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Protein

Probable multidrug resistance protein EmrK

Gene

emrK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Part of the tripartite efflux system EmrYK-TolC, which confers resistance to various drugs.By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoCyc
  • response to antibiotic Source: EcoCyc

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:G7233-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable multidrug resistance protein EmrK
Gene namesi
Name:emrK
Ordered Locus Names:b2368, JW2365
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13282 emrK

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Topological domaini38 – 387PeriplasmicSequence analysisAdd BLAST350

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants are more sensitive to nalidixic acid, mitomycin C and other stresses such as hydrogen peroxide or UV irradiation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002018711 – 387Probable multidrug resistance protein EmrKAdd BLAST387

Proteomic databases

PaxDbiP52599
PRIDEiP52599

Expressioni

Inductioni

Growth phase-dependent transcription is induced by tetracycline. Expression may be controlled by both RpoS and the Mar system.1 Publication

Interactioni

Subunit structurei

Part of the tripartite efflux system EmrYK-TolC, which is composed of an inner membrane transporter, EmrY, a membrane fusion protein, EmrK, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes (By similarity).By similarity

Protein-protein interaction databases

BioGridi4260557, 148 interactors
STRINGi316385.ECDH10B_2533

Structurei

3D structure databases

ProteinModelPortaliP52599
SMRiP52599
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CW7 Bacteria
COG1566 LUCA
HOGENOMiHOG000112072
InParanoidiP52599
KOiK07797
OMAiGRWHEDT
PhylomeDBiP52599

Family and domain databases

InterProiView protein in InterPro
IPR005694 Emr
IPR032317 HlyD_D23
IPR006143 RND_pump_MFP
PfamiView protein in Pfam
PF00529 HlyD, 1 hit
PF16576 HlyD_D23, 1 hit
TIGRFAMsiTIGR00998 8a0101, 1 hit

Sequencei

Sequence statusi: Complete.

P52599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQINSNKKH SNRRKYFSLL AVVLFIAFSG AYAYWSMELE DMISTDDAYV
60 70 80 90 100
TGNADPISAQ VSGSVTVVNH KDTNYVRQGD ILVSLDKTDA TIALNKAKNN
110 120 130 140 150
LANIVRQTNK LYLQDKQYSA EVASARIQYQ QSLEDYNRRV PLAKQGVISK
160 170 180 190 200
ETLEHTKDTL ISSKAALNAA IQAYKANKAL VMNTPLNRQP QVVEAADATK
210 220 230 240 250
EAWLALKRTD IKSPVTGYIA QRSVQVGETV SPGQSLMAVV PARQMWVNAN
260 270 280 290 300
FKETQLTDVR IGQSVNIISD LYGENVVFHG RVTGINMGTG NAFSLLPAQN
310 320 330 340 350
ATGNWIKIVQ RVPVEVSLDP KELMEHPLRI GLSMTATIDT KNEDIAEMPE
360 370 380
LASTVTSMPA YTSKALVIDT SPIEKEISNI ISHNGQL
Length:387
Mass (Da):42,585
Last modified:November 1, 1997 - v2
Checksum:iABAD26F5D68FF38F
GO

Sequence cautioni

The sequence BAA11236 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78168 Genomic DNA Translation: BAA11236.1 Different initiation.
U00096 Genomic DNA Translation: AAC75427.1
AP009048 Genomic DNA Translation: BAA16239.2
PIRiE65010
RefSeqiNP_416869.1, NC_000913.3
WP_000435167.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75427; AAC75427; b2368
BAA16239; BAA16239; BAA16239
GeneIDi946840
KEGGiecj:JW2365
eco:b2368
PATRICifig|1411691.4.peg.4361

Similar proteinsi

Entry informationi

Entry nameiEMRK_ECOLI
AccessioniPrimary (citable) accession number: P52599
Secondary accession number(s): P76517, P76944, P76945
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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