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P52593

- NU188_YEAST

UniProt

P52593 - NU188_YEAST

Protein

Nucleoporin NUP188

Gene

NUP188

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP188 probably plays an important role in NPC assembly and organization.

    GO - Molecular functioni

    1. structural constituent of nuclear pore Source: SGD

    GO - Biological processi

    1. mRNA export from nucleus in response to heat stress Source: SGD
    2. nuclear pore organization Source: SGD
    3. protein import into nucleus Source: SGD

    Keywords - Biological processi

    mRNA transport, Protein transport, Translocation, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32687-MONOMER.

    Protein family/group databases

    TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoporin NUP188
    Alternative name(s):
    Nuclear pore protein NUP188
    Gene namesi
    Name:NUP188
    Ordered Locus Names:YML103C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML103c.
    SGDiS000004571. NUP188.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear membrane Source: UniProtKB-SubCell
    2. nuclear pore Source: SGD
    3. nuclear pore inner ring Source: SGD

    Keywords - Cellular componenti

    Membrane, Nuclear pore complex, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 16551655Nucleoporin NUP188PRO_0000204856Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei340 – 3401Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP52593.
    PaxDbiP52593.
    PeptideAtlasiP52593.

    Expressioni

    Gene expression databases

    GenevestigatoriP52593.

    Interactioni

    Subunit structurei

    The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Interacts with POM152 and NIC96.2 Publications

    Protein-protein interaction databases

    BioGridi35040. 223 interactions.
    DIPiDIP-2429N.
    IntActiP52593. 27 interactions.
    MINTiMINT-2780953.
    STRINGi4932.YML103C.

    Structurei

    3D structure databases

    ProteinModelPortaliP52593.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni250 – 27122Leucine-zipperAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili35 – 6228Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi485 – 4895Poly-Ser
    Compositional biasi533 – 54513Gln-richAdd
    BLAST
    Compositional biasi533 – 5419Poly-Gln
    Compositional biasi1385 – 13917Poly-Leu
    Compositional biasi1504 – 15074Poly-Leu

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG68960.
    HOGENOMiHOG000113877.
    KOiK14311.
    OMAiDFICAVM.
    OrthoDBiEOG7JDR69.

    Family and domain databases

    InterProiIPR018864. Nucleoporin_Nup188.
    [Graphical view]
    PfamiPF10487. Nup188. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52593-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATPSFGNSS PQLTFTHVAN FMNDAAADVS AVDAKQLAQI RQFLKANKTN     50
    LIESLNTIRQ NVTSSGDHNK LRSTIANLLQ INVDNDPFFA QSEDLSHAVE 100
    FFMSERSSRL HIVYSLLVNP DIDLETYSFI DNDRFNVVGK LISIISSVIQ 150
    NYDIITASSL AHDYNNDQDM FTIVSLVQLK KFSDLKFILQ ILQILNLMIL 200
    NTKVPVDIVN QWFLQYQNQF VEFCRNINST DKSIDTSSLQ LYKFQNFQDL 250
    SYLSETLISR ISSLFTITTI LILGLNTSIA QFDIQSPLYM DTETFDTVNS 300
    ALENDVATNI VNEDPIFHPM IHYSWSFILY YRRALQSSES FDDSDITKFA 350
    LFAESHDVLQ KLNTLSEILS FDPVYTTVIT VFLEFSLNFI PITASTSRVF 400
    AKIISKAPEQ FIENFLTNDT FEKKLSIIKA KLPLLNESLI PLINLALIDT 450
    EFANFELKDI CSFAVTKSSL NDLDYDLIAD TITNSSSSSD IIVPDLIELK 500
    SDLLVAPPLE NENSNCLLSI PKSTKGKILT IKQQQQQQQQ QNGQQPPTTS 550
    NLIIFLYKFN GWSLVGRILQ NLLHSYMEKG TQLDDLQHEL MISIIKLVTN 600
    VVDPKTSIEK SSEILSYLSN SLDTSASTIN GASIIQVIFE IFEISLQRKD 650
    YTSIVQCCEF MTMLTPNYLH LVSSYLNKSD LLDKYGKTGL SNMILGSVEL 700
    STGDYTFTIQ LLKLTKVFIR ESLSLKNIHI SKRSKIDIIN KLILHAIHIF 750
    ESYYNWKYNN FLQKFEIAFH LTLIFYDVLH DVFTINPHQK DQLIISSSAN 800
    KLLQLFLTPM DSIDLAPNTL TNILISPLNT TTKILGDKIL GNLYSKVMNN 850
    SFKLCTLLIA IRGSNRDLKP SNLEKLLFIN SSKLVDVYTL PSYVHFKVQI 900
    IELLSYLVEA PWNDDYPFLL SFLGEAKSMA FLKEVLSDLS SPVQDWNLLR 950
    SLYIFFTTLL ESKQDGLSIL FLTGQFASNK KINDESSIDK KSSILTVLQK 1000
    NSLLLDSTPE EVSCKLLETI TYVLNTWTNS KIFIKDPKFV NSLLAKLKDS 1050
    KKLFQKKENL TRDETVSLIK KYKLISRIVE IFALCIYNST DSNSEILNFL 1100
    NQEDLFELVH HFFQIDGFNK TFHDELNLKF KEKWPSLELQ SFQKIPLSRI 1150
    NENENFGYDI PLLDIVLKAD RSWNEPSKSQ TNFKEEITDA SLNLQYVNYE 1200
    ISTAKAWGAL ITTFVKRSTV PLNDGFVDLV EHFLKLNIDF GSDKQMFTQI 1250
    YLERIELSFY ILYSFKLSGK LLKEEKIIEL MNKIFTIFKS GEIDFIKNIG 1300
    KSLKNNFYRP LLRSVLVLLE LVSSGDRFIE LISDQLLEFF ELVFSKGVYL 1350
    ILSEILCQIN KCSTRGLSTD HTTQIVNLED NTQDLLLLLS LFKKITNVNP 1400
    SKNFNVILAS SLNEVGTLKV ILNLYSSAHL IRINDEPILG QITLTFISEL 1450
    CSIEPIAAKL INSGLYSVLL ESPLSVAIQQ GDIKPEFSPR LHNIWSNGLL 1500
    SIVLLLLSQF GIKVLPETCL FVSYFGKQIK STIYNWGDNK LAVSSSLIKE 1550
    TNQLVLLQKM LNLLNYQELF IQPKNSDDQQ EAVELVIGLD SEHDKKRLSA 1600
    ALSKFLTHPK YLNSRIIPTT LEEQQQLEDE SSRLEFVKGI SRDIKALQDS 1650
    LFKDV 1655
    Length:1,655
    Mass (Da):188,577
    Last modified:October 1, 1996 - v1
    Checksum:iB590051EEE60464C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90580 Genomic DNA. Translation: CAA62208.1.
    U47107 Genomic DNA. Translation: AAA88904.1.
    X80835 Genomic DNA. Translation: CAA56794.1.
    BK006946 Genomic DNA. Translation: DAA09795.1.
    PIRiS47446.
    RefSeqiNP_013604.1. NM_001182465.1.

