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P52593

- NU188_YEAST

UniProt

P52593 - NU188_YEAST

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Protein

Nucleoporin NUP188

Gene

NUP188

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP188 probably plays an important role in NPC assembly and organization.

GO - Molecular functioni

  1. structural constituent of nuclear pore Source: SGD

GO - Biological processi

  1. mRNA export from nucleus in response to heat stress Source: SGD
  2. nuclear pore organization Source: SGD
  3. protein import into nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32687-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP188
Alternative name(s):
Nuclear pore protein NUP188
Gene namesi
Name:NUP188
Ordered Locus Names:YML103C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML103c.
SGDiS000004571. NUP188.

Subcellular locationi

GO - Cellular componenti

  1. nuclear pore Source: SGD
  2. nuclear pore inner ring Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16551655Nucleoporin NUP188PRO_0000204856Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei340 – 3401Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP52593.
PaxDbiP52593.
PeptideAtlasiP52593.

Expressioni

Gene expression databases

GenevestigatoriP52593.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Interacts with POM152 and NIC96.2 Publications

Protein-protein interaction databases

BioGridi35040. 224 interactions.
DIPiDIP-2429N.
IntActiP52593. 27 interactions.
MINTiMINT-2780953.
STRINGi4932.YML103C.

Structurei

3D structure databases

ProteinModelPortaliP52593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni250 – 27122Leucine-zipperAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili35 – 6228Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi485 – 4895Poly-Ser
Compositional biasi533 – 54513Gln-richAdd
BLAST
Compositional biasi533 – 5419Poly-Gln
Compositional biasi1385 – 13917Poly-Leu
Compositional biasi1504 – 15074Poly-Leu

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG68960.
HOGENOMiHOG000113877.
InParanoidiP52593.
KOiK14311.
OMAiDFICAVM.
OrthoDBiEOG7JDR69.

Family and domain databases

InterProiIPR018864. Nucleoporin_Nup188.
[Graphical view]
PfamiPF10487. Nup188. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52593-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATPSFGNSS PQLTFTHVAN FMNDAAADVS AVDAKQLAQI RQFLKANKTN
60 70 80 90 100
LIESLNTIRQ NVTSSGDHNK LRSTIANLLQ INVDNDPFFA QSEDLSHAVE
110 120 130 140 150
FFMSERSSRL HIVYSLLVNP DIDLETYSFI DNDRFNVVGK LISIISSVIQ
160 170 180 190 200
NYDIITASSL AHDYNNDQDM FTIVSLVQLK KFSDLKFILQ ILQILNLMIL
210 220 230 240 250
NTKVPVDIVN QWFLQYQNQF VEFCRNINST DKSIDTSSLQ LYKFQNFQDL
260 270 280 290 300
SYLSETLISR ISSLFTITTI LILGLNTSIA QFDIQSPLYM DTETFDTVNS
310 320 330 340 350
ALENDVATNI VNEDPIFHPM IHYSWSFILY YRRALQSSES FDDSDITKFA
360 370 380 390 400
LFAESHDVLQ KLNTLSEILS FDPVYTTVIT VFLEFSLNFI PITASTSRVF
410 420 430 440 450
AKIISKAPEQ FIENFLTNDT FEKKLSIIKA KLPLLNESLI PLINLALIDT
460 470 480 490 500
EFANFELKDI CSFAVTKSSL NDLDYDLIAD TITNSSSSSD IIVPDLIELK
510 520 530 540 550
SDLLVAPPLE NENSNCLLSI PKSTKGKILT IKQQQQQQQQ QNGQQPPTTS
560 570 580 590 600
NLIIFLYKFN GWSLVGRILQ NLLHSYMEKG TQLDDLQHEL MISIIKLVTN
610 620 630 640 650
VVDPKTSIEK SSEILSYLSN SLDTSASTIN GASIIQVIFE IFEISLQRKD
660 670 680 690 700
YTSIVQCCEF MTMLTPNYLH LVSSYLNKSD LLDKYGKTGL SNMILGSVEL
710 720 730 740 750
STGDYTFTIQ LLKLTKVFIR ESLSLKNIHI SKRSKIDIIN KLILHAIHIF
760 770 780 790 800
ESYYNWKYNN FLQKFEIAFH LTLIFYDVLH DVFTINPHQK DQLIISSSAN
810 820 830 840 850
KLLQLFLTPM DSIDLAPNTL TNILISPLNT TTKILGDKIL GNLYSKVMNN
860 870 880 890 900
SFKLCTLLIA IRGSNRDLKP SNLEKLLFIN SSKLVDVYTL PSYVHFKVQI
910 920 930 940 950
IELLSYLVEA PWNDDYPFLL SFLGEAKSMA FLKEVLSDLS SPVQDWNLLR
960 970 980 990 1000
SLYIFFTTLL ESKQDGLSIL FLTGQFASNK KINDESSIDK KSSILTVLQK
1010 1020 1030 1040 1050
NSLLLDSTPE EVSCKLLETI TYVLNTWTNS KIFIKDPKFV NSLLAKLKDS
1060 1070 1080 1090 1100
KKLFQKKENL TRDETVSLIK KYKLISRIVE IFALCIYNST DSNSEILNFL
1110 1120 1130 1140 1150
NQEDLFELVH HFFQIDGFNK TFHDELNLKF KEKWPSLELQ SFQKIPLSRI
1160 1170 1180 1190 1200
NENENFGYDI PLLDIVLKAD RSWNEPSKSQ TNFKEEITDA SLNLQYVNYE
1210 1220 1230 1240 1250
ISTAKAWGAL ITTFVKRSTV PLNDGFVDLV EHFLKLNIDF GSDKQMFTQI
1260 1270 1280 1290 1300
YLERIELSFY ILYSFKLSGK LLKEEKIIEL MNKIFTIFKS GEIDFIKNIG
1310 1320 1330 1340 1350
KSLKNNFYRP LLRSVLVLLE LVSSGDRFIE LISDQLLEFF ELVFSKGVYL
1360 1370 1380 1390 1400
ILSEILCQIN KCSTRGLSTD HTTQIVNLED NTQDLLLLLS LFKKITNVNP
1410 1420 1430 1440 1450
SKNFNVILAS SLNEVGTLKV ILNLYSSAHL IRINDEPILG QITLTFISEL
1460 1470 1480 1490 1500
CSIEPIAAKL INSGLYSVLL ESPLSVAIQQ GDIKPEFSPR LHNIWSNGLL
1510 1520 1530 1540 1550
SIVLLLLSQF GIKVLPETCL FVSYFGKQIK STIYNWGDNK LAVSSSLIKE
1560 1570 1580 1590 1600
TNQLVLLQKM LNLLNYQELF IQPKNSDDQQ EAVELVIGLD SEHDKKRLSA
1610 1620 1630 1640 1650
ALSKFLTHPK YLNSRIIPTT LEEQQQLEDE SSRLEFVKGI SRDIKALQDS

