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P52663

- BLAN_ENTCL

UniProt

P52663 - BLAN_ENTCL

Protein

Imipenem-hydrolyzing beta-lactamase

Gene

nmcA

Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Hydrolyzes carbapenems such as imipenem, which are extended-spectrum beta-lactam antibiotics.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei71 – 711Acyl-ester intermediate

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    SABIO-RKP52663.

    Protein family/group databases

    MEROPSiS11.A01.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Imipenem-hydrolyzing beta-lactamase (EC:3.5.2.6)
    Alternative name(s):
    Carbapenemase
    NMC-A
    Gene namesi
    Name:nmcA
    OrganismiEnterobacter cloacae
    Taxonomic identifieri550 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 27271 PublicationAdd
    BLAST
    Chaini28 – 292265Imipenem-hydrolyzing beta-lactamasePRO_0000017000Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi70 ↔ 240

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Structurei

    Secondary structure

    1
    292
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi32 – 4211
    Beta strandi44 – 529
    Turni53 – 553
    Beta strandi58 – 625
    Helixi70 – 734
    Helixi74 – 8613
    Helixi108 – 1125
    Turni113 – 1164
    Helixi120 – 12910
    Helixi133 – 14210
    Helixi146 – 15611
    Helixi170 – 1723
    Helixi185 – 19713
    Beta strandi198 – 2014
    Helixi203 – 21412
    Turni220 – 2223
    Helixi223 – 2264
    Beta strandi231 – 2399
    Beta strandi246 – 2538
    Beta strandi260 – 2678
    Helixi277 – 28913

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BUEX-ray1.64A28-292[»]
    1BULX-ray1.89A28-292[»]
    ProteinModelPortaliP52663.
    SMRiP52663. Positions 28-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP52663.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni236 – 2383Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52663-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLNVKQSRI AILFSSCLIS ISFFSQANTK GIDEIKNLET DFNGRIGVYA    50
    LDTGSGKSFS YRANERFPLC SSFKGFLAAA VLKGSQDNRL NLNQIVNYNT 100
    RSLEFHSPIT TKYKDNGMSL GDMAAAALQY SDNGATNIIL ERYIGGPEGM 150
    TKFMRSIGDE DFRLDRWELD LNTAIPGDER DTSTPAAVAK SLKTLALGNI 200
    LSEHEKETYQ TWLKGNTTGA ARIRASVPSD WVVGDKTGSC GAYGTANDYA 250
    VVWPKNRAPL IISVYTTKNE KEAKHEDKVI AEASRIAIDN LK 292
    Length:292
    Mass (Da):32,075
    Last modified:October 1, 1996 - v1
    Checksum:i4D33FD8669998F1B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21956 Genomic DNA. Translation: CAA79967.1.
    PIRiS35915.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21956 Genomic DNA. Translation: CAA79967.1 .
    PIRi S35915.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BUE X-ray 1.64 A 28-292 [» ]
    1BUL X-ray 1.89 A 28-292 [» ]
    ProteinModelPortali P52663.
    SMRi P52663. Positions 28-292.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P52663.
    ChEMBLi CHEMBL4314.

    Protein family/group databases

    MEROPSi S11.A01.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P52663.

    Miscellaneous databases

    EvolutionaryTracei P52663.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a carbapenem-hydrolyzing class A beta-lactamase from Enterobacter cloacae and of its LysR-type regulatory protein."
      Naas T., Nordmann P.
      Proc. Natl. Acad. Sci. U.S.A. 91:7693-7697(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-34.
      Strain: NOR-1.
    2. "Inhibition of the broad spectrum nonmetallocarbapenamase of class A (NMC-A) beta-lactamase from Enterobacter cloacae by monocyclic beta-lactams."
      Mourey L., Kotra L.P., Bellettini J., Bulychev A., O'Brien M., Miller M.J., Mobashery S., Samama J.-P.
      J. Biol. Chem. 274:25260-25265(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS).
      Strain: NOR-1.

    Entry informationi

    Entry nameiBLAN_ENTCL
    AccessioniPrimary (citable) accession number: P52663
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3