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P52639

- L_BDVV

UniProt

P52639 - L_BDVV

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Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Borna disease virus (strain V) (BDV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently three subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. In replicase mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 3 domains:
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
mRNA guanylyltransferase (EC:2.7.7.-)
Gene namesi
Name:L
OrganismiBorna disease virus (strain V) (BDV)
Taxonomic identifieri928296 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesBornaviridaeBornavirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Bradypodidae (three-fingered sloths) [TaxID: 9352]
Capra hircus (Goat) [TaxID: 9925]
Cervidae (deer) [TaxID: 9850]
Crocidura leucodon (Bicoloured white-toothed shrew) (Celebes shrew) [TaxID: 109474]
Equidae (horses) [TaxID: 9788]
Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
Hexaprotodon liberiensis (Pygmy hippopotamus) (Choeropsis liberiensis) [TaxID: 56798]
Lama glama (Llama) [TaxID: 9844]
Oryctolagus cuniculus (Rabbit) [TaxID: 9986]
Ovis aries (Sheep) [TaxID: 9940]
Struthio camelus (Ostrich) [TaxID: 8801]
Varecia variegata (Black-and-white ruffed lemur) (Lemur variegatus) [TaxID: 9455]
Vicugna pacos (Alpaca) (Lama pacos) [TaxID: 30538]
ProteomesiUP000007804: Genome

Subcellular locationi

Virion Curated. Host nucleus 1 Publication

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
  2. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17111711RNA-directed RNA polymerase LPRO_0000079207Add
BLAST

Interactioni

Subunit structurei

Interacts with the P protein.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini543 – 715173RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi844 – 8529Nuclear localization signal

