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P52639

- L_BDVV

UniProt

P52639 - L_BDVV

Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Borna disease virus (strain V) (BDV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently three subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. In replicase mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase L
    Short name:
    Protein L
    Alternative name(s):
    Large structural protein
    Replicase
    Transcriptase
    Including the following 3 domains:
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    mRNA guanylyltransferase (EC:2.7.7.-)
    Gene namesi
    Name:L
    OrganismiBorna disease virus (strain V) (BDV)
    Taxonomic identifieri928296 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesBornaviridaeBornavirus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    Bradypodidae (three-fingered sloths) [TaxID: 9352]
    Capra hircus (Goat) [TaxID: 9925]
    Cervidae (deer) [TaxID: 9850]
    Crocidura leucodon (Bicoloured white-toothed shrew) (Celebes shrew) [TaxID: 109474]
    Equidae (horses) [TaxID: 9788]
    Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
    Hexaprotodon liberiensis (Pygmy hippopotamus) (Choeropsis liberiensis) [TaxID: 56798]
    Lama glama (Llama) [TaxID: 9844]
    Oryctolagus cuniculus (Rabbit) [TaxID: 9986]
    Ovis aries (Sheep) [TaxID: 9940]
    Struthio camelus (Ostrich) [TaxID: 8801]
    Varecia variegata (Black-and-white ruffed lemur) (Lemur variegatus) [TaxID: 9455]
    Vicugna pacos (Alpaca) (Lama pacos) [TaxID: 30538]
    ProteomesiUP000007804: Genome

    Subcellular locationi

    Virion Curated. Host nucleus 1 Publication

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 17111711RNA-directed RNA polymerase LPRO_0000079207Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with the P protein.1 Publication

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini543 – 715173RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi844 – 8529Nuclear localization signal

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR026890. Mononeg_mRNAcap.
    IPR014023. Mononeg_RNA_pol_cat.
    IPR025786. RNA-direct_RNA_pol_L.
    [Graphical view]
    PfamiPF14318. Mononeg_mRNAcap. 1 hit.
    PF00946. Mononeg_RNA_pol. 1 hit.
    [Graphical view]
    PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform Large structural protein (identifier: P52639-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSFHASLLRE EETPRPVAGI NRTDQSLKNP LLGTEVSFCL KSSSLPHHVR     50
    ALGQIKARNL ASCDYYLLFR QVVLPPEVYP IGVLIRAAEA ILTVIVSAWK 100
    LDHMTKTLYS SVRYALTNPR VRAQLELHIA YQRIVGQVSY SREADIGPKR 150
    LGNMSLQFIQ SLVIATIDTT SCLMTYNHFL AAADTAKSRC HLLIASVVQG 200
    ALWEQGSFLD HIINMIDIID SINLPHDDYF TIIKSIFPYS QGLVMGRHNV 250
    SVSSDFASVF AIPELCPQLD SLLKKLLQLD PVLLLMVSSV QKSWYFPEIR 300
    MVDGSREQLH KMRVELETPQ ALLSYGHTLL SIFRAEFIKG YVSKNAKWPP 350
    VHLLPGCDKS IKNARELGRW SPAFDRRWQL FEKVVILRIA DLDMDPDFND 400
    IVSDKAIISS RRDWVFEYNA AAFWKKYGER LERPPARSGP SRLVNALIDG 450
    RLDNIPALLE PFYRGAVEFE DRLTVLVPKE KELKVKGRFF SKQTLAIRIY 500
    QVVAEAALKN EVMPYLKTHS MTMSSTALTH LLNRLSHTIT KGDSFVINLD 550
    YSSWCNGFRP ELQAPICRQL DQMFNCGYFF RTGCTLPCFT TFIIQDRFNP 600
    PYSLSGEPVE DGVTCAVGTK TMGEGMRQKL WTILTSCWEI IALREINVTF 650
    NILGQGDNQT IIIHKSASQN NQLLAERALG ALYKHARLAG HNLKVEECWV 700
    SDCLYEYGKK LFFRGVPVPG CLKQLSRVTD STGELFPNLY SKLACLTSSC 750
    LSAAMADTSP WVALATGVCL YLIELYVELP PAIIQDESLL TTLCLVGPSI 800
    GGLPTPATLP SVFFRGMSDP LPFQLALLQT LIKTTGVTCS LVNRVVKLRI 850
    APYPDWLSLV TDPTSLNIAQ VYRPERQIRR WIEEAIATSS HSSRIATFFQ 900
    QPLTEMAQLL ARDLSTMMPL RPRDMSALFA LSNVAYGLSI IDLFQKSSTV 950
    VSASQAVHIE DVALESVRYK ESIIQGLLDT TEGYNMQPYL EGCTYLAAKQ 1000
    LRRLTWGRDL VGVTMPFVAE QFHPHSSVGA KAELYLDAII YCPQETLRSH 1050
    HLTTRGDQPL YLGSNTAVKV QRGEITGLAK SRAANLVKDT LVLHQWYKVR 1100
    KVTDPHLNTL MARFLLEKGY TSDARPSIQG GTLTHRLPSR GDSRQGLTGY 1150
    VNILSTWLRF SSDYLHSFSK SSDDYTIHFQ HVFTYGCLYA DSVIRSGGVI 1200
    STPYLLSASC KTCFEKIDSE EFVLACEPQY RGAEWLISKP VTVPEQITDA 1250
    EVEFDPCVSA SYCLGILIGK SFLVDIRASG HDIMEQRTWA NLERFSVSDM 1300
    QKLPWSIVIR SLWRFLIGAR LLQFEKAGLI RMLYAATGPT FSFLMKVFQD 1350
    SALLMDCAPL DRLSPRINFH SRGDLVAKLV LLPFINPGIV EIEVSGINSK 1400
    YHAVSEANMD LYIAAAKSVG VKPTQFVEET NDFTARGHHH GCYSLSWSKS 1450
    RNQSQVLKMV VRKLKLCVLY IYPTVDPAVA LDLCHLPALT IILVLGGDPA 1500
    YYERLLEMDL CGAVSSRVDI PHSLAARTHR GFAVGPDAGP GVIRLDRLES 1550
    VCYAHPCLEE LEFNAYLDSE LVDISDMCCL PLATPCKALF RPIYRSLQSF 1600
    RLALMDNYSF VMDLIMIRGL DIRPHLEEFD ELLVVGQHIL GQPVLVEVVY 1650
    YVGVVRKRPV LARHPWSADL KRITVGGRAP CPSAARLRDE DCQGSLLVGL 1700
    PAGLTQLLII D 1711
    Length:1,711
    Mass (Da):191,734
    Last modified:December 20, 2005 - v2
    Checksum:i554DF018E0E17877
    GO
    Isoform Matrix protein (identifier: P0C795-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P0C795.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:142
    Mass (Da):16,244
    GO
    Isoform Envelope glycoprotein p57 precursor (identifier: P52638-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P52638.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:503
    Mass (Da):56,652
    GO

