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P52639

- L_BDVV

UniProt

P52639 - L_BDVV

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Protein

RNA-directed RNA polymerase L

Gene
L
Organism
Borna disease virus (strain V) (BDV)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently three subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. In replicase mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 3 domains:
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
mRNA guanylyltransferase (EC:2.7.7.-)
Gene namesi
Name:L
OrganismiBorna disease virus (strain V) (BDV)
Taxonomic identifieri928296 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesBornaviridaeBornavirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Bradypodidae (three-fingered sloths) [TaxID: 9352]
Capra hircus (Goat) [TaxID: 9925]
Cervidae (deer) [TaxID: 9850]
Crocidura leucodon (Bicoloured white-toothed shrew) (Celebes shrew) [TaxID: 109474]
Equidae (horses) [TaxID: 9788]
Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
Hexaprotodon liberiensis (Pygmy hippopotamus) (Choeropsis liberiensis) [TaxID: 56798]
Lama glama (Llama) [TaxID: 9844]
Oryctolagus cuniculus (Rabbit) [TaxID: 9986]
Ovis aries (Sheep) [TaxID: 9940]
Struthio camelus (Ostrich) [TaxID: 8801]
Varecia variegata (Black-and-white ruffed lemur) (Lemur variegatus) [TaxID: 9455]
Vicugna pacos (Alpaca) (Lama pacos) [TaxID: 30538]
ProteomesiUP000007804: Genome

Subcellular locationi

Virion Reviewed prediction. Host nucleus 1 Publication

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
  2. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17111711RNA-directed RNA polymerase LPRO_0000079207Add
BLAST

Interactioni

Subunit structurei

Interacts with the P protein.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini543 – 715173RdRp catalyticAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi844 – 8529Nuclear localization signal

