P52632 (STA5B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal transducer and activator of transcription 5B

Gene names

Name:

Stat5b

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	786 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription By similarity.
Subunit structure	Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7 By similarity. Interacts (via SH2 domain) with INSR.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Translocated into the nucleus in response to phosphorylation By similarity.
Post-translational modification	Tyrosine phosphorylated. Tyrosine phosphorylated in response to signaling via activated FLT3 and KIT, resulting in translocation to the nucleus. Alternatively, can be phosphorylated by JAK2 By similarity. Phosphoryation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity By similarity.
Sequence similarities	Belongs to the transcription factor STAT family. Contains 1 SH2 domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	SH2 domain
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	2-oxoglutarate metabolic process Inferred from electronic annotation. Source: Compara JAK-STAT cascade Inferred from direct assay PubMed 11064147. Source: RGD JAK-STAT cascade involved in growth hormone signaling pathway Inferred from electronic annotation. Source: Compara Peyer's patch development Inferred from electronic annotation. Source: Compara T cell differentiation in thymus Inferred from electronic annotation. Source: Compara T cell homeostasis Inferred from electronic annotation. Source: Compara acute-phase response Inferred from direct assay PubMed 17565389. Source: RGD allantoin metabolic process Inferred from electronic annotation. Source: Compara citrate metabolic process Inferred from electronic annotation. Source: Compara creatine metabolic process Inferred from electronic annotation. Source: Compara creatinine metabolic process Inferred from electronic annotation. Source: Compara cytokine-mediated signaling pathway Inferred from electronic annotation. Source: Compara development of secondary female sexual characteristics Inferred from electronic annotation. Source: Compara development of secondary male sexual characteristics Inferred from electronic annotation. Source: Compara fatty acid metabolic process Inferred from electronic annotation. Source: Compara female pregnancy Inferred from electronic annotation. Source: Compara isoleucine metabolic process Inferred from electronic annotation. Source: Compara lactation Inferred from electronic annotation. Source: Compara lipid storage Inferred from electronic annotation. Source: Compara liver development Inferred from direct assay PubMed 17565389. Source: RGD luteinization Inferred from direct assay PubMed 10598581. Source: RGD natural killer cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of erythrocyte differentiation Inferred from electronic annotation. Source: Compara oxaloacetate metabolic process Inferred from electronic annotation. Source: Compara positive regulation of B cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of activated T cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of cellular component movement Inferred from mutant phenotype PubMed 16527988. Source: RGD positive regulation of gamma-delta T cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of inflammatory response Inferred from electronic annotation. Source: Compara positive regulation of interleukin-2 biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of mitotic cell cycle Inferred from electronic annotation. Source: Compara positive regulation of multicellular organism growth Inferred from electronic annotation. Source: Compara positive regulation of natural killer cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of natural killer cell mediated cytotoxicity Inferred from electronic annotation. Source: Compara positive regulation of natural killer cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle cell proliferation Inferred from mutant phenotype PubMed 16527988. Source: RGD positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara progesterone metabolic process Inferred from electronic annotation. Source: Compara regulation of cell adhesion Inferred from electronic annotation. Source: Compara regulation of epithelial cell differentiation Inferred from electronic annotation. Source: Compara regulation of steroid metabolic process Inferred from electronic annotation. Source: Compara response to estradiol stimulus Inferred from electronic annotation. Source: Compara response to ethanol Inferred from expression pattern PubMed 12933671. Source: RGD response to hypoxia Inferred from expression pattern PubMed 16730240. Source: RGD response to interleukin-15 Inferred from electronic annotation. Source: Compara response to interleukin-2 Inferred from electronic annotation. Source: Compara response to interleukin-4 Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from direct assay PubMed 17327369. Source: RGD response to peptide hormone stimulus Inferred from direct assay PubMed 9092792. Source: RGD succinate metabolic process Inferred from electronic annotation. Source: Compara taurine metabolic process Inferred from electronic annotation. Source: Compara transcription from RNA polymerase II promoter Inferred from direct assay PubMed 11064147. Source: RGD valine metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from direct assay PubMed 12933671. Source: RGD nucleus Inferred from direct assay PubMed 17327369. Source: RGD
Molecular_function	RNA polymerase II core promoter sequence-specific DNA binding Inferred from electronic annotation. Source: Compara calcium ion binding Inferred from electronic annotation. Source: InterPro double-stranded DNA binding Inferred from direct assay PubMed 11064147. Source: RGD sequence-specific DNA binding Inferred from direct assay PubMed 11064147. Source: RGD sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 11064147PubMed 11562369. Source: RGD signal transducer activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 786	786	Signal transducer and activator of transcription 5B		PRO_0000182432
Regions
	Domain	589 – 686	98	SH2
Amino acid modifications
	Modified residue	128	1	Phosphoserine By similarity
	Modified residue	699	1	Phosphotyrosine; by HCK, JAK and PTK6 By similarity
Experimental info
	Sequence conflict	296	1	L → A in CAA66141. Ref.2
	Sequence conflict	328	1	S → V in CAA66141. Ref.2
	Sequence conflict	489	1	R → A in CAA66141. Ref.2
	Sequence conflict	690	1	R → P in CAA66141. Ref.2
	Sequence conflict	716	1	T → A in CAA66141. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P52632 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 52250DE6ACC8DBE3	FASTA	786	90,223
	10 20 30 40 50 60 MAMWIQAQQL QGDALHQMQA LYGQHFPIEV RHYLSQWIES QAWDSIDLDN PQENIKATQL 70 80 90 100 110 120 LEGLVQELQK KAEHQVGEDG FLLKIKLGHY ATQLQNTYDR CPMELVRCIR HILYNEQRLV 130 140 150 160 170 180 REANNGSSPA GSLADAMSQK HLQINQTFEE LRLITQDTES ELKKLQQTQE YFIIQYQESL 190 200 210 220 230 240 RIQAQFAQLA QLNPQERMSR ETALQQKQVS LETWLQREAQ TLQQYRVELA EKHQKTLQLL 250 260 270 280 290 300 RKQQTIILDD ELIQWKRRQQ LAGNGGPPEG SLDVLQSWCE KLAEIIWQNR QQIRRLEHLC 310 320 330 340 350 360 QQLPIPGPVE EMLAEVNATI TDIISALSTS TFIIEKQPPQ VLKTQTKFAA TVRLLVGGKL 370 380 390 400 410 420 NVHMNPPQVK ATIISEQQAK SLLKNENTRN DYSGEILNNC CVMEYHQATG TLSAHFRNMS 430 440 450 460 470 480 LKRIKRSDRR GAESVTEEKF TILFDSQFSV GGNELVFQVK TLSLPVVVIV HGSQDNNATA 490 500 510 520 530 540 TVLWDNAFRE PGRVPFAVPD KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNS 550 560 570 580 590 600 SSNHLEDYNS MSVSWSQFNR ENLPGRNYTF WQWFDGVMEV LKKHLKPHWN DGAILGFVNK 610 620 630 640 650 660 QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSQERMF WNLMPFTTRD FSIRSLADRL 670 680 690 700 710 720 GDLNYLIYVF PDRPKDEVYS KYYTPVPCER ATAKAADGYV KPQIKQVVPE FVNASTDAGS 730 740 750 760 770 780 GATYMDQAPS PVVCPQAHYN MYPQNPDSVL DTDGDFDLED TMDVARRVEE LLGRPMDSQW IPHAQS

