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Protein

Probable multidrug resistance protein EmrK

Gene

emrK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Part of the tripartite efflux system EmrYK-TolC, which confers resistance to various drugs.By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoCyc
  • response to antibiotic Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7233-MONOMER.
ECOL316407:JW2365-MONOMER.
RETL1328306-WGS:GSTH-3726-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable multidrug resistance protein EmrK
Gene namesi
Name:emrK
Ordered Locus Names:b2368, JW2365
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13282. emrK.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence analysisAdd
BLAST
Transmembranei17 – 3721HelicalSequence analysisAdd
BLAST
Topological domaini38 – 387350PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants are more sensitive to nalidixic acid, mitomycin C and other stresses such as hydrogen peroxide or UV irradiation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Probable multidrug resistance protein EmrKPRO_0000201871Add
BLAST

Proteomic databases

PaxDbiP52599.
PRIDEiP52599.

Expressioni

Inductioni

Growth phase-dependent transcription is induced by tetracycline. Expression may be controlled by both RpoS and the Mar system.1 Publication

Interactioni

Subunit structurei

Part of the tripartite efflux system EmrYK-TolC, which is composed of an inner membrane transporter, EmrY, a membrane fusion protein, EmrK, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes (By similarity).By similarity

Protein-protein interaction databases

BioGridi4260557. 148 interactions.
STRINGi511145.b2368.

Structurei

3D structure databases

ProteinModelPortaliP52599.
SMRiP52599. Positions 53-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CW7. Bacteria.
COG1566. LUCA.
HOGENOMiHOG000112072.
InParanoidiP52599.
KOiK07797.
OMAiLDQLWID.
PhylomeDBiP52599.

Family and domain databases

InterProiIPR005694. Emr.
IPR032317. HlyD_D23.
IPR006143. RND_pump_MFP.
[Graphical view]
PfamiPF00529. HlyD. 1 hit.
PF16576. HlyD_D23. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00998. 8a0101. 1 hit.

Sequencei

Sequence statusi: Complete.

P52599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQINSNKKH SNRRKYFSLL AVVLFIAFSG AYAYWSMELE DMISTDDAYV
60 70 80 90 100
TGNADPISAQ VSGSVTVVNH KDTNYVRQGD ILVSLDKTDA TIALNKAKNN
110 120 130 140 150
LANIVRQTNK LYLQDKQYSA EVASARIQYQ QSLEDYNRRV PLAKQGVISK
160 170 180 190 200
ETLEHTKDTL ISSKAALNAA IQAYKANKAL VMNTPLNRQP QVVEAADATK
210 220 230 240 250
EAWLALKRTD IKSPVTGYIA QRSVQVGETV SPGQSLMAVV PARQMWVNAN
260 270 280 290 300
FKETQLTDVR IGQSVNIISD LYGENVVFHG RVTGINMGTG NAFSLLPAQN
310 320 330 340 350
ATGNWIKIVQ RVPVEVSLDP KELMEHPLRI GLSMTATIDT KNEDIAEMPE
360 370 380
LASTVTSMPA YTSKALVIDT SPIEKEISNI ISHNGQL
Length:387
Mass (Da):42,585
Last modified:November 1, 1997 - v2
Checksum:iABAD26F5D68FF38F
GO

Sequence cautioni

The sequence BAA11236 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78168 Genomic DNA. Translation: BAA11236.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75427.1.
AP009048 Genomic DNA. Translation: BAA16239.2.
PIRiE65010.
RefSeqiNP_416869.1. NC_000913.3.
WP_000435167.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75427; AAC75427; b2368.
BAA16239; BAA16239; BAA16239.
GeneIDi946840.
KEGGiecj:JW2365.
eco:b2368.
PATRICi32120115. VBIEscCol129921_2466.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78168 Genomic DNA. Translation: BAA11236.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75427.1.
AP009048 Genomic DNA. Translation: BAA16239.2.
PIRiE65010.
RefSeqiNP_416869.1. NC_000913.3.
WP_000435167.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP52599.
SMRiP52599. Positions 53-352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260557. 148 interactions.
STRINGi511145.b2368.

Proteomic databases

PaxDbiP52599.
PRIDEiP52599.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75427; AAC75427; b2368.
BAA16239; BAA16239; BAA16239.
GeneIDi946840.
KEGGiecj:JW2365.
eco:b2368.
PATRICi32120115. VBIEscCol129921_2466.

Organism-specific databases

EchoBASEiEB3067.
EcoGeneiEG13282. emrK.

Phylogenomic databases

eggNOGiENOG4105CW7. Bacteria.
COG1566. LUCA.
HOGENOMiHOG000112072.
InParanoidiP52599.
KOiK07797.
OMAiLDQLWID.
PhylomeDBiP52599.

Enzyme and pathway databases

BioCyciEcoCyc:G7233-MONOMER.
ECOL316407:JW2365-MONOMER.
RETL1328306-WGS:GSTH-3726-MONOMER.

Miscellaneous databases

PROiP52599.

Family and domain databases

InterProiIPR005694. Emr.
IPR032317. HlyD_D23.
IPR006143. RND_pump_MFP.
[Graphical view]
PfamiPF00529. HlyD. 1 hit.
PF16576. HlyD_D23. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00998. 8a0101. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEMRK_ECOLI
AccessioniPrimary (citable) accession number: P52599
Secondary accession number(s): P76517, P76944, P76945
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.