Sequence similaritiesi

Family and domain databases

InterProiIPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view]
PfamiPF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view]
PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform Large structural protein (identifier: P52639-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSFHASLLRE EETPRPVAGI NRTDQSLKNP LLGTEVSFCL KSSSLPHHVR     50
ALGQIKARNL ASCDYYLLFR QVVLPPEVYP IGVLIRAAEA ILTVIVSAWK 100
LDHMTKTLYS SVRYALTNPR VRAQLELHIA YQRIVGQVSY SREADIGPKR 150
LGNMSLQFIQ SLVIATIDTT SCLMTYNHFL AAADTAKSRC HLLIASVVQG 200
ALWEQGSFLD HIINMIDIID SINLPHDDYF TIIKSIFPYS QGLVMGRHNV 250
SVSSDFASVF AIPELCPQLD SLLKKLLQLD PVLLLMVSSV QKSWYFPEIR 300
MVDGSREQLH KMRVELETPQ ALLSYGHTLL SIFRAEFIKG YVSKNAKWPP 350
VHLLPGCDKS IKNARELGRW SPAFDRRWQL FEKVVILRIA DLDMDPDFND 400
IVSDKAIISS RRDWVFEYNA AAFWKKYGER LERPPARSGP SRLVNALIDG 450
RLDNIPALLE PFYRGAVEFE DRLTVLVPKE KELKVKGRFF SKQTLAIRIY 500
QVVAEAALKN EVMPYLKTHS MTMSSTALTH LLNRLSHTIT KGDSFVINLD 550
YSSWCNGFRP ELQAPICRQL DQMFNCGYFF RTGCTLPCFT TFIIQDRFNP 600
PYSLSGEPVE DGVTCAVGTK TMGEGMRQKL WTILTSCWEI IALREINVTF 650
NILGQGDNQT IIIHKSASQN NQLLAERALG ALYKHARLAG HNLKVEECWV 700
SDCLYEYGKK LFFRGVPVPG CLKQLSRVTD STGELFPNLY SKLACLTSSC 750
LSAAMADTSP WVALATGVCL YLIELYVELP PAIIQDESLL TTLCLVGPSI 800
GGLPTPATLP SVFFRGMSDP LPFQLALLQT LIKTTGVTCS LVNRVVKLRI 850
APYPDWLSLV TDPTSLNIAQ VYRPERQIRR WIEEAIATSS HSSRIATFFQ 900
QPLTEMAQLL ARDLSTMMPL RPRDMSALFA LSNVAYGLSI IDLFQKSSTV 950
VSASQAVHIE DVALESVRYK ESIIQGLLDT TEGYNMQPYL EGCTYLAAKQ 1000
LRRLTWGRDL VGVTMPFVAE QFHPHSSVGA KAELYLDAII YCPQETLRSH 1050
HLTTRGDQPL YLGSNTAVKV QRGEITGLAK SRAANLVKDT LVLHQWYKVR 1100
KVTDPHLNTL MARFLLEKGY TSDARPSIQG GTLTHRLPSR GDSRQGLTGY 1150
VNILSTWLRF SSDYLHSFSK SSDDYTIHFQ HVFTYGCLYA DSVIRSGGVI 1200
STPYLLSASC KTCFEKIDSE EFVLACEPQY RGAEWLISKP VTVPEQITDA 1250
EVEFDPCVSA SYCLGILIGK SFLVDIRASG HDIMEQRTWA NLERFSVSDM 1300
QKLPWSIVIR SLWRFLIGAR LLQFEKAGLI RMLYAATGPT FSFLMKVFQD 1350
SALLMDCAPL DRLSPRINFH SRGDLVAKLV LLPFINPGIV EIEVSGINSK 1400
YHAVSEANMD LYIAAAKSVG VKPTQFVEET NDFTARGHHH GCYSLSWSKS 1450
RNQSQVLKMV VRKLKLCVLY IYPTVDPAVA LDLCHLPALT IILVLGGDPA 1500
YYERLLEMDL CGAVSSRVDI PHSLAARTHR GFAVGPDAGP GVIRLDRLES 1550
VCYAHPCLEE LEFNAYLDSE LVDISDMCCL PLATPCKALF RPIYRSLQSF 1600
RLALMDNYSF VMDLIMIRGL DIRPHLEEFD ELLVVGQHIL GQPVLVEVVY 1650
YVGVVRKRPV LARHPWSADL KRITVGGRAP CPSAARLRDE DCQGSLLVGL 1700
PAGLTQLLII D 1711
Length:1,711
Mass (Da):191,734
Last modified:December 20, 2005 - v2
Checksum:i554DF018E0E17877
GO
Isoform Matrix protein (identifier: P0C795-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P0C795.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:142
Mass (Da):16,244
GO
Isoform Envelope glycoprotein p57 precursor (identifier: P52638-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P52638.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:503
Mass (Da):56,652
GO

Sequence cautioni

The sequence AAA20228.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1526 – 15261A → G.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04608 Genomic RNA. Translation: AAA20228.1. Different initiation.
AJ311521 Genomic RNA. Translation: CAC70639.1.
RefSeqiNP_042024.2. NC_001607.1.

Genome annotation databases

GeneIDi1494405.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04608 Genomic RNA. Translation: AAA20228.1 . Different initiation.
AJ311521 Genomic RNA. Translation: CAC70639.1 .
RefSeqi NP_042024.2. NC_001607.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1494405.

Family and domain databases

InterProi IPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view ]
Pfami PF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view ]
PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Conservation of coding potential and terminal sequences in four different isolates of Borna disease virus."
    Pleschka S., Staeheli P., Kolodziejek J., Richt J.A., Nowotny N., Schwemmle M.
    J. Gen. Virol. 82:2681-2690(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: V/FR.
  3. "Expression and characterization of the Borna disease virus polymerase."
    Walker M.P., Jordan I., Briese T., Fischer N., Lipkin W.I.
    J. Virol. 74:4425-4428(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH P PROTEIN.
  4. "Characterization of the nuclear localization signal of the borna disease virus polymerase."
    Walker M.P., Lipkin W.I.
    J. Virol. 76:8460-8467(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: REVIEW.

Entry informationi

Entry nameiL_BDVV
AccessioniPrimary (citable) accession number: P52639
Secondary accession number(s): Q912Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 20, 2005
Last modified: April 16, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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