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P52657

- T2AG_HUMAN

UniProt

P52657 - T2AG_HUMAN

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Protein
Transcription initiation factor IIA subunit 2
Gene
GTF2A2, TF2A2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.1 Publication

GO - Molecular functioni

  1. TBP-class protein binding Source: BHF-UCL
  2. protein binding Source: IntAct
  3. protein heterodimerization activity Source: BHF-UCL
  4. protein homodimerization activity Source: BHF-UCL
  5. transcription coactivator activity Source: BHF-UCL
  6. transcription factor binding Source: BHF-UCL
Complete GO annotation...

GO - Biological processi

  1. RNA polymerase II transcriptional preinitiation complex assembly Source: BHF-UCL
  2. gene expression Source: Reactome
  3. positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  4. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  5. transcription elongation from RNA polymerase II promoter Source: Reactome
  6. transcription from RNA polymerase II promoter Source: BHF-UCL
  7. transcription initiation from RNA polymerase II promoter Source: Reactome
  8. viral process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_6233. Transcription of the HIV genome.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6332. HIV Transcription Initiation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIA subunit 2
Alternative name(s):
General transcription factor IIA subunit 2
TFIIA p12 subunit
Short name:
TFIIA-12
Short name:
TFIIAS
Transcription initiation factor IIA gamma chain
Short name:
TFIIA-gamma
Gene namesi
Name:GTF2A2
Synonyms:TF2A2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:4647. GTF2A2.

Subcellular locationi

GO - Cellular componenti

  1. cell junction Source: HPA
  2. nucleoplasm Source: Reactome
  3. nucleus Source: HPA
  4. transcription factor TFIIA complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29034.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 109109Transcription initiation factor IIA subunit 2
PRO_0000194042Add
BLAST

Proteomic databases

MaxQBiP52657.
PaxDbiP52657.
PRIDEiP52657.

PTM databases

PhosphoSiteiP52657.

Expressioni

Gene expression databases

ArrayExpressiP52657.
BgeeiP52657.
CleanExiHS_GTF2A2.
GenevestigatoriP52657.

Organism-specific databases

HPAiHPA056239.

Interactioni

Subunit structurei

TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12). Interacts with NCOA6 general coactivator By similarity. TFIIA forms a complex with TBP. Interacts with SV40 Large T antigen.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TBPP202262EBI-1045262,EBI-355371

Protein-protein interaction databases

BioGridi109213. 9 interactions.
IntActiP52657. 2 interactions.
STRINGi9606.ENSP00000379372.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 74
Helixi10 – 2415
Helixi30 – 5021
Beta strandi55 – 6814
Beta strandi71 – 8212
Beta strandi87 – 9812

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NVPX-ray2.10D2-109[»]
ProteinModelPortaliP52657.
SMRiP52657. Positions 3-99.

Miscellaneous databases

EvolutionaryTraceiP52657.

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIA subunit 2 family.

Phylogenomic databases

eggNOGiCOG5123.
HOGENOMiHOG000167251.
HOVERGENiHBG000826.
InParanoidiP52657.
KOiK03123.
OMAiCKNGDAI.
OrthoDBiEOG7RFTKP.
PhylomeDBiP52657.
TreeFamiTF315131.

Family and domain databases

Gene3Di1.10.287.190. 1 hit.
2.30.18.10. 1 hit.
InterProiIPR009083. TFIIA_a-hlx.
IPR009088. TFIIA_b-brl.
IPR003194. TFIIA_gsu.
IPR015871. TFIIA_gsu_C.
IPR015872. TFIIA_gsu_N.
[Graphical view]
PANTHERiPTHR10966. PTHR10966. 1 hit.
PfamiPF02751. TFIIA_gamma_C. 1 hit.
PF02268. TFIIA_gamma_N. 1 hit.
[Graphical view]
PIRSFiPIRSF009415. Hum_TFIIA_gamma. 1 hit.
ProDomiPD009224. TFIIA_gsu_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.

Sequencei

Sequence statusi: Complete.

P52657-1 [UniParc]FASTAAdd to Basket

« Hide

MAYQLYRNTT LGNSLQESLD ELIQSQQITP QLALQVLLQF DKAINAALAQ    50
RVRNRVNFRG SLNTYRFCDN VWTFVLNDVE FREVTELIKV DKVKIVACDG 100
KNTGSNTTE 109
Length:109
Mass (Da):12,457
Last modified:October 1, 1996 - v1
Checksum:i938E7DC8B9982AE8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81713 mRNA. Translation: CAA57357.1.
U14193 mRNA. Translation: AAA64951.1.
U21242 mRNA. Translation: AAB58247.1.
BT007362 mRNA. Translation: AAP36026.1.
CR542192 mRNA. Translation: CAG46989.1.
AC092755 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77573.1.
BC000287 mRNA. Translation: AAH00287.1.
BC001919 mRNA. Translation: AAH01919.1.
CCDSiCCDS10173.1.
PIRiI38952.
RefSeqiNP_004483.1. NM_004492.2.
XP_005254381.1. XM_005254324.1.
XP_005254382.1. XM_005254325.1.
UniGeneiHs.512934.

