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Protein

6-phosphogluconolactonase

Gene

pgl

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.1 Publication

Catalytic activityi

6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate.

Pathwayi: pentose phosphate pathway

This protein is involved in step 2 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (zwf)
  2. 6-phosphogluconolactonase (pgl)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (gnd)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

GO - Molecular functioni

  • 6-phosphogluconolactonase activity Source: EcoliWiki

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Enzyme and pathway databases

BioCyciEcoCyc:6PGLUCONOLACT-MONOMER.
ECOL316407:JW0750-MONOMER.
MetaCyc:6PGLUCONOLACT-MONOMER.
UniPathwayiUPA00115; UER00409.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphogluconolactonase (EC:3.1.1.31)
Short name:
6-P-gluconolactonase
Short name:
Pgl
Gene namesi
Name:pgl
Synonyms:ybhE
Ordered Locus Names:b0767, JW0750
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13231. pgl.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001711321 – 3316-phosphogluconolactonaseAdd BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei287N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP52697.
PaxDbiP52697.
PRIDEiP52697.

Interactioni

Protein-protein interaction databases

BioGridi4261151. 18 interactors.
IntActiP52697. 5 interactors.
STRINGi511145.b0767.

Structurei

Secondary structure

1331
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 9Combined sources8
Helixi10 – 12Combined sources3
Beta strandi14 – 20Combined sources7
Beta strandi26 – 33Combined sources8
Beta strandi41 – 43Combined sources3
Beta strandi47 – 54Combined sources8
Turni55 – 58Combined sources4
Beta strandi59 – 65Combined sources7
Turni67 – 69Combined sources3
Beta strandi72 – 79Combined sources8
Beta strandi85 – 89Combined sources5
Beta strandi93 – 100Combined sources8
Turni101 – 104Combined sources4
Beta strandi105 – 112Combined sources8
Beta strandi115 – 123Combined sources9
Beta strandi138 – 145Combined sources8
Helixi146 – 148Combined sources3
Beta strandi150 – 156Combined sources7
Beta strandi162 – 171Combined sources10
Beta strandi178 – 183Combined sources6
Beta strandi187 – 194Combined sources8
Turni195 – 198Combined sources4
Beta strandi199 – 206Combined sources8
Beta strandi213 – 219Combined sources7
Beta strandi231 – 236Combined sources6
Beta strandi240 – 247Combined sources8
Turni248 – 251Combined sources4
Beta strandi252 – 258Combined sources7
Beta strandi265 – 272Combined sources8
Beta strandi274 – 276Combined sources3
Beta strandi280 – 282Combined sources3
Beta strandi286 – 292Combined sources7
Turni294 – 296Combined sources3
Beta strandi298 – 305Combined sources8
Turni306 – 309Combined sources4
Beta strandi310 – 318Combined sources9
Beta strandi320 – 322Combined sources3
Beta strandi325 – 331Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RI6X-ray2.00A2-331[»]
ProteinModelPortaliP52697.
SMRiP52697.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52697.

Family & Domainsi

Sequence similaritiesi

Belongs to the cycloisomerase 2 family.Curated

Phylogenomic databases

eggNOGiENOG4105HHQ. Bacteria.
COG2706. LUCA.
HOGENOMiHOG000257418.
InParanoidiP52697.
KOiK07404.
OMAiMVFHPNQ.
PhylomeDBiP52697.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_01605. 6P_gluconolactonase. 1 hit.
InterProiIPR022528. 6-phosphogluconolactonase_YbhE.
IPR019405. Lactonase_7-beta_prop.
IPR011045. N2O_reductase_N.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF10282. Lactonase. 1 hit.
[Graphical view]
SUPFAMiSSF50974. SSF50974. 1 hit.

Sequencei

Sequence statusi: Complete.

P52697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQTVYIASP ESQQIHVWNL NHEGALTLTQ VVDVPGQVQP MVVSPDKRYL
60 70 80 90 100
YVGVRPEFRV LAYRIAPDDG ALTFAAESAL PGSPTHISTD HQGQFVFVGS
110 120 130 140 150
YNAGNVSVTR LEDGLPVGVV DVVEGLDGCH SANISPDNRT LWVPALKQDR
160 170 180 190 200
ICLFTVSDDG HLVAQDPAEV TTVEGAGPRH MVFHPNEQYA YCVNELNSSV
210 220 230 240 250
DVWELKDPHG NIECVQTLDM MPENFSDTRW AADIHITPDG RHLYACDRTA
260 270 280 290 300
SLITVFSVSE DGSVLSKEGF QPTETQPRGF NVDHSGKYLI AAGQKSHHIS
310 320 330
VYEIVGEQGL LHEKGRYAVG QGPMWVVVNA H
Length:331
Mass (Da):36,308
Last modified:November 1, 1997 - v2
Checksum:iD731044CFCF31A8F
GO

Sequence cautioni

The sequence U27192 differs from that shown. Reason: Frameshift at position 110.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37 – 52QVQPM…RYLYV → RCSRWWSARTNVISML (PubMed:7665460).CuratedAdd BLAST16
Sequence conflicti319 – 331VGQGP…VVNAH → SGRDQCGWWLTHTKR (PubMed:7665460).CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27192 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC73854.1.
AP009048 Genomic DNA. Translation: BAA35431.1.
PIRiG64812.
RefSeqiNP_415288.1. NC_000913.3.
WP_000815435.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73854; AAC73854; b0767.
BAA35431; BAA35431; BAA35431.
GeneIDi946398.
KEGGiecj:JW0750.
eco:b0767.
PATRICi32116735. VBIEscCol129921_0793.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27192 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC73854.1.
AP009048 Genomic DNA. Translation: BAA35431.1.
PIRiG64812.
RefSeqiNP_415288.1. NC_000913.3.
WP_000815435.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RI6X-ray2.00A2-331[»]
ProteinModelPortaliP52697.
SMRiP52697.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261151. 18 interactors.
IntActiP52697. 5 interactors.
STRINGi511145.b0767.

Proteomic databases

EPDiP52697.
PaxDbiP52697.
PRIDEiP52697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73854; AAC73854; b0767.
BAA35431; BAA35431; BAA35431.
GeneIDi946398.
KEGGiecj:JW0750.
eco:b0767.
PATRICi32116735. VBIEscCol129921_0793.

Organism-specific databases

EchoBASEiEB3020.
EcoGeneiEG13231. pgl.

Phylogenomic databases

eggNOGiENOG4105HHQ. Bacteria.
COG2706. LUCA.
HOGENOMiHOG000257418.
InParanoidiP52697.
KOiK07404.
OMAiMVFHPNQ.
PhylomeDBiP52697.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00409.
BioCyciEcoCyc:6PGLUCONOLACT-MONOMER.
ECOL316407:JW0750-MONOMER.
MetaCyc:6PGLUCONOLACT-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP52697.
PROiP52697.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_01605. 6P_gluconolactonase. 1 hit.
InterProiIPR022528. 6-phosphogluconolactonase_YbhE.
IPR019405. Lactonase_7-beta_prop.
IPR011045. N2O_reductase_N.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF10282. Lactonase. 1 hit.
[Graphical view]
SUPFAMiSSF50974. SSF50974. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei6PGL_ECOLI
AccessioniPrimary (citable) accession number: P52697
Secondary accession number(s): P75760
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.