Reviewed, UniProtKB/Swiss-Prot P52713 (MMSA_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
      Short name=MMSDH
      Short name=Malonate-semialdehyde dehydrogenase [acylating]
    EC=1.2.1.27
    EC=1.2.1.18

Gene names

Name:	alh-8
ORF Names:	F13D12.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA By similarity.
Catalytic activity	2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. 3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW thymine metabolic process Inferred from sequence or structural similarity. Source: UniProtKB valine metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl-CoA binding Inferred from sequence or structural similarity. Source: UniProtKB malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from sequence or structural similarity. Source: UniProtKB methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion By similarity
	Chain	23 – 523	501	Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial		PRO_0000007191
Regions
	Nucleotide binding	199 – 203	5	NAD Potential
	Nucleotide binding	251 – 256	6	NAD Potential
Sites
	Active site	307	1	Nucleophile By similarity
	Binding site	407	1	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P52713-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2871499FE3267A29	FASTA	523	56,462
	10 20 30 40 50 60 MLSRLARVQP KCQQLAHFST SKSAAAAPTV KLWIDGQAVE SKTTDFVELT NPATNEVIAM 70 80 90 100 110 120 VPNATQAEMQ AAVDSAKNAF NTWKNTSPLT RQQCMFKLQA LIKRDMKKLA ESITIEQGKT 130 140 150 160 170 180 LPDAEGDVSR GLQVVEHACS VPSLMMGETL PNVSRDMDTH SYRIPLGVTA GICPFNFPAM 190 200 210 220 230 240 IPLWMFPVAL ATGNTMVIKP SEQDPGAAQL LVELAKEAGV PDGCVNIIHG QHSAVNFICD 250 260 270 280 290 300 NPDIKAISFV GGDAAGKHIY ERGAKNGKRV QSNMGAKNHG VIMADANKEQ TLNQLTAAAF 310 320 330 340 350 360 GAAGQRCMAL TTAVLVGEAR AWLPELVEKA KNLKVNAGWK PDTDIGPLIS KQSKARVLRL 370 380 390 400 410 420 IESAKKEGAQ VPLDGSNITV PGFENGNFVG PTILAGVKPN MTCYREEIFG PVLVVMEAEN 430 440 450 460 470 480 LNEAIEIINN NPYGNGTAIF TSNGATARKF TNEVDVGQIG INVPIPVPLP MFSFTGSRGS 490 500 510 520 FLGDLNFYGK AGIQFYTQWK TVTQYWNESL TELKPQMSFP QLK

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49127 Genomic DNA. Translation: CAA88946.1.
PIR	T20828.
RefSeq	NP_001022078.1.
UniGene	Cel.17786
3D structure databases
HSSP	HSSP built from PDB template 1O9J based on UniProtKB Q28399.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:25038N.
IntAct	P52713. 1 interaction.
STRING	P52713.
Proteomic databases
PRIDE	P52713.
Genome annotation databases
Ensembl	F13D12.4a.1; F13D12.4a.1; F13D12.4; Caenorhabditis elegans. [Genome view] F13D12.4a.2; F13D12.4a.2; F13D12.4; Caenorhabditis elegans. [Genome view] F13D12.4a.3; F13D12.4a.3; F13D12.4; Caenorhabditis elegans. [Genome view]
GeneID	174800.
KEGG	cel:F13D12.4.
UCSC	F13D12.4a.2. c. elegans.
Organism-specific databases
CTD	174800.
WormBase	WBGene00000114. alh-8.
WormPep	F13D12.4. CE02183. [WorfDB]
Phylogenomic databases
OMA	AIFVGES.
Enzyme and pathway databases
BRENDA	1.2.1.18. 672. 1.2.1.27. 672.
Gene expression databases
ArrayExpress	P52713.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	885562.

Entry information

Entry name

MMSA_CAEEL

Accession

Primary (citable) accession number: P52713

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project