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P52657

- T2AG_HUMAN

UniProt

P52657 - T2AG_HUMAN

Protein

Transcription initiation factor IIA subunit 2

Gene

GTF2A2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein heterodimerization activity Source: BHF-UCL
    3. protein homodimerization activity Source: BHF-UCL
    4. TBP-class protein binding Source: BHF-UCL
    5. transcription coactivator activity Source: BHF-UCL
    6. transcription factor binding Source: BHF-UCL

    GO - Biological processi

    1. gene expression Source: Reactome
    2. positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
    3. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    4. RNA polymerase II transcriptional preinitiation complex assembly Source: BHF-UCL
    5. transcription elongation from RNA polymerase II promoter Source: Reactome
    6. transcription from RNA polymerase II promoter Source: BHF-UCL
    7. transcription initiation from RNA polymerase II promoter Source: Reactome
    8. viral process Source: Reactome

    Keywords - Biological processi

    Host-virus interaction, Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_6233. Transcription of the HIV genome.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6332. HIV Transcription Initiation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor IIA subunit 2
    Alternative name(s):
    General transcription factor IIA subunit 2
    TFIIA p12 subunit
    Short name:
    TFIIA-12
    Short name:
    TFIIAS
    Transcription initiation factor IIA gamma chain
    Short name:
    TFIIA-gamma
    Gene namesi
    Name:GTF2A2
    Synonyms:TF2A2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:4647. GTF2A2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: HPA
    2. nucleoplasm Source: Reactome
    3. nucleus Source: HPA
    4. transcription factor TFIIA complex Source: BHF-UCL

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29034.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 109109Transcription initiation factor IIA subunit 2PRO_0000194042Add
    BLAST

    Proteomic databases

    MaxQBiP52657.
    PaxDbiP52657.
    PRIDEiP52657.

    PTM databases

    PhosphoSiteiP52657.

    Expressioni

    Gene expression databases

    ArrayExpressiP52657.
    BgeeiP52657.
    CleanExiHS_GTF2A2.
    GenevestigatoriP52657.

    Organism-specific databases

    HPAiHPA056239.

    Interactioni

    Subunit structurei

    TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12). Interacts with NCOA6 general coactivator By similarity. TFIIA forms a complex with TBP. Interacts with SV40 Large T antigen.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TBPP202262EBI-1045262,EBI-355371

    Protein-protein interaction databases

    BioGridi109213. 9 interactions.
    IntActiP52657. 2 interactions.
    STRINGi9606.ENSP00000379372.

    Structurei

    Secondary structure

    1
    109
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 74
    Helixi10 – 2415
    Helixi30 – 5021
    Beta strandi55 – 6814
    Beta strandi71 – 8212
    Beta strandi87 – 9812

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NVPX-ray2.10D2-109[»]
    ProteinModelPortaliP52657.
    SMRiP52657. Positions 3-99.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP52657.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TFIIA subunit 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG5123.
    HOGENOMiHOG000167251.
    HOVERGENiHBG000826.
    InParanoidiP52657.
    KOiK03123.
    OMAiCKNGDAI.
    OrthoDBiEOG7RFTKP.
    PhylomeDBiP52657.
    TreeFamiTF315131.

    Family and domain databases

    Gene3Di1.10.287.190. 1 hit.
    2.30.18.10. 1 hit.
    InterProiIPR009083. TFIIA_a-hlx.
    IPR009088. TFIIA_b-brl.
    IPR003194. TFIIA_gsu.
    IPR015871. TFIIA_gsu_C.
    IPR015872. TFIIA_gsu_N.
    [Graphical view]
    PANTHERiPTHR10966. PTHR10966. 1 hit.
    PfamiPF02751. TFIIA_gamma_C. 1 hit.
    PF02268. TFIIA_gamma_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF009415. Hum_TFIIA_gamma. 1 hit.
    ProDomiPD009224. TFIIA_gsu_N. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF47396. SSF47396. 1 hit.
    SSF50784. SSF50784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P52657-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYQLYRNTT LGNSLQESLD ELIQSQQITP QLALQVLLQF DKAINAALAQ    50
    RVRNRVNFRG SLNTYRFCDN VWTFVLNDVE FREVTELIKV DKVKIVACDG 100
    KNTGSNTTE 109
    Length:109
    Mass (Da):12,457
    Last modified:October 1, 1996 - v1
    Checksum:i938E7DC8B9982AE8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81713 mRNA. Translation: CAA57357.1.
    U14193 mRNA. Translation: AAA64951.1.
    U21242 mRNA. Translation: AAB58247.1.
    BT007362 mRNA. Translation: AAP36026.1.
    CR542192 mRNA. Translation: CAG46989.1.
    AC092755 Genomic DNA. No translation available.
    CH471082 Genomic DNA. Translation: EAW77573.1.
    BC000287 mRNA. Translation: AAH00287.1.
    BC001919 mRNA. Translation: AAH01919.1.
    CCDSiCCDS10173.1.
    PIRiI38952.
    RefSeqiNP_004483.1. NM_004492.2.
    XP_005254381.1. XM_005254324.1.
    XP_005254382.1. XM_005254325.1.
    UniGeneiHs.512934.

    Genome annotation databases

    EnsembliENST00000396060; ENSP00000379372; ENSG00000140307.
    ENST00000396061; ENSP00000379373; ENSG00000140307.
    ENST00000396063; ENSP00000379375; ENSG00000140307.
    GeneIDi2958.
    KEGGihsa:2958.
    UCSCiuc002agg.3. human.

    Polymorphism databases

    DMDMi1729806.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81713 mRNA. Translation: CAA57357.1 .
    U14193 mRNA. Translation: AAA64951.1 .
    U21242 mRNA. Translation: AAB58247.1 .
    BT007362 mRNA. Translation: AAP36026.1 .
    CR542192 mRNA. Translation: CAG46989.1 .
    AC092755 Genomic DNA. No translation available.
    CH471082 Genomic DNA. Translation: EAW77573.1 .
    BC000287 mRNA. Translation: AAH00287.1 .
    BC001919 mRNA. Translation: AAH01919.1 .
    CCDSi CCDS10173.1.
    PIRi I38952.
    RefSeqi NP_004483.1. NM_004492.2.
    XP_005254381.1. XM_005254324.1.
    XP_005254382.1. XM_005254325.1.
    UniGenei Hs.512934.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NVP X-ray 2.10 D 2-109 [» ]
    ProteinModelPortali P52657.
    SMRi P52657. Positions 3-99.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109213. 9 interactions.
    IntActi P52657. 2 interactions.
    STRINGi 9606.ENSP00000379372.

    Chemistry

    BindingDBi P52657.

    PTM databases

    PhosphoSitei P52657.

    Polymorphism databases

    DMDMi 1729806.

    Proteomic databases

    MaxQBi P52657.
    PaxDbi P52657.
    PRIDEi P52657.

    Protocols and materials databases

    DNASUi 2958.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000396060 ; ENSP00000379372 ; ENSG00000140307 .
    ENST00000396061 ; ENSP00000379373 ; ENSG00000140307 .
    ENST00000396063 ; ENSP00000379375 ; ENSG00000140307 .
    GeneIDi 2958.
    KEGGi hsa:2958.
    UCSCi uc002agg.3. human.

    Organism-specific databases

    CTDi 2958.
    GeneCardsi GC15M059930.
    HGNCi HGNC:4647. GTF2A2.
    HPAi HPA056239.
    MIMi 600519. gene.
    neXtProti NX_P52657.
    PharmGKBi PA29034.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5123.
    HOGENOMi HOG000167251.
    HOVERGENi HBG000826.
    InParanoidi P52657.
    KOi K03123.
    OMAi CKNGDAI.
    OrthoDBi EOG7RFTKP.
    PhylomeDBi P52657.
    TreeFami TF315131.

    Enzyme and pathway databases

    Reactomei REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_6233. Transcription of the HIV genome.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6332. HIV Transcription Initiation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

    Miscellaneous databases

    EvolutionaryTracei P52657.
    GenomeRNAii 2958.
    NextBioi 11726.
    PROi P52657.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P52657.
    Bgeei P52657.
    CleanExi HS_GTF2A2.
    Genevestigatori P52657.

    Family and domain databases

    Gene3Di 1.10.287.190. 1 hit.
    2.30.18.10. 1 hit.
    InterProi IPR009083. TFIIA_a-hlx.
    IPR009088. TFIIA_b-brl.
    IPR003194. TFIIA_gsu.
    IPR015871. TFIIA_gsu_C.
    IPR015872. TFIIA_gsu_N.
    [Graphical view ]
    PANTHERi PTHR10966. PTHR10966. 1 hit.
    Pfami PF02751. TFIIA_gamma_C. 1 hit.
    PF02268. TFIIA_gamma_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF009415. Hum_TFIIA_gamma. 1 hit.
    ProDomi PD009224. TFIIA_gsu_N. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF47396. SSF47396. 1 hit.
    SSF50784. SSF50784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription."
      Sun X., Ma D., Sheldon M., Yeung K., Reinberg D.
      Genes Dev. 8:2336-2348(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription."
      Ozer J., Moore P.A., Bolden A.H., Lee A., Rosen C.A., Lieberman P.M.
      Genes Dev. 8:2324-2335(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription."
      Dejong J., Bernstein R., Roeder R.G.
      Proc. Natl. Acad. Sci. U.S.A. 92:3313-3317(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Analysis of the DNA sequence and duplication history of human chromosome 15."
      Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
      , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
      Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    9. "Simian virus 40 large T antigen stabilizes the TATA-binding protein-TFIIA complex on the TATA element."
      Damania B., Lieberman P., Alwine J.C.
      Mol. Cell. Biol. 18:3926-3935(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SV40 LARGE T ANTIGEN.
    10. "A nuclear factor ASC-2, as a cancer-amplified transcriptional coactivator essential for ligand-dependent transactivation by nuclear receptors in vivo."
      Lee S.-K., Anzick S.L., Choi J.-E., Bubendorf L., Guan X.-Y., Jung Y.-K., Kallioniemi O.-P., Kononen J., Trent J.M., Azorsa D., Jhun B.-H., Cheong J.H., Lee Y.C., Meltzer P.S., Lee J.W.
      J. Biol. Chem. 274:34283-34293(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NCOA6.
    11. "TAC, a TBP-sans-TAFs complex containing the unprocessed TFIIAalphabeta precursor and the TFIIAgamma subunit."
      Mitsiou D.J., Stunnenberg H.G.
      Mol. Cell 6:527-537(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, IDENTIFICATION IN A COMPLEX WITH GTF2A1 AND TBP.

    Entry informationi

    Entry nameiT2AG_HUMAN
    AccessioniPrimary (citable) accession number: P52657
    Secondary accession number(s): A8MYQ7, Q6FGB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3