UniProtKB - P52647 (NIFJ_ECOLI)
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Protein
Probable pyruvate-flavodoxin oxidoreductase
Gene
ydbK
Organism
Escherichia coli (strain K12)
Status
Functioni
Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin.Curated
Catalytic activityi
Pyruvate + CoA + oxidized flavodoxin = acetyl-CoA + CO2 + reduced flavodoxin.
Cofactori
[4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 689 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 692 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 695 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 699 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 745 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 748 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 751 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 755 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 819 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 822 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 847 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 1071 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- iron ion binding Source: InterPro
- pyruvate-flavodoxin oxidoreductase activity Source: EcoCyc
- thiamine pyrophosphate binding Source: InterPro
GO - Biological processi
- electron transport chain Source: InterPro
- response to oxidative stress Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Transport |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6701-MONOMER MetaCyc:G6701-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ydbK Ordered Locus Names:b1378, JW1372 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Organism-specific databases
EcoGenei | EG13183 ydbK |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215557 | 1 – 1174 | Probable pyruvate-flavodoxin oxidoreductaseAdd BLAST | 1174 |
Proteomic databases
EPDi | P52647 |
PaxDbi | P52647 |
PRIDEi | P52647 |
Interactioni
Protein-protein interaction databases
BioGridi | 426167623 interactors. |
DIPi | DIP-11636N |
IntActi | P52647 10 interactors. |
STRINGi | 316385.ECDH10B_1502 |
Structurei
3D structure databases
ProteinModelPortali | P52647 |
SMRi | P52647 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 680 – 709 | 4Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST | 30 | |
Domaini | 736 – 765 | 4Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST | 30 |
Sequence similaritiesi
Belongs to the NifJ family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG4105D95 Bacteria COG0674 LUCA COG1013 LUCA COG1014 LUCA |
HOGENOMi | HOG000266425 |
InParanoidi | P52647 |
KOi | K03737 |
OMAi | NTVMQVC |
PhylomeDBi | P52647 |
Family and domain databases
Gene3Di | 3.40.50.9201 hit 3.40.920.101 hit 4.10.780.101 hit |
InterProi | View protein in InterPro IPR017896 4Fe4S_Fe-S-bd IPR017900 4Fe4S_Fe_S_CS IPR033412 PFOR_II IPR037112 Pyrv-flavodox_OxR_EKR_sf IPR019456 Pyrv-flavodox_OxRtase_EKR IPR019752 Pyrv/ketoisovalerate_OxRed_cat IPR002880 Pyrv_Fd/Flavodoxin_OxRdtase_N IPR011895 Pyrv_flavodox_OxRed IPR002869 Pyrv_flavodox_OxRed_cen IPR029061 THDP-binding IPR011766 TPP_enzyme-bd_C IPR009014 Transketo_C/PFOR_II |
Pfami | View protein in Pfam PF10371 EKR, 1 hit PF17147 PFOR_II, 1 hit PF01558 POR, 1 hit PF01855 POR_N, 1 hit PF02775 TPP_enzyme_C, 1 hit |
PIRSFi | PIRSF000159 NifJ, 1 hit |
SMARTi | View protein in SMART SM00890 EKR, 1 hit |
SUPFAMi | SSF52518 SSF52518, 2 hits SSF52922 SSF52922, 1 hit SSF53323 SSF53323, 1 hit |
TIGRFAMsi | TIGR02176 pyruv_ox_red, 1 hit |
PROSITEi | View protein in PROSITE PS00198 4FE4S_FER_1, 2 hits PS51379 4FE4S_FER_2, 2 hits |
i Sequence
Sequence statusi: Complete.
