6-phosphogluconolactonase - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P52697 (6PGL_ECOLI)

Last modified November 3, 2009. Version 73. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    6-phosphogluconolactonase
      Short name=6-P-gluconolactonase
      Short name=Pgl
    EC=3.1.1.31

Gene names

Name:	pgl
Synonyms:	ybhE
Ordered Locus Names:	b0767, JW0750

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Hide | Top

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate. Ref.6
Catalytic activity	6-phospho-D-glucono-1,5-lactone + H₂O = 6-phospho-D-gluconate. HAMAP MF_01605
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3. HAMAP MF_01605
Sequence similarities	Belongs to the cycloisomerase 2 family.
Sequence caution	The sequence U27192 differs from that shown. Reason: Frameshift at position 110.

Hide | Top

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Molecular function	Hydrolase
PTM	Acetylation
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	6-phosphogluconolactonase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 331

331

6-phosphogluconolactonase HAMAP MF_01605

PRO_0000171132

Amino acid modifications

Modified residue

287

N6-acetyllysine Ref.7

Experimental info

Sequence conflict

37 – 52

QVQPM…RYLYV → RCSRWWSARTNVISML Ref.1

Sequence conflict

319 – 331

VGQGP…VVNAH → SGRDQCGWWLTHTKR Ref.1

Secondary structure

331

Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P52697-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: D731044CFCF31A8F
Show »

FASTA

331

36,308

        10         20         30         40         50         60 
MKQTVYIASP ESQQIHVWNL NHEGALTLTQ VVDVPGQVQP MVVSPDKRYL YVGVRPEFRV 

        70         80         90        100        110        120 
LAYRIAPDDG ALTFAAESAL PGSPTHISTD HQGQFVFVGS YNAGNVSVTR LEDGLPVGVV 

       130        140        150        160        170        180 
DVVEGLDGCH SANISPDNRT LWVPALKQDR ICLFTVSDDG HLVAQDPAEV TTVEGAGPRH 

       190        200        210        220        230        240 
MVFHPNEQYA YCVNELNSSV DVWELKDPHG NIECVQTLDM MPENFSDTRW AADIHITPDG 

       250        260        270        280        290        300 
RHLYACDRTA SLITVFSVSE DGSVLSKEGF QPTETQPRGF NVDHSGKYLI AAGQKSHHIS 

       310        320        330 
VYEIVGEQGL LHEKGRYAVG QGPMWVVVNA H

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli." Maupin-Furlow J.A., Rosentel J.K., Lee J.H., Deppenmeier U., Gunsalus R.P., Shanmugam K.T. J. Bacteriol. 177:4851-4856(1995) [PubMed: 7665460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	Rudd K.E. Unpublished observations (MAR-1996) Cited for: IDENTIFICATION.
[6]	"Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase." Thomason L.C., Court D.L., Datta A.R., Khanna R., Rosner J.L. J. Bacteriol. 186:8248-8253(2004) [PubMed: 15576773] [Abstract] Cited for: FUNCTION. Strain: K12 / MG1655 / ATCC 47076.
[7]	"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y. Mol. Cell. Proteomics 8:215-225(2009) [PubMed: 18723842] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-287, MASS SPECTROMETRY.
[8]	"Structure of a putative 7-bladed propeller isomerase." Lima C.D., Kniewel R., Solorzano V., Wu J. Submitted (NOV-2003) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Hide | Top

Cross-references

Sequence databases

U27192 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC73854.1.
AP009048 Genomic DNA. Translation: BAA35431.1.

PIR

G64812.

RefSeq

AP_001398.1.
NP_415288.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RI6	X-ray	2.00	A	2-331	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P52697.

2-D gel databases

2DBase-Ecoli

P52697.

Genome annotation databases

GeneID

946398.

GenomeReviews

Gene locus JW0750 in contig AP009048_GR.
Gene locus b0767 in contig U00096_GR.

KEGG

ecj:JW0750.
eco:b0767.

Organism-specific databases

EchoBASE

EB3020.

EcoGene

EG13231. pgl.

CMR

Search...

Phylogenomic databases

HOGENOM

P52697.

OMA

EGCHSAN.

Enzyme and pathway databases

BioCyc

EcoCyc:6PGLUCONOLACT-MON.
MetaCyc:6PGLUCONOLACT-MON.

Gene expression databases

Genevestigator

P52697.

Family and domain databases

HAMAP

MF_01605.
[Tree]

InterPro

IPR019405. DUF2394.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.

Pfam

PF10282. DUF2394. 1 hit.
[Graphical view]

ProtoNet

Search...

Hide | Top

Entry information

Entry name

6PGL_ECOLI

Accession

Primary (citable) accession number: P52697
Secondary accession number(s): P75760

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	November 3, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top