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Protein

Uridine phosphorylase 1

Gene

Upp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.

Catalytic activityi

Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate.

Pathway:iUMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes uracil from uridine (phosphorylase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Uridine phosphorylase (Upp2), Uridine phosphorylase (Upp1), Uridine phosphorylase (Upp2), Uridine phosphorylase (Upp2), Uridine phosphorylase (Upp2), Uridine phosphorylase 2 (Upp2), Uridine phosphorylase 1 (Upp1), Uridine phosphorylase (Upp2)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes uracil from uridine (phosphorylase route), the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei218 – 2181SubstrateBy similarity
Binding sitei220 – 2201SubstrateBy similarity

GO - Molecular functioni

  • uridine phosphorylase activity Source: MGI

GO - Biological processi

  • cellular response to glucose starvation Source: Ensembl
  • nucleotide catabolic process Source: InterPro
  • pyrimidine nucleotide metabolic process Source: MGI
  • UMP salvage Source: UniProtKB-UniPathway
  • uridine catabolic process Source: CACAO
  • uridine metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_295314. Pyrimidine salvage reactions.
REACT_317918. Pyrimidine catabolism.
UniPathwayiUPA00574; UER00633.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridine phosphorylase 1 (EC:2.4.2.3)
Short name:
UPase 1
Short name:
UrdPase 1
Gene namesi
Name:Upp1
Synonyms:Up, Upp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1097668. Upp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Uridine phosphorylase 1PRO_0000063192Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiP52624.
PaxDbiP52624.
PRIDEiP52624.

PTM databases

PhosphoSiteiP52624.

Expressioni

Inductioni

By a mixture of inflammatory cytokines: TNF-alpha, IL-1 and interferon gamma.

Gene expression databases

BgeeiP52624.
CleanExiMM_UPP1.
ExpressionAtlasiP52624. baseline and differential.
GenevisibleiP52624. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP52624. 1 interaction.
MINTiMINT-4139554.
STRINGi10090.ENSMUSP00000020677.

Structurei

3D structure databases

ProteinModelPortaliP52624.
SMRiP52624. Positions 16-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PNP/UDP phosphorylase family.Curated

Phylogenomic databases

eggNOGiCOG2820.
GeneTreeiENSGT00390000004400.
HOGENOMiHOG000231747.
HOVERGENiHBG047725.
InParanoidiP52624.
KOiK00757.
OMAiDATFKPQ.
OrthoDBiEOG79W961.
PhylomeDBiP52624.
TreeFamiTF314310.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
InterProiIPR018017. Nucleoside_phosphorylase.
IPR018016. Nucleoside_phosphorylase_CS.
IPR000845. Nucleoside_phosphorylase_d.
IPR010059. Uridine_phosphorylase_euk.
[Graphical view]
PANTHERiPTHR21234. PTHR21234. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01719. euk_UDPppase. 1 hit.
PROSITEiPS01232. PNP_UDP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52624-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATGTEAKD LENHHNDCFI QLSNPNIAAM KEDVLYHFNL STSTHDFPAM
60 70 80 90 100
FGDVKFVCVG GSSSRMNTFI KYVAAELGLD HPGKEYPNIC AGTDRYAMYK
110 120 130 140 150
AGPVLSVSHG MGIPSIGIML HELIKMLYHA RCSNITIIRI GTSGGIGLEP
160 170 180 190 200
GSVVITQQAV NECFKPEFEQ IVLGKRVIRN TNLDAQLVQE LVQCSSDLNE
210 220 230 240 250
FPMVVGNTMC TLDFYEGQGR LDGALCSYTE KDKQSYLRAA HAAGVRNIEM
260 270 280 290 300
ESSVFATMCS ACGLKAAVVC VTLLDRLQGD QINTPHDVLV EYQQRPQRLV
310
GHFIKKSLGR A
Length:311
Mass (Da):34,086
Last modified:May 23, 2003 - v2
Checksum:iA7DFC46BF9129302
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti260 – 2601S → G in BAA07916 (PubMed:7744869).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D44464 mRNA. Translation: BAA07916.1.
BC036559 mRNA. Translation: AAH36559.1.
CCDSiCCDS24432.1.
PIRiA57501.
RefSeqiNP_001152873.1. NM_001159401.1.
NP_001152874.1. NM_001159402.1.
NP_033503.2. NM_009477.3.
XP_006514730.1. XM_006514667.2.
UniGeneiMm.4610.

Genome annotation databases

EnsembliENSMUST00000020677; ENSMUSP00000020677; ENSMUSG00000020407.
ENSMUST00000101525; ENSMUSP00000099063; ENSMUSG00000020407.
ENSMUST00000164791; ENSMUSP00000127473; ENSMUSG00000020407.
GeneIDi22271.
KEGGimmu:22271.
UCSCiuc007hzw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D44464 mRNA. Translation: BAA07916.1.
BC036559 mRNA. Translation: AAH36559.1.
CCDSiCCDS24432.1.
PIRiA57501.
RefSeqiNP_001152873.1. NM_001159401.1.
NP_001152874.1. NM_001159402.1.
NP_033503.2. NM_009477.3.
XP_006514730.1. XM_006514667.2.
UniGeneiMm.4610.

3D structure databases

ProteinModelPortaliP52624.
SMRiP52624. Positions 16-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP52624. 1 interaction.
MINTiMINT-4139554.
STRINGi10090.ENSMUSP00000020677.

Chemistry

BindingDBiP52624.
ChEMBLiCHEMBL3718.

PTM databases

PhosphoSiteiP52624.

Proteomic databases

MaxQBiP52624.
PaxDbiP52624.
PRIDEiP52624.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020677; ENSMUSP00000020677; ENSMUSG00000020407.
ENSMUST00000101525; ENSMUSP00000099063; ENSMUSG00000020407.
ENSMUST00000164791; ENSMUSP00000127473; ENSMUSG00000020407.
GeneIDi22271.
KEGGimmu:22271.
UCSCiuc007hzw.2. mouse.

Organism-specific databases

CTDi7378.
MGIiMGI:1097668. Upp1.

Phylogenomic databases

eggNOGiCOG2820.
GeneTreeiENSGT00390000004400.
HOGENOMiHOG000231747.
HOVERGENiHBG047725.
InParanoidiP52624.
KOiK00757.
OMAiDATFKPQ.
OrthoDBiEOG79W961.
PhylomeDBiP52624.
TreeFamiTF314310.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00633.
ReactomeiREACT_295314. Pyrimidine salvage reactions.
REACT_317918. Pyrimidine catabolism.

Miscellaneous databases

NextBioi302379.
PROiP52624.
SOURCEiSearch...

Gene expression databases

BgeeiP52624.
CleanExiMM_UPP1.
ExpressionAtlasiP52624. baseline and differential.
GenevisibleiP52624. MM.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
InterProiIPR018017. Nucleoside_phosphorylase.
IPR018016. Nucleoside_phosphorylase_CS.
IPR000845. Nucleoside_phosphorylase_d.
IPR010059. Uridine_phosphorylase_euk.
[Graphical view]
PANTHERiPTHR21234. PTHR21234. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01719. euk_UDPppase. 1 hit.
PROSITEiPS01232. PNP_UDP_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Purification, cloning, and expression of murine uridine phosphorylase."
    Watanabe S., Hino A., Wada K., Eliason J.F., Uchida T.
    J. Biol. Chem. 270:12191-12196(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 67-86 AND 277-293.
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiUPP1_MOUSE
AccessioniPrimary (citable) accession number: P52624
Secondary accession number(s): Q8JZX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 23, 2003
Last modified: July 22, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.