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Protein

Heterogeneous nuclear ribonucleoprotein F

Gene

HNRNPF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.1 Publication

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • RNA binding Source: ProtInc
  • single-stranded RNA binding Source: UniProtKB

GO - Biological processi

  • fibroblast growth factor receptor signaling pathway Source: Reactome
  • gene expression Source: Reactome
  • mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of RNA splicing Source: UniProtKB
  • RNA processing Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-6803529. FGFR2 alternative splicing.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein F
Short name:
hnRNP F
Alternative name(s):
Nucleolin-like protein mcs94-1
Cleaved into the following chain:
Gene namesi
Name:HNRNPF
Synonyms:HNRPF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:5039. HNRNPF.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • cytoplasm Source: HPA
  • membrane Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi20 – 201W → A: Loss of RNA-binding. 1 Publication
Mutagenesisi84 – 841E → A: Loss of RNA-binding. 1 Publication
Mutagenesisi116 – 1161R → A: Decreases affinity for RNA oligonucleotide 100-fold. 1 Publication
Mutagenesisi120 – 1201F → A: Little disruption of binding RNA. Decreases affinity for RNA oligonucleotide 100-fold. Abrogates RNA-binding; when associated with A-180. 2 Publications
Mutagenesisi150 – 1501K → A: No effect on affinity for RNA oligonucleotide. 1 Publication
Mutagenesisi156 – 1561F → A: Drastically effects folding of RRM2. 1 Publication
Mutagenesisi173 – 1731K → A: Minimal effect on affinity for RNA oligonucleotide. 1 Publication
Mutagenesisi178 – 1781H → A: Little disruption of binding RNA. 1 Publication
Mutagenesisi179 – 1791R → A: Decreases affinity for RNA oligonucleotide 100-fold. 1 Publication
Mutagenesisi180 – 1801Y → A: Decreases affinity for RNA oligonucleotide 10-fold. Abrogates RNA-binding; when associated with A-120. 2 Publications
Mutagenesisi182 – 1821E → A: Decreases affinity for RNA oligonucleotide 100-fold. 1 Publication
Mutagenesisi184 – 1841F → A: Minimal effect on affinity for RNA oligonucleotide. 1 Publication

Organism-specific databases

PharmGKBiPA162391271.

Polymorphism and mutation databases

BioMutaiHNRNPF.
DMDMi1710628.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Heterogeneous nuclear ribonucleoprotein FPRO_0000367114Add
BLAST
Initiator methionineiRemoved; alternateCombined sources1 Publication
Chaini2 – 415414Heterogeneous nuclear ribonucleoprotein F, N-terminally processedPRO_0000081852Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine; in Heterogeneous nuclear ribonucleoprotein F; alternateCombined sources1 Publication
Modified residuei2 – 21N-acetylmethionine; in Heterogeneous nuclear ribonucleoprotein F, N-terminally processedCombined sources1 Publication
Cross-linki72 – 72Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei104 – 1041PhosphoserineCombined sources
Modified residuei107 – 1071PhosphoserineCombined sources
Modified residuei161 – 1611PhosphoserineCombined sources
Modified residuei187 – 1871PhosphoserineCombined sources
Modified residuei193 – 1931PhosphoserineCombined sources
Modified residuei195 – 1951PhosphoserineBy similarity
Modified residuei200 – 2001N6-acetyllysine; alternateBy similarity
Cross-linki200 – 200Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei215 – 2151PhosphothreonineCombined sources
Modified residuei224 – 2241N6-acetyllysineCombined sources
Modified residuei265 – 2651PhosphoserineCombined sources

Post-translational modificationi

Sumoylated.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP52597.
MaxQBiP52597.
PaxDbiP52597.
PeptideAtlasiP52597.
PRIDEiP52597.

2D gel databases

OGPiP52597.

PTM databases

iPTMnetiP52597.
PhosphoSiteiP52597.
SwissPalmiP52597.

Expressioni

Tissue specificityi

Expressed ubiquitously.

Gene expression databases

BgeeiENSG00000169813.
CleanExiHS_HNRNPF.
ExpressionAtlasiP52597. baseline and differential.
GenevisibleiP52597. HS.

Organism-specific databases

HPAiHPA016884.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with AGO1, AGO2, TBP and TXNL4/DIM1.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei16 – 161Interaction with RNA
Sitei20 – 201Interaction with RNA
Sitei52 – 521Interaction with RNA
Sitei75 – 751Interaction with RNA
Sitei116 – 1161Interaction with RNA
Sitei120 – 1201Interaction with RNA
Sitei150 – 1501Interaction with RNA
Sitei173 – 1731Interaction with RNA
Sitei294 – 2941Interaction with RNA
Sitei298 – 2981Interaction with RNA
Sitei326 – 3261Interaction with RNA
Sitei349 – 3491Interaction with RNA

