Reviewed,
UniProtKB/Swiss-Prot P52643 (LDHD_ECOLI)
Last modified
February 9, 2010.
Version 80.
History...
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90%,
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: D-lactate dehydrogenase Short name=D-LDH EC=1.1.1.28 Alternative name(s): Fermentative lactate dehydrogenase | ||||||
| Gene names |
| ||||||
| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 329 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Fermentative lactate dehydrogenase. |
| Catalytic activity | (R)-lactate + NAD+ = pyruvate + NADH. |
| Sequence similarities | Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Stress response |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to heat Ref.5Inferred from expression pattern. Source: UniProtKB |
| Molecular function | D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 329 | 329 | D-lactate dehydrogenase | PRO_0000075952 | |||||
Regions | |||||||||
| Nucleotide binding | 154 – 155 | 2 | NAD By similarity | ||||||
| Nucleotide binding | 232 – 234 | 3 | NAD By similarity | ||||||
| Nucleotide binding | 295 – 298 | 4 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 234 | 1 | By similarity | ||||||
| Active site | 263 | 1 | By similarity | ||||||
| Active site | 295 | 1 | Proton donor By similarity | ||||||
| Binding site | 258 | 1 | NAD By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli." Bunch P.K., Mat-Jan F., Lee N., Clark D.P. Microbiology 143:187-195(1997) [PubMed: 9025293] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T.DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [3] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Characterization of twenty-six new heat shock genes of Escherichia coli." Chuang S.E., Blattner F.R. J. Bacteriol. 175:5242-5252(1993) [PubMed: 8349564] [Abstract] Cited for: CHARACTERIZATION AS AN HEAT-SHOCK GENE. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U36928 Genomic DNA. Translation: AAB51772.1. U00096 Genomic DNA. Translation: AAC74462.1. AP009048 Genomic DNA. Translation: BAA14990.1. |
| PIR | G64888. |
| RefSeq | AP_002007.1. NP_415898.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P52643. |
2-D gel databases | |
| ECO2DBASE | D033.4. 6TH EDITION. |
Genome annotation databases | |
| GeneID | 946315. |
| GenomeReviews | Gene locus JW1375 in contig AP009048_GR. Gene locus b1380 in contig U00096_GR. |
| KEGG | ecj:JW1375. eco:b1380. |
Organism-specific databases | |
| EchoBASE | EB2978. |
| EcoGene | EG13186. ldhA. |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG1052. |
| HOGENOM | HBG731446. |
| OMA | YEEEAQL. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:DLACTDEHYDROGNAD-MONOMER. ECOL168927:B1380-MONOMER. MetaCyc:DLACTDEHYDROGNAD-MONOMER. |
Gene expression databases | |
| Genevestigator | P52643. |
Family and domain databases | |
| InterPro | IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| Pfam | PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view] |
| PROSITE | PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LDHD_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P52643 Secondary accession number(s): P78152 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

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