Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arf-GAP domain and FG repeat-containing protein 1

Gene

AGFG1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri29 – 52C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • GTPase activator activity Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDifferentiation, mRNA transport, Spermatogenesis, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-HSA-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP domain and FG repeat-containing protein 1
Alternative name(s):
HIV-1 Rev-binding protein
Nucleoporin-like protein RIP
Rev-interacting protein
Rev/Rex activation domain-binding protein
Gene namesi
Name:AGFG1
Synonyms:HRB, RAB, RIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:5175. AGFG1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasmic vesicle, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3267.
OpenTargetsiENSG00000173744.
PharmGKBiPA29449.

Polymorphism and mutation databases

BioMutaiAGFG1.
DMDMi26007019.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002049041 – 562Arf-GAP domain and FG repeat-containing protein 1Add BLAST562

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei167PhosphoserineCombined sources1
Modified residuei177PhosphothreonineCombined sources1
Modified residuei181PhosphoserineCombined sources1
Modified residuei362PhosphoserineCombined sources1
Glycosylationi367O-linked (GlcNAc) serineBy similarity1

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP52594.
MaxQBiP52594.
PaxDbiP52594.
PeptideAtlasiP52594.
PRIDEiP52594.

PTM databases

iPTMnetiP52594.
PhosphoSitePlusiP52594.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000173744.
CleanExiHS_AGFG1.
ExpressionAtlasiP52594. baseline and differential.
GenevisibleiP52594. HS.

Organism-specific databases

HPAiHPA008741.

Interactioni

Subunit structurei

Interacts with EPS15R and EPS15. Interacts with FCHO1.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi109503. 41 interactors.
DIPiDIP-35546N.
ELMiP52594.
IntActiP52594. 9 interactors.
MINTiMINT-1651458.
STRINGi9606.ENSP00000387282.

Structurei

Secondary structure

1562
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 21Combined sources18
Helixi24 – 27Combined sources4
Turni30 – 32Combined sources3
Beta strandi39 – 41Combined sources3
Turni42 – 45Combined sources4
Beta strandi46 – 48Combined sources3
Helixi50 – 56Combined sources7
Beta strandi59 – 61Combined sources3
Beta strandi65 – 67Combined sources3
Turni68 – 70Combined sources3
Helixi75 – 83Combined sources9
Helixi85 – 93Combined sources9
Turni94 – 96Combined sources3
Turni99 – 101Combined sources3
Helixi110 – 121Combined sources12
Helixi130 – 134Combined sources5
Beta strandi157 – 159Combined sources3
Helixi160 – 164Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D9LNMR-A14-134[»]
2OLMX-ray1.48A4-141[»]
2VX8X-ray2.20A/B/C/D136-175[»]
ProteinModelPortaliP52594.
SMRiP52594.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52594.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 135Arf-GAPPROSITE-ProRule annotationAdd BLAST125

Domaini

Contains FG repeats. The FG repeat region is required for acting as a cofactor of HIV-1 Rev.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri29 – 52C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IUMA. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00560000077249.
HOGENOMiHOG000253009.
HOVERGENiHBG006551.
InParanoidiP52594.
KOiK15044.
OMAiHFNSHAA.
OrthoDBiEOG091G09LC.
PhylomeDBiP52594.
TreeFamiTF325357.

