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P52734

- FGD1_MOUSE

UniProt

P52734 - FGD1_MOUSE

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Protein
FYVE, RhoGEF and PH domain-containing protein 1
Gene
Fgd1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri729 – 78961FYVE-type
Add
BLAST

GO - Molecular functioni

  1. Rho guanyl-nucleotide exchange factor activity Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: UniProtKB

GO - Biological processi

  1. actin cytoskeleton organization Source: Ensembl
  2. cytoskeleton organization Source: MGI
  3. filopodium assembly Source: Ensembl
  4. regulation of Cdc42 GTPase activity Source: Ensembl
  5. regulation of cell shape Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_204733. G alpha (12/13) signalling events.
REACT_209641. NRAGE signals death through JNK.
REACT_210090. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
FYVE, RhoGEF and PH domain-containing protein 1
Alternative name(s):
Faciogenital dysplasia 1 protein homolog
Rho/Rac guanine nucleotide exchange factor FGD1
Short name:
Rho/Rac GEF
Zinc finger FYVE domain-containing protein 3
Gene namesi
Name:Fgd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:104566. Fgd1.

Subcellular locationi

Cytoplasm. Cell projectionlamellipodium. Cell projectionruffle. Cytoplasmcytoskeleton
Note: Associated with membrane ruffles and lamellipodia.1 Publication

GO - Cellular componenti

  1. Golgi apparatus Source: MGI
  2. cytoplasm Source: MGI
  3. cytoskeleton Source: UniProtKB-SubCell
  4. lamellipodium Source: MGI
  5. nucleus Source: Ensembl
  6. plasma membrane Source: Ensembl
  7. ruffle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi159 – 1591P → A: Abolishes binding to DBNL. 1 Publication
Mutagenesisi162 – 1621P → A: Abolishes binding to DBNL. 1 Publication
Mutagenesisi164 – 1641K → E: No effect on binding to DBNL. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 960960FYVE, RhoGEF and PH domain-containing protein 1
PRO_0000080941Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481Phosphoserine By similarity
Modified residuei205 – 2051Phosphoserine By similarity
Modified residuei364 – 3641Phosphoserine By similarity
Modified residuei710 – 7101Phosphothreonine By similarity
Modified residuei714 – 7141Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP52734.
PaxDbiP52734.
PRIDEiP52734.

PTM databases

PhosphoSiteiP52734.

Expressioni

Gene expression databases

BgeeiP52734.
CleanExiMM_FGD1.
GenevestigatoriP52734.

Interactioni

Subunit structurei

Interacts with DBNL/ABP1 and CTTN. Binds CDC42 By similarity. May interact with CCPG1.2 Publications

Protein-protein interaction databases

BioGridi199637. 2 interactions.
IntActiP52734. 2 interactions.
MINTiMINT-272222.

Structurei

3D structure databases

ProteinModelPortaliP52734.
SMRiP52734. Positions 364-791, 830-919.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini372 – 560189DH
Add
BLAST
Domaini589 – 688100PH 1
Add
BLAST
Domaini820 – 920101PH 2
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi171 – 18717SH3-binding Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 330324Pro-rich
Add
BLAST

Domaini

The DH domain is involved in interaction with CCPG1.

Sequence similaritiesi

Contains 2 PH domains.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG295069.
GeneTreeiENSGT00750000117280.
HOGENOMiHOG000220866.
HOVERGENiHBG007506.
InParanoidiQ921L2.
KOiK05720.
OMAiLQMPRMP.
TreeFamiTF316247.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000219. DH-domain.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF00169. PH. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52734-1 [UniParc]FASTAAdd to Basket

