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Protein

FYVE, RhoGEF and PH domain-containing protein 1

Gene

Fgd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri729 – 78961FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. guanyl-nucleotide exchange factor activity Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. Rho guanyl-nucleotide exchange factor activity Source: InterPro
  4. small GTPase binding Source: MGI

GO - Biological processi

  1. actin cytoskeleton organization Source: MGI
  2. cytoskeleton organization Source: MGI
  3. filopodium assembly Source: MGI
  4. positive regulation of GTPase activity Source: MGI
  5. regulation of cell shape Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_289095. NRAGE signals death through JNK.
REACT_297947. Rho GTPase cycle.
REACT_299052. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
FYVE, RhoGEF and PH domain-containing protein 1
Alternative name(s):
Faciogenital dysplasia 1 protein homolog
Rho/Rac guanine nucleotide exchange factor FGD1
Short name:
Rho/Rac GEF
Zinc finger FYVE domain-containing protein 3
Gene namesi
Name:Fgd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:104566. Fgd1.

Subcellular locationi

  1. Cytoplasm 1 Publication
  2. Cell projectionlamellipodium 1 Publication
  3. Cell projectionruffle 1 Publication
  4. Cytoplasmcytoskeleton 1 Publication

  5. Note: Associated with membrane ruffles and lamellipodia.

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytoskeleton Source: UniProtKB-SubCell
  3. Golgi apparatus Source: MGI
  4. lamellipodium Source: MGI
  5. ruffle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi159 – 1591P → A: Abolishes binding to DBNL. 1 Publication
Mutagenesisi162 – 1621P → A: Abolishes binding to DBNL. 1 Publication
Mutagenesisi164 – 1641K → E: No effect on binding to DBNL. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 960960FYVE, RhoGEF and PH domain-containing protein 1PRO_0000080941Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481PhosphoserineBy similarity
Modified residuei205 – 2051PhosphoserineBy similarity
Modified residuei710 – 7101PhosphothreonineBy similarity
Modified residuei714 – 7141PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP52734.
PaxDbiP52734.
PRIDEiP52734.

PTM databases

PhosphoSiteiP52734.

Expressioni

Gene expression databases

BgeeiP52734.
CleanExiMM_FGD1.
ExpressionAtlasiP52734. baseline and differential.
GenevestigatoriP52734.

Interactioni

Subunit structurei

Interacts with DBNL/ABP1 and CTTN. Binds CDC42 (By similarity). May interact with CCPG1.By similarity1 Publication

Protein-protein interaction databases

BioGridi199637. 2 interactions.
IntActiP52734. 2 interactions.
MINTiMINT-272222.

Structurei

3D structure databases

ProteinModelPortaliP52734.
SMRiP52734. Positions 364-791, 830-919.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini372 – 560189DHPROSITE-ProRule annotationAdd
BLAST
Domaini589 – 688100PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini820 – 920101PH 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi171 – 18717SH3-bindingSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 330324Pro-richAdd
BLAST

Domaini

The DH domain is involved in interaction with CCPG1.

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri729 – 78961FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG295069.
GeneTreeiENSGT00750000117280.
HOGENOMiHOG000220866.
HOVERGENiHBG007506.
InParanoidiP52734.
KOiK05720.
OMAiLQMPRMP.
TreeFamiTF316247.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000219. DH-domain.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF00169. PH. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHGHRVPGGP GPSDPERSAA NTPGAAPLAC ADSDPGALEP GLPVSRGSGT
60 70 80 90 100
ALGGPLDPQF VGPSDASLGA PPSSRVLPCG PSPQHHRALR FSYHLEGSQP
110 120 130 140 150
RPGLHQGNRI LVKSLSLDPG QSLEPHPEGP QRLRSDPGPP TEIPGPRPSP
160 170 180 190 200
LKRAPGPKPQ VPPKPSYLQM PRVLPPPEPI PPPPSRPLPA DPRVAKGLVP
210 220 230 240 250
RAEASTSSAA VSSLIEKFER EPVIVASDRP APGPCPVPPE PAMLPQPPPQ
260 270 280 290 300
PTGSQLPEGE ASRCLFLLAP GPRDGEKVPN RDSGIDSISS PSNSEETCFV
310 320 330 340 350
SDDGPPIHSL CPGPPALASM PVALADPHRP GSQEVDSDLE EEEEEEEEEK
360 370 380 390 400
EREIPVPPME RQESVELTVQ QKVFHIANEL LQTEKAYVSR LHLLDQVFCA
410 420 430 440 450
RLLEEARNRS SFPADVVHGI FSNICSIYCF HQQFLLPELE KRMEEWDRYP
460 470 480 490 500
RIGDILQKLA PFLKMYGEYV KNFDRAVELV NTWTERSTQF KVIIHEVQKE
510 520 530 540 550
EACGNLTLQH HMLEPVQRIP RYELLLKDYL LKLPHGSPDS KDAQKSLELI
560 570 580 590 600
ATAAEHSNAA IRKMERMHKL LKVYELLGGE EDIVSPTKEL IKEGHILKLS
610 620 630 640 650
AKNGTTQDRY LILFNDRLLY CVPRLRLLGQ KFSVRARIDV DGMELKESSN
660 670 680 690 700
LNMPRTFLVS GKQRSLELQA RTEEEKKDWV QAINSTLLKH EQTLETFKLL
710 720 730 740 750
NSTNRDDEDT PPNSPNVDLG KRAPTPIREK EVTMCMRCQE PFNSITKRRH
760 770 780 790 800
HCKACGHVVC GKCSEFRARL IYDNNRSNRV CTDCYVALHG APGSSPACSQ
810 820 830 840 850
HTPQRRRSIL EKQASVAAEN SVICSFLHYM EKGGKGWHKA WFVVPENEPL
860 870 880 890 900
VLYIYGAPQD VKAQRSLPLI GFEVGPPEAG ERPDRRHVFK ITQSHLSWYF
910 920 930 940 950
SPETEELQRR WMAVLGRAGR GDTFCPGPTL SEDKEMEETP VAASGATAEP
960
PEASQTRDKT
Length:960
Mass (Da):106,365
Last modified:July 27, 2011 - v2
Checksum:iE3E489BA2F3AE056
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti504 – 5041G → R in AAA96001 (PubMed:8535076).Curated
Sequence conflicti633 – 6331S → T in AAA96001 (PubMed:8535076).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22325 mRNA. Translation: AAA96001.1.
AL805937 Genomic DNA. Translation: CAM14870.1.
BC011462 mRNA. Translation: AAH11462.1.
CCDSiCCDS30467.1.
RefSeqiNP_032027.2. NM_008001.4.
UniGeneiMm.475704.
Mm.8142.

Genome annotation databases

EnsembliENSMUST00000026296; ENSMUSP00000026296; ENSMUSG00000025265.
GeneIDi14163.
KEGGimmu:14163.
UCSCiuc009uow.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22325 mRNA. Translation: AAA96001.1.
AL805937 Genomic DNA. Translation: CAM14870.1.
BC011462 mRNA. Translation: AAH11462.1.
CCDSiCCDS30467.1.
RefSeqiNP_032027.2. NM_008001.4.
UniGeneiMm.475704.
Mm.8142.

3D structure databases

ProteinModelPortaliP52734.
SMRiP52734. Positions 364-791, 830-919.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199637. 2 interactions.
IntActiP52734. 2 interactions.
MINTiMINT-272222.

PTM databases

PhosphoSiteiP52734.

Proteomic databases

MaxQBiP52734.
PaxDbiP52734.
PRIDEiP52734.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026296; ENSMUSP00000026296; ENSMUSG00000025265.
GeneIDi14163.
KEGGimmu:14163.
UCSCiuc009uow.1. mouse.

Organism-specific databases

CTDi2245.
MGIiMGI:104566. Fgd1.

Phylogenomic databases

eggNOGiNOG295069.
GeneTreeiENSGT00750000117280.
HOGENOMiHOG000220866.
HOVERGENiHBG007506.
InParanoidiP52734.
KOiK05720.
OMAiLQMPRMP.
TreeFamiTF316247.

Enzyme and pathway databases

ReactomeiREACT_289095. NRAGE signals death through JNK.
REACT_297947. Rho GTPase cycle.
REACT_299052. G alpha (12/13) signalling events.

Miscellaneous databases

ChiTaRSiFgd1. mouse.
NextBioi285298.
PROiP52734.
SOURCEiSearch...

Gene expression databases

BgeeiP52734.
CleanExiMM_FGD1.
ExpressionAtlasiP52734. baseline and differential.
GenevestigatoriP52734.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000219. DH-domain.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF00169. PH. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and regional localization of the mouse faciogenital dysplasia (Fgd1) gene."
    Pasteris N.G., de Gouyon B., Cadle A.B., Campbell K., Herman G.E., Gorski J.L.
    Mamm. Genome 6:658-661(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Fgd1, the Cdc42 GEF responsible for faciogenital dysplasia, directly interacts with cortactin and mAbp1 to modulate cell shape."
    Hou P., Estrada L., Kinley A.W., Parsons J.T., Vojtek A.B., Gorski J.L.
    Hum. Mol. Genet. 12:1981-1993(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DBNL AND CTTN, MUTAGENESIS OF PRO-159; PRO-162 AND LYS-164.
  5. "Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs."
    Kostenko E.V., Olabisi O.O., Sahay S., Rodriguez P.L., Whitehead I.P.
    Mol. Cell. Biol. 26:8964-8975(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE INTERACTION WITH CCPG1.

Entry informationi

Entry nameiFGD1_MOUSE
AccessioniPrimary (citable) accession number: P52734
Secondary accession number(s): Q921L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: April 29, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.