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P52699

- BLAB_SERMA

UniProt

P52699 - BLAB_SERMA

Protein

Beta-lactamase IMP-1

Gene
N/A
Organism
Serratia marcescens
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Confers resistance to imipenem and broad-spectrum beta-lactams. Also hydrolyzes carbapenems.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi95 – 951Zinc 1By similarity
    Metal bindingi97 – 971Zinc 1By similarity
    Metal bindingi99 – 991Zinc 2By similarity
    Metal bindingi157 – 1571Zinc 1By similarity
    Metal bindingi176 – 1761Zinc 2By similarity
    Metal bindingi215 – 2151Zinc 2By similarity

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase IMP-1 (EC:3.5.2.6)
    Alternative name(s):
    BLAIMP
    Beta-lactamase type II
    Penicillinase
    OrganismiSerratia marcescens
    Taxonomic identifieri615 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 246228Beta-lactamase IMP-1PRO_0000016946Add
    BLAST

    Interactioni

    Structurei

    Secondary structure

    1
    246
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi26 – 316
    Beta strandi34 – 4310
    Turni44 – 463
    Beta strandi47 – 5812
    Beta strandi61 – 666
    Helixi71 – 8212
    Turni83 – 853
    Beta strandi87 – 926
    Beta strandi94 – 974
    Helixi98 – 1014
    Helixi104 – 1096
    Beta strandi114 – 1174
    Helixi118 – 1269
    Beta strandi133 – 1364
    Beta strandi138 – 1447
    Turni145 – 1473
    Beta strandi148 – 1514
    Beta strandi155 – 1606
    Beta strandi163 – 1664
    Turni167 – 1704
    Beta strandi171 – 1755
    Turni191 – 1933
    Helixi194 – 20512
    Beta strandi209 – 2168
    Helixi222 – 23615

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DD6X-ray2.00A/B19-246[»]
    1VGNX-ray2.63A/B19-246[»]
    1WUOX-ray2.01A/B/C/D19-246[»]
    1WUPX-ray3.00A/B/C/D19-246[»]
    2DOOX-ray2.43A/B19-246[»]
    ProteinModelPortaliP52699.
    SMRiP52699. Positions 19-239.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP52699.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.60.15.10. 1 hit.
    InterProiIPR001279. Beta-lactamas-like.
    IPR001018. Beta-lactamase_class-B_CS.
    [Graphical view]
    PfamiPF00753. Lactamase_B. 1 hit.
    [Graphical view]
    SMARTiSM00849. Lactamase_B. 1 hit.
    [Graphical view]
    SUPFAMiSSF56281. SSF56281. 1 hit.
    PROSITEiPS00743. BETA_LACTAMASE_B_1. 1 hit.
    PS00744. BETA_LACTAMASE_B_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52699-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKLSVFFIF LFCSIATAAE SLPDLKIEKL DEGVYVHTSF EEVNGWGVVP    50
    KHGLVVLVNA EAYLIDTPFT AKDTEKLVTW FVERGYKIKG SISSHFHSDS 100
    TGGIEWLNSR SIPTYASELT NELLKKDGKV QATNSFSGVN YWLVKNKIEV 150
    FYPGPGHTPD NVVVWLPERK ILFGGCFIKP YGLGNLGDAN IEAWPKSAKL 200
    LKSKYGKAKL VVPSHSEVGD ASLLKLTLEQ AVKGLNESKK PSKPSN 246
    Length:246
    Mass (Da):27,120
    Last modified:October 1, 1996 - v1
    Checksum:i9B2599E8F1B22D36
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S71932 Genomic DNA. Translation: AAB30289.1.
    D50438 Genomic DNA. Translation: BAA08930.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S71932 Genomic DNA. Translation: AAB30289.1 .
    D50438 Genomic DNA. Translation: BAA08930.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DD6 X-ray 2.00 A/B 19-246 [» ]
    1VGN X-ray 2.63 A/B 19-246 [» ]
    1WUO X-ray 2.01 A/B/C/D 19-246 [» ]
    1WUP X-ray 3.00 A/B/C/D 19-246 [» ]
    2DOO X-ray 2.43 A/B 19-246 [» ]
    ProteinModelPortali P52699.
    SMRi P52699. Positions 19-239.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P52699.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P52699.

    Family and domain databases

    Gene3Di 3.60.15.10. 1 hit.
    InterProi IPR001279. Beta-lactamas-like.
    IPR001018. Beta-lactamase_class-B_CS.
    [Graphical view ]
    Pfami PF00753. Lactamase_B. 1 hit.
    [Graphical view ]
    SMARTi SM00849. Lactamase_B. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56281. SSF56281. 1 hit.
    PROSITEi PS00743. BETA_LACTAMASE_B_1. 1 hit.
    PS00744. BETA_LACTAMASE_B_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of an enterobacterial metallo beta-lactamase found in a clinical isolate of Serratia marcescens that shows imipenem resistance."
      Osano E., Arakawa Y., Wacharotayankun R., Ohta M., Horii T., Ito H., Yoshimura F., Kato N.
      Antimicrob. Agents Chemother. 38:71-78(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AK9373 / TN9106.

    Entry informationi

    Entry nameiBLAB_SERMA
    AccessioniPrimary (citable) accession number: P52699
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3