References

[1]	"Transcription factors Stat3 and Stat5b are present in rat liver nuclei late in an acute phase response and bind interleukin-6 response elements." Ripperger J.A., Fritz S., Richter K., Hocke G.M., Lottspeich F., Fey G.H. J. Biol. Chem. 270:29998-30006(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	Luo G., Yu-Lee L. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Noble. Tissue: Lymph node.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X91988 mRNA. Translation: CAA63043.1. Different termination. X91988 mRNA. Translation: CAA63042.1. X97541 mRNA. Translation: CAA66141.1.
IPI	IPI00326838.
RefSeq	NP_071775.1. NM_022380.1.
UniGene	Rn.54486.
3D structure databases
ProteinModelPortal	P52632.
SMR	P52632. Positions 138-686.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000026354.
PTM databases
PhosphoSite	P52632.
Proteomic databases
PaxDb	P52632.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026354; ENSRNOP00000026354; ENSRNOG00000019075.
GeneID	25126.
KEGG	rno:25126.
UCSC	RGD:3774. rat.
Organism-specific databases
CTD	6777.
RGD	3774. Stat5b.
Phylogenomic databases
eggNOG	NOG245085.
GeneTree	ENSGT00550000074337.
HOGENOM	HOG000230988.
HOVERGEN	HBG107486.
InParanoid	P52632.
KO	K11224.
OrthoDB	EOG457560.
Enzyme and pathway databases
Reactome	REACT_109781. Immune System. REACT_111984. Signal Transduction.
Gene expression databases
Genevestigator	P52632.
GermOnline	ENSRNOG00000019075. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit. 1.10.532.10. 1 hit. 1.20.1050.20. 1 hit. 2.60.40.630. 1 hit. 3.30.505.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR008967. p53-like_TF_DNA-bd. IPR000980. SH2. IPR013800. STAT_TF_alpha. IPR015988. STAT_TF_coiled-coil. IPR001217. STAT_TF_core. IPR013801. STAT_TF_DNA-bd. IPR012345. STAT_TF_DNA-bd_sub. IPR013799. STAT_TF_prot_interaction. [Graphical view]
PANTHER	PTHR11801. PTHR11801. 1 hit.
Pfam	PF00017. SH2. 1 hit. PF01017. STAT_alpha. 1 hit. PF02864. STAT_bind. 1 hit. PF02865. STAT_int. 1 hit. [Graphical view]
SMART	SM00252. SH2. 1 hit. SM00964. STAT_int. 1 hit. [Graphical view]
SUPFAM	SSF49417. P53_like_DNA_bnd. 1 hit. SSF47655. STAT. 1 hit. SSF48092. STAT. 1 hit.
PROSITE	PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	605525.

Entry information

Entry name

STA5B_RAT

Accession

Primary (citable) accession number: P52632

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program