Reviewed, UniProtKB/Swiss-Prot P52643 (LDHD_ECOLI)

Names and origin

Protein names

Recommended name:
    D-lactate dehydrogenase
      Short name=D-LDH
    EC=1.1.1.28
Alternative name(s):
    Fermentative lactate dehydrogenase

Gene names

Name:	ldhA
Synonyms:	hslI, htpH
Ordered Locus Names:	b1380, JW1375

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Fermentative lactate dehydrogenase.
Catalytic activity	(R)-lactate + NAD+ = pyruvate + NADH.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Biological process	Stress response
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to heat Ref.5 Inferred from expression pattern. Source: UniProtKB
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	D-lactate dehydrogenase		PRO_0000075952
Regions
	Nucleotide binding	154 – 155	2	NAD By similarity
	Nucleotide binding	232 – 234	3	NAD By similarity
	Nucleotide binding	295 – 298	4	NAD By similarity
Sites
	Active site	234	1	By similarity
	Active site	263	1	By similarity
	Active site	295	1	Proton donor By similarity
	Binding site	258	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52643-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: EF85419988438D6D	FASTA	329	36,535
	10 20 30 40 50 60 MKLAVYSTKQ YDKKYLQQVN ESFGFELEFF DFLLTEKTAK TANGCEAVCI FVNDDGSRPV 70 80 90 100 110 120 LEELKKHGVK YIALRCAGFN NVDLDAAKEL GLKVVRVPAY DPEAVAEHAI GMMMTLNRRI 130 140 150 160 170 180 HRAYQRTRDA NFSLEGLTGF TMYGKTAGVI GTGKIGVAML RILKGFGMRL LAFDPYPSAA 190 200 210 220 230 240 ALELGVEYVD LPTLFSESDV ISLHCPLTPE NYHLLNEAAF EQMKNGVMIV NTSRGALIDS 250 260 270 280 290 300 QAAIEALKNQ KIGSLGMDVY ENERDLFFED KSNDVIQDDV FRRLSACHNV LFTGHQAFLT 310 320 AEALTSISQT TLQNLSNLEK GETCPNELV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli." Bunch P.K., Mat-Jan F., Lee N., Clark D.P. Microbiology 143:187-195(1997) [PubMed: 9025293] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Characterization of twenty-six new heat shock genes of Escherichia coli." Chuang S.E., Blattner F.R. J. Bacteriol. 175:5242-5252(1993) [PubMed: 8349564] [Abstract] Cited for: CHARACTERIZATION AS AN HEAT-SHOCK GENE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U36928 Genomic DNA. Translation: AAB51772.1. U00096 Genomic DNA. Translation: AAC74462.1. AP009048 Genomic DNA. Translation: BAA14990.1.
PIR	G64888.
RefSeq	AP_002007.1. NP_415898.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P52643.
2-D gel databases
ECO2DBASE	D033.4. 6TH EDITION.
Genome annotation databases
GeneID	946315.
GenomeReviews	Gene locus JW1375 in contig AP009048_GR. Gene locus b1380 in contig U00096_GR.
KEGG	ecj:JW1375. eco:b1380.
Organism-specific databases
EchoBASE	EB2978.
EcoGene	EG13186. ldhA.
CMR	Search...
Phylogenomic databases
eggNOG	COG1052.
HOGENOM	HBG731446.
OMA	YEEEAQL.
Enzyme and pathway databases
BioCyc	EcoCyc:DLACTDEHYDROGNAD-MONOMER. ECOL168927:B1380-MONOMER. MetaCyc:DLACTDEHYDROGNAD-MONOMER.
Gene expression databases
Genevestigator	P52643.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_ECOLI

Accession

Primary (citable) accession number: P52643
Secondary accession number(s): P78152

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)