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P52707

- MDL3_PRUSE

UniProt

P52707 - MDL3_PRUSE

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Protein
(R)-mandelonitrile lyase 3
Gene
MDL3
Organism
Prunus serotina (Black cherry)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in cyanogenesis, the release of HCN from injured tissues. Catalyzes the stereospecific addition of HCN to a variety of aldehydes in vitro. It is a major seed constituent, and could have the additional role of a storage form for reduced nitrogen.

Catalytic activityi

(R)-mandelonitrile = cyanide + benzaldehyde.

Cofactori

FAD.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei129 – 1291FAD; via carbonyl oxygen By similarity
Binding sitei133 – 1331FAD By similarity
Binding sitei244 – 2441FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei356 – 3561Substrate By similarity
Binding sitei485 – 4851Substrate By similarity
Active sitei487 – 4871Proton donor By similarity
Binding sitei515 – 5151FAD; via amide nitrogen By similarity
Active sitei525 – 5251Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 642FAD By similarity
Nucleotide bindingi82 – 832FAD By similarity
Nucleotide bindingi137 – 1404FAD By similarity
Nucleotide bindingi486 – 4872FAD By similarity
Nucleotide bindingi526 – 5272FAD By similarity

GO - Molecular functioni

  1. choline dehydrogenase activity Source: InterPro
  2. flavin adenine dinucleotide binding Source: InterPro
  3. mandelonitrile lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. alcohol metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
(R)-mandelonitrile lyase 3 (EC:4.1.2.10)
Alternative name(s):
Hydroxynitrile lyase 3
Short name:
(R)-oxynitrilase 3
Gene namesi
Name:MDL3
OrganismiPrunus serotina (Black cherry)
Taxonomic identifieri23207 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeAmygdaleaePrunus

Subcellular locationi

Vacuolealeurone grain By similarity
Note: Primarily found within protein bodies of the cotyledonary parenchyma cells, with lesser amounts within the procambium By similarity.

GO - Cellular componenti

  1. aleurone grain Source: UniProtKB-SubCell
  2. vacuole Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed prediction
Add
BLAST
Chaini28 – 573546(R)-mandelonitrile lyase 3
PRO_0000012342Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi30 – 301N-linked (GlcNAc...) Reviewed prediction
Glycosylationi75 – 751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi145 – 1451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi150 – 1501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi162 – 1621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi218 – 2181N-linked (GlcNAc...) Reviewed prediction
Glycosylationi252 – 2521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi267 – 2671N-linked (GlcNAc...) Reviewed prediction
Glycosylationi309 – 3091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi380 – 3801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi402 – 4021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi420 – 4201N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi427 ↔ 478 By similarity
Glycosylationi467 – 4671N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP52707.
SMRiP52707. Positions 28-549.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi293 – 2964Poly-Leu

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
PROSITEiPS00623. GMC_OXRED_1. 1 hit.
PS00624. GMC_OXRED_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52707-1 [UniParc]FASTAAdd to Basket

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MVKSTMSAVL LVLHIFVLHL QYSEVQSLAN TSSHDFSYLS FVYDATDPEL    50
EGSYDYIIVG GGTAGCPLAA TLSANYSVLV LERGSLPTEY PNLLISDGFV 100
YNLQQEDDGK TPVERFVSED GIDNVRGRVL GGTSMINAGV YVRANTSFFN 150
QTGIEWDMDL VNQTYEWVED TIVFEPDSQT WQTVIGTAYL EAGILPNNGF 200
SVDHLAGTRL TGSTFDNNGT RHASDELLNK GDPNNLRVAV QAAVEKIIFS 250
SNTSGVTAIG VIYTDSNGTT HQAFVRGEGE VILSAGPIGS PQLLLLSGVG 300
PESYLTSLNI SVVASHPYVG QYIYDNPRNF INILPPNPIE ASTVTVLGIT 350
SDFYQCSISS LPFDTPPFSF FPTTSYPLPN QTFAHIVNKV PGPLSHGTVT 400
LNSSSDVRVG PNVKFNYYSN LTDLSHCVSG MKKLGEVLST DALEPYKVED 450
LPGIDGFNIL GIPLPENQTD DAAFETFCRE SVASYWHYHG GCLVGKVLDD 500
GFRVTGINAL RVVDGSTFPS TPASHPQGFY LMLGRYMGIQ ILQERSASED 550
AIRNLGFQEN ILDSPKSTSS FAF 573
Length:573
Mass (Da):62,180
Last modified:October 1, 1996 - v1
Checksum:iDB181C68FED3F800
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51562 Genomic DNA. Translation: AAA96782.1.
AF013161 mRNA. Translation: AAB67714.1.
PIRiT07948.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51562 Genomic DNA. Translation: AAA96782.1 .
AF013161 mRNA. Translation: AAB67714.1 .
PIRi T07948.

3D structure databases

ProteinModelPortali P52707.
SMRi P52707. Positions 28-549.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view ]
Pfami PF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000137. Alcohol_oxidase. 1 hit.
PROSITEi PS00623. GMC_OXRED_1. 1 hit.
PS00624. GMC_OXRED_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequencing, genomic organization, and preliminary promoter analysis of a black cherry (R)-(+)-mandelonitrile lyase gene."
    Hu Z., Poulton J.E.
    Plant Physiol. 115:1359-1369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Entry informationi

Entry nameiMDL3_PRUSE
AccessioniPrimary (citable) accession number: P52707
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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