P52709 (SYTC_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase, cytoplasmic
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	trs-1
ORF Names:	C47D12.6

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	determination of adult lifespan Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Threonine--tRNA ligase, cytoplasmic		PRO_0000101122

Sequences

	Sequence		Length	Mass (Da)
	P52709 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C234CCB61CDF5BDA	FASTA	725	84,417
	10 20 30 40 50 60 MRLNCFRIFV HIQKPTQIFK PFYRSLSSEA SDKYHFVNGH KMSKAPTDMA PWPAFIEERI 70 80 90 100 110 120 KLWDKLKAEY DAEIAAKESE PIQITLPDGK IHEGKTWRTT PFEIAERISK GLAEAAVIAK 130 140 150 160 170 180 VNGAVWDLDR PFEGNAKLEL LKFDDDEAKQ VFWHSSAHVL GEAMERYCGG HLCYGPPIQE 190 200 210 220 230 240 GFYYDMWHEN RTICPDDFPK IDQIVKAAVK DKQKFERLEM TKEDLLEMFK YNEFKVRIIT 250 260 270 280 290 300 EKIHTPKTTV YRCGPLIDLC RGPHVRHTGK VKAMAITKNS SSYWEGKADA ESLQRLYGIS 310 320 330 340 350 360 FPDSKQLKEW QKLQEEAAKR DHRKLGKEHD LFFFHQLSPG SAFWYPKGAH IYNKLVDFIR 370 380 390 400 410 420 KQYRRRGFTE VITPNMYNKK LWETSGHWQH YSEDMFKIEV EKEEFGLKPM NCPGHCLMFG 430 440 450 460 470 480 HMPHTYNELP FRFADFGVLH RNEMSGALTG LTRVRRFQQD DAHIFCRQDQ ISEEIKQCLD 490 500 510 520 530 540 FLEYAYEKVF GFTFKLNLST RPEGFLGNIE TWDKAEADLT NALNASGRKW VLNPGDGAFY 550 560 570 580 590 600 GPKIDITIQD ALKRNFQCAT IQLDFQLPNQ FDLSYFDEKG EKQRPVMIHR AVLGSVERMT 610 620 630 640 650 660 AILTESYGGK WPFWLSPRQC KIITVHESVR DYANDVKKQI FEAGFEIEYE ENCGDTMNKQ 670 680 690 700 710 720 VRKAQLAQFN FILVIGAKEK ENGTVNVRTR DNAVRGEVAL DKLISKFRRF ADEYVADTEK SEEWA

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z69902 Genomic DNA. Translation: CAA93762.1.
PIR	T19994.
RefSeq	NP_001022033.1. NM_001026862.2.
UniGene	Cel.17250.
3D structure databases
ProteinModelPortal	P52709.
SMR	P52709. Positions 75-719.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24299N.
MINT	MINT-1112314.
STRING	P52709.
Proteomic databases
PRIDE	P52709.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C47D12.6a; C47D12.6a; C47D12.6.
GeneID	174790.
KEGG	cel:C47D12.6.
UCSC	C47D12.6b.1. c. elegans.
Organism-specific databases
CTD	174790.
WormBase	C47D12.6a; CE05434; WBGene00006617; tars-1.
Phylogenomic databases
eggNOG	meNOG04331.
HOGENOM	HBG352811.
InParanoid	P52709.
OMA	HYAENMF.
PhylomeDB	P52709.
Gene expression databases
ArrayExpress	P52709.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	885520.

Entry information

Entry name

SYTC_CAEEL

Accession

Primary (citable) accession number: P52709

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project