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Protein

Imipenem-hydrolyzing beta-lactamase

Gene

nmcA

Organism
Enterobacter cloacae
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes carbapenems such as imipenem, which are extended-spectrum beta-lactam antibiotics.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71Acyl-ester intermediate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKP52663.

Names & Taxonomyi

Protein namesi
Recommended name:
Imipenem-hydrolyzing beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Carbapenemase
NMC-A
Gene namesi
Name:nmcA
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4314.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000001700028 – 292Imipenem-hydrolyzing beta-lactamaseAdd BLAST265

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 240

Keywords - PTMi

Disulfide bond

Interactioni

Chemistry databases

BindingDBiP52663.

Structurei

Secondary structure

1292
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 42Combined sources11
Beta strandi44 – 52Combined sources9
Turni53 – 55Combined sources3
Beta strandi58 – 62Combined sources5
Helixi70 – 73Combined sources4
Helixi74 – 86Combined sources13
Helixi108 – 112Combined sources5
Turni113 – 116Combined sources4
Helixi120 – 129Combined sources10
Helixi133 – 142Combined sources10
Helixi146 – 156Combined sources11
Helixi170 – 172Combined sources3
Helixi185 – 197Combined sources13
Beta strandi198 – 201Combined sources4
Helixi203 – 214Combined sources12
Turni220 – 222Combined sources3
Helixi223 – 226Combined sources4
Beta strandi231 – 239Combined sources9
Beta strandi246 – 253Combined sources8
Beta strandi260 – 267Combined sources8
Helixi277 – 289Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BUEX-ray1.64A28-292[»]
1BULX-ray1.89A28-292[»]
ProteinModelPortaliP52663.
SMRiP52663.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52663.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 238Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52663-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLNVKQSRI AILFSSCLIS ISFFSQANTK GIDEIKNLET DFNGRIGVYA
60 70 80 90 100
LDTGSGKSFS YRANERFPLC SSFKGFLAAA VLKGSQDNRL NLNQIVNYNT
110 120 130 140 150
RSLEFHSPIT TKYKDNGMSL GDMAAAALQY SDNGATNIIL ERYIGGPEGM
160 170 180 190 200
TKFMRSIGDE DFRLDRWELD LNTAIPGDER DTSTPAAVAK SLKTLALGNI
210 220 230 240 250
LSEHEKETYQ TWLKGNTTGA ARIRASVPSD WVVGDKTGSC GAYGTANDYA
260 270 280 290
VVWPKNRAPL IISVYTTKNE KEAKHEDKVI AEASRIAIDN LK
Length:292
Mass (Da):32,075
Last modified:October 1, 1996 - v1
Checksum:i4D33FD8669998F1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21956 Genomic DNA. Translation: CAA79967.1.
PIRiS35915.
RefSeqiWP_050737109.1. NZ_JYMF01000060.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21956 Genomic DNA. Translation: CAA79967.1.
PIRiS35915.
RefSeqiWP_050737109.1. NZ_JYMF01000060.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BUEX-ray1.64A28-292[»]
1BULX-ray1.89A28-292[»]
ProteinModelPortaliP52663.
SMRiP52663.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP52663.
ChEMBLiCHEMBL4314.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP52663.

Miscellaneous databases

EvolutionaryTraceiP52663.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBLAN_ENTCL
AccessioniPrimary (citable) accession number: P52663
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.