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P52643 (LDHD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-lactate dehydrogenase
Short name=D-LDH
EC=1.1.1.28
Alternative name(s):
Fermentative lactate dehydrogenase
Gene names
Name:ldhA
Synonyms:hslI, htpH
Ordered Locus Names:b1380, JW1375
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Fermentative lactate dehydrogenase.

Catalytic activity

(R)-lactate + NAD+ = pyruvate + NADH.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
   Biological processStress response
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processresponse to heat

Inferred from expression pattern Ref.5. Source: EcoliWiki

   Molecular functionD-lactate dehydrogenase activity

Inferred from electronic annotation. Source: EC

NAD binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329D-lactate dehydrogenase
PRO_0000075952

Regions

Nucleotide binding154 – 1552NAD By similarity
Nucleotide binding232 – 2343NAD By similarity
Nucleotide binding295 – 2984NAD By similarity

Sites

Active site2341 By similarity
Active site2631 By similarity
Active site2951Proton donor By similarity
Binding site2581NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
P52643 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: EF85419988438D6D

FASTA32936,535
        10         20         30         40         50         60 
MKLAVYSTKQ YDKKYLQQVN ESFGFELEFF DFLLTEKTAK TANGCEAVCI FVNDDGSRPV 

        70         80         90        100        110        120 
LEELKKHGVK YIALRCAGFN NVDLDAAKEL GLKVVRVPAY DPEAVAEHAI GMMMTLNRRI 

       130        140        150        160        170        180 
HRAYQRTRDA NFSLEGLTGF TMYGKTAGVI GTGKIGVAML RILKGFGMRL LAFDPYPSAA 

       190        200        210        220        230        240 
ALELGVEYVD LPTLFSESDV ISLHCPLTPE NYHLLNEAAF EQMKNGVMIV NTSRGALIDS 

       250        260        270        280        290        300 
QAAIEALKNQ KIGSLGMDVY ENERDLFFED KSNDVIQDDV FRRLSACHNV LFTGHQAFLT 

       310        320 
AEALTSISQT TLQNLSNLEK GETCPNELV

« Hide

References

« Hide 'large scale' references
[1]"The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli."
Bunch P.K., Mat-Jan F., Lee N., Clark D.P.
Microbiology 143:187-195(1997) [PubMed: 9025293] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Characterization of twenty-six new heat shock genes of Escherichia coli."
Chuang S.E., Blattner F.R.
J. Bacteriol. 175:5242-5252(1993) [PubMed: 8349564] [Abstract]
Cited for: CHARACTERIZATION AS AN HEAT-SHOCK GENE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U36928 Genomic DNA. Translation: AAB51772.1.
U00096 Genomic DNA. Translation: AAC74462.1.
AP009048 Genomic DNA. Translation: BAA14990.1.
PIRG64888.
RefSeqNP_415898.1. NC_000913.2.

3D structure databases

ProteinModelPortalP52643.
SMRP52643. Positions 1-329.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10087N.
IntActP52643. 3 interactions.

2D gel databases

ECO2DBASED033.4. 6TH EDITION.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000002021; EBESCP00000002021; EBESCG00000001646.
EBESCT00000014636; EBESCP00000013927; EBESCG00000013697.
GeneID946315.
GenomeReviewsGene locus JW1375 in contig AP009048_GR.
Gene locus b1380 in contig U00096_GR.
KEGGecj:JW1375.
eco:b1380.
PATRIC32118046. VBIEscCol129921_1443.

Organism-specific databases

EchoBASEEB2978.
EcoGeneEG13186. ldhA.

Phylogenomic databases

eggNOGCOG1052.
GeneTreeEBGT00050000009079.
HOGENOMHBG731446.
OMAMAGFGCQ.
PhylomeDBP52643.
ProtClustDBCLSK869554.

Enzyme and pathway databases

BioCycEcoCyc:DLACTDEHYDROGNAD-MONOMER.
MetaCyc:DLACTDEHYDROGNAD-MONOMER.

Gene expression databases

GenevestigatorP52643.

Family and domain databases

InterProIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KOK03778.
PfamPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLDHD_ECOLI
AccessionPrimary (citable) accession number: P52643
Secondary accession number(s): P78152
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 25, 2012
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

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