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P52706

- MDL1_PRUSE

UniProt

P52706 - MDL1_PRUSE

Protein

(R)-mandelonitrile lyase 1

Gene

MDL1

Organism
Prunus serotina (Black cherry)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in cyanogenesis, the release of HCN from injured tissues. Catalyzes the stereospecific addition of HCN to a variety of aldehydes in vitro. It is a major seed constituent, and could have the additional role of a storage form for reduced nitrogen.

    Catalytic activityi

    (R)-mandelonitrile = cyanide + benzaldehyde.

    Cofactori

    FAD.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei129 – 1291FAD; via carbonyl oxygenBy similarity
    Binding sitei133 – 1331FADBy similarity
    Binding sitei244 – 2441FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei355 – 3551SubstrateBy similarity
    Binding sitei484 – 4841SubstrateBy similarity
    Active sitei486 – 4861Proton donorBy similarity
    Binding sitei514 – 5141FAD; via amide nitrogenBy similarity
    Active sitei524 – 5241Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi63 – 642FADBy similarity
    Nucleotide bindingi82 – 832FADBy similarity
    Nucleotide bindingi137 – 1404FADBy similarity
    Nucleotide bindingi485 – 4862FADBy similarity
    Nucleotide bindingi525 – 5262FADBy similarity

    GO - Molecular functioni

    1. choline dehydrogenase activity Source: InterPro
    2. flavin adenine dinucleotide binding Source: InterPro
    3. mandelonitrile lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. alcohol metabolic process Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    (R)-mandelonitrile lyase 1 (EC:4.1.2.10)
    Alternative name(s):
    Hydroxynitrile lyase 1
    Short name:
    (R)-oxynitrilase 1
    Gene namesi
    Name:MDL1
    OrganismiPrunus serotina (Black cherry)
    Taxonomic identifieri23207 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeAmygdaleaePrunus

    Subcellular locationi

    Vacuolealeurone grain
    Note: Primarily found within protein bodies of the cotyledonary parenchyma cells, with lesser amounts within the procambium.

    GO - Cellular componenti

    1. aleurone grain Source: UniProtKB-SubCell
    2. vacuole Source: UniProtKB-KW

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 27271 PublicationAdd
    BLAST
    Chaini28 – 563536(R)-mandelonitrile lyase 1PRO_0000012340Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi379 – 3791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi426 ↔ 477By similarity

    Post-translational modificationi

    Glycosylated.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Seeds. Localized within cotyledonary parenchyma cells.1 Publication

    Developmental stagei

    Absent from maturing black cherry fruits until 6 weeks after flowering. Then, concomitant with cotyledon development, the level of enzyme increases with specificity for embryonal tissues.2 Publications

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP52706.
    SMRiP52706. Positions 28-548.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi292 – 2954Poly-Leu

    Sequence similaritiesi

    Belongs to the GMC oxidoreductase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR012132. GMC_OxRdtase.
    IPR000172. GMC_OxRdtase_N.
    IPR007867. GMC_OxRtase_C.
    [Graphical view]
    PfamiPF05199. GMC_oxred_C. 1 hit.
    PF00732. GMC_oxred_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
    PROSITEiPS00623. GMC_OXRED_1. 1 hit.
    PS00624. GMC_OXRED_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52706-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKSTMSAIL LVLHLFVLLL QYSEVHSLAT TSNHDFSYLR FAYDATDLEL    50
    EGSYDYVIVG GGTSGCPLAA TLSEKYKVLV LERGSLPTAY PNVLTADGFV 100
    YNLQQEDDGK TPVERFVSED GIDNVRGRVL GGTSMINAGV YARANTSIYS 150
    ASGVDWDMDL VNKTYEWVED TIVFKPNYQP WQSVTGTAFL EAGVDPNHGF 200
    SLDHEAGTRI TGSTFDNKGT RHAADELLNK GNSNNLRVGV HASVEKIIFS 250
    NAPGLTATGV IYRDSNGTPH RAFVRSKGEV IVSAGTIGTP QLLLLSGVGP 300
    ESYLSSLNIP VVLSHPYVGQ FLHDNPRNFI NILPPNPIEP TIVTVLGISN 350
    DFYQCSFSSL PFTTPPFSFF PSTSYPLPNS TFAHFASKVA GPLSYGSLTL 400
    KSSSNVRVSP NVKFNYYSNP TDLSHCVSGM KKIGELLSTD ALKPYKVEDL 450
    PGIEGFNILG IPLPKDQTDD AAFETFCRES VASYWHYHGG CLVGKVLDGD 500
    FRVTGIDALR VVDGSTFPYT PASHPQGFYL MLGRYVGIKI LQERSASDLK 550
    ILDSLKSAAS LVL 563
    Length:563
    Mass (Da):61,200
    Last modified:October 1, 1996 - v1
    Checksum:i152367E736AF5FDD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72617 mRNA. Translation: CAA51194.1.
    U78814 Genomic DNA. Translation: AAB38536.1.
    PIRiS32156.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72617 mRNA. Translation: CAA51194.1 .
    U78814 Genomic DNA. Translation: AAB38536.1 .
    PIRi S32156.

    3D structure databases

    ProteinModelPortali P52706.
    SMRi P52706. Positions 28-548.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR012132. GMC_OxRdtase.
    IPR000172. GMC_OxRdtase_N.
    IPR007867. GMC_OxRtase_C.
    [Graphical view ]
    Pfami PF05199. GMC_oxred_C. 1 hit.
    PF00732. GMC_oxred_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000137. Alcohol_oxidase. 1 hit.
    PROSITEi PS00623. GMC_OXRED_1. 1 hit.
    PS00624. GMC_OXRED_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of cDNA of Prunus serotina (R)-(+)-mandelonitrile lyase and identification of a putative FAD-binding site."
      Cheng I.-P., Poulton J.E.
      Plant Cell Physiol. 34:1139-1143(1993)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Seed.
    2. Hu Z., Poulton J.E.
      Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Immunocytochemical localization of mandelonitrile lyase in mature black cherry (Prunus serotina Ehrh.) seeds."
      Wu H.-C., Poulton J.E.
      Plant Physiol. 96:1329-1337(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-43, DEVELOPMENTAL STAGE.
      Tissue: Seed.
    4. "Temporal and spatial expression of amygdalin hydrolase and (R)-(+)-mandelonitrile lyase in black cherry seeds."
      Zheng L., Poulton J.E.
      Plant Physiol. 109:31-39(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMDL1_PRUSE
    AccessioniPrimary (citable) accession number: P52706
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3