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view]
PfamiPF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view]
PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform Large structural protein (identifier: P52639-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFHASLLRE EETPRPVAGI NRTDQSLKNP LLGTEVSFCL KSSSLPHHVR
60 70 80 90 100
ALGQIKARNL ASCDYYLLFR QVVLPPEVYP IGVLIRAAEA ILTVIVSAWK
110 120 130 140 150
LDHMTKTLYS SVRYALTNPR VRAQLELHIA YQRIVGQVSY SREADIGPKR
160 170 180 190 200
LGNMSLQFIQ SLVIATIDTT SCLMTYNHFL AAADTAKSRC HLLIASVVQG
210 220 230 240 250
ALWEQGSFLD HIINMIDIID SINLPHDDYF TIIKSIFPYS QGLVMGRHNV
260 270 280 290 300
SVSSDFASVF AIPELCPQLD SLLKKLLQLD PVLLLMVSSV QKSWYFPEIR
310 320 330 340 350
MVDGSREQLH KMRVELETPQ ALLSYGHTLL SIFRAEFIKG YVSKNAKWPP
360 370 380 390 400
VHLLPGCDKS IKNARELGRW SPAFDRRWQL FEKVVILRIA DLDMDPDFND
410 420 430 440 450
IVSDKAIISS RRDWVFEYNA AAFWKKYGER LERPPARSGP SRLVNALIDG
460 470 480 490 500
RLDNIPALLE PFYRGAVEFE DRLTVLVPKE KELKVKGRFF SKQTLAIRIY
510 520 530 540 550
QVVAEAALKN EVMPYLKTHS MTMSSTALTH LLNRLSHTIT KGDSFVINLD
560 570 580 590 600
YSSWCNGFRP ELQAPICRQL DQMFNCGYFF RTGCTLPCFT TFIIQDRFNP
610 620 630 640 650
PYSLSGEPVE DGVTCAVGTK TMGEGMRQKL WTILTSCWEI IALREINVTF
660 670 680 690 700
NILGQGDNQT IIIHKSASQN NQLLAERALG ALYKHARLAG HNLKVEECWV
710 720 730 740 750
SDCLYEYGKK LFFRGVPVPG CLKQLSRVTD STGELFPNLY SKLACLTSSC
760 770 780 790 800
LSAAMADTSP WVALATGVCL YLIELYVELP PAIIQDESLL TTLCLVGPSI
810 820 830 840 850
GGLPTPATLP SVFFRGMSDP LPFQLALLQT LIKTTGVTCS LVNRVVKLRI
860 870 880 890 900
APYPDWLSLV TDPTSLNIAQ VYRPERQIRR WIEEAIATSS HSSRIATFFQ
910 920 930 940 950
QPLTEMAQLL ARDLSTMMPL RPRDMSALFA LSNVAYGLSI IDLFQKSSTV
960 970 980 990 1000
VSASQAVHIE DVALESVRYK ESIIQGLLDT TEGYNMQPYL EGCTYLAAKQ
1010 1020 1030 1040 1050
LRRLTWGRDL VGVTMPFVAE QFHPHSSVGA KAELYLDAII YCPQETLRSH
1060 1070 1080 1090 1100
HLTTRGDQPL YLGSNTAVKV QRGEITGLAK SRAANLVKDT LVLHQWYKVR
1110 1120 1130 1140 1150
KVTDPHLNTL MARFLLEKGY TSDARPSIQG GTLTHRLPSR GDSRQGLTGY
1160 1170 1180 1190 1200
VNILSTWLRF SSDYLHSFSK SSDDYTIHFQ HVFTYGCLYA DSVIRSGGVI
1210 1220 1230 1240 1250
STPYLLSASC KTCFEKIDSE EFVLACEPQY RGAEWLISKP VTVPEQITDA
1260 1270 1280 1290 1300
EVEFDPCVSA SYCLGILIGK SFLVDIRASG HDIMEQRTWA NLERFSVSDM
1310 1320 1330 1340 1350
QKLPWSIVIR SLWRFLIGAR LLQFEKAGLI RMLYAATGPT FSFLMKVFQD
1360 1370 1380 1390 1400
SALLMDCAPL DRLSPRINFH SRGDLVAKLV LLPFINPGIV EIEVSGINSK
1410 1420 1430 1440 1450
YHAVSEANMD LYIAAAKSVG VKPTQFVEET NDFTARGHHH GCYSLSWSKS
1460 1470 1480 1490 1500
RNQSQVLKMV VRKLKLCVLY IYPTVDPAVA LDLCHLPALT IILVLGGDPA
1510 1520 1530 1540 1550
YYERLLEMDL CGAVSSRVDI PHSLAARTHR GFAVGPDAGP GVIRLDRLES
1560 1570 1580 1590 1600
VCYAHPCLEE LEFNAYLDSE LVDISDMCCL PLATPCKALF RPIYRSLQSF
1610 1620 1630 1640 1650
RLALMDNYSF VMDLIMIRGL DIRPHLEEFD ELLVVGQHIL GQPVLVEVVY
1660 1670 1680 1690 1700
YVGVVRKRPV LARHPWSADL KRITVGGRAP CPSAARLRDE DCQGSLLVGL
1710
PAGLTQLLII D
Length:1,711
Mass (Da):191,734
Last modified:December 20, 2005 - v2
Checksum:i554DF018E0E17877
GO
Isoform Matrix protein (identifier: P0C795-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P0C795.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:142
Mass (Da):16,244
GO
Isoform Envelope glycoprotein p57 precursor (identifier: P52638-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P52638.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:503
Mass (Da):56,652
GO

Sequence cautioni

The sequence AAA20228.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1526 – 15261A → G.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04608 Genomic RNA. Translation: AAA20228.1. Different initiation.
AJ311521 Genomic RNA. Translation: CAC70639.1.
RefSeqiNP_042024.2. NC_001607.1.

Genome annotation databases

GeneIDi1494405.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04608 Genomic RNA. Translation: AAA20228.1 . Different initiation.
AJ311521 Genomic RNA. Translation: CAC70639.1 .
RefSeqi NP_042024.2. NC_001607.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1494405.

Family and domain databases

InterProi IPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view ]
Pfami PF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view ]
PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Conservation of coding potential and terminal sequences in four different isolates of Borna disease virus."
    Pleschka S., Staeheli P., Kolodziejek J., Richt J.A., Nowotny N., Schwemmle M.
    J. Gen. Virol. 82:2681-2690(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: V/FR.
  3. "Expression and characterization of the Borna disease virus polymerase."
    Walker M.P., Jordan I., Briese T., Fischer N., Lipkin W.I.
    J. Virol. 74:4425-4428(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH P PROTEIN.
  4. "Characterization of the nuclear localization signal of the borna disease virus polymerase."
    Walker M.P., Lipkin W.I.
    J. Virol. 76:8460-8467(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: REVIEW.

Entry informationi

Entry nameiL_BDVV
AccessioniPrimary (citable) accession number: P52639
Secondary accession number(s): Q912Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3