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P52664

- BLAB_PROVU

UniProt

P52664 - BLAB_PROVU

Protein

Beta-lactamase

Gene

blaB

Organism
Proteus vulgaris
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against cephalosporins such as cefuroxime and cefotaxime.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei75 – 751Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase (EC:3.5.2.6)
    Alternative name(s):
    Cefuroximase
    Gene namesi
    Name:blaB
    Synonyms:cumA
    OrganismiProteus vulgaris
    Taxonomic identifieri585 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 300271Beta-lactamasePRO_0000017008Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    Secondary structure

    1
    300
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi34 – 4613
    Beta strandi48 – 569
    Turni57 – 604
    Beta strandi61 – 666
    Helixi74 – 774
    Helixi78 – 9114
    Helixi95 – 973
    Helixi104 – 1063
    Helixi114 – 1163
    Turni118 – 1203
    Helixi124 – 13310
    Helixi137 – 14711
    Helixi150 – 15910
    Helixi173 – 1753
    Helixi188 – 19912
    Beta strandi201 – 2044
    Helixi206 – 21712
    Turni223 – 2253
    Helixi226 – 2294
    Beta strandi234 – 24310
    Turni244 – 2463
    Beta strandi247 – 2559
    Beta strandi262 – 2698
    Helixi279 – 29416

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HZOX-ray1.75A/B32-300[»]
    ProteinModelPortaliP52664.
    SMRiP52664. Positions 33-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP52664.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni239 – 2413Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52664-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTMFKTTFRQ TATIAVSLIS LLVSPMLWAN TNNTIEEQLS TLEKYSQGRL    50
    GVALINTEDN SQITYRGEER FAMASTSKVM AVAAVLKESE KQAGLLDKNI 100
    TIKKSDLVAY SPITEKHLVT GMSLAQLSAA TLQYSDNTAM NKILDYLGGP 150
    AKVTQFARSI NDVTYRLDRK EPELNTAIHG DPRDTTSPIA MAKSLQALTL 200
    GDALGQSQRQ QLVTWLKGNT TGDHSIKAGL PKHWIVGDKT GSGDYGTTND 250
    IAVIWPKNHA PLILVVYFTQ QEQDAKYRKD IIVKATEIVT KEISNSPQTK 300
    Length:300
    Mass (Da):32,992
    Last modified:October 1, 1996 - v1
    Checksum:i2719AF2464F1CD77
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti13 – 131T → A in strain: 5E78-1.
    Natural varianti23 – 231V → A in strain: 5E78-1.
    Natural varianti30 – 312NT → DN in strain: 5E78-1.
    Natural varianti40 – 401S → N in strain: 5E78-1.
    Natural varianti58 – 581E → K in strain: 5E78-1.
    Natural varianti88 – 881E → A in strain: 5E78-1.
    Natural varianti119 – 1191V → T in strain: 5E78-1.
    Natural varianti123 – 1231S → T in strain: 5E78-1.
    Natural varianti126 – 1261Q → E in strain: 5E78-1.
    Natural varianti224 – 2241H → N in strain: 5E78-1.
    Natural varianti235 – 2351I → V in strain: 5E78-1.
    Natural varianti257 – 2571K → E in strain: 5E78-1.
    Natural varianti283 – 2831V → A in strain: 5E78-1.
    Natural varianti286 – 2861T → A in strain: 5E78-1.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80128 Genomic DNA. Translation: CAA56427.1.
    D37831 Genomic DNA. Translation: BAA07084.1.
    PIRiS51044.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80128 Genomic DNA. Translation: CAA56427.1 .
    D37831 Genomic DNA. Translation: BAA07084.1 .
    PIRi S51044.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HZO X-ray 1.75 A/B 32-300 [» ]
    ProteinModelPortali P52664.
    SMRi P52664. Positions 33-296.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P52664.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A common system controls the induction of very different genes. The class-A beta-lactamase of Proteus vulgaris and the enterobacterial class-C beta-lactamase."
      Datz M., Joris B., Azab E.A., Galleni M., van Beeumen J., Frere J.-M., Martin H.H.
      Eur. J. Biochem. 226:149-157(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: B317.
    2. Okuguchi M., Komai T., Makajima N., Eguchi H., Kuboki A., Ito T., Meguro M., Nakata T., Sugimoto K.
      Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 5E78-1.

    Entry informationi

    Entry nameiBLAB_PROVU
    AccessioniPrimary (citable) accession number: P52664
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3