P52656 (T2AG_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor IIA subunit 2

Alternative name(s):
General transcription factor IIA subunit 2
TFIIA p14 subunit
Short name=TFIIA-14
Transcription initiation factor IIA gamma chain
Short name=TFIIA-gamma
dTFIIA-S

Gene names

Name:	TfIIA-S
ORF Names:	CG5163

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	106 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.
Subunit structure	TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p30), a beta (p20) and a gamma (p14) subunit. Ref.1
Subcellular location	Nucleus.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the TFIIA subunit 2 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro
Cellular component	transcription factor TFIIA complex Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 106	106	Transcription initiation factor IIA subunit 2		PRO_0000194048
Experimental info
	Sequence conflict	40	1	F → L in AAM29483. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P52656 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 978556B6AA8B31CA	FASTA	106	12,193
	10 20 30 40 50 60 MSYQLYRNTT LGNTLQESLD ELIQYGQITP GLAFKVLLQF DKSINNALNQ RVKARVTFKA 70 80 90 100 GKLNTYRFCD NVWTLMLNDV EFREVHEIVK VDKVKIVACD GKSGEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation." Yokomori K., Zeidler M.P., Chen J.-L., Verrijzer C.P., Mlodzik M., Tjian R. Genes Dev. 8:2313-2323(1994) [PubMed: 7958898] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 42-59, SUBUNIT. Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83271 Genomic DNA. Translation: CAA58244.1. AE014297 Genomic DNA. Translation: AAF56199.1. AY113478 mRNA. Translation: AAM29483.1. BT044069 mRNA. Translation: ACH92134.1.
PIR	A55121. A54883.
RefSeq	NP_524467.1. NM_079743.3.
UniGene	Dm.20591.
3D structure databases
ProteinModelPortal	P52656.
SMR	P52656. Positions 3-100.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-23236N.
IntAct	P52656. 1 interaction.
MINT	MINT-982942.
STRING	P52656.
Proteomic databases
PRIDE	P52656.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0084526; FBpp0083912; FBgn0013347.
GeneID	42822.
KEGG	dme:Dmel_CG5163.
NMPDR	fig|7227.3.peg.14305.
UCSC	CG5163-RA. d. melanogaster.
Organism-specific databases
CTD	42822.
FlyBase	FBgn0013347. TfIIA-S.
Phylogenomic databases
eggNOG	inNOG10084.
GeneTree	EMGT00050000016711.
InParanoid	P52656.
OMA	VACDGKS.
OrthoDB	EOG42V6ZT.
PhylomeDB	P52656.
Gene expression databases
Bgee	P52656.
GermOnline	CG5163. Drosophila melanogaster.
Family and domain databases
InterPro	IPR009083. TFIIA_a-hlx. IPR009088. TFIIA_b-brl. IPR003194. TFIIA_gsu. IPR015871. TFIIA_gsu_C. IPR015872. TFIIA_gsu_N. [Graphical view]
Gene3D	G3DSA:2.30.18.10. TFIIA_betabarrel. 1 hit. G3DSA:1.10.287.190. TFIIA_gsu_N. 1 hit.
KO	K03123.
PANTHER	PTHR10966. TFIIA_gamma. 1 hit.
Pfam	PF02751. TFIIA_gamma_C. 1 hit. PF02268. TFIIA_gamma_N. 1 hit. [Graphical view]
PIRSF	PIRSF009415. Hum_TFIIA_gamma. 1 hit.
ProDom	PD009224. TFIIA_gsu_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF50784. TFIIA_betabarrel. 1 hit. SSF47396. TFIIA_helical. 1 hit.
ProtoNet	Search...
Other
NextBio	830743.

Entry information

Entry name

T2AG_DROME

Accession

Primary (citable) accession number: P52656
Secondary accession number(s): B5RIE5, Q8MYY8, Q9VCG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project