Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P52731

- PDE6C_CHICK

UniProt

P52731 - PDE6C_CHICK

Protein

Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'

Gene

PDE6C

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei97 – 971cGMP1 Publication
    Binding sitei116 – 1161cGMP1 Publication
    Binding sitei176 – 1761cGMP1 Publication
    Active sitei562 – 5621Proton donorBy similarity
    Metal bindingi566 – 5661Divalent metal cation 1By similarity
    Metal bindingi602 – 6021Divalent metal cation 1By similarity
    Metal bindingi603 – 6031Divalent metal cation 1By similarity
    Metal bindingi603 – 6031Divalent metal cation 2By similarity
    Metal bindingi723 – 7231Divalent metal cation 1By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi169 – 1724cGMP1 Publication

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
    2. cGMP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. signal transduction Source: InterPro
    2. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    cGMP, cGMP-binding, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha' (EC:3.1.4.35)
    Alternative name(s):
    cGMP phosphodiesterase 6C
    Gene namesi
    Name:PDE6C
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 859859Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'PRO_0000198832Add
    BLAST
    Propeptidei860 – 8623Removed in mature formSequence AnalysisPRO_0000370790

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei859 – 8591Cysteine methyl esterSequence Analysis
    Lipidationi859 – 8591S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    PaxDbiP52731.
    PRIDEiP52731.

    Interactioni

    Subunit structurei

    Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.1 Publication

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000010688.

    Structurei

    Secondary structure

    1
    862
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi56 – 6611
    Beta strandi71 – 744
    Helixi75 – 9117
    Beta strandi93 – 10412
    Beta strandi107 – 11711
    Helixi123 – 1264
    Helixi130 – 1323
    Helixi142 – 1498
    Beta strandi153 – 1564
    Helixi158 – 1603
    Helixi167 – 1726
    Beta strandi179 – 1868
    Beta strandi189 – 20416
    Helixi207 – 22317

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3DBAX-ray2.57A/B55-225[»]
    ProteinModelPortaliP52731.
    SMRiP52731. Positions 55-225.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP52731.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini75 – 224150GAF 1Add
    BLAST
    Domaini256 – 433178GAF 2Add
    BLAST

    Sequence similaritiesi

    Contains 2 GAF domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG290744.
    HOGENOMiHOG000007069.
    HOVERGENiHBG053539.
    InParanoidiP52731.
    KOiK13757.
    PhylomeDBiP52731.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 3 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52731-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGEVNKDAVE KYLENNPQFA KEYFDRKMRA EVLGSIFQVS PGDVKEGVSF    50
    KDMSRLEECN ILFELLTEIQ DEAGSMEKIV HKTLQRLSQL LARDRCSMFI 100
    CRSRNGIPEV ATRLLNVTPT SKFEDNLVNP DKETVFPLDI GIAGWVAHTK 150
    KFFNIPDVKK NNHFSDYLDK KTGYTTVNMM AIPITQGKEV LAVVMALNKL 200
    NASEFSKEDE EVFKKYLNFI SLVLRNHHTS YLYNIESRRS QMLLWSANKV 250
    FEELTDIERQ FHKALYTIRM YLNCERYSVG LLDMTKEKEF YDEWPIRLGE 300
    AEPYKGPKTP DGREVNFYKI IDYILHGKEE IKVIPTPPAD HWCLISGLPT 350
    YVAENGFICN MMNAPADEYF TFQKGPVDET GWVIKNVLSL PIVNKKEEIV 400
    GVATFYNRKD GKPFDEYDEQ IIETLTQFLG WSVLNTDTYD KMNKLENRKD 450
    IAQEMLMYQT KATPTEVESI LKYKEKLNVK SIEECDEKDL IRILKEELPD 500
    PKDLELYEFR FSDFPVTEHG LITCGIRLFF EINVVEKFKV PAEVLTRWMY 550
    TVRKGYRDIT YHNWRHGFNV GQTMFTLLMT GRIKKYYTDL EAFAMVAAAF 600
    CHDIDHRGTN NLYQMKSAAP LAKLHGSSIL ERHHLEYSKT LLQDESLNIF 650
    QNLNKRQFET VLHLFEVAII ATDLALYFKK RTMFQKIVDA IEKMETEEEA 700
    IKYISIDPTK KEVIMAMMMT GCDLSAITKP WEVQSKVALM VANEFWEQGD 750
    LERTVLQQQP IPMMDRNKGD ELPKLQVGFI DFVCTFVYKE FSRFHKEITP 800
    MFDGLQNNRV EWKTRADEYE EKMKVIEEQK KKEEEAAAKK AENAAGGGGG 850
    GEDGKSKTCI VL 862
    Length:862
    Mass (Da):100,009
    Last modified:October 1, 1996 - v1
    Checksum:iBD3145BB5FF826A8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L29233 mRNA. Translation: AAC42223.1.
    PIRiI50186.
    RefSeqiNP_990317.1. NM_204986.1.
    UniGeneiGga.492.

    Genome annotation databases

    GeneIDi395834.
    KEGGigga:395834.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L29233 mRNA. Translation: AAC42223.1 .
    PIRi I50186.
    RefSeqi NP_990317.1. NM_204986.1.
    UniGenei Gga.492.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3DBA X-ray 2.57 A/B 55-225 [» ]
    ProteinModelPortali P52731.
    SMRi P52731. Positions 55-225.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000010688.

    Proteomic databases

    PaxDbi P52731.
    PRIDEi P52731.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 395834.
    KEGGi gga:395834.

    Organism-specific databases

    CTDi 5146.

    Phylogenomic databases

    eggNOGi NOG290744.
    HOGENOMi HOG000007069.
    HOVERGENi HBG053539.
    InParanoidi P52731.
    KOi K13757.
    PhylomeDBi P52731.

    Miscellaneous databases

    EvolutionaryTracei P52731.
    NextBioi 20815902.
    PROi P52731.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProi IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55781. SSF55781. 3 hits.
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the alpha'-subunit of cone photoreceptor cGMP phosphodiesterase in normal and rd chicken."
      Semple-Rowland S.L., Green D.A.
      Exp. Eye Res. 59:365-372(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Rhode Island red.
      Tissue: Retina.
    2. "The structure of the GAF A domain from phosphodiesterase 6C reveals determinants of cGMP binding, a conserved binding surface, and a large cGMP-dependent conformational change."
      Martinez S.E., Heikaus C.C., Klevit R.E., Beavo J.A.
      J. Biol. Chem. 283:25913-25919(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.57 ANGSTROMS) OF 55-225 IN COMPLEX WITH CGMP.

    Entry informationi

    Entry nameiPDE6C_CHICK
    AccessioniPrimary (citable) accession number: P52731
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3