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Protein

D-lactate dehydrogenase

Gene

ldhA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Fermentative lactate dehydrogenase.

Catalytic activityi

(R)-lactate + NAD+ = pyruvate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei174 – 1741NADBy similarity
Binding sitei211 – 2111NADBy similarity
Active sitei234 – 2341By similarity
Binding sitei258 – 2581NADBy similarity
Active sitei263 – 2631By similarity
Active sitei295 – 2951Proton donorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi154 – 1552NADBy similarity
Nucleotide bindingi205 – 2062NADBy similarity
Nucleotide bindingi232 – 2343NADBy similarity

GO - Molecular functioni

  • D-lactate dehydrogenase activity Source: EcoCyc
  • NADH binding Source: EcoliWiki

GO - Biological processi

  • mixed acid fermentation Source: EcoCyc
  • response to heat Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:DLACTDEHYDROGNAD-MONOMER.
ECOL316407:JW1375-MONOMER.
MetaCyc:DLACTDEHYDROGNAD-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
D-lactate dehydrogenase (EC:1.1.1.28)
Short name:
D-LDH
Alternative name(s):
Fermentative lactate dehydrogenase
Gene namesi
Name:ldhA
Synonyms:hslI, htpH
Ordered Locus Names:b1380, JW1375
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13186. ldhA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329D-lactate dehydrogenasePRO_0000075952Add
BLAST

Proteomic databases

EPDiP52643.
PaxDbiP52643.
PRIDEiP52643.

Interactioni

Protein-protein interaction databases

BioGridi4259502. 6 interactions.
DIPiDIP-10087N.
IntActiP52643. 3 interactions.
STRINGi511145.b1380.

Structurei

3D structure databases

ProteinModelPortaliP52643.
SMRiP52643. Positions 1-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C5I. Bacteria.
COG1052. LUCA.
HOGENOMiHOG000136695.
InParanoidiP52643.
KOiK03778.
OMAiVIVTAHQ.
OrthoDBiEOG61VZ8G.
PhylomeDBiP52643.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52643-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLAVYSTKQ YDKKYLQQVN ESFGFELEFF DFLLTEKTAK TANGCEAVCI
60 70 80 90 100
FVNDDGSRPV LEELKKHGVK YIALRCAGFN NVDLDAAKEL GLKVVRVPAY
110 120 130 140 150
DPEAVAEHAI GMMMTLNRRI HRAYQRTRDA NFSLEGLTGF TMYGKTAGVI
160 170 180 190 200
GTGKIGVAML RILKGFGMRL LAFDPYPSAA ALELGVEYVD LPTLFSESDV
210 220 230 240 250
ISLHCPLTPE NYHLLNEAAF EQMKNGVMIV NTSRGALIDS QAAIEALKNQ
260 270 280 290 300
KIGSLGMDVY ENERDLFFED KSNDVIQDDV FRRLSACHNV LFTGHQAFLT
310 320
AEALTSISQT TLQNLSNLEK GETCPNELV
Length:329
Mass (Da):36,535
Last modified:October 1, 1996 - v1
Checksum:iEF85419988438D6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36928 Genomic DNA. Translation: AAB51772.1.
U00096 Genomic DNA. Translation: AAC74462.1.
AP009048 Genomic DNA. Translation: BAA14990.1.
PIRiG64888.
RefSeqiNP_415898.1. NC_000913.3.
WP_000762236.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74462; AAC74462; b1380.
BAA14990; BAA14990; BAA14990.
GeneIDi946315.
KEGGiecj:JW1375.
eco:b1380.
PATRICi32118046. VBIEscCol129921_1443.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36928 Genomic DNA. Translation: AAB51772.1.
U00096 Genomic DNA. Translation: AAC74462.1.
AP009048 Genomic DNA. Translation: BAA14990.1.
PIRiG64888.
RefSeqiNP_415898.1. NC_000913.3.
WP_000762236.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP52643.
SMRiP52643. Positions 1-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259502. 6 interactions.
DIPiDIP-10087N.
IntActiP52643. 3 interactions.
STRINGi511145.b1380.

Proteomic databases

EPDiP52643.
PaxDbiP52643.
PRIDEiP52643.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74462; AAC74462; b1380.
BAA14990; BAA14990; BAA14990.
GeneIDi946315.
KEGGiecj:JW1375.
eco:b1380.
PATRICi32118046. VBIEscCol129921_1443.

Organism-specific databases

EchoBASEiEB2978.
EcoGeneiEG13186. ldhA.

Phylogenomic databases

eggNOGiENOG4105C5I. Bacteria.
COG1052. LUCA.
HOGENOMiHOG000136695.
InParanoidiP52643.
KOiK03778.
OMAiVIVTAHQ.
OrthoDBiEOG61VZ8G.
PhylomeDBiP52643.

Enzyme and pathway databases

BioCyciEcoCyc:DLACTDEHYDROGNAD-MONOMER.
ECOL316407:JW1375-MONOMER.
MetaCyc:DLACTDEHYDROGNAD-MONOMER.

Miscellaneous databases

PROiP52643.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli."
    Bunch P.K., Mat-Jan F., Lee N., Clark D.P.
    Microbiology 143:187-195(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Characterization of twenty-six new heat shock genes of Escherichia coli."
    Chuang S.E., Blattner F.R.
    J. Bacteriol. 175:5242-5252(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION AS AN HEAT-SHOCK GENE.
  6. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiLDHD_ECOLI
AccessioniPrimary (citable) accession number: P52643
Secondary accession number(s): P78152
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 16, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.