LFKDV
Length:1,655
Mass (Da):188,577
Last modified:October 1, 1996 - v1
Checksum:iB590051EEE60464C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X90580 Genomic DNA. Translation: CAA62208.1.
U47107 Genomic DNA. Translation: AAA88904.1.
X80835 Genomic DNA. Translation: CAA56794.1.
BK006946 Genomic DNA. Translation: DAA09795.1.
PIRiS47446.
RefSeqiNP_013604.1. NM_001182465.1.

Genome annotation databases

EnsemblFungiiYML103C; YML103C; YML103C.
GeneIDi854868.
KEGGisce:YML103C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X90580 Genomic DNA. Translation: CAA62208.1 .
U47107 Genomic DNA. Translation: AAA88904.1 .
X80835 Genomic DNA. Translation: CAA56794.1 .
BK006946 Genomic DNA. Translation: DAA09795.1 .
PIRi S47446.
RefSeqi NP_013604.1. NM_001182465.1.

3D structure databases

ProteinModelPortali P52593.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35040. 224 interactions.
DIPi DIP-2429N.
IntActi P52593. 27 interactions.
MINTi MINT-2780953.
STRINGi 4932.YML103C.

Protein family/group databases

TCDBi 1.I.1.1.1. the nuclear pore complex (npc) family.

Proteomic databases

MaxQBi P52593.
PaxDbi P52593.
PeptideAtlasi P52593.

Protocols and materials databases

DNASUi 854868.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML103C ; YML103C ; YML103C .
GeneIDi 854868.
KEGGi sce:YML103C.

Organism-specific databases

CYGDi YML103c.
SGDi S000004571. NUP188.

Phylogenomic databases

eggNOGi NOG68960.
HOGENOMi HOG000113877.
InParanoidi P52593.
KOi K14311.
OMAi DFICAVM.
OrthoDBi EOG7JDR69.

Enzyme and pathway databases

BioCyci YEAST:G3O-32687-MONOMER.

Miscellaneous databases

NextBioi 977798.

Gene expression databases

Genevestigatori P52593.

Family and domain databases

InterProi IPR018864. Nucleoporin_Nup188.
[Graphical view ]
Pfami PF10487. Nup188. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p."
    Zabel U., Doye V., Tekotte H., Wepf R., Grandi P., Hurt E.C.
    J. Cell Biol. 133:1141-1152(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH NIC96.
  2. "The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex."
    Nehrbass U., Rout M.P., Maguire S., Blobel G., Wozniak R.W.
    J. Cell Biol. 133:1153-1162(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH NIC96 AND POM152.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p."
    Aitchison J.D., Rout M.P., Marelli M., Blobel G., Wozniak R.W.
    J. Cell Biol. 131:1133-1148(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1187-1205 AND 1611-1629.
  6. "The yeast nuclear pore complex: composition, architecture, and transport mechanism."
    Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
    J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
  7. "Peering through the pore: nuclear pore complex structure, assembly, and function."
    Suntharalingam M., Wente S.R.
    Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-340, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNU188_YEAST
AccessioniPrimary (citable) accession number: P52593
Secondary accession number(s): D6W0I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3