Reviewed, UniProtKB/Swiss-Prot P52664 (BLAB_PROVU)

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6
Alternative name(s):
    Cefuroximase

Gene names

Name:	blaB
Synonyms:	cumA

Organism

Proteus vulgaris

Taxonomic identifier

585 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against cephalosporins such as cefuroxime and cefotaxime.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 29

29

Potential

Chain

30 – 300

271

Beta-lactamase

PRO_0000017008

Regions

Region

239 – 241

3

Substrate binding By similarity

Sites

Active site

75

1

Acyl-ester intermediate By similarity

Natural variations

Natural variant

13

1

T → A in strain: 5E78-1.

Natural variant

23

1

V → A in strain: 5E78-1.

Natural variant

30 – 31

2

NT → DN in strain: 5E78-1.

Natural variant

40

1

S → N in strain: 5E78-1.

Natural variant

58

1

E → K in strain: 5E78-1.

Natural variant

88

1

E → A in strain: 5E78-1.

Natural variant

119

1

V → T in strain: 5E78-1.

Natural variant

123

1

S → T in strain: 5E78-1.

Natural variant

126

1

Q → E in strain: 5E78-1.

Natural variant

224

1

H → N in strain: 5E78-1.

Natural variant

235

1

I → V in strain: 5E78-1.

Natural variant

257

1

K → E in strain: 5E78-1.

Natural variant

283

1

V → A in strain: 5E78-1.

Natural variant

286

1

T → A in strain: 5E78-1.

Secondary structure

1

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300

Helix Strand Turn

Details...

Helix

34 – 46

13

Beta strand

48 – 56

9

Turn

57 – 60

4

Beta strand

61 – 66

6

Helix

74 – 77

4

Helix

78 – 91

14

Helix

95 – 97

3

Helix

104 – 106

3

Helix

114 – 116

3

Turn

118 – 120

3

Helix

124 – 133

10

Helix

137 – 147

11

Helix

150 – 159

10

Helix

173 – 175

3

Helix

188 – 199

12

Beta strand

201 – 204

4

Helix

206 – 217

12

Turn

223 – 225

3

Helix

226 – 229

4

Beta strand

234 – 243

10

Turn

244 – 246

3

Beta strand

247 – 255

9

Beta strand

262 – 269

8

Helix

279 – 294

16

Sequences

	Sequence		Length	Mass (Da)
	P52664-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2719AF2464F1CD77	FASTA	300	32,992
	10 20 30 40 50 60 MTMFKTTFRQ TATIAVSLIS LLVSPMLWAN TNNTIEEQLS TLEKYSQGRL GVALINTEDN 70 80 90 100 110 120 SQITYRGEER FAMASTSKVM AVAAVLKESE KQAGLLDKNI TIKKSDLVAY SPITEKHLVT 130 140 150 160 170 180 GMSLAQLSAA TLQYSDNTAM NKILDYLGGP AKVTQFARSI NDVTYRLDRK EPELNTAIHG 190 200 210 220 230 240 DPRDTTSPIA MAKSLQALTL GDALGQSQRQ QLVTWLKGNT TGDHSIKAGL PKHWIVGDKT 250 260 270 280 290 300 GSGDYGTTND IAVIWPKNHA PLILVVYFTQ QEQDAKYRKD IIVKATEIVT KEISNSPQTK

References

[1]	"A common system controls the induction of very different genes. The class-A beta-lactamase of Proteus vulgaris and the enterobacterial class-C beta-lactamase." Datz M., Joris B., Azab E.A., Galleni M., van Beeumen J., Frere J.-M., Martin H.H. Eur. J. Biochem. 226:149-157(1994) [PubMed: 7957242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B317.
[2]	Okuguchi M., Komai T., Makajima N., Eguchi H., Kuboki A., Ito T., Meguro M., Nakata T., Sugimoto K. Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 5E78-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X80128 Genomic DNA. Translation: CAA56427.1.
D37831 Genomic DNA. Translation: BAA07084.1.

PIR

S51044.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HZO	X-ray	1.75	A/B	32-300	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.5.2.6. 641.

Family and domain databases

InterPro

IPR001466. Beta_lactamase-related.
IPR000871. Beta_lactamase_A/D.
[Graphical view]

Pfam

PF00144. Beta-lactamase. 1 hit.
[Graphical view]

PRINTS

PR00118. BLACTAMASEA.

PROSITE

PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BLAB_PROVU

Accession

Primary (citable) accession number: P52664

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)