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Protein

Flagellum-specific ATP synthase

Gene

fliI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable catalytic subunit of a protein translocase for flagellum-specific export, or a proton translocase involved in local circuits at the flagellum. May be involved in a specialized protein export pathway that proceeds without signal peptide cleavage.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).PROSITE-ProRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi182 – 189ATPBy similarity8

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processATP synthesis, Bacterial flagellum biogenesis, Bacterial flagellum protein export, Hydrogen ion transport, Ion transport, Protein transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G377-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellum-specific ATP synthase (EC:3.6.3.14)
Gene namesi
Name:fliI
Synonyms:fla AIII, flaC
Ordered Locus Names:b1941, JW1925
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG20266. fliI.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001446941 – 457Flagellum-specific ATP synthaseAdd BLAST457

Proteomic databases

PaxDbiP52612.
PRIDEiP52612.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-1118363,EBI-1118363

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4260388. 6 interactors.
DIPiDIP-9655N.
IntActiP52612. 23 interactors.
STRINGi316385.ECDH10B_2083.

Structurei

3D structure databases

ProteinModelPortaliP52612.
SMRiP52612.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Phylogenomic databases

eggNOGiENOG4108JIR. Bacteria.
COG1157. LUCA.
HOGENOMiHOG000257876.
InParanoidiP52612.
KOiK02412.
PhylomeDBiP52612.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR020003. ATPase_a/bsu_AS.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR032463. ATPase_T3SS_FliI.
IPR005714. ATPase_T3SS_FliI/YscN.
IPR020005. FliI_clade1.
IPR027417. P-loop_NTPase.
PANTHERiPTHR15184:SF56. PTHR15184:SF56. 1 hit.
PfamiView protein in Pfam
PF00006. ATP-synt_ab. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03496. FliI_clade1. 1 hit.
TIGR01026. fliI_yscN. 1 hit.
PROSITEiView protein in PROSITE
PS00152. ATPASE_ALPHA_BETA. 1 hit.

Sequencei

Sequence statusi: Complete.

P52612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTRLTRWLT TLDNFEAKMA QLPAVRRYGR LTRATGLVLE ATGLQLPLGA
60 70 80 90 100
TCVIERQNGS ETHEVESEVV GFNGQRLFLM PLEEVEGVLP GARVYAKNIS
110 120 130 140 150
AEGLQSGKQL PLGPALLGRV LDGSGKPLDG LPSPDTTETG ALITPPFNPL
160 170 180 190 200
QRTPIEHVLD TGVRPINALL TVGRGQRMGL FAGSGVGKSV LLGMMARYTR
210 220 230 240 250
ADVIVVGLIG ERGREVKDFI ENILGAEGRA RSVVIAAPAD VSPLLRMQGA
260 270 280 290 300
AYATRIAEDF RDRGQHVLLI MDSLTRYAMA QREIALAIGE PPATKGYPPS
310 320 330 340 350
VFAKLPALVE RAGNGISGGG SITAFYTVLT EGDDQQDPIA DSARAILDGH
360 370 380 390 400
IVLSRRLAEA GHYPAIDIEA SISRAMTALI SEQHYARVRT FKQLLSSFQR
410 420 430 440 450
NRDLVSVGAY AKGSDPMLDK AIALWPQLEG YLQQGIFERA DWEASLQGLE

RIFPTVS
Length:457
Mass (Da):49,316
Last modified:November 1, 1997 - v2
Checksum:i962D4268E8428C93
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112 – 113LG → SV in AAA82637 (Ref. 1) Curated2
Sequence conflicti185G → A in AAA82637 (Ref. 1) Curated1
Sequence conflicti239A → R in AAA82637 (Ref. 1) Curated1
Sequence conflicti248 – 250QGA → RMP in AAA82637 (Ref. 1) Curated3
Sequence conflicti278A → G in AAA82637 (Ref. 1) Curated1
Sequence conflicti399 – 400QR → HG in AAA82637 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L49147 Genomic DNA. Translation: AAA82637.1.
U00096 Genomic DNA. Translation: AAC75008.1.
AP009048 Genomic DNA. Translation: BAA15766.1.
PIRiB64958.
RefSeqiNP_416451.1. NC_000913.3.
WP_000213294.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75008; AAC75008; b1941.
BAA15766; BAA15766; BAA15766.
GeneIDi946457.
KEGGiecj:JW1925.
eco:b1941.
PATRICifig|1411691.4.peg.310.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiFLII_ECOLI
AccessioniPrimary (citable) accession number: P52612
Secondary accession number(s): P78073
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: July 5, 2017
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families