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P52734 (FGD1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
FYVE, RhoGEF and PH domain-containing protein 1
Alternative name(s):
Faciogenital dysplasia 1 protein homolog
Rho/Rac guanine nucleotide exchange factor FGD1
Short name=Rho/Rac GEF
Zinc finger FYVE domain-containing protein 3
Gene names
Name:Fgd1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length960 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Ref.4

Subunit structure

Interacts with DBNL/ABP1 and CTTN. Binds CDC42 By similarity. May interact with CCPG1. Ref.4 Ref.5

Subcellular location

Cytoplasm. Cell projectionlamellipodium. Cell projectionruffle. Cytoplasmcytoskeleton. Note: Associated with membrane ruffles and lamellipodia. Ref.4

Domain

The DH domain is involved in interaction with CCPG1.

Sequence similarities

Contains 1 DH (DBL-homology) domain.

Contains 1 FYVE-type zinc finger.

Contains 2 PH domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 960960FYVE, RhoGEF and PH domain-containing protein 1
PRO_0000080941

Regions

Domain372 – 560189DH
Domain589 – 688100PH 1
Domain820 – 920101PH 2
Zinc finger729 – 78961FYVE-type
Motif171 – 18717SH3-binding Potential
Compositional bias7 – 330324Pro-rich

Amino acid modifications

Modified residue481Phosphoserine By similarity
Modified residue2051Phosphoserine By similarity
Modified residue3641Phosphoserine By similarity
Modified residue7101Phosphothreonine By similarity
Modified residue7141Phosphoserine By similarity

Experimental info

Mutagenesis1591P → A: Abolishes binding to DBNL. Ref.4
Mutagenesis1621P → A: Abolishes binding to DBNL. Ref.4
Mutagenesis1641K → E: No effect on binding to DBNL. Ref.4
Sequence conflict5041G → R in AAA96001. Ref.1
Sequence conflict6331S → T in AAA96001. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P52734 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: E3E489BA2F3AE056

FASTA960106,365
        10         20         30         40         50         60 
MHGHRVPGGP GPSDPERSAA NTPGAAPLAC ADSDPGALEP GLPVSRGSGT ALGGPLDPQF 

        70         80         90        100        110        120 
VGPSDASLGA PPSSRVLPCG PSPQHHRALR FSYHLEGSQP RPGLHQGNRI LVKSLSLDPG 

       130        140        150        160        170        180 
QSLEPHPEGP QRLRSDPGPP TEIPGPRPSP LKRAPGPKPQ VPPKPSYLQM PRVLPPPEPI 

       190        200        210        220        230        240 
PPPPSRPLPA DPRVAKGLVP RAEASTSSAA VSSLIEKFER EPVIVASDRP APGPCPVPPE 

       250        260        270        280        290        300 
PAMLPQPPPQ PTGSQLPEGE ASRCLFLLAP GPRDGEKVPN RDSGIDSISS PSNSEETCFV 

       310        320        330        340        350        360 
SDDGPPIHSL CPGPPALASM PVALADPHRP GSQEVDSDLE EEEEEEEEEK EREIPVPPME 

       370        380        390        400        410        420 
RQESVELTVQ QKVFHIANEL LQTEKAYVSR LHLLDQVFCA RLLEEARNRS SFPADVVHGI 

       430        440        450        460        470        480 
FSNICSIYCF HQQFLLPELE KRMEEWDRYP RIGDILQKLA PFLKMYGEYV KNFDRAVELV 

       490        500        510        520        530        540 
NTWTERSTQF KVIIHEVQKE EACGNLTLQH HMLEPVQRIP RYELLLKDYL LKLPHGSPDS 

       550        560        570        580        590        600 
KDAQKSLELI ATAAEHSNAA IRKMERMHKL LKVYELLGGE EDIVSPTKEL IKEGHILKLS 

       610        620        630        640        650        660 
AKNGTTQDRY LILFNDRLLY CVPRLRLLGQ KFSVRARIDV DGMELKESSN LNMPRTFLVS 

       670        680        690        700        710        720 
GKQRSLELQA RTEEEKKDWV QAINSTLLKH EQTLETFKLL NSTNRDDEDT PPNSPNVDLG 

       730        740        750        760        770        780 
KRAPTPIREK EVTMCMRCQE PFNSITKRRH HCKACGHVVC GKCSEFRARL IYDNNRSNRV 

       790        800        810        820        830        840 
CTDCYVALHG APGSSPACSQ HTPQRRRSIL EKQASVAAEN SVICSFLHYM EKGGKGWHKA 

       850        860        870        880        890        900 
WFVVPENEPL VLYIYGAPQD VKAQRSLPLI GFEVGPPEAG ERPDRRHVFK ITQSHLSWYF 

       910        920        930        940        950        960 
SPETEELQRR WMAVLGRAGR GDTFCPGPTL SEDKEMEETP VAASGATAEP PEASQTRDKT 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and regional localization of the mouse faciogenital dysplasia (Fgd1) gene."
Pasteris N.G., de Gouyon B., Cadle A.B., Campbell K., Herman G.E., Gorski J.L.
Mamm. Genome 6:658-661(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"Fgd1, the Cdc42 GEF responsible for faciogenital dysplasia, directly interacts with cortactin and mAbp1 to modulate cell shape."
Hou P., Estrada L., Kinley A.W., Parsons J.T., Vojtek A.B., Gorski J.L.
Hum. Mol. Genet. 12:1981-1993(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DBNL AND CTTN, MUTAGENESIS OF PRO-159; PRO-162 AND LYS-164.
[5]"Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs."
Kostenko E.V., Olabisi O.O., Sahay S., Rodriguez P.L., Whitehead I.P.
Mol. Cell. Biol. 26:8964-8975(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: POSSIBLE INTERACTION WITH CCPG1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U22325 mRNA. Translation: AAA96001.1.
AL805937 Genomic DNA. Translation: CAM14870.1.
BC011462 mRNA. Translation: AAH11462.1.
CCDSCCDS30467.1.
RefSeqNP_032027.2. NM_008001.4.
UniGeneMm.475704.
Mm.8142.

3D structure databases

ProteinModelPortalP52734.
SMRP52734. Positions 364-791, 830-919.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199637. 2 interactions.
IntActP52734. 2 interactions.
MINTMINT-272222.

PTM databases

PhosphoSiteP52734.

Proteomic databases

MaxQBP52734.
PaxDbP52734.
PRIDEP52734.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026296; ENSMUSP00000026296; ENSMUSG00000025265.
GeneID14163.
KEGGmmu:14163.
UCSCuc009uow.1. mouse.

Organism-specific databases

CTD2245.
MGIMGI:104566. Fgd1.

Phylogenomic databases

eggNOGNOG295069.
GeneTreeENSGT00750000117280.
HOGENOMHOG000220866.
HOVERGENHBG007506.
InParanoidQ921L2.
KOK05720.
OMALQMPRMP.
TreeFamTF316247.

Gene expression databases

BgeeP52734.
CleanExMM_FGD1.
GenevestigatorP52734.

Family and domain databases

Gene3D1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
3.30.40.10. 1 hit.
InterProIPR000219. DH-domain.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF01363. FYVE. 1 hit.
PF00169. PH. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTSM00064. FYVE. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMSSF48065. SSF48065. 1 hit.
PROSITEPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFGD1. mouse.
NextBio285298.
PROP52734.
SOURCESearch...

Entry information

Entry nameFGD1_MOUSE
AccessionPrimary (citable) accession number: P52734
Secondary accession number(s): Q921L2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot