P52593 (NU188_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoporin NUP188

Alternative name(s):
Nuclear pore protein NUP188

Gene names

Name:	NUP188
Ordered Locus Names:	YML103C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1655 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP188 probably plays an important role in NPC assembly and organization.
Subunit structure	The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Interacts with POM152 and NIC96. Ref.1 Ref.2 Ref.6
Subcellular location	Nucleus › nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Symmetric distribution.
Miscellaneous	Present with 11700 molecules/cell in log phase SD medium. Ref.8

Ontologies

Keywords
Biological process	Protein transport Translocation Transport mRNA transport
Cellular component	Membrane Nuclear pore complex Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	NLS-bearing substrate import into nucleus Traceable author statement. Source: SGD mRNA export from nucleus in response to heat stress Inferred from mutant phenotype. Source: SGD mRNA-binding (hnRNP) protein import into nucleus Traceable author statement. Source: SGD nuclear pore organization Inferred from mutant phenotype. Source: SGD protein export from nucleus Traceable author statement. Source: SGD rRNA export from nucleus Traceable author statement. Source: SGD ribosomal protein import into nucleus Traceable author statement. Source: SGD snRNA export from nucleus Traceable author statement. Source: SGD snRNP protein import into nucleus Traceable author statement. Source: SGD tRNA export from nucleus Traceable author statement. Source: SGD
Cellular component	nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear pore Inferred from direct assay Ref.6. Source: SGD
Molecular function	structural molecule activity Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1655	1655	Nucleoporin NUP188		PRO_0000204856
Regions
	Domain	250 – 271	22	Leucine-zipper
	Coiled coil	35 – 62	28	Potential
	Compositional bias	485 – 489	5	Poly-Ser
	Compositional bias	533 – 545	13	Gln-rich
	Compositional bias	533 – 541	9	Poly-Gln
	Compositional bias	1385 – 1391	7	Poly-Leu
	Compositional bias	1504 – 1507	4	Poly-Leu
Amino acid modifications
	Modified residue	237	1	Phosphoserine Ref.9
	Modified residue	340	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P52593 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B590051EEE60464C	FASTA	1,655	188,577
	10 20 30 40 50 60 MATPSFGNSS PQLTFTHVAN FMNDAAADVS AVDAKQLAQI RQFLKANKTN LIESLNTIRQ 70 80 90 100 110 120 NVTSSGDHNK LRSTIANLLQ INVDNDPFFA QSEDLSHAVE FFMSERSSRL HIVYSLLVNP 130 140 150 160 170 180 DIDLETYSFI DNDRFNVVGK LISIISSVIQ NYDIITASSL AHDYNNDQDM FTIVSLVQLK 190 200 210 220 230 240 KFSDLKFILQ ILQILNLMIL NTKVPVDIVN QWFLQYQNQF VEFCRNINST DKSIDTSSLQ 250 260 270 280 290 300 LYKFQNFQDL SYLSETLISR ISSLFTITTI LILGLNTSIA QFDIQSPLYM DTETFDTVNS 310 320 330 340 350 360 ALENDVATNI VNEDPIFHPM IHYSWSFILY YRRALQSSES FDDSDITKFA LFAESHDVLQ 370 380 390 400 410 420 KLNTLSEILS FDPVYTTVIT VFLEFSLNFI PITASTSRVF AKIISKAPEQ FIENFLTNDT 430 440 450 460 470 480 FEKKLSIIKA KLPLLNESLI PLINLALIDT EFANFELKDI CSFAVTKSSL NDLDYDLIAD 490 500 510 520 530 540 TITNSSSSSD IIVPDLIELK SDLLVAPPLE NENSNCLLSI PKSTKGKILT IKQQQQQQQQ 550 560 570 580 590 600 QNGQQPPTTS NLIIFLYKFN GWSLVGRILQ NLLHSYMEKG TQLDDLQHEL MISIIKLVTN 610 620 630 640 650 660 VVDPKTSIEK SSEILSYLSN SLDTSASTIN GASIIQVIFE IFEISLQRKD YTSIVQCCEF 670 680 690 700 710 720 MTMLTPNYLH LVSSYLNKSD LLDKYGKTGL SNMILGSVEL STGDYTFTIQ LLKLTKVFIR 730 740 750 760 770 780 ESLSLKNIHI SKRSKIDIIN KLILHAIHIF ESYYNWKYNN FLQKFEIAFH LTLIFYDVLH 790 800 810 820 830 840 DVFTINPHQK DQLIISSSAN KLLQLFLTPM DSIDLAPNTL TNILISPLNT TTKILGDKIL 850 860 870 880 890 900 GNLYSKVMNN SFKLCTLLIA IRGSNRDLKP SNLEKLLFIN SSKLVDVYTL PSYVHFKVQI 910 920 930 940 950 