Reviewed, UniProtKB/Swiss-Prot P52731 (PDE6C_CHICK)

Names and origin

Protein names

Recommended name:
    Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'
      Short name=cGMP phosphodiesterase 6C
    EC=3.1.4.35

Gene names

Name:

PDE6C

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Subunit structure	Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Methylation Prenylation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 859

859

Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'

PRO_0000198832

Propeptide

860 – 862

3

Removed in mature form Potential

PRO_0000370790

Regions

Domain

75 – 224

150

GAF 1

Domain

256 – 433

178

GAF 2

Amino acid modifications

Modified residue

859

1

Cysteine methyl ester Potential

Lipidation

859

1

S-geranylgeranyl cysteine By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

862

Helix Strand Turn

Details...

Helix

56 – 66

11

Helix

76 – 91

16

Beta strand

93 – 103

11

Beta strand

108 – 117

10

Helix

123 – 126

4

Helix

130 – 132

3

Helix

146 – 149

4

Beta strand

153 – 156

4

Helix

158 – 160

3

Helix

167 – 171

5

Beta strand

179 – 185

7

Beta strand

190 – 204

15

Helix

207 – 223

17

Sequences

	Sequence		Length	Mass (Da)
	P52731-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: BD3145BB5FF826A8	FASTA	862	100,009
	10 20 30 40 50 60 MGEVNKDAVE KYLENNPQFA KEYFDRKMRA EVLGSIFQVS PGDVKEGVSF KDMSRLEECN 70 80 90 100 110 120 ILFELLTEIQ DEAGSMEKIV HKTLQRLSQL LARDRCSMFI CRSRNGIPEV ATRLLNVTPT 130 140 150 160 170 180 SKFEDNLVNP DKETVFPLDI GIAGWVAHTK KFFNIPDVKK NNHFSDYLDK KTGYTTVNMM 190 200 210 220 230 240 AIPITQGKEV LAVVMALNKL NASEFSKEDE EVFKKYLNFI SLVLRNHHTS YLYNIESRRS 250 260 270 280 290 300 QMLLWSANKV FEELTDIERQ FHKALYTIRM YLNCERYSVG LLDMTKEKEF YDEWPIRLGE 310 320 330 340 350 360 AEPYKGPKTP DGREVNFYKI IDYILHGKEE IKVIPTPPAD HWCLISGLPT YVAENGFICN 370 380 390 400 410 420 MMNAPADEYF TFQKGPVDET GWVIKNVLSL PIVNKKEEIV GVATFYNRKD GKPFDEYDEQ 430 440 450 460 470 480 IIETLTQFLG WSVLNTDTYD KMNKLENRKD IAQEMLMYQT KATPTEVESI LKYKEKLNVK 490 500 510 520 530 540 SIEECDEKDL IRILKEELPD PKDLELYEFR FSDFPVTEHG LITCGIRLFF EINVVEKFKV 550 560 570 580 590 600 PAEVLTRWMY TVRKGYRDIT YHNWRHGFNV GQTMFTLLMT GRIKKYYTDL EAFAMVAAAF 610 620 630 640 650 660 CHDIDHRGTN NLYQMKSAAP LAKLHGSSIL ERHHLEYSKT LLQDESLNIF QNLNKRQFET 670 680 690 700 710 720 VLHLFEVAII ATDLALYFKK RTMFQKIVDA IEKMETEEEA IKYISIDPTK KEVIMAMMMT 730 740 750 760 770 780 GCDLSAITKP WEVQSKVALM VANEFWEQGD LERTVLQQQP IPMMDRNKGD ELPKLQVGFI 790 800 810 820 830 840 DFVCTFVYKE FSRFHKEITP MFDGLQNNRV EWKTRADEYE EKMKVIEEQK KKEEEAAAKK 850 860 AENAAGGGGG GEDGKSKTCI VL

References

[1]	"Molecular characterization of the alpha'-subunit of cone photoreceptor cGMP phosphodiesterase in normal and rd chicken." Semple-Rowland S.L., Green D.A. Exp. Eye Res. 59:365-372(1994) [PubMed: 7821382] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Rhode Island red. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L29233 mRNA. Translation: AAC42223.1.

IPI

IPI00582931.

PIR

I50186.

RefSeq

NP_990317.1.

UniGene

Gga.492

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DBA	X-ray	2.57	A/B	55-225	[»]

ModBase

Search...

Proteomic databases

PRIDE

P52731.

Genome annotation databases

Ensembl

ENSGALT00000010702; ENSGALP00000010688; ENSGALG00000006626; Gallus gallus. [Genome view]

GeneID

395834.

KEGG

gga:395834.

Organism-specific databases

CTD

395834.

Phylogenomic databases

HOGENOM

P52731.

HOVERGEN

P52731.

Enzyme and pathway databases

BRENDA

3.1.4.35. 4.

Family and domain databases

InterPro

IPR003018. GAF.
IPR003607. Met-dep_phosphohydro_HD.
IPR002073. PDEase.
[Graphical view]

Pfam

PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]

PRINTS

PR00387. PDIESTERASE1.

SMART

SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]

PROSITE

PS00126. PDEASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PDE6C_CHICK

Accession

Primary (citable) accession number: P52731

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)