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P52699 (BLAB_SERMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase IMP-1

EC=3.5.2.6
Alternative name(s):
BLAIMP
Beta-lactamase type II
Penicillinase
OrganismSerratia marcescens
Taxonomic identifier615 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Confers resistance to imipenem and broad-spectrum beta-lactams. Also hydrolyzes carbapenems.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. Class-B beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processantibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 246228Beta-lactamase IMP-1
PRO_0000016946

Sites

Metal binding951Zinc 1 By similarity
Metal binding971Zinc 1 By similarity
Metal binding991Zinc 2 By similarity
Metal binding1571Zinc 1 By similarity
Metal binding1761Zinc 2 By similarity
Metal binding2151Zinc 2 By similarity

Secondary structure

......................................... 246
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P52699 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 9B2599E8F1B22D36

FASTA24627,120
        10         20         30         40         50         60 
MSKLSVFFIF LFCSIATAAE SLPDLKIEKL DEGVYVHTSF EEVNGWGVVP KHGLVVLVNA 

        70         80         90        100        110        120 
EAYLIDTPFT AKDTEKLVTW FVERGYKIKG SISSHFHSDS TGGIEWLNSR SIPTYASELT 

       130        140        150        160        170        180 
NELLKKDGKV QATNSFSGVN YWLVKNKIEV FYPGPGHTPD NVVVWLPERK ILFGGCFIKP 

       190        200        210        220        230        240 
YGLGNLGDAN IEAWPKSAKL LKSKYGKAKL VVPSHSEVGD ASLLKLTLEQ AVKGLNESKK 


PSKPSN 

« Hide

References

[1]"Molecular characterization of an enterobacterial metallo beta-lactamase found in a clinical isolate of Serratia marcescens that shows imipenem resistance."
Osano E., Arakawa Y., Wacharotayankun R., Ohta M., Horii T., Ito H., Yoshimura F., Kato N.
Antimicrob. Agents Chemother. 38:71-78(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: AK9373 / TN9106.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S71932 Genomic DNA. Translation: AAB30289.1.
D50438 Genomic DNA. Translation: BAA08930.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DD6X-ray2.00A/B19-246[»]
1VGNX-ray2.63A/B19-246[»]
1WUOX-ray2.01A/B/C/D19-246[»]
1WUPX-ray3.00A/B/C/D19-246[»]
2DOOX-ray2.43A/B19-246[»]
ProteinModelPortalP52699.
SMRP52699. Positions 19-239.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP52699.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.60.15.10. 1 hit.
InterProIPR001279. Beta-lactamas-like.
IPR001018. Beta-lactamase_class-B_CS.
[Graphical view]
PfamPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMSSF56281. SSF56281. 1 hit.
PROSITEPS00743. BETA_LACTAMASE_B_1. 1 hit.
PS00744. BETA_LACTAMASE_B_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP52699.

Entry information

Entry nameBLAB_SERMA
AccessionPrimary (citable) accession number: P52699
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references