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Protein

Beta-lactamase

Gene

blaB

Organism
Proteus vulgaris
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against cephalosporins such as cefuroxime and cefotaxime.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75Acyl-ester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Cefuroximase
Gene namesi
Name:blaB
Synonyms:cumA
OrganismiProteus vulgaris
Taxonomic identifieri585 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesMorganellaceaeProteus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000001700830 – 300Beta-lactamaseAdd BLAST271

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

Secondary structure

1300
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi34 – 46Combined sources13
Beta strandi48 – 56Combined sources9
Turni57 – 60Combined sources4
Beta strandi61 – 66Combined sources6
Helixi74 – 77Combined sources4
Helixi78 – 91Combined sources14
Helixi95 – 97Combined sources3
Helixi104 – 106Combined sources3
Helixi114 – 116Combined sources3
Turni118 – 120Combined sources3
Helixi124 – 133Combined sources10
Helixi137 – 147Combined sources11
Helixi150 – 159Combined sources10
Helixi173 – 175Combined sources3
Helixi188 – 199Combined sources12
Beta strandi201 – 204Combined sources4
Helixi206 – 217Combined sources12
Turni223 – 225Combined sources3
Helixi226 – 229Combined sources4
Beta strandi234 – 243Combined sources10
Turni244 – 246Combined sources3
Beta strandi247 – 255Combined sources9
Beta strandi262 – 269Combined sources8
Helixi279 – 294Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HZOX-ray1.75A/B32-300[»]
ProteinModelPortaliP52664.
SMRiP52664.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52664.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni239 – 241Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52664-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMFKTTFRQ TATIAVSLIS LLVSPMLWAN TNNTIEEQLS TLEKYSQGRL
60 70 80 90 100
GVALINTEDN SQITYRGEER FAMASTSKVM AVAAVLKESE KQAGLLDKNI
110 120 130 140 150
TIKKSDLVAY SPITEKHLVT GMSLAQLSAA TLQYSDNTAM NKILDYLGGP
160 170 180 190 200
AKVTQFARSI NDVTYRLDRK EPELNTAIHG DPRDTTSPIA MAKSLQALTL
210 220 230 240 250
GDALGQSQRQ QLVTWLKGNT TGDHSIKAGL PKHWIVGDKT GSGDYGTTND
260 270 280 290 300
IAVIWPKNHA PLILVVYFTQ QEQDAKYRKD IIVKATEIVT KEISNSPQTK
Length:300
Mass (Da):32,992
Last modified:October 1, 1996 - v1
Checksum:i2719AF2464F1CD77
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti13T → A in strain: 5E78-1. 1
Natural varianti23V → A in strain: 5E78-1. 1
Natural varianti30 – 31NT → DN in strain: 5E78-1. 2
Natural varianti40S → N in strain: 5E78-1. 1
Natural varianti58E → K in strain: 5E78-1. 1
Natural varianti88E → A in strain: 5E78-1. 1
Natural varianti119V → T in strain: 5E78-1. 1
Natural varianti123S → T in strain: 5E78-1. 1
Natural varianti126Q → E in strain: 5E78-1. 1
Natural varianti224H → N in strain: 5E78-1. 1
Natural varianti235I → V in strain: 5E78-1. 1
Natural varianti257K → E in strain: 5E78-1. 1
Natural varianti283V → A in strain: 5E78-1. 1
Natural varianti286T → A in strain: 5E78-1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80128 Genomic DNA. Translation: CAA56427.1.
D37831 Genomic DNA. Translation: BAA07084.1.
PIRiS51044.
RefSeqiWP_063851288.1. NG_047908.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80128 Genomic DNA. Translation: CAA56427.1.
D37831 Genomic DNA. Translation: BAA07084.1.
PIRiS51044.
RefSeqiWP_063851288.1. NG_047908.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HZOX-ray1.75A/B32-300[»]
ProteinModelPortaliP52664.
SMRiP52664.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP52664.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLAB_PROVU
AccessioniPrimary (citable) accession number: P52664
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.