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P52663

- BLAN_ENTCL

UniProt

P52663 - BLAN_ENTCL

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Protein

Imipenem-hydrolyzing beta-lactamase

Gene

nmcA

Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes carbapenems such as imipenem, which are extended-spectrum beta-lactam antibiotics.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Acyl-ester intermediate

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKP52663.

Protein family/group databases

MEROPSiS11.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
Imipenem-hydrolyzing beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Carbapenemase
NMC-A
Gene namesi
Name:nmcA
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 292265Imipenem-hydrolyzing beta-lactamasePRO_0000017000Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 240

Keywords - PTMi

Disulfide bond

Interactioni

Structurei

Secondary structure

1
292
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi32 – 4211Combined sources
Beta strandi44 – 529Combined sources
Turni53 – 553Combined sources
Beta strandi58 – 625Combined sources
Helixi70 – 734Combined sources
Helixi74 – 8613Combined sources
Helixi108 – 1125Combined sources
Turni113 – 1164Combined sources
Helixi120 – 12910Combined sources
Helixi133 – 14210Combined sources
Helixi146 – 15611Combined sources
Helixi170 – 1723Combined sources
Helixi185 – 19713Combined sources
Beta strandi198 – 2014Combined sources
Helixi203 – 21412Combined sources
Turni220 – 2223Combined sources
Helixi223 – 2264Combined sources
Beta strandi231 – 2399Combined sources
Beta strandi246 – 2538Combined sources
Beta strandi260 – 2678Combined sources
Helixi277 – 28913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BUEX-ray1.64A28-292[»]
1BULX-ray1.89A28-292[»]
ProteinModelPortaliP52663.
SMRiP52663. Positions 28-292.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52663.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni236 – 2383Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52663-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLNVKQSRI AILFSSCLIS ISFFSQANTK GIDEIKNLET DFNGRIGVYA
60 70 80 90 100
LDTGSGKSFS YRANERFPLC SSFKGFLAAA VLKGSQDNRL NLNQIVNYNT
110 120 130 140 150
RSLEFHSPIT TKYKDNGMSL GDMAAAALQY SDNGATNIIL ERYIGGPEGM
160 170 180 190 200
TKFMRSIGDE DFRLDRWELD LNTAIPGDER DTSTPAAVAK SLKTLALGNI
210 220 230 240 250
LSEHEKETYQ TWLKGNTTGA ARIRASVPSD WVVGDKTGSC GAYGTANDYA
260 270 280 290
VVWPKNRAPL IISVYTTKNE KEAKHEDKVI AEASRIAIDN LK
Length:292
Mass (Da):32,075
Last modified:October 1, 1996 - v1
Checksum:i4D33FD8669998F1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21956 Genomic DNA. Translation: CAA79967.1.
PIRiS35915.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21956 Genomic DNA. Translation: CAA79967.1 .
PIRi S35915.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BUE X-ray 1.64 A 28-292 [» ]
1BUL X-ray 1.89 A 28-292 [» ]
ProteinModelPortali P52663.
SMRi P52663. Positions 28-292.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P52663.
ChEMBLi CHEMBL4314.

Protein family/group databases

MEROPSi S11.A01.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P52663.

Miscellaneous databases

EvolutionaryTracei P52663.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Analysis of a carbapenem-hydrolyzing class A beta-lactamase from Enterobacter cloacae and of its LysR-type regulatory protein."
    Naas T., Nordmann P.
    Proc. Natl. Acad. Sci. U.S.A. 91:7693-7697(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-34.
    Strain: NOR-1.
  2. "Inhibition of the broad spectrum nonmetallocarbapenamase of class A (NMC-A) beta-lactamase from Enterobacter cloacae by monocyclic beta-lactams."
    Mourey L., Kotra L.P., Bellettini J., Bulychev A., O'Brien M., Miller M.J., Mobashery S., Samama J.-P.
    J. Biol. Chem. 274:25260-25265(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS).
    Strain: NOR-1.

Entry informationi

Entry nameiBLAN_ENTCL
AccessioniPrimary (citable) accession number: P52663
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3