P52699 (BLAB_SERMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-lactamase IMP-1 EC=3.5.2.6 Alternative name(s): BLAIMP Beta-lactamase type II Penicillinase
Organism	Serratia marcescens
Taxonomic identifier	615 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Confers resistance to imipenem and broad-spectrum beta-lactams. Also hydrolyzes carbapenems.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Sequence similarities	Belongs to the class-B beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Potential

Chain

19 – 246

228

Beta-lactamase IMP-1

PRO_0000016946

Sites

Metal binding

95

1

Zinc 1 By similarity

Metal binding

97

1

Zinc 1 By similarity

Metal binding

99

1

Zinc 2 By similarity

Metal binding

157

1

Zinc 1 By similarity

Metal binding

176

1

Zinc 2 By similarity

Metal binding

215

1

Zinc 2 By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

246

Helix Strand Turn

Details...

Beta strand

26 – 31

6

Beta strand

34 – 42

9

Beta strand

44 – 57

14

Beta strand

59 – 66

8

Helix

71 – 82

12

Turn

83 – 85

3

Beta strand

87 – 92

6

Beta strand

94 – 97

4

Helix

98 – 101

4

Helix

104 – 109

6

Beta strand

114 – 117

4

Helix

118 – 126

9

Beta strand

133 – 136

4

Beta strand

138 – 144

7

Turn

145 – 147

3

Beta strand

148 – 151

4

Beta strand

155 – 158

4

Beta strand

163 – 166

4

Turn

167 – 170

4

Beta strand

171 – 175

5

Turn

191 – 193

3

Helix

194 – 204

11

Beta strand

209 – 216

8

Helix

222 – 236

15

Sequences

	Sequence		Length	Mass (Da)
	P52699 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 9B2599E8F1B22D36	FASTA	246	27,120
	10 20 30 40 50 60 MSKLSVFFIF LFCSIATAAE SLPDLKIEKL DEGVYVHTSF EEVNGWGVVP KHGLVVLVNA 70 80 90 100 110 120 EAYLIDTPFT AKDTEKLVTW FVERGYKIKG SISSHFHSDS TGGIEWLNSR SIPTYASELT 130 140 150 160 170 180 NELLKKDGKV QATNSFSGVN YWLVKNKIEV FYPGPGHTPD NVVVWLPERK ILFGGCFIKP 190 200 210 220 230 240 YGLGNLGDAN IEAWPKSAKL LKSKYGKAKL VVPSHSEVGD ASLLKLTLEQ AVKGLNESKK PSKPSN

References

[1]	"Molecular characterization of an enterobacterial metallo beta-lactamase found in a clinical isolate of Serratia marcescens that shows imipenem resistance." Osano E., Arakawa Y., Wacharotayankun R., Ohta M., Horii T., Ito H., Yoshimura F., Kato N. Antimicrob. Agents Chemother. 38:71-78(1994) [PubMed: 8141584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AK9373 / TN9106.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

S71932 Genomic DNA. Translation: AAB30289.1.
D50438 Genomic DNA. Translation: BAA08930.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DD6	X-ray	2.00	A/B	19-246	[»]
1VGN	X-ray	2.63	A/B	19-246	[»]
1WUO	X-ray	2.01	A/B/C/D	19-246	[»]
1WUP	X-ray	3.00	A/B/C/D	19-246	[»]
2DOO	X-ray	2.43	A/B	19-246	[»]

ProteinModelPortal

P52699.

SMR

P52699. Positions 19-239.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001279. Beta-lactamas-like.
IPR001018. Beta-lactamase_class-B_CS.
[Graphical view]

Pfam

PF00753. Lactamase_B. 1 hit.
[Graphical view]

SMART

SM00849. Lactamase_B. 1 hit.
[Graphical view]

PROSITE

PS00743. BETA_LACTAMASE_B_1. 1 hit.
PS00744. BETA_LACTAMASE_B_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BLAB_SERMA

Accession

Primary (citable) accession number: P52699

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	September 21, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program