Family and domain databases

InterProiView protein in InterPro
IPR001164. ArfGAP.
PfamiView protein in Pfam
PF01412. ArfGap. 1 hit.
PRINTSiPR00405. REVINTRACTNG.
SMARTiView protein in SMART
SM00105. ArfGap. 1 hit.
PROSITEiView protein in PROSITE
PS50115. ARFGAP. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P52594-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASAKRKQE EKHLKMLRDM TGLPHNRKCF DCDQRGPTYV NMTVGSFVCT
60 70 80 90 100
SCSGSLRGLN PPHRVKSISM TTFTQQEIEF LQKHGNEVCK QIWLGLFDDR
110 120 130 140 150
SSAIPDFRDP QKVKEFLQEK YEKKRWYVPP EQAKVVASVH ASISGSSASS
160 170 180 190 200
TSSTPEVKPL KSLLGDSAPT LHLNKGTPSQ SPVVGRSQGQ QQEKKQFDLL
210 220 230 240 250
SDLGSDIFAA PAPQSTATAN FANFAHFNSH AAQNSANADF ANFDAFGQSS
260 270 280 290 300
GSSNFGGFPT ASHSPFQPQT TGGSAASVNA NFAHFDNFPK SSSADFGTFN
310 320 330 340 350
TSQSHQTASA VSKVSTNKAG LQTADKYAAL ANLDNIFSAG QGGDQGSGFG
360 370 380 390 400
TTGKAPVGSV VSVPSQSSAS SDKYAALAEL DSVFSSAATS SNAYTSTSNA
410 420 430 440 450
SSNVFGTVPV VASAQTQPAS SSVPAPFGAT PSTNPFVAAA GPSVASSTNP
460 470 480 490 500
FQTNARGATA ATFGTASMSM PTGFGTPAPY SLPTSFSGSF QQPAFPAQAA
510 520 530 540 550
FPQQTAFSQQ PNGAGFAAFG QTKPVVTPFG QVAAAGVSSN PFMTGAPTGQ
560
FPTGSSSTNP FL
Length:562
Mass (Da):58,260
Last modified:November 28, 2002 - v2
Checksum:i9A2242217F53B4D9
GO
Isoform 2 (identifier: P52594-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     232-271: Missing.

Show »
Length:522
Mass (Da):54,182
Checksum:iDE779347F13D2557
GO
Isoform 3 (identifier: P52594-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     513-514: Missing.

Show »
Length:560
Mass (Da):58,132
Checksum:iAE7E67C63C6830ED
GO
Isoform 4 (identifier: P52594-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     272-272: G → AFRMLSSSCSFGEFTSAFPLQATHS
     513-514: Missing.

Note: No experimental confirmation available.
Show »
Length:584
Mass (Da):60,767
Checksum:i4D508894C0FFE3F6
GO

Sequence cautioni

The sequence BAG53473 differs from that shown. Aberrant splicing within exon 1.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25H → R in CAA61667 (PubMed:7637788).Curated1
Sequence conflicti429A → R in CAA61667 (PubMed:7637788).Curated1
Sequence conflicti542F → S in BAG53473 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017600232 – 271Missing in isoform 2. 1 PublicationAdd BLAST40
Alternative sequenceiVSP_046186272G → AFRMLSSSCSFGEFTSAFPL QATHS in isoform 4. 1 Publication1
Alternative sequenceiVSP_017601513 – 514Missing in isoform 3 and isoform 4. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42025 Genomic DNA. Translation: AAC37580.1.
X89478 mRNA. Translation: CAA61667.1.
AK097451 mRNA. Translation: BAG53473.1. Sequence problems.
AC105286 Genomic DNA. Translation: AAX93270.1.
AC097662 Genomic DNA. Translation: AAY24254.1.
BC030592 mRNA. Translation: AAH30592.1.
BC096272 mRNA. Translation: AAH96272.1.
BC096273 mRNA. Translation: AAH96273.1.
BC096274 mRNA. Translation: AAH96274.1.
BC096275 mRNA. Translation: AAH96275.1.
CCDSiCCDS2467.1. [P52594-1]
CCDS46533.1. [P52594-4]
CCDS46534.1. [P52594-3]
CCDS46535.1. [P52594-2]
PIRiA57088.
RefSeqiNP_001128659.1. NM_001135187.1. [P52594-4]
NP_001128660.1. NM_001135188.1. [P52594-3]
NP_001128661.1. NM_001135189.1. [P52594-2]
NP_004495.2. NM_004504.4. [P52594-1]
UniGeneiHs.352962.

Genome annotation databases

EnsembliENST00000310078; ENSP00000312059; ENSG00000173744. [P52594-1]
ENST00000373671; ENSP00000362775; ENSG00000173744. [P52594-2]
ENST00000409171; ENSP00000387218; ENSG00000173744. [P52594-3]
ENST00000409979; ENSP00000387282; ENSG00000173744. [P52594-4]
GeneIDi3267.
KEGGihsa:3267.
UCSCiuc002vpc.3. human. [P52594-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAGFG1_HUMAN
AccessioniPrimary (citable) accession number: P52594
Secondary accession number(s): B3KUL1
, E9PHX7, Q15277, Q4VAS0, Q4VAS1, Q4VAS3, Q53QT8, Q53R11
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 28, 2002
Last modified: August 30, 2017
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references