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Protein

Sphingosine 1-phosphate receptor 2

Gene

S1pr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues.

GO - Molecular functioni

  1. G-protein coupled receptor binding Source: MGI
  2. integrin binding Source: MGI
  3. sphingosine-1-phosphate receptor activity Source: InterPro

GO - Biological processi

  1. negative regulation of excitatory postsynaptic membrane potential Source: MGI
  2. positive regulation of establishment of endothelial barrier Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_232027. Lysosphingolipid and LPA receptors.
REACT_250376. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine 1-phosphate receptor 2
Short name:
S1P receptor 2
Short name:
S1P2
Alternative name(s):
Endothelial differentiation G-protein coupled receptor 5
Lysophospholipid receptor B2
Sphingosine 1-phosphate receptor Edg-5
Short name:
S1P receptor Edg-5
Gene namesi
Name:S1pr2
Synonyms:Edg5, Gpcr13, Lpb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:99569. S1pr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3434ExtracellularBy similarityAdd
BLAST
Transmembranei35 – 5925Helical; Name=1By similarityAdd
BLAST
Topological domaini60 – 667CytoplasmicBy similarity
Transmembranei67 – 9529Helical; Name=2By similarityAdd
BLAST
Topological domaini96 – 10914ExtracellularBy similarityAdd
BLAST
Transmembranei110 – 12819Helical; Name=3By similarityAdd
BLAST
Topological domaini129 – 14719CytoplasmicBy similarityAdd
BLAST
Transmembranei148 – 17326Helical; Name=4By similarityAdd
BLAST
Topological domaini174 – 18916ExtracellularBy similarityAdd
BLAST
Transmembranei190 – 21021Helical; Name=5By similarityAdd
BLAST
Topological domaini211 – 23323CytoplasmicBy similarityAdd
BLAST
Transmembranei234 – 25522Helical; Name=6By similarityAdd
BLAST
Topological domaini256 – 27116ExtracellularBy similarityAdd
BLAST
Transmembranei272 – 29221Helical; Name=7By similarityAdd
BLAST
Topological domaini293 – 35260CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Sphingosine 1-phosphate receptor 2PRO_0000069428Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi19 – 191N-linked (GlcNAc...)2 Publications
Lipidationi305 – 3051S-palmitoyl cysteineBy similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP52592.
PRIDEiP52592.

PTM databases

PhosphoSiteiP52592.

Expressioni

Tissue specificityi

Most abundant in heart and lung; low, but clearly observed in kidney, liver and thymus; much lower but detectable in brain, testis, stomach and intestine. Not significantly detected in any of the sections of embryonic day (E) 14-18, except in embryonic brain.1 Publication

Gene expression databases

BgeeiP52592.
GenevestigatoriP52592.

Interactioni

Protein-protein interaction databases

DIPiDIP-60681N.
STRINGi10090.ENSMUSP00000053394.

Structurei

3D structure databases

ProteinModelPortaliP52592.
SMRiP52592. Positions 6-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG149362.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiP52592.
KOiK04292.
OMAiKFHSAMY.
OrthoDBiEOG708W0B.
TreeFamiTF330052.

Family and domain databases

InterProiIPR004063. EDG5_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PANTHERiPTHR22750:SF17. PTHR22750:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01525. EDG5RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52592-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGGLYSEYLN PEKVLEHYNY TKETLDMQET TSRKVASAFI IILCCAIVVE
60 70 80 90 100
NLLVLIAVAR NSKFHSAMYL FLGNLAASDL LAGVAFVANT LLSGHVTLSL
110 120 130 140 150
TPVQWFAREG SAFITLSASV FSLLAIAIER QVALAKVKLY GSDKSCRMLM
160 170 180 190 200
LIGASWLISL ILGGLPILGW NCLNQLEACS TVLPLYAKHY VLCVVTIFSV
210 220 230 240 250
ILLAIVALYV RIYFVVRSSH ADVAGPQTLA LLKTVTIVLG VFIICWLPAF
260 270 280 290 300
SILLLDSTCP VRACPVLYKA HYFFAFATLN SLLNPVIYTW RSRDLRREVL
310 320 330 340 350
RPLQCWRRGK GVTGRRGGNP GHRLLPLRSS SSLERGMHMP TSPTFLEGNT

VV
Length:352
Mass (Da):38,829
Last modified:July 27, 2011 - v3
Checksum:i6A3E426B0FE54406
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101G → V in AAD16976. (PubMed:9931453)Curated
Sequence conflicti110 – 1101G → V in AAA16846. (PubMed:8288218)Curated
Sequence conflicti166 – 1661P → S in AAA16846. (PubMed:8288218)Curated
Sequence conflicti175 – 1751Q → K in AAA16846. (PubMed:8288218)Curated
Sequence conflicti189 – 1891H → R in AAA16846. (PubMed:8288218)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF108020 Genomic DNA. Translation: AAD16976.1.
AK085114 mRNA. Translation: BAC39368.1.
AK134275 mRNA. Translation: BAE22078.1.
AK151062 mRNA. Translation: BAE30079.1.
AK159605 mRNA. Translation: BAE35224.1.
AK170436 mRNA. Translation: BAE41795.1.
CH466522 Genomic DNA. Translation: EDL25146.1.
BC096760 mRNA. Translation: AAH96760.1.
L20334 mRNA. Translation: AAA16846.1.
CCDSiCCDS22888.1.
PIRiE48909.
RefSeqiNP_034463.2. NM_010333.4.
XP_006510082.1. XM_006510019.1.
UniGeneiMm.46493.

Genome annotation databases

EnsembliENSMUST00000054197; ENSMUSP00000053394; ENSMUSG00000043895.
GeneIDi14739.
KEGGimmu:14739.
UCSCiuc009ojt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF108020 Genomic DNA. Translation: AAD16976.1.
AK085114 mRNA. Translation: BAC39368.1.
AK134275 mRNA. Translation: BAE22078.1.
AK151062 mRNA. Translation: BAE30079.1.
AK159605 mRNA. Translation: BAE35224.1.
AK170436 mRNA. Translation: BAE41795.1.
CH466522 Genomic DNA. Translation: EDL