« Hide

MHGHRVPGGP GPSDPERSAA NTPGAAPLAC ADSDPGALEP GLPVSRGSGT    50
ALGGPLDPQF VGPSDASLGA PPSSRVLPCG PSPQHHRALR FSYHLEGSQP 100
RPGLHQGNRI LVKSLSLDPG QSLEPHPEGP QRLRSDPGPP TEIPGPRPSP 150
LKRAPGPKPQ VPPKPSYLQM PRVLPPPEPI PPPPSRPLPA DPRVAKGLVP 200
RAEASTSSAA VSSLIEKFER EPVIVASDRP APGPCPVPPE PAMLPQPPPQ 250
PTGSQLPEGE ASRCLFLLAP GPRDGEKVPN RDSGIDSISS PSNSEETCFV 300
SDDGPPIHSL CPGPPALASM PVALADPHRP GSQEVDSDLE EEEEEEEEEK 350
EREIPVPPME RQESVELTVQ QKVFHIANEL LQTEKAYVSR LHLLDQVFCA 400
RLLEEARNRS SFPADVVHGI FSNICSIYCF HQQFLLPELE KRMEEWDRYP 450
RIGDILQKLA PFLKMYGEYV KNFDRAVELV NTWTERSTQF KVIIHEVQKE 500
EACGNLTLQH HMLEPVQRIP RYELLLKDYL LKLPHGSPDS KDAQKSLELI 550
ATAAEHSNAA IRKMERMHKL LKVYELLGGE EDIVSPTKEL IKEGHILKLS 600
AKNGTTQDRY LILFNDRLLY CVPRLRLLGQ KFSVRARIDV DGMELKESSN 650
LNMPRTFLVS GKQRSLELQA RTEEEKKDWV QAINSTLLKH EQTLETFKLL 700
NSTNRDDEDT PPNSPNVDLG KRAPTPIREK EVTMCMRCQE PFNSITKRRH 750
HCKACGHVVC GKCSEFRARL IYDNNRSNRV CTDCYVALHG APGSSPACSQ 800
HTPQRRRSIL EKQASVAAEN SVICSFLHYM EKGGKGWHKA WFVVPENEPL 850
VLYIYGAPQD VKAQRSLPLI GFEVGPPEAG ERPDRRHVFK ITQSHLSWYF 900
SPETEELQRR WMAVLGRAGR GDTFCPGPTL SEDKEMEETP VAASGATAEP 950
PEASQTRDKT 960
Length:960
Mass (Da):106,365
Last modified:July 27, 2011 - v2
Checksum:iE3E489BA2F3AE056
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti504 – 5041G → R in AAA96001. 1 Publication
Sequence conflicti633 – 6331S → T in AAA96001. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22325 mRNA. Translation: AAA96001.1.
AL805937 Genomic DNA. Translation: CAM14870.1.
BC011462 mRNA. Translation: AAH11462.1.
CCDSiCCDS30467.1.
RefSeqiNP_032027.2. NM_008001.4.
UniGeneiMm.475704.
Mm.8142.

Genome annotation databases

EnsembliENSMUST00000026296; ENSMUSP00000026296; ENSMUSG00000025265.
GeneIDi14163.
KEGGimmu:14163.
UCSCiuc009uow.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22325 mRNA. Translation: AAA96001.1 .
AL805937 Genomic DNA. Translation: CAM14870.1 .
BC011462 mRNA. Translation: AAH11462.1 .
CCDSi CCDS30467.1.
RefSeqi NP_032027.2. NM_008001.4.
UniGenei Mm.475704.
Mm.8142.

3D structure databases

ProteinModelPortali P52734.
SMRi P52734. Positions 364-791, 830-919.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199637. 2 interactions.
IntActi P52734. 2 interactions.
MINTi MINT-272222.

PTM databases

PhosphoSitei P52734.

Proteomic databases

MaxQBi P52734.
PaxDbi P52734.
PRIDEi P52734.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026296 ; ENSMUSP00000026296 ; ENSMUSG00000025265 .
GeneIDi 14163.
KEGGi mmu:14163.
UCSCi uc009uow.1. mouse.

Organism-specific databases

CTDi 2245.
MGIi MGI:104566. Fgd1.

Phylogenomic databases

eggNOGi NOG295069.
GeneTreei ENSGT00750000117280.
HOGENOMi HOG000220866.
HOVERGENi HBG007506.
InParanoidi Q921L2.
KOi K05720.
OMAi LQMPRMP.
TreeFami TF316247.

Enzyme and pathway databases

Reactomei REACT_204733. G alpha (12/13) signalling events.
REACT_209641. NRAGE signals death through JNK.
REACT_210090. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSi FGD1. mouse.
NextBioi 285298.
PROi P52734.
SOURCEi Search...

Gene expression databases

Bgeei P52734.
CleanExi MM_FGD1.
Genevestigatori P52734.

Family and domain databases

Gene3Di 1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
3.30.40.10. 1 hit.
InterProi IPR000219. DH-domain.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
Pfami PF01363. FYVE. 1 hit.
PF00169. PH. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view ]
SMARTi SM00064. FYVE. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view ]
SUPFAMi SSF48065. SSF48065. 1 hit.
PROSITEi PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and regional localization of the mouse faciogenital dysplasia (Fgd1) gene."
    Pasteris N.G., de Gouyon B., Cadle A.B., Campbell K., Herman G.E., Gorski J.L.
    Mamm. Genome 6:658-661(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Fgd1, the Cdc42 GEF responsible for faciogenital dysplasia, directly interacts with cortactin and mAbp1 to modulate cell shape."
    Hou P., Estrada L., Kinley A.W., Parsons J.T., Vojtek A.B., Gorski J.L.
    Hum. Mol. Genet. 12:1981-1993(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DBNL AND CTTN, MUTAGENESIS OF PRO-159; PRO-162 AND LYS-164.
  5. "Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs."
    Kostenko E.V., Olabisi O.O., Sahay S., Rodriguez P.L., Whitehead I.P.
    Mol. Cell. Biol. 26:8964-8975(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE INTERACTION WITH CCPG1.

Entry informationi

Entry nameiFGD1_MOUSE
AccessioniPrimary (citable) accession number: P52734
Secondary accession number(s): Q921L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi