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P52734

- FGD1_MOUSE

UniProt

P52734 - FGD1_MOUSE

Protein

FYVE, RhoGEF and PH domain-containing protein 1

Gene

Fgd1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri729 – 78961FYVE-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: UniProtKB
    3. Rho guanyl-nucleotide exchange factor activity Source: InterPro

    GO - Biological processi

    1. actin cytoskeleton organization Source: Ensembl
    2. cytoskeleton organization Source: MGI
    3. filopodium assembly Source: Ensembl
    4. regulation of Cdc42 GTPase activity Source: Ensembl
    5. regulation of cell shape Source: UniProtKB

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_204733. G alpha (12/13) signalling events.
    REACT_209641. NRAGE signals death through JNK.
    REACT_210090. Rho GTPase cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    FYVE, RhoGEF and PH domain-containing protein 1
    Alternative name(s):
    Faciogenital dysplasia 1 protein homolog
    Rho/Rac guanine nucleotide exchange factor FGD1
    Short name:
    Rho/Rac GEF
    Zinc finger FYVE domain-containing protein 3
    Gene namesi
    Name:Fgd1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:104566. Fgd1.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell projectionlamellipodium 1 Publication. Cell projectionruffle 1 Publication. Cytoplasmcytoskeleton 1 Publication
    Note: Associated with membrane ruffles and lamellipodia.

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. cytoskeleton Source: UniProtKB-SubCell
    3. Golgi apparatus Source: MGI
    4. lamellipodium Source: MGI
    5. nucleus Source: Ensembl
    6. plasma membrane Source: Ensembl
    7. ruffle Source: MGI

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi159 – 1591P → A: Abolishes binding to DBNL. 1 Publication
    Mutagenesisi162 – 1621P → A: Abolishes binding to DBNL. 1 Publication
    Mutagenesisi164 – 1641K → E: No effect on binding to DBNL. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 960960FYVE, RhoGEF and PH domain-containing protein 1PRO_0000080941Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei48 – 481PhosphoserineBy similarity
    Modified residuei205 – 2051PhosphoserineBy similarity
    Modified residuei364 – 3641PhosphoserineBy similarity
    Modified residuei710 – 7101PhosphothreonineBy similarity
    Modified residuei714 – 7141PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP52734.
    PaxDbiP52734.
    PRIDEiP52734.

    PTM databases

    PhosphoSiteiP52734.

    Expressioni

    Gene expression databases

    BgeeiP52734.
    CleanExiMM_FGD1.
    GenevestigatoriP52734.

    Interactioni

    Subunit structurei

    Interacts with DBNL/ABP1 and CTTN. Binds CDC42 By similarity. May interact with CCPG1.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi199637. 2 interactions.
    IntActiP52734. 2 interactions.
    MINTiMINT-272222.

    Structurei

    3D structure databases

    ProteinModelPortaliP52734.
    SMRiP52734. Positions 364-791, 830-919.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini372 – 560189DHPROSITE-ProRule annotationAdd
    BLAST
    Domaini589 – 688100PH 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini820 – 920101PH 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi171 – 18717SH3-bindingSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi7 – 330324Pro-richAdd
    BLAST

    Domaini

    The DH domain is involved in interaction with CCPG1.

    Sequence similaritiesi

    Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
    Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
    Contains 2 PH domains.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri729 – 78961FYVE-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG295069.
    GeneTreeiENSGT00750000117280.
    HOGENOMiHOG000220866.
    HOVERGENiHBG007506.
    InParanoidiQ921L2.
    KOiK05720.
    OMAiLQMPRMP.
    TreeFamiTF316247.

    Family and domain databases

    Gene3Di1.20.900.10. 1 hit.
    2.30.29.30. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR000219. DH-domain.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000306. Znf_FYVE.
    IPR017455. Znf_FYVE-rel.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF01363. FYVE. 1 hit.
    PF00169. PH. 2 hits.
    PF00621. RhoGEF. 1 hit.
    [Graphical view]
    SMARTiSM00064. FYVE. 1 hit.
    SM00233. PH. 2 hits.
    SM00325. RhoGEF. 1 hit.
    [Graphical view]
    SUPFAMiSSF48065. SSF48065. 1 hit.
    PROSITEiPS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 2 hits.
    PS50178. ZF_FYVE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52734-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHGHRVPGGP GPSDPERSAA NTPGAAPLAC ADSDPGALEP GLPVSRGSGT    50
    ALGGPLDPQF VGPSDASLGA PPSSRVLPCG PSPQHHRALR FSYHLEGSQP 100
    RPGLHQGNRI LVKSLSLDPG QSLEPHPEGP QRLRSDPGPP TEIPGPRPSP 150
    LKRAPGPKPQ VPPKPSYLQM PRVLPPPEPI PPPPSRPLPA DPRVAKGLVP 200
    RAEASTSSAA VSSLIEKFER EPVIVASDRP APGPCPVPPE PAMLPQPPPQ 250
    PTGSQLPEGE ASRCLFLLAP GPRDGEKVPN RDSGIDSISS PSNSEETCFV 300
    SDDGPPIHSL CPGPPALASM PVALADPHRP GSQEVDSDLE EEEEEEEEEK 350
    EREIPVPPME RQESVELTVQ QKVFHIANEL LQTEKAYVSR LHLLDQVFCA 400
    RLLEEARNRS SFPADVVHGI FSNICSIYCF HQQFLLPELE KRMEEWDRYP 450
    RIGDILQKLA PFLKMYGEYV KNFDRAVELV NTWTERSTQF KVIIHEVQKE 500
    EACGNLTLQH HMLEPVQRIP RYELLLKDYL LKLPHGSPDS KDAQKSLELI 550
    ATAAEHSNAA IRKMERMHKL LKVYELLGGE EDIVSPTKEL IKEGHILKLS 600
    AKNGTTQDRY LILFNDRLLY CVPRLRLLGQ KFSVRARIDV DGMELKESSN 650
    LNMPRTFLVS GKQRSLELQA RTEEEKKDWV QAINSTLLKH EQTLETFKLL 700
    NSTNRDDEDT PPNSPNVDLG KRAPTPIREK EVTMCMRCQE PFNSITKRRH 750
    HCKACGHVVC GKCSEFRARL IYDNNRSNRV CTDCYVALHG APGSSPACSQ 800
    HTPQRRRSIL EKQASVAAEN SVICSFLHYM EKGGKGWHKA WFVVPENEPL 850
    VLYIYGAPQD VKAQRSLPLI GFEVGPPEAG ERPDRRHVFK ITQSHLSWYF 900
    SPETEELQRR WMAVLGRAGR GDTFCPGPTL SEDKEMEETP VAASGATAEP 950
    PEASQTRDKT 960
    Length:960
    Mass (Da):106,365
    Last modified:July 27, 2011 - v2
    Checksum:iE3E489BA2F3AE056
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti504 – 5041G → R in AAA96001. (PubMed:8535076)Curated
    Sequence conflicti633 – 6331S → T in AAA96001. (PubMed:8535076)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22325 mRNA. Translation: AAA96001.1.
    AL805937 Genomic DNA. Translation: CAM14870.1.
    BC011462 mRNA. Translation: AAH11462.1.
    CCDSiCCDS30467.1.
    RefSeqiNP_032027.2. NM_008001.4.
    UniGeneiMm.475704.
    Mm.8142.

    Genome annotation databases

    EnsembliENSMUST00000026296; ENSMUSP00000026296; ENSMUSG00000025265.
    GeneIDi14163.
    KEGGimmu:14163.
    UCSCiuc009uow.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22325 mRNA. Translation: AAA96001.1 .
    AL805937 Genomic DNA. Translation: CAM14870.1 .
    BC011462 mRNA. Translation: AAH11462.1 .
    CCDSi CCDS30467.1.
    RefSeqi NP_032027.2. NM_008001.4.
    UniGenei Mm.475704.
    Mm.8142.

    3D structure databases

    ProteinModelPortali P52734.
    SMRi P52734. Positions 364-791, 830-919.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199637. 2 interactions.
    IntActi P52734. 2 interactions.
    MINTi MINT-272222.

    PTM databases

    PhosphoSitei P52734.

    Proteomic databases

    MaxQBi P52734.
    PaxDbi P52734.
    PRIDEi P52734.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026296 ; ENSMUSP00000026296 ; ENSMUSG00000025265 .
    GeneIDi 14163.
    KEGGi mmu:14163.
    UCSCi uc009uow.1. mouse.

    Organism-specific databases

    CTDi 2245.
    MGIi MGI:104566. Fgd1.

    Phylogenomic databases

    eggNOGi NOG295069.
    GeneTreei ENSGT00750000117280.
    HOGENOMi HOG000220866.
    HOVERGENi HBG007506.
    InParanoidi Q921L2.
    KOi K05720.
    OMAi LQMPRMP.
    TreeFami TF316247.

    Enzyme and pathway databases

    Reactomei REACT_204733. G alpha (12/13) signalling events.
    REACT_209641. NRAGE signals death through JNK.
    REACT_210090. Rho GTPase cycle.

    Miscellaneous databases

    ChiTaRSi FGD1. mouse.
    NextBioi 285298.
    PROi P52734.
    SOURCEi Search...

    Gene expression databases

    Bgeei P52734.
    CleanExi MM_FGD1.
    Genevestigatori P52734.

    Family and domain databases

    Gene3Di 1.20.900.10. 1 hit.
    2.30.29.30. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR000219. DH-domain.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000306. Znf_FYVE.
    IPR017455. Znf_FYVE-rel.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF01363. FYVE. 1 hit.
    PF00169. PH. 2 hits.
    PF00621. RhoGEF. 1 hit.
    [Graphical view ]
    SMARTi SM00064. FYVE. 1 hit.
    SM00233. PH. 2 hits.
    SM00325. RhoGEF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48065. SSF48065. 1 hit.
    PROSITEi PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 2 hits.
    PS50178. ZF_FYVE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and regional localization of the mouse faciogenital dysplasia (Fgd1) gene."
      Pasteris N.G., de Gouyon B., Cadle A.B., Campbell K., Herman G.E., Gorski J.L.
      Mamm. Genome 6:658-661(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    4. "Fgd1, the Cdc42 GEF responsible for faciogenital dysplasia, directly interacts with cortactin and mAbp1 to modulate cell shape."
      Hou P., Estrada L., Kinley A.W., Parsons J.T., Vojtek A.B., Gorski J.L.
      Hum. Mol. Genet. 12:1981-1993(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DBNL AND CTTN, MUTAGENESIS OF PRO-159; PRO-162 AND LYS-164.
    5. "Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs."
      Kostenko E.V., Olabisi O.O., Sahay S., Rodriguez P.L., Whitehead I.P.
      Mol. Cell. Biol. 26:8964-8975(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE INTERACTION WITH CCPG1.

    Entry informationi

    Entry nameiFGD1_MOUSE
    AccessioniPrimary (citable) accession number: P52734
    Secondary accession number(s): Q921L2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3