    Sequence cautioni

    The sequence AAA20228.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti1526 – 15261A → G.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04608 Genomic RNA. Translation: AAA20228.1. Different initiation.
    AJ311521 Genomic RNA. Translation: CAC70639.1.
    RefSeqiNP_042024.2. NC_001607.1.

    Genome annotation databases

    GeneIDi1494405.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04608 Genomic RNA. Translation: AAA20228.1 . Different initiation.
    AJ311521 Genomic RNA. Translation: CAC70639.1 .
    RefSeqi NP_042024.2. NC_001607.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1494405.

    Family and domain databases

    InterProi IPR026890. Mononeg_mRNAcap.
    IPR014023. Mononeg_RNA_pol_cat.
    IPR025786. RNA-direct_RNA_pol_L.
    [Graphical view ]
    Pfami PF14318. Mononeg_mRNAcap. 1 hit.
    PF00946. Mononeg_RNA_pol. 1 hit.
    [Graphical view ]
    PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Conservation of coding potential and terminal sequences in four different isolates of Borna disease virus."
      Pleschka S., Staeheli P., Kolodziejek J., Richt J.A., Nowotny N., Schwemmle M.
      J. Gen. Virol. 82:2681-2690(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: V/FR.
    3. "Expression and characterization of the Borna disease virus polymerase."
      Walker M.P., Jordan I., Briese T., Fischer N., Lipkin W.I.
      J. Virol. 74:4425-4428(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH P PROTEIN.
    4. "Characterization of the nuclear localization signal of the borna disease virus polymerase."
      Walker M.P., Lipkin W.I.
      J. Virol. 76:8460-8467(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. Cited for: REVIEW.

    Entry informationi

    Entry nameiL_BDVV
    AccessioniPrimary (citable) accession number: P52639
    Secondary accession number(s): Q912Z6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3