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P52593 (NU188_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nucleoporin NUP188
Alternative name(s):
Nuclear pore protein NUP188
Gene names
Name:NUP188
Ordered Locus Names:YML103C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1655 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP188 probably plays an important role in NPC assembly and organization.

Subunit structure

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Interacts with POM152 and NIC96. Ref.1 Ref.2 Ref.6

Subcellular location

Nucleusnuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Symmetric distribution.

Miscellaneous

Present with 11700 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 16551655Nucleoporin NUP188
PRO_0000204856

Regions

Region250 – 27122Leucine-zipper
Coiled coil35 – 6228 Potential
Compositional bias485 – 4895Poly-Ser
Compositional bias533 – 54513Gln-rich
Compositional bias533 – 5419Poly-Gln
Compositional bias1385 – 13917Poly-Leu
Compositional bias1504 – 15074Poly-Leu

Amino acid modifications

Modified residue3401Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P52593 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B590051EEE60464C

FASTA1,655188,577
        10         20         30         40         50         60 
MATPSFGNSS PQLTFTHVAN FMNDAAADVS AVDAKQLAQI RQFLKANKTN LIESLNTIRQ 

        70         80         90        100        110        120 
NVTSSGDHNK LRSTIANLLQ INVDNDPFFA QSEDLSHAVE FFMSERSSRL HIVYSLLVNP 

       130        140        150        160        170        180 
DIDLETYSFI DNDRFNVVGK LISIISSVIQ NYDIITASSL AHDYNNDQDM FTIVSLVQLK 

       190        200        210        220        230        240 
KFSDLKFILQ ILQILNLMIL NTKVPVDIVN QWFLQYQNQF VEFCRNINST DKSIDTSSLQ 

       250        260        270        280        290        300 
LYKFQNFQDL SYLSETLISR ISSLFTITTI LILGLNTSIA QFDIQSPLYM DTETFDTVNS 

       310        320        330        340        350        360 
ALENDVATNI VNEDPIFHPM IHYSWSFILY YRRALQSSES FDDSDITKFA LFAESHDVLQ 

       370        380        390        400        410        420 
KLNTLSEILS FDPVYTTVIT VFLEFSLNFI PITASTSRVF AKIISKAPEQ FIENFLTNDT 

       430        440        450        460        470        480 
FEKKLSIIKA KLPLLNESLI PLINLALIDT EFANFELKDI CSFAVTKSSL NDLDYDLIAD 

       490        500        510        520        530        540 
TITNSSSSSD IIVPDLIELK SDLLVAPPLE NENSNCLLSI PKSTKGKILT IKQQQQQQQQ 

       550        560        570        580        590        600 
QNGQQPPTTS NLIIFLYKFN GWSLVGRILQ NLLHSYMEKG TQLDDLQHEL MISIIKLVTN 

       610        620        630        640        650        660 
VVDPKTSIEK SSEILSYLSN SLDTSASTIN GASIIQVIFE IFEISLQRKD YTSIVQCCEF 

       670        680        690        700        710        720 
MTMLTPNYLH LVSSYLNKSD LLDKYGKTGL SNMILGSVEL STGDYTFTIQ LLKLTKVFIR 

       730        740        750        760        770        780 
ESLSLKNIHI SKRSKIDIIN KLILHAIHIF ESYYNWKYNN FLQKFEIAFH LTLIFYDVLH 

       790        800        810        820        830        840 
DVFTINPHQK DQLIISSSAN KLLQLFLTPM DSIDLAPNTL TNILISPLNT TTKILGDKIL 

       850        860        870        880        890        900 
GNLYSKVMNN SFKLCTLLIA IRGSNRDLKP SNLEKLLFIN SSKLVDVYTL PSYVHFKVQI 

       910        920        930        940        950        960 
IELLSYLVEA PWNDDYPFLL SFLGEAKSMA FLKEVLSDLS SPVQDWNLLR SLYIFFTTLL 

       970        980        990       1000       1010       1020 
ESKQDGLSIL FLTGQFASNK KINDESSIDK KSSILTVLQK NSLLLDSTPE EVSCKLLETI 

      1030       1040       1050       1060       1070       1080 
TYVLNTWTNS KIFIKDPKFV NSLLAKLKDS KKLFQKKENL TRDETVSLIK KYKLISRIVE 

      1090       1100       1110       1120       1130       1140 
IFALCIYNST DSNSEILNFL NQEDLFELVH HFFQIDGFNK TFHDELNLKF KEKWPSLELQ 

      1150       1160       1170       1180       1190       1200 
SFQKIPLSRI NENENFGYDI PLLDIVLKAD RSWNEPSKSQ TNFKEEITDA SLNLQYVNYE 

      1210       1220       1230       1240       1250       1260 
ISTAKAWGAL ITTFVKRSTV PLNDGFVDLV EHFLKLNIDF GSDKQMFTQI YLERIELSFY 

      1270       1280       1290       1300       1310       1320 
ILYSFKLSGK LLKEEKIIEL MNKIFTIFKS GEIDFIKNIG KSLKNNFYRP LLRSVLVLLE 

      1330       1340       1350       1360       1370       1380 
LVSSGDRFIE LISDQLLEFF ELVFSKGVYL ILSEILCQIN KCSTRGLSTD HTTQIVNLED 

      1390       1400       1410       1420       1430       1440 
NTQDLLLLLS LFKKITNVNP SKNFNVILAS SLNEVGTLKV ILNLYSSAHL IRINDEPILG 

      1450       1460       1470       1480       1490       1500 
QITLTFISEL CSIEPIAAKL INSGLYSVLL ESPLSVAIQQ GDIKPEFSPR LHNIWSNGLL 

      1510       1520       1530       1540       1550       1560 
SIVLLLLSQF GIKVLPETCL FVSYFGKQIK STIYNWGDNK LAVSSSLIKE TNQLVLLQKM 

      1570       1580       1590       1600       1610       1620 
LNLLNYQELF IQPKNSDDQQ EAVELVIGLD SEHDKKRLSA ALSKFLTHPK YLNSRIIPTT 

      1630       1640       1650 
LEEQQQLEDE SSRLEFVKGI SRDIKALQDS LFKDV 

« Hide

References

« Hide 'large scale' references
[1]"Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p."
Zabel U., Doye V., Tekotte H., Wepf R., Grandi P., Hurt E.C.
J. Cell Biol. 133:1141-1152(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH NIC96.
[2]"The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex."
Nehrbass U., Rout M.P., Maguire S., Blobel G., Wozniak R.W.
J. Cell Biol. 133:1153-1162(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH NIC96 AND POM152.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p."
Aitchison J.D., Rout M.P., Marelli M., Blobel G., Wozniak R.W.
J. Cell Biol. 131:1133-1148(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1187-1205 AND 1611-1629.
[6]"The yeast nuclear pore complex: composition, architecture, and transport mechanism."
Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
[7]"Peering through the pore: nuclear pore complex structure, assembly, and function."
Suntharalingam M., Wente S.R.
Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-340, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X90580 Genomic DNA. Translation: CAA62208.1.
U47107 Genomic DNA. Translation: AAA88904.1.
X80835 Genomic DNA. Translation: CAA56794.1.
BK006946 Genomic DNA. Translation: DAA09795.1.
PIRS47446.
RefSeqNP_013604.1. NM_001182465.1.

3D structure databases

ProteinModelPortalP52593.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35040. 223 interactions.
DIPDIP-2429N.
IntActP52593. 27 interactions.
MINTMINT-2780953.
STRING4932.YML103C.

Protein family/group databases

TCDB1.I.1.1.1. the nuclear pore complex (npc) family.

Proteomic databases

MaxQBP52593.
PaxDbP52593.
PeptideAtlasP52593.

Protocols and materials databases

DNASU854868.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML103C; YML103C; YML103C.
GeneID854868.
KEGGsce:YML103C.

Organism-specific databases

CYGDYML103c.
SGDS000004571. NUP188.

Phylogenomic databases

eggNOGNOG68960.
HOGENOMHOG000113877.
KOK14311.
OMADFICAVM.
OrthoDBEOG7JDR69.

Enzyme and pathway databases

BioCycYEAST:G3O-32687-MONOMER.

Gene expression databases

GenevestigatorP52593.

Family and domain databases

InterProIPR018864. Nucleoporin_Nup188.
[Graphical view]
PfamPF10487. Nup188. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977798.

Entry information

Entry nameNU188_YEAST
AccessionPrimary (citable) accession number: P52593
Secondary accession number(s): D6W0I1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD