2]
    NP_003433.3. NM_003442.5. [P52747-1]
    UniGeneiHs.523471.

    Genome annotation databases

    EnsembliENST00000396597; ENSP00000379843; ENSG00000166478. [P52747-2]
    ENST00000396602; ENSP00000379847; ENSG00000166478. [P52747-1]
    ENST00000396604; ENSP00000379849; ENSG00000166478. [P52747-3]
    ENST00000530463; ENSP00000432154; ENSG00000166478. [P52747-3]
    GeneIDi7702.
    KEGGihsa:7702.
    UCSCiuc001mhr.3. human. [P52747-1]
    uc010rby.2. human. [P52747-2]

    Polymorphism databases

    DMDMi229462806.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK291133 mRNA. Translation: BAF83822.1 . Different initiation.
    AK297214 mRNA. Translation: BAG59697.1 .
    AK313330 mRNA. No translation available.
    AC127030 Genomic DNA. No translation available.
    AC132192 Genomic DNA. No translation available.
    BC020219 mRNA. Translation: AAH20219.1 . Different initiation.
    U09850 mRNA. Translation: AAC50266.1 . Different initiation.
    AF071771 mRNA. Translation: AAC96102.1 .
    CCDSi CCDS60720.1. [P52747-3 ]
    CCDS60721.1. [P52747-2 ]
    CCDS7799.2. [P52747-1 ]
    PIRi I38618.
    RefSeqi NP_001269585.1. NM_001282656.1.
    NP_001269586.1. NM_001282657.1. [P52747-2 ]
    NP_003433.3. NM_003442.5. [P52747-1 ]
    UniGenei Hs.523471.

    3D structure databases

    ProteinModelPortali P52747.
    SMRi P52747. Positions 231-438.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113496. 3 interactions.
    IntActi P52747. 1 interaction.
    STRINGi 9606.ENSP00000379847.

    PTM databases

    PhosphoSitei P52747.

    Polymorphism databases

    DMDMi 229462806.

    Proteomic databases

    MaxQBi P52747.
    PaxDbi P52747.
    PRIDEi P52747.

    Protocols and materials databases

    DNASUi 7702.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000396597 ; ENSP00000379843 ; ENSG00000166478 . [P52747-2 ]
    ENST00000396602 ; ENSP00000379847 ; ENSG00000166478 . [P52747-1 ]
    ENST00000396604 ; ENSP00000379849 ; ENSG00000166478 . [P52747-3 ]
    ENST00000530463 ; ENSP00000432154 ; ENSG00000166478 . [P52747-3 ]
    GeneIDi 7702.
    KEGGi hsa:7702.
    UCSCi uc001mhr.3. human. [P52747-1 ]
    uc010rby.2. human. [P52747-2 ]

    Organism-specific databases

    CTDi 7702.
    GeneCardsi GC11P009483.
    HGNCi HGNC:12928. ZNF143.
    HPAi HPA003263.
    MIMi 603433. gene.
    neXtProti NX_P52747.
    PharmGKBi PA37515.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5048.
    HOGENOMi HOG000118073.
    HOVERGENi HBG053078.
    InParanoidi P52747.
    OMAi KMQIVLQ.
    OrthoDBi EOG7WX080.
    PhylomeDBi P52747.
    TreeFami TF333498.

    Enzyme and pathway databases

    Reactomei REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
    REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

    Miscellaneous databases

    ChiTaRSi ZNF143. human.
    GeneWikii ZNF143.
    GenomeRNAii 7702.
    NextBioi 29854.
    PROi P52747.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P52747.
    Bgeei P52747.
    CleanExi HS_ZNF143.
    Genevestigatori P52747.

    Family and domain databases

    Gene3Di 3.30.160.60. 7 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00096. zf-C2H2. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 7 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 7 hits.
    PS50157. ZINC_FINGER_C2H2_2. 7 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3), VARIANT GLN-561.
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLN-561.
      Tissue: Muscle.
    4. "Isolation and fine mapping of 16 novel human zinc finger-encoding cDNAs identify putative candidate genes for developmental and malignant disorders."
      Tommerup N., Vissing H.
      Genomics 27:259-264(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-638 (ISOFORM 1).
      Tissue: Insulinoma.
    5. "Molecular cloning of a cDNA encoding human SPH-binding factor, a conserved protein that binds to the enhancer-like region of the U6 small nuclear RNA gene promoter."
      Rincon J.C., Engler S.K., Hargrove B.W., Kunkel G.R.
      Nucleic Acids Res. 26:4846-4852(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 88-638 (ISOFORMS 1/2), VARIANT GLN-561, FUNCTION.
    6. "CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription."
      Yuan C.-C., Zhao X., Florens L., Swanson S.K., Washburn M.P., Hernandez N.
      Mol. Cell. Biol. 27:8729-8738(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CHD8.
    7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiZN143_HUMAN
    AccessioniPrimary (citable) accession number: P52747
    Secondary accession number(s): A8K518
    , B4DLY5, E7ER34, O75559, Q8WUK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3