Genome annotation databases

EnsembliENST00000396060; ENSP00000379372; ENSG00000140307.
ENST00000396061; ENSP00000379373; ENSG00000140307.
ENST00000396063; ENSP00000379375; ENSG00000140307.
GeneIDi2958.
KEGGihsa:2958.
UCSCiuc002agg.3. human.

Polymorphism databases

DMDMi1729806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81713 mRNA. Translation: CAA57357.1 .
U14193 mRNA. Translation: AAA64951.1 .
U21242 mRNA. Translation: AAB58247.1 .
BT007362 mRNA. Translation: AAP36026.1 .
CR542192 mRNA. Translation: CAG46989.1 .
AC092755 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77573.1 .
BC000287 mRNA. Translation: AAH00287.1 .
BC001919 mRNA. Translation: AAH01919.1 .
CCDSi CCDS10173.1.
PIRi I38952.
RefSeqi NP_004483.1. NM_004492.2.
XP_005254381.1. XM_005254324.1.
XP_005254382.1. XM_005254325.1.
UniGenei Hs.512934.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1NVP X-ray 2.10 D 2-109 [» ]
ProteinModelPortali P52657.
SMRi P52657. Positions 3-99.
ModBasei Search...

Protein-protein interaction databases

BioGridi 109213. 9 interactions.
IntActi P52657. 2 interactions.
STRINGi 9606.ENSP00000379372.

Chemistry

BindingDBi P52657.

PTM databases

PhosphoSitei P52657.

Polymorphism databases

DMDMi 1729806.

Proteomic databases

MaxQBi P52657.
PaxDbi P52657.
PRIDEi P52657.

Protocols and materials databases

DNASUi 2958.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000396060 ; ENSP00000379372 ; ENSG00000140307 .
ENST00000396061 ; ENSP00000379373 ; ENSG00000140307 .
ENST00000396063 ; ENSP00000379375 ; ENSG00000140307 .
GeneIDi 2958.
KEGGi hsa:2958.
UCSCi uc002agg.3. human.

Organism-specific databases

CTDi 2958.
GeneCardsi GC15M059930.
HGNCi HGNC:4647. GTF2A2.
HPAi HPA056239.
MIMi 600519. gene.
neXtProti NX_P52657.
PharmGKBi PA29034.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5123.
HOGENOMi HOG000167251.
HOVERGENi HBG000826.
InParanoidi P52657.
KOi K03123.
OMAi CKNGDAI.
OrthoDBi EOG7RFTKP.
PhylomeDBi P52657.
TreeFami TF315131.

Enzyme and pathway databases

Reactomei REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_6233. Transcription of the HIV genome.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6332. HIV Transcription Initiation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

EvolutionaryTracei P52657.
GenomeRNAii 2958.
NextBioi 11726.
PROi P52657.
SOURCEi Search...

Gene expression databases

ArrayExpressi P52657.
Bgeei P52657.
CleanExi HS_GTF2A2.
Genevestigatori P52657.

Family and domain databases

Gene3Di 1.10.287.190. 1 hit.
2.30.18.10. 1 hit.
InterProi IPR009083. TFIIA_a-hlx.
IPR009088. TFIIA_b-brl.
IPR003194. TFIIA_gsu.
IPR015871. TFIIA_gsu_C.
IPR015872. TFIIA_gsu_N.
[Graphical view ]
PANTHERi PTHR10966. PTHR10966. 1 hit.
Pfami PF02751. TFIIA_gamma_C. 1 hit.
PF02268. TFIIA_gamma_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF009415. Hum_TFIIA_gamma. 1 hit.
ProDomi PD009224. TFIIA_gsu_N. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription."
    Sun X., Ma D., Sheldon M., Yeung K., Reinberg D.
    Genes Dev. 8:2336-2348(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription."
    Ozer J., Moore P.A., Bolden A.H., Lee A., Rosen C.A., Lieberman P.M.
    Genes Dev. 8:2324-2335(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription."
    Dejong J., Bernstein R., Roeder R.G.
    Proc. Natl. Acad. Sci. U.S.A. 92:3313-3317(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  9. "Simian virus 40 large T antigen stabilizes the TATA-binding protein-TFIIA complex on the TATA element."
    Damania B., Lieberman P., Alwine J.C.
    Mol. Cell. Biol. 18:3926-3935(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SV40 LARGE T ANTIGEN.
  10. "A nuclear factor ASC-2, as a cancer-amplified transcriptional coactivator essential for ligand-dependent transactivation by nuclear receptors in vivo."
    Lee S.-K., Anzick S.L., Choi J.-E., Bubendorf L., Guan X.-Y., Jung Y.-K., Kallioniemi O.-P., Kononen J., Trent J.M., Azorsa D., Jhun B.-H., Cheong J.H., Lee Y.C., Meltzer P.S., Lee J.W.
    J. Biol. Chem. 274:34283-34293(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NCOA6.
  11. "TAC, a TBP-sans-TAFs complex containing the unprocessed TFIIAalphabeta precursor and the TFIIAgamma subunit."
    Mitsiou D.J., Stunnenberg H.G.
    Mol. Cell 6:527-537(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, IDENTIFICATION IN A COMPLEX WITH GTF2A1 AND TBP.

Entry informationi

Entry nameiT2AG_HUMAN
AccessioniPrimary (citable) accession number: P52657
Secondary accession number(s): A8MYQ7, Q6FGB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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