P52682 (BLAN_SERMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbapenem-hydrolyzing beta-lactamase Sme-1
EC=3.5.2.6

Gene names

Name:	smeA
Synonyms:	bpl-1, bplA

Organism

Serratia marcescens

Taxonomic identifier

615 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can hydrolyze carbapenem compounds.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.1
	Chain	28 – 294	267	Carbapenem-hydrolyzing beta-lactamase Sme-1		PRO_0000017001
Regions
	Region	238 – 240	3	Substrate binding By similarity
Sites
	Active site	73	1	Acyl-ester intermediate By similarity
	Active site	172	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52682 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C33AAD6AA2293094	FASTA	294	32,197
	10 20 30 40 50 60 MSNKVNFKTA SFLFSVCLAL SAFNAHANKS DAAAKQIKKL EEDFDGRIGV FAIDTGSGNT 70 80 90 100 110 120 FGYRSDERFP LCSSFKGFLA AAVLERVQQK KLDINQKVKY ESRDLEYHSP ITTKYKGSGM 130 140 150 160 170 180 TLGDMASAAL QYSDNGATNI IMERFLGGPE GMTKFMRSIG DNEFRLDRWE LELNTAIPGD 190 200 210 220 230 240 KRDTSTPKAV ANSLNKLALG NVLNAKVKAI YQNWLKGNTT GDARIRASVP ADWVVGDKTG 250 260 270 280 290 SCGAIGTAND YAVIWPKNRA PLIVSIYTTR KSKDDKHSDK TIAEASRIAI QAID

References

[1]

"Cloning and sequence analysis of the gene for a carbapenem-hydrolyzing class A beta-lactamase, Sme-1, from Serratia marcescens S6." Naas T., Vandel L., Sougakoff W., Livermore D.M., Nordmann P. Antimicrob. Agents Chemother. 38:1262-1270(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-38. Strain: S6.

[2]

"Characterization of an LysR family protein, SmeR from Serratia marcescens S6, its effect on expression of the carbapenem-hydrolyzing beta-lactamase Sme-1, and comparison of this regulator with other beta-lactamase regulators." Naas T., Livermore D.M., Nordmann P. Antimicrob. Agents Chemother. 39:629-637(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32. Strain: S6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28968 Genomic DNA. Translation: CAA82281.1. Z30237 Genomic DNA. Translation: CAA82944.1.
PIR	S44080.
3D structure databases
ProteinModelPortal	P52682.
SMR	P52682. Positions 28-294.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	P52682.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAN_SERMA

Accession

Primary (citable) accession number: P52682

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program