P52647-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MITIDGNGAV ASVAFRTSEV IAIYPITPSS TMAEQADAWA GNGLKNVWGD
60 70 80 90 100
TPRVVEMQSE AGAIATVHGA LQTGALSTSF TSSQGLLLMI PTLYKLAGEL
110 120 130 140 150
TPFVLHVAAR TVATHALSIF GDHSDVMAVR QTGCAMLCAA NVQEAQDFAL
160 170 180 190 200
ISQIATLKSR VPFIHFFDGF RTSHEINKIV PLADDTILDL MPQVEIDAHR
210 220 230 240 250
ARALNPEHPV IRGTSANPDT YFQSREATNP WYNAVYDHVE QAMNDFSAAT
260 270 280 290 300
GRQYQPFEYY GHPQAERVII LMGSAIGTCE EVVDELLTRG EKVGVLKVRL
310 320 330 340 350
YRPFSAKHLL QALPGSVRSV AVLDRTKEPG AQAEPLYLDV MTALAEAFNN
360 370 380 390 400
GERETLPRVI GGRYGLSSKE FGPDCVLAVF AELNAAKPKA RFTVGIYDDV
410 420 430 440 450
TNLSLPLPEN TLPNSAKLEA LFYGLGSDGS VSATKNNIKI IGNSTPWYAQ
460 470 480 490 500
GYFVYDSKKA GGLTVSHLRV SEQPIRSAYL ISQADFVGCH QLQFIDKYQM
510 520 530 540 550
AERLKPGGIF LLNTPYSADE VWSRLPQEVQ AVLNQKKARF YVINAAKIAR
560 570 580 590 600
ECGLAARINT VMQMAFFHLT QILPGDSALA ELQGAIAKSY SSKGQDLVER
610 620 630 640 650
NWQALALARE SVEEVPLQPV NPHSANRPPV VSDAAPDFVK TVTAAMLAGL
660 670 680 690 700
GDALPVSALP PDGTWPMGTT RWEKRNIAEE IPIWKEELCT QCNHCVAACP
710 720 730 740 750
HSAIRAKVVP PEAMENAPAS LHSLDVKSRD MRGQKYVLQV APEDCTGCNL
760 770 780 790 800
CVEVCPAKDR QNPEIKAINM MSRLEHVEEE KINYDFFLNL PEIDRSKLER
810 820 830 840 850
IDIRTSQLIT PLFEYSGACS GCGETPYIKL LTQLYGDRML IANATGCSSI
860 870 880 890 900
YGGNLPSTPY TTDANGRGPA WANSLFEDNA EFGLGFRLTV DQHRVRVLRL
910 920 930 940 950
LDQFADKIPA ELLTALKSDA TPEVRREQVA ALRQQLNDVA EAHELLRDAD
960 970 980 990 1000
ALVEKSIWLI GGDGWAYDIG FGGLDHVLSL TENVNILVLD TQCYSNTGGQ
1010 1020 1030 1040 1050
ASKATPLGAV TKFGEHGKRK ARKDLGVSMM MYGHVYVAQI SLGAQLNQTV
1060 1070 1080 1090 1100
KAIQEAEAYP GPSLIIAYSP CEEHGYDLAL SHDQMRQLTA TGFWPLYRFD
1110 1120 1130 1140 1150
PRRADEGKLP LALDSRPPSE APEETLLHEQ RFRRLNSQQP EVAEQLWKDA
1160 1170
AADLQKRYDF LAQMAGKAEK SNTD
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 40 | A → R (PubMed:9025293).Curated | 1 | |
Sequence conflicti | 51 | T → S (PubMed:9025293).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74460.1 AP009048 Genomic DNA Translation: BAA14982.1 U36928 Genomic DNA No translation available. |
PIRi | E64888 |
RefSeqi | NP_415896.1, NC_000913.3 WP_000628244.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74460; AAC74460; b1378 BAA14982; BAA14982; BAA14982 |
GeneIDi | 946587 |
KEGGi | ecj:JW1372 eco:b1378 |
PATRICi | fig|511145.12.peg.1440 |
Similar proteinsi
Entry informationi
Entry namei | NIFJ_ECOLI | |
Accessioni | P52647Primary (citable) accession number: P52647 Secondary accession number(s): P77238 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 1, 1997 | |
Last modified: | March 28, 2018 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |