Reviewed, UniProtKB/Swiss-Prot P52657 (T2AG_HUMAN)

Names and origin

Protein names

Recommended name:
    Transcription initiation factor IIA subunit 2
Alternative name(s):
    General transcription factor IIA subunit 2
    Transcription initiation factor IIA gamma chain
      Short name=TFIIA-gamma
    TFIIA p12 subunit
      Short name=TFIIA-12
      Short name=TFIIAS

Gene names

Name:	GTF2A2
Synonyms:	TF2A2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	109 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Ref.10
Subunit structure	TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12). Interacts with NCOA6 general coactivator By similarity. TFIIA forms a complex with TBP. Interacts with SV40 Large T antigen.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the TFIIA subunit 2 family.

Ontologies

Keywords
Biological process	Host-virus interaction Transcription Transcription regulation
Cellular component	Nucleus
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	RNA elongation from RNA polymerase II promoter Inferred from Experiment. Source: Reactome RNA polymerase II transcriptional preinitiation complex assembly Ref.2 Inferred from direct assay. Source: UniProtKB general transcription from RNA polymerase II promoter Ref.1 Ref.3 Inferred from direct assay. Source: UniProtKB interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of gene-specific transcription from RNA polymerase II promoter Ref.2 Inferred from direct assay. Source: UniProtKB positive regulation of transcription factor activity Inferred from direct assay. Source: UniProtKB
Cellular component	transcription factor TFIIA complex Ref.1 Ref.2 Ref.3 Inferred from direct assay. Source: UniProtKB
Molecular function	TATA-binding protein binding Ref.3 Inferred from physical interaction. Source: UniProtKB general RNA polymerase II transcription factor activity Ref.1 Ref.3 Inferred from direct assay. Source: UniProtKB protein heterodimerization activity Inferred from physical interaction. Source: UniProtKB protein homodimerization activity Inferred from physical interaction. Source: UniProtKB transcription coactivator activity Ref.2 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-1045262,EBI-1045262
GTF2A1	P52655	1	EBI-1045262,EBI-389518
GTF2A1	P52655-1	1	EBI-1045262,EBI-1019093
TBP	P20226	2	EBI-1045262,EBI-355371
TBPL1	P62380	1	EBI-1045262,EBI-716225

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 109

109

Transcription initiation factor IIA subunit 2

PRO_0000194042

Secondary structure

1

.

.

.

.

.

.

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.

.

.

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109

Helix Strand Turn

Details...