    Genome annotation databases

    EnsemblFungiiYML103C; YML103C; YML103C.
    GeneIDi854868.
    KEGGisce:YML103C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90580 Genomic DNA. Translation: CAA62208.1 .
    U47107 Genomic DNA. Translation: AAA88904.1 .
    X80835 Genomic DNA. Translation: CAA56794.1 .
    BK006946 Genomic DNA. Translation: DAA09795.1 .
    PIRi S47446.
    RefSeqi NP_013604.1. NM_001182465.1.

    3D structure databases

    ProteinModelPortali P52593.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35040. 223 interactions.
    DIPi DIP-2429N.
    IntActi P52593. 27 interactions.
    MINTi MINT-2780953.
    STRINGi 4932.YML103C.

    Protein family/group databases

    TCDBi 1.I.1.1.1. the nuclear pore complex (npc) family.

    Proteomic databases

    MaxQBi P52593.
    PaxDbi P52593.
    PeptideAtlasi P52593.

    Protocols and materials databases

    DNASUi 854868.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML103C ; YML103C ; YML103C .
    GeneIDi 854868.
    KEGGi sce:YML103C.

    Organism-specific databases

    CYGDi YML103c.
    SGDi S000004571. NUP188.

    Phylogenomic databases

    eggNOGi NOG68960.
    HOGENOMi HOG000113877.
    KOi K14311.
    OMAi DFICAVM.
    OrthoDBi EOG7JDR69.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32687-MONOMER.

    Miscellaneous databases

    NextBioi 977798.

    Gene expression databases

    Genevestigatori P52593.

    Family and domain databases

    InterProi IPR018864. Nucleoporin_Nup188.
    [Graphical view ]
    Pfami PF10487. Nup188. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p."
      Zabel U., Doye V., Tekotte H., Wepf R., Grandi P., Hurt E.C.
      J. Cell Biol. 133:1141-1152(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH NIC96.
    2. "The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex."
      Nehrbass U., Rout M.P., Maguire S., Blobel G., Wozniak R.W.
      J. Cell Biol. 133:1153-1162(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH NIC96 AND POM152.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p."
      Aitchison J.D., Rout M.P., Marelli M., Blobel G., Wozniak R.W.
      J. Cell Biol. 131:1133-1148(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1187-1205 AND 1611-1629.
    6. "The yeast nuclear pore complex: composition, architecture, and transport mechanism."
      Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
      J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
    7. "Peering through the pore: nuclear pore complex structure, assembly, and function."
      Suntharalingam M., Wente S.R.
      Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-340, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNU188_YEAST
    AccessioniPrimary (citable) accession number: P52593
    Secondary accession number(s): D6W0I1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 11700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3