960 IELLSYLVEA PWNDDYPFLL SFLGEAKSMA FLKEVLSDLS SPVQDWNLLR SLYIFFTTLL 970 980 990 1000 1010 1020 ESKQDGLSIL FLTGQFASNK KINDESSIDK KSSILTVLQK NSLLLDSTPE EVSCKLLETI 1030 1040 1050 1060 1070 1080 TYVLNTWTNS KIFIKDPKFV NSLLAKLKDS KKLFQKKENL TRDETVSLIK KYKLISRIVE 1090 1100 1110 1120 1130 1140 IFALCIYNST DSNSEILNFL NQEDLFELVH HFFQIDGFNK TFHDELNLKF KEKWPSLELQ 1150 1160 1170 1180 1190 1200 SFQKIPLSRI NENENFGYDI PLLDIVLKAD RSWNEPSKSQ TNFKEEITDA SLNLQYVNYE 1210 1220 1230 1240 1250 1260 ISTAKAWGAL ITTFVKRSTV PLNDGFVDLV EHFLKLNIDF GSDKQMFTQI YLERIELSFY 1270 1280 1290 1300 1310 1320 ILYSFKLSGK LLKEEKIIEL MNKIFTIFKS GEIDFIKNIG KSLKNNFYRP LLRSVLVLLE 1330 1340 1350 1360 1370 1380 LVSSGDRFIE LISDQLLEFF ELVFSKGVYL ILSEILCQIN KCSTRGLSTD HTTQIVNLED 1390 1400 1410 1420 1430 1440 NTQDLLLLLS LFKKITNVNP SKNFNVILAS SLNEVGTLKV ILNLYSSAHL IRINDEPILG 1450 1460 1470 1480 1490 1500 QITLTFISEL CSIEPIAAKL INSGLYSVLL ESPLSVAIQQ GDIKPEFSPR LHNIWSNGLL 1510 1520 1530 1540 1550 1560 SIVLLLLSQF GIKVLPETCL FVSYFGKQIK STIYNWGDNK LAVSSSLIKE TNQLVLLQKM 1570 1580 1590 1600 1610 1620 LNLLNYQELF IQPKNSDDQQ EAVELVIGLD SEHDKKRLSA ALSKFLTHPK YLNSRIIPTT 1630 1640 1650 LEEQQQLEDE SSRLEFVKGI SRDIKALQDS LFKDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p." Zabel U., Doye V., Tekotte H., Wepf R., Grandi P., Hurt E.C. J. Cell Biol. 133:1141-1152(1996) [PubMed: 8682854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH NIC96.
[2]	"The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex." Nehrbass U., Rout M.P., Maguire S., Blobel G., Wozniak R.W. J. Cell Biol. 133:1153-1162(1996) [PubMed: 8682855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH NIC96 AND POM152.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p." Aitchison J.D., Rout M.P., Marelli M., Blobel G., Wozniak R.W. J. Cell Biol. 131:1133-1148(1995) [PubMed: 8522578] [Abstract] Cited for: PROTEIN SEQUENCE OF 1187-1205 AND 1611-1629.
[6]	"The yeast nuclear pore complex: composition, architecture, and transport mechanism." Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T. J. Cell Biol. 148:635-651(2000) [PubMed: 10684247] [Abstract] Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
[7]	"Peering through the pore: nuclear pore complex structure, assembly, and function." Suntharalingam M., Wente S.R. Dev. Cell 4:775-789(2003) [PubMed: 12791264] [Abstract] Cited for: REVIEW.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-237 AND SER-340, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X90580 Genomic DNA. Translation: CAA62208.1. U47107 Genomic DNA. Translation: AAA88904.1. X80835 Genomic DNA. Translation: CAA56794.1. BK006946 Genomic DNA. Translation: DAA09795.1.
PIR	S47446.
RefSeq	NP_013604.1. NM_001182465.1.
3D structure databases
ProteinModelPortal	P52593.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2429N.
IntAct	P52593. 28 interactions.
MINT	MINT-2780953.
STRING	P52593.
Protein family/group databases
TCDB	9.A.14.1.1. nuclear pore complex (NPC) family.
Proteomic databases
PeptideAtlas	P52593.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML103C; YML103C; YML103C.
GeneID	854868.
KEGG	sce:YML103C.
NMPDR	fig|4932.3.peg.4640.
Organism-specific databases
CYGD	YML103c.
SGD	S000004571. NUP188.
Phylogenomic databases
eggNOG	fuNOG04806.
GeneTree	EFGT00050000005049.
HOGENOM	HBG203462.
OMA	YDFICAV.
OrthoDB	EOG483HCM.
Gene expression databases
ArrayExpress	P52593.
Genevestigator	P52593.
GermOnline	YML103C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018864. Nucleoporin_Nup188. [Graphical view]
KO	K14311.
Pfam	PF10487. Nup188. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977798.

Entry information

Entry name

NU188_YEAST

Accession

Primary (citable) accession number: P52593
Secondary accession number(s): D6W0I1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program