Helix

4 – 7

4

Helix

10 – 24

15

Helix

30 – 50

21

Beta strand

55 – 68

14

Beta strand

71 – 82

12

Beta strand

87 – 98

12

Sequences

	Sequence		Length	Mass (Da)
	P52657-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 938E7DC8B9982AE8	FASTA	109	12,457
	10 20 30 40 50 60 MAYQLYRNTT LGNSLQESLD ELIQSQQITP QLALQVLLQF DKAINAALAQ RVRNRVNFRG 70 80 90 100 SLNTYRFCDN VWTFVLNDVE FREVTELIKV DKVKIVACDG KNTGSNTTE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription." Sun X., Ma D., Sheldon M., Yeung K., Reinberg D. Genes Dev. 8:2336-2348(1994) [PubMed: 7958900] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription." Ozer J., Moore P.A., Bolden A.H., Lee A., Rosen C.A., Lieberman P.M. Genes Dev. 8:2324-2335(1994) [PubMed: 7958899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription." Dejong J., Bernstein R., Roeder R.G. Proc. Natl. Acad. Sci. U.S.A. 92:3313-3317(1995) [PubMed: 7724559] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[8]	"Simian virus 40 large T antigen stabilizes the TATA-binding protein-TFIIA complex on the TATA element." Damania B., Lieberman P., Alwine J.C. Mol. Cell. Biol. 18:3926-3935(1998) [PubMed: 9632777] [Abstract] Cited for: INTERACTION WITH SV40 LARGE T ANTIGEN.
[9]	"A nuclear factor ASC-2, as a cancer-amplified transcriptional coactivator essential for ligand-dependent transactivation by nuclear receptors in vivo." Lee S.-K., Anzick S.L., Choi J.-E., Bubendorf L., Guan X.-Y., Jung Y.-K., Kallioniemi O.-P., Kononen J., Trent J.M., Azorsa D., Jhun B.-H., Cheong J.H., Lee Y.C., Meltzer P.S., Lee J.W. J. Biol. Chem. 274:34283-34293(1999) [PubMed: 10567404] [Abstract] Cited for: INTERACTION WITH NCOA6.
[10]	"TAC, a TBP-sans-TAFs complex containing the unprocessed TFIIAalphabeta precursor and the TFIIAgamma subunit." Mitsiou D.J., Stunnenberg H.G. Mol. Cell 6:527-537(2000) [PubMed: 11030333] [Abstract] Cited for: FUNCTION, SUBUNIT, IDENTIFICATION IN A COMPLEX WITH GTF2A1 AND TBP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X81713 mRNA. Translation: CAA57357.1.
U14193 mRNA. Translation: AAA64951.1.
U21242 mRNA. Translation: AAB58247.1.
BT007362 mRNA. Translation: AAP36026.1.
CR542192 mRNA. Translation: CAG46989.1.
CH471082 Genomic DNA. Translation: EAW77573.1.
BC000287 mRNA. Translation: AAH00287.1.
BC001919 mRNA. Translation: AAH01919.1.

IPI

IPI00004353.

PIR

I38952.

RefSeq

NP_004483.1.

UniGene

Hs.512934

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NVP	X-ray	2.10	D	2-109	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P52657. 5 interactions.

STRING

P52657.

Proteomic databases

PRIDE

P52657.

Genome annotation databases

Ensembl

ENST00000267869; ENSP00000267869; ENSG00000140307; Homo sapiens. [Genome view]
ENST00000396060; ENSP00000379372; ENSG00000140307; Homo sapiens. [Genome view]
ENST00000396061; ENSP00000379373; ENSG00000140307; Homo sapiens. [Genome view]
ENST00000396063; ENSP00000379375; ENSG00000140307; Homo sapiens. [Genome view]
ENST00000396064; ENSP00000379376; ENSG00000140307; Homo sapiens. [Genome view]

GeneID

2958.

KEGG

hsa:2958.

UCSC

uc002agg.1. human.

Organism-specific databases

CTD

2958.

GeneCards

GC15M057718.

H-InvDB

HIX0012296.

HGNC

HGNC:4647. GTF2A2.

MIM

600519. gene.

PharmGKB

PA29034.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P52657.

HOVERGEN

P52657.

OMA

VACDGKS.

Enzyme and pathway databases

Reactome

REACT_1788. Transcription.
REACT_6185. HIV Infection.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

P52657.

Bgee

P52657.

CleanEx

HS_GTF2A2.

Genevestigator

P52657.

GermOnline

ENSG00000140307. Homo sapiens.

Family and domain databases

InterPro

IPR009088. TFIIA_b-brl.
IPR003194. TFIIA_gsu.
IPR015871. TFIIA_gsu_C.
IPR015872. TFIIA_gsu_N.
[Graphical view]

Gene3D

G3DSA:2.30.18.10. TFIIA_betabarrel. 1 hit.
G3DSA:1.10.287.190. TFIIA_gsu_N. 1 hit.

PANTHER

PTHR10966. TFIIA_gamma. 1 hit.

Pfam

PF02751. TFIIA_gamma_C. 1 hit.
PF02268. TFIIA_gamma_N. 1 hit.
[Graphical view]

PIRSF

PIRSF009415. Hum_TFIIA_gamma. 1 hit.

ProDom

PD009224. TFIIA_gamma. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other Resources

NextBio

11726.

SOURCE

Search...

Entry information

Entry name

T2AG_HUMAN

Accession

Primary (citable) accession number: P52657
Secondary accession number(s): Q6FGB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.