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P52664

- BLAB_PROVU

UniProt

P52664 - BLAB_PROVU

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Protein
Beta-lactamase
Gene
blaB, cumA
Organism
Proteus vulgaris
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against cephalosporins such as cefuroxime and cefotaxime.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Acyl-ester intermediate By similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Cefuroximase
Gene namesi
Name:blaB
Synonyms:cumA
OrganismiProteus vulgaris
Taxonomic identifieri585 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed prediction
Add
BLAST
Chaini30 – 300271Beta-lactamase
PRO_0000017008Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi34 – 4613
Beta strandi48 – 569
Turni57 – 604
Beta strandi61 – 666
Helixi74 – 774
Helixi78 – 9114
Helixi95 – 973
Helixi104 – 1063
Helixi114 – 1163
Turni118 – 1203
Helixi124 – 13310
Helixi137 – 14711
Helixi150 – 15910
Helixi173 – 1753
Helixi188 – 19912
Beta strandi201 – 2044
Helixi206 – 21712
Turni223 – 2253
Helixi226 – 2294
Beta strandi234 – 24310
Turni244 – 2463
Beta strandi247 – 2559
Beta strandi262 – 2698
Helixi279 – 29416

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HZOX-ray1.75A/B32-300[»]
ProteinModelPortaliP52664.
SMRiP52664. Positions 33-296.

Miscellaneous databases

EvolutionaryTraceiP52664.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni239 – 2413Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52664-1 [UniParc]FASTAAdd to Basket

« Hide

MTMFKTTFRQ TATIAVSLIS LLVSPMLWAN TNNTIEEQLS TLEKYSQGRL    50
GVALINTEDN SQITYRGEER FAMASTSKVM AVAAVLKESE KQAGLLDKNI 100
TIKKSDLVAY SPITEKHLVT GMSLAQLSAA TLQYSDNTAM NKILDYLGGP 150
AKVTQFARSI NDVTYRLDRK EPELNTAIHG DPRDTTSPIA MAKSLQALTL 200
GDALGQSQRQ QLVTWLKGNT TGDHSIKAGL PKHWIVGDKT GSGDYGTTND 250
IAVIWPKNHA PLILVVYFTQ QEQDAKYRKD IIVKATEIVT KEISNSPQTK 300
Length:300
Mass (Da):32,992
Last modified:October 1, 1996 - v1
Checksum:i2719AF2464F1CD77
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti13 – 131T → A in strain: 5E78-1.
Natural varianti23 – 231V → A in strain: 5E78-1.
Natural varianti30 – 312NT → DN in strain: 5E78-1.
Natural varianti40 – 401S → N in strain: 5E78-1.
Natural varianti58 – 581E → K in strain: 5E78-1.
Natural varianti88 – 881E → A in strain: 5E78-1.
Natural varianti119 – 1191V → T in strain: 5E78-1.
Natural varianti123 – 1231S → T in strain: 5E78-1.
Natural varianti126 – 1261Q → E in strain: 5E78-1.
Natural varianti224 – 2241H → N in strain: 5E78-1.
Natural varianti235 – 2351I → V in strain: 5E78-1.
Natural varianti257 – 2571K → E in strain: 5E78-1.
Natural varianti283 – 2831V → A in strain: 5E78-1.
Natural varianti286 – 2861T → A in strain: 5E78-1.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80128 Genomic DNA. Translation: CAA56427.1.
D37831 Genomic DNA. Translation: BAA07084.1.
PIRiS51044.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80128 Genomic DNA. Translation: CAA56427.1 .
D37831 Genomic DNA. Translation: BAA07084.1 .
PIRi S51044.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HZO X-ray 1.75 A/B 32-300 [» ]
ProteinModelPortali P52664.
SMRi P52664. Positions 33-296.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P52664.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A common system controls the induction of very different genes. The class-A beta-lactamase of Proteus vulgaris and the enterobacterial class-C beta-lactamase."
    Datz M., Joris B., Azab E.A., Galleni M., van Beeumen J., Frere J.-M., Martin H.H.
    Eur. J. Biochem. 226:149-157(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B317.
  2. Okuguchi M., Komai T., Makajima N., Eguchi H., Kuboki A., Ito T., Meguro M., Nakata T., Sugimoto K.
    Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 5E78-1.

Entry informationi

Entry nameiBLAB_PROVU
AccessioniPrimary (citable) accession number: P52664
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 16, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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