Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Signal transducer and activator of transcription 5B

Gene

Stat5b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.By similarity

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • DNA binding Source: RGD
  • double-stranded DNA binding Source: RGD
  • protein dimerization activity Source: UniProtKB
  • sequence-specific DNA binding Source: RGD
  • signal transducer activity Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: RGD

GO - Biological processi

  • acute-phase response Source: RGD
  • cellular response to epidermal growth factor stimulus Source: UniProtKB
  • cellular response to growth factor stimulus Source: UniProtKB
  • JAK-STAT cascade Source: RGD
  • liver development Source: RGD
  • luteinization Source: RGD
  • positive regulation of cellular component movement Source: RGD
  • positive regulation of erythrocyte differentiation Source: UniProtKB
  • positive regulation of smooth muscle cell proliferation Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • prolactin signaling pathway Source: UniProtKB
  • regulation of transcription, DNA-templated Source: RGD
  • response to ethanol Source: RGD
  • response to hypoxia Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to peptide hormone Source: RGD
  • transcription from RNA polymerase II promoter Source: RGD

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-1170546. Prolactin receptor signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducer and activator of transcription 5B
Gene namesi
Name:Stat5b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3774. Stat5b.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Translocated into the nucleus in response to phosphorylation.By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: RGD
  • nucleus Source: RGD

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001824321 – 786Signal transducer and activator of transcription 5BAdd BLAST786

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei90PhosphotyrosineBy similarity1
Modified residuei128PhosphoserineCombined sources1
Modified residuei699PhosphotyrosineCombined sources1

Post-translational modificationi

Tyrosine phosphorylated. Tyrosine phosphorylated in response to signaling via activated FLT3 and KIT, resulting in translocation to the nucleus. Alternatively, can be phosphorylated by JAK2 (By similarity). Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity (By similarity). Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP52632.
PRIDEiP52632.

PTM databases

iPTMnetiP52632.
PhosphoSitePlusiP52632.

Interactioni

Subunit structurei

Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1. Interacts with NMI. Interacts with SOCS7. Interacts (via SH2 domain) with INSR. Interacts with CPEB3; this inhibits STAT5B-mediated transcriptional activation.By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-60986N.
STRINGi10116.ENSRNOP00000026354.

Structurei

3D structure databases

ProteinModelPortaliP52632.
SMRiP52632.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini589 – 686SH2PROSITE-ProRule annotationAdd BLAST98

Sequence similaritiesi

Belongs to the transcription factor STAT family.Curated

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG3667. Eukaryota.
ENOG410XPN8. LUCA.
HOGENOMiHOG000230988.
HOVERGENiHBG107486.
InParanoidiP52632.
KOiK11224.
PhylomeDBiP52632.
TreeFamiTF318648.

Family and domain databases

Gene3Di1.10.532.10. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.
InterProiView protein in InterPro
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR001217. STAT.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
PANTHERiPTHR11801. PTHR11801. 1 hit.
PfamiView protein in Pfam
PF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
SMARTiView protein in SMART
SM00252. SH2. 1 hit.
SM00964. STAT_int. 1 hit.
SUPFAMiSSF47655. SSF47655. 1 hit.
SSF48092. SSF48092. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiView protein in PROSITE
PS50001. SH2. 1 hit.

Sequencei

Sequence statusi: Complete.

P52632-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMWIQAQQL QGDALHQMQA LYGQHFPIEV RHYLSQWIES QAWDSIDLDN
60 70 80 90 100
PQENIKATQL LEGLVQELQK KAEHQVGEDG FLLKIKLGHY ATQLQNTYDR
110 120 130 140 150
CPMELVRCIR HILYNEQRLV REANNGSSPA GSLADAMSQK HLQINQTFEE
160 170 180 190 200
LRLITQDTES ELKKLQQTQE YFIIQYQESL RIQAQFAQLA QLNPQERMSR
210 220 230 240 250
ETALQQKQVS LETWLQREAQ TLQQYRVELA EKHQKTLQLL RKQQTIILDD
260 270 280 290 300
ELIQWKRRQQ LAGNGGPPEG SLDVLQSWCE KLAEIIWQNR QQIRRLEHLC
310 320 330 340 350
QQLPIPGPVE EMLAEVNATI TDIISALSTS TFIIEKQPPQ VLKTQTKFAA
360 370 380 390 400
TVRLLVGGKL NVHMNPPQVK ATIISEQQAK SLLKNENTRN DYSGEILNNC
410 420 430 440 450
CVMEYHQATG TLSAHFRNMS LKRIKRSDRR GAESVTEEKF TILFDSQFSV
460 470 480 490 500
GGNELVFQVK TLSLPVVVIV HGSQDNNATA TVLWDNAFRE PGRVPFAVPD
510 520 530 540 550
KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNS SSNHLEDYNS
560 570 580 590 600
MSVSWSQFNR ENLPGRNYTF WQWFDGVMEV LKKHLKPHWN DGAILGFVNK
610 620 630 640 650
QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSQERMF WNLMPFTTRD
660 670 680 690 700
FSIRSLADRL GDLNYLIYVF PDRPKDEVYS KYYTPVPCER ATAKAADGYV
710 720 730 740 750
KPQIKQVVPE FVNASTDAGS GATYMDQAPS PVVCPQAHYN MYPQNPDSVL
760 770 780
DTDGDFDLED TMDVARRVEE LLGRPMDSQW IPHAQS
Length:786
Mass (Da):90,223
Last modified:October 1, 1996 - v1
Checksum:i52250DE6ACC8DBE3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti296L → A in CAA66141 (Ref. 2) Curated1
Sequence conflicti328S → V in CAA66141 (Ref. 2) Curated1
Sequence conflicti489R → A in CAA66141 (Ref. 2) Curated1
Sequence conflicti690R → P in CAA66141 (Ref. 2) Curated1
Sequence conflicti716T → A in CAA66141 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91988 mRNA. Translation: CAA63043.1. Different termination.
X91988 mRNA. Translation: CAA63042.1.
X97541 mRNA. Translation: CAA66141.1.
RefSeqiNP_071775.1. NM_022380.1.
UniGeneiRn.54486.

Genome annotation databases

GeneIDi25126.
KEGGirno:25126.
UCSCiRGD:3774. rat.

Similar proteinsi

Entry informationi

Entry nameiSTA5B_RAT
AccessioniPrimary (citable) accession number: P52632
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: August 30, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families