Binary interactionsi

WithEntry#Exp.IntActNotes
AESQ081173EBI-352986,EBI-717810
C1orf94Q6P1W53EBI-352986,EBI-946029
DZIP3Q86Y133EBI-352986,EBI-948630
EIF4ENIF1Q9NRA83EBI-352986,EBI-301024
HNRNPMP522723EBI-352986,EBI-486809
HNRNPUL1Q9BUJ24EBI-352986,EBI-1018153
HSFY2Q96LI63EBI-352986,EBI-3957665
IKZF3Q9UKT93EBI-352986,EBI-747204
RBFOX2O432514EBI-352986,EBI-746056
TFGQ927343EBI-352986,EBI-357061

Protein-protein interaction databases

BioGridi109426. 173 interactions.
DIPiDIP-33145N.
IntActiP52597. 75 interactions.
MINTiMINT-1157890.
STRINGi9606.ENSP00000338477.

Structurei

Secondary structure

1
415
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 176Combined sources
Helixi24 – 307Combined sources
Turni31 – 333Combined sources
Beta strandi37 – 404Combined sources
Beta strandi43 – 475Combined sources
Beta strandi49 – 513Combined sources
Beta strandi53 – 608Combined sources
Helixi64 – 718Combined sources
Turni72 – 743Combined sources
Beta strandi75 – 8713Combined sources
Helixi90 – 978Combined sources
Beta strandi107 – 1093Combined sources
Beta strandi112 – 1165Combined sources
Helixi124 – 1307Combined sources
Turni131 – 1333Combined sources
Beta strandi136 – 1427Combined sources
Beta strandi147 – 1515Combined sources
Beta strandi153 – 1619Combined sources
Helixi164 – 1696Combined sources
Turni170 – 1734Combined sources
Beta strandi175 – 1806Combined sources
Beta strandi184 – 1863Combined sources
Helixi188 – 1914Combined sources
Beta strandi284 – 29411Combined sources
Helixi302 – 3098Combined sources
Beta strandi315 – 3184Combined sources
Beta strandi322 – 3254Combined sources
Beta strandi332 – 3343Combined sources
Helixi337 – 3448Combined sources
Beta strandi351 – 3544Combined sources
Beta strandi358 – 3625Combined sources
Beta strandi368 – 3714Combined sources
Beta strandi377 – 3793Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HGLNMR-A1-102[»]
2HGMNMR-A103-194[»]
2HGNNMR-A277-381[»]
2KFYNMR-A1-102[»]
2KG0NMR-A103-194[»]
2KG1NMR-A277-381[»]
3TFYX-ray2.75D/E/F2-10[»]
ProteinModelPortaliP52597.
SMRiP52597. Positions 1-194, 277-381.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52597.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 8573RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini111 – 18878RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini289 – 36678RRM 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni81 – 866Interaction with RNA
Regioni179 – 1846Interaction with RNA
Regioni355 – 3606Interaction with RNA

Domaini

The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4211. Eukaryota.
ENOG410Z6M0. LUCA.
GeneTreeiENSGT00760000119102.
HOGENOMiHOG000220896.
HOVERGENiHBG055557.
InParanoidiP52597.
KOiK12898.
OMAiFLSECKI.
OrthoDBiEOG091G0CTJ.
PhylomeDBiP52597.
TreeFamiTF316157.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR012996. Znf_CHHC.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
PF08080. zf-RNPHF. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52597-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLGPEGGEG FVVKLRGLPW SCSVEDVQNF LSDCTIHDGA AGVHFIYTRE
60 70 80 90 100
GRQSGEAFVE LGSEDDVKMA LKKDRESMGH RYIEVFKSHR TEMDWVLKHS
110 120 130 140 150
GPNSADSAND GFVRLRGLPF GCTKEEIVQF FSGLEIVPNG ITLPVDPEGK
160 170 180 190 200
ITGEAFVQFA SQELAEKALG KHKERIGHRY IEVFKSSQEE VRSYSDPPLK
210 220 230 240 250
FMSVQRPGPY DRPGTARRYI GIVKQAGLER MRPGAYSTGY GGYEEYSGLS
260 270 280 290 300
DGYGFTTDLF GRDLSYCLSG MYDHRYGDSE FTVQSTTGHC VHMRGLPYKA
310 320 330 340 350
TENDIYNFFS PLNPVRVHIE IGPDGRVTGE ADVEFATHEE AVAAMSKDRA
360 370 380 390 400
NMQHRYIELF LNSTTGASNG AYSSQVMQGM GVSAAQATYS GLESQSVSGC
410
YGAGYSGQNS MGGYD
Length:415
Mass (Da):45,672
Last modified:January 23, 2007 - v3
Checksum:iD14E170631FB1F31
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti87 – 871K → R.1 Publication
Corresponds to variant rs17851426 [ dbSNP | Ensembl ].
VAR_027999

