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Protein

Cytochrome b-245 heavy chain

Gene

CYBB

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes.

Cofactori

FADCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi15Iron (heme axial ligand)Curated1
Metal bindingi29Iron (heme axial ligand)Curated1
Metal bindingi123Iron (heme axial ligand)Curated1
Metal bindingi136Iron (heme axial ligand)Curated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi252 – 258FADSequence analysis7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Oxidoreductase, Voltage-gated channel
Biological processElectron transport, Ion transport, Transport
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-245 heavy chain (EC:1.-.-.-)
Alternative name(s):
CGD91-phox
Cytochrome b(558) subunit beta
Short name:
Cytochrome b558 subunit beta
Heme-binding membrane glycoprotein gp91phox
Neutrophil cytochrome b 91 kDa polypeptide
gp91-1
gp91-phox
p22 phagocyte B-cytochrome
Gene namesi
Name:CYBB
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini‹1 – 16CytoplasmicSequence analysisAdd BLAST›16
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Topological domaini38 – 87ExtracellularSequence analysisAdd BLAST50
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Topological domaini109 – 114CytoplasmicSequence analysis6
Transmembranei115 – 135HelicalSequence analysisAdd BLAST21
Topological domaini136 – 175ExtracellularSequence analysisAdd BLAST40
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 484CytoplasmicSequence analysisAdd BLAST288

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000210147‹1 – 484Cytochrome b-245 heavy chainAdd BLAST›484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi46N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi63N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi161N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylated on Ser and Thr residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP52649.
PeptideAtlasiP52649.
PRIDEiP52649.

Interactioni

Subunit structurei

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1. Interacts with calprotectin (S100A8/9). Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000029153.

Structurei

3D structure databases

ProteinModelPortaliP52649.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 201Ferric oxidoreductaseAdd BLAST197
Domaini201 – 311FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST111

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216669.
HOVERGENiHBG003760.
InParanoidiP52649.
KOiK08008.

Family and domain databases

InterProiView protein in InterPro
IPR000778. Cyt_b245_heavy_chain.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
PfamiView protein in Pfam
PF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiView protein in PROSITE
PS51384. FAD_FR. 1 hit.

Sequencei

Sequence statusi: Fragment.

P52649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
STRVRRQLDR NLTFHKMVAW MIALHATIHT IAHLFNVEWC VNARVNNSDP
60 70 80 90 100
YSIALSDIGD KPNETYLNFV RQRIKNPEGG LYVAVTRLAG ITGVVITLCL
110 120 130 140 150
ILIITSSTKT IRRSYFEVFW YTHHLFVIFF IGLAIHGAER IVRRQTPKSL
160 170 180 190 200
LVHDPKACAQ NISQWGKIKD CPIPEFAGNP PMTWKWIVGP MFLYLCERLV
210 220 230 240 250
RFWRSQQKVV ITKVVTHPFK TIELQMKKKG FRMEVGQYIF VKRPAVSKLE
260 270 280 290 300
WHPFTLTSAP EEDFFSIHIR IVGDWTEGLF KACGCDKQEF QDAWKLPKIA
310 320 330 340 350
VDGPFGTASE DVFSYQVVML VGAGIGVTPF ASILKSVWYK YCNNATNLRL
360 370 380 390 400
KKIYFYWLCR DTHAFEWFAD LLQLLETQMQ ERNNAGFLSY NIYLTGWDES
410 420 430 440 450
QANHFAVHHD EEKDVITGLK QKTLYGRPNW DNEFKTIASQ HPTTRIGVFL
460 470 480
CGPEALAETL NKQCISNSDS SPRGVHFIFN KENF
Length:484
Mass (Da):55,830
Last modified:May 30, 2000 - v2
Checksum:i065AB9B6920CFE02
GO

Sequence cautioni

The sequence AAA64634 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02476 mRNA. Translation: AAA64634.1. Different initiation.
PIRiS52077.
RefSeqiNP_999208.1. NM_214043.2.
UniGeneiSsc.151.
Ssc.97679.

Genome annotation databases

GeneIDi397108.
KEGGissc:397108.

Similar proteinsi

Entry informationi

Entry nameiCY24B_PIG
AccessioniPrimary (citable) accession number: P52649
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: May 10, 2017
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome