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P52664

- BLAB_PROVU

UniProt

P52664 - BLAB_PROVU

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Protein

Beta-lactamase

Gene

blaB

Organism
Proteus vulgaris
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against cephalosporins such as cefuroxime and cefotaxime.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Acyl-ester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Cefuroximase
Gene namesi
Name:blaB
Synonyms:cumA
OrganismiProteus vulgaris
Taxonomic identifieri585 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Chaini30 – 300271Beta-lactamasePRO_0000017008Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

Secondary structure

1
300
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi34 – 4613Combined sources
Beta strandi48 – 569Combined sources
Turni57 – 604Combined sources
Beta strandi61 – 666Combined sources
Helixi74 – 774Combined sources
Helixi78 – 9114Combined sources
Helixi95 – 973Combined sources
Helixi104 – 1063Combined sources
Helixi114 – 1163Combined sources
Turni118 – 1203Combined sources
Helixi124 – 13310Combined sources
Helixi137 – 14711Combined sources
Helixi150 – 15910Combined sources
Helixi173 – 1753Combined sources
Helixi188 – 19912Combined sources
Beta strandi201 – 2044Combined sources
Helixi206 – 21712Combined sources
Turni223 – 2253Combined sources
Helixi226 – 2294Combined sources
Beta strandi234 – 24310Combined sources
Turni244 – 2463Combined sources
Beta strandi247 – 2559Combined sources
Beta strandi262 – 2698Combined sources
Helixi279 – 29416Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HZOX-ray1.75A/B32-300[»]
ProteinModelPortaliP52664.
SMRiP52664. Positions 33-296.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52664.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni239 – 2413Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52664-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTMFKTTFRQ TATIAVSLIS LLVSPMLWAN TNNTIEEQLS TLEKYSQGRL
60 70 80 90 100
GVALINTEDN SQITYRGEER FAMASTSKVM AVAAVLKESE KQAGLLDKNI
110 120 130 140 150
TIKKSDLVAY SPITEKHLVT GMSLAQLSAA TLQYSDNTAM NKILDYLGGP
160 170 180 190 200
AKVTQFARSI NDVTYRLDRK EPELNTAIHG DPRDTTSPIA MAKSLQALTL
210 220 230 240 250
GDALGQSQRQ QLVTWLKGNT TGDHSIKAGL PKHWIVGDKT GSGDYGTTND
260 270 280 290 300
IAVIWPKNHA PLILVVYFTQ QEQDAKYRKD IIVKATEIVT KEISNSPQTK
Length:300
Mass (Da):32,992
Last modified:October 1, 1996 - v1
Checksum:i2719AF2464F1CD77
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti13 – 131T → A in strain: 5E78-1.
Natural varianti23 – 231V → A in strain: 5E78-1.
Natural varianti30 – 312NT → DN in strain: 5E78-1.
Natural varianti40 – 401S → N in strain: 5E78-1.
Natural varianti58 – 581E → K in strain: 5E78-1.
Natural varianti88 – 881E → A in strain: 5E78-1.
Natural varianti119 – 1191V → T in strain: 5E78-1.
Natural varianti123 – 1231S → T in strain: 5E78-1.
Natural varianti126 – 1261Q → E in strain: 5E78-1.
Natural varianti224 – 2241H → N in strain: 5E78-1.
Natural varianti235 – 2351I → V in strain: 5E78-1.
Natural varianti257 – 2571K → E in strain: 5E78-1.
Natural varianti283 – 2831V → A in strain: 5E78-1.
Natural varianti286 – 2861T → A in strain: 5E78-1.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80128 Genomic DNA. Translation: CAA56427.1.
D37831 Genomic DNA. Translation: BAA07084.1.
PIRiS51044.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80128 Genomic DNA. Translation: CAA56427.1 .
D37831 Genomic DNA. Translation: BAA07084.1 .
PIRi S51044.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HZO X-ray 1.75 A/B 32-300 [» ]
ProteinModelPortali P52664.
SMRi P52664. Positions 33-296.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P52664.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A common system controls the induction of very different genes. The class-A beta-lactamase of Proteus vulgaris and the enterobacterial class-C beta-lactamase."
    Datz M., Joris B., Azab E.A., Galleni M., van Beeumen J., Frere J.-M., Martin H.H.
    Eur. J. Biochem. 226:149-157(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B317.
  2. Okuguchi M., Komai T., Makajima N., Eguchi H., Kuboki A., Ito T., Meguro M., Nakata T., Sugimoto K.
    Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 5E78-1.

Entry informationi

Entry nameiBLAB_PROVU
AccessioniPrimary (citable) accession number: P52664
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3