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28010 mRNA. Translation: AAC37584.1.
AL512654 Genomic DNA. Translation: CAI17066.1.
AK001364 mRNA. Translation: BAG50896.1.
CH471160 Genomic DNA. Translation: EAW86595.1.
BC001432 mRNA. Translation: AAH01432.1.
BC004254 mRNA. Translation: AAH04254.1.
BC015580 mRNA. Translation: AAH15580.1.
BC016736 mRNA. Translation: AAH16736.1.
BC106008 mRNA. Translation: AAI06009.1.
CCDSiCCDS7204.1.
PIRiS43484.
RefSeqiNP_001091674.1. NM_001098204.1.
NP_001091675.1. NM_001098205.1.
NP_001091676.1. NM_001098206.1.
NP_001091677.1. NM_001098207.1.
NP_001091678.1. NM_001098208.1.
NP_004957.1. NM_004966.3.
UniGeneiHs.712955.
Hs.808.

Genome annotation databases

EnsembliENST00000337970; ENSP00000338477; ENSG00000169813.
ENST00000356053; ENSP00000348345; ENSG00000169813.
ENST00000357065; ENSP00000349573; ENSG00000169813.
ENST00000443950; ENSP00000400433; ENSG00000169813.
ENST00000544000; ENSP00000438061; ENSG00000169813.
GeneIDi3185.
KEGGihsa:3185.
UCSCiuc001jar.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28010 mRNA. Translation: AAC37584.1.
AL512654 Genomic DNA. Translation: CAI17066.1.
AK001364 mRNA. Translation: BAG50896.1.
CH471160 Genomic DNA. Translation: EAW86595.1.
BC001432 mRNA. Translation: AAH01432.1.
BC004254 mRNA. Translation: AAH04254.1.
BC015580 mRNA. Translation: AAH15580.1.
BC016736 mRNA. Translation: AAH16736.1.
BC106008 mRNA. Translation: AAI06009.1.
CCDSiCCDS7204.1.
PIRiS43484.
RefSeqiNP_001091674.1. NM_001098204.1.
NP_001091675.1. NM_001098205.1.
NP_001091676.1. NM_001098206.1.
NP_001091677.1. NM_001098207.1.
NP_001091678.1. NM_001098208.1.
NP_004957.1. NM_004966.3.
UniGeneiHs.712955.
Hs.808.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HGLNMR-A1-102[»]
2HGMNMR-A103-194[»]
2HGNNMR-A277-381[»]
2KFYNMR-A1-102[»]
2KG0NMR-A103-194[»]
2KG1NMR-A277-381[»]
3TFYX-ray2.75D/E/F2-10[»]
ProteinModelPortaliP52597.
SMRiP52597. Positions 1-194, 277-381.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109426. 173 interactions.
DIPiDIP-33145N.
IntActiP52597. 75 interactions.
MINTiMINT-1157890.
STRINGi9606.ENSP00000338477.

PTM databases

iPTMnetiP52597.
PhosphoSiteiP52597.
SwissPalmiP52597.

Polymorphism and mutation databases

BioMutaiHNRNPF.
DMDMi1710628.

2D gel databases

OGPiP52597.

Proteomic databases

EPDiP52597.
MaxQBiP52597.
PaxDbiP52597.
PeptideAtlasiP52597.
PRIDEiP52597.

Protocols and materials databases

DNASUi3185.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337970; ENSP00000338477; ENSG00000169813.
ENST00000356053; ENSP00000348345; ENSG00000169813.
ENST00000357065; ENSP00000349573; ENSG00000169813.
ENST00000443950; ENSP00000400433; ENSG00000169813.
ENST00000544000; ENSP00000438061; ENSG00000169813.
GeneIDi3185.
KEGGihsa:3185.
UCSCiuc001jar.2. human.

Organism-specific databases

CTDi3185.
GeneCardsiHNRNPF.
HGNCiHGNC:5039. HNRNPF.
HPAiHPA016884.
MIMi601037. gene.
neXtProtiNX_P52597.
PharmGKBiPA162391271.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4211. Eukaryota.
ENOG410Z6M0. LUCA.
GeneTreeiENSGT00760000119102.
HOGENOMiHOG000220896.
HOVERGENiHBG055557.
InParanoidiP52597.
KOiK12898.
OMAiFLSECKI.
OrthoDBiEOG091G0CTJ.
PhylomeDBiP52597.
TreeFamiTF316157.

Enzyme and pathway databases

ReactomeiR-HSA-6803529. FGFR2 alternative splicing.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.

Miscellaneous databases

ChiTaRSiHNRNPF. human.
EvolutionaryTraceiP52597.
GeneWikiiHNRPF.
GenomeRNAii3185.
PROiP52597.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169813.
CleanExiHS_HNRNPF.
ExpressionAtlasiP52597. baseline and differential.
GenevisibleiP52597. HS.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR012996. Znf_CHHC.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
PF08080. zf-RNPHF. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNRPF_HUMAN
AccessioniPrimary (citable) accession number: P52597
Secondary accession number(s): B3KM84, Q5T0N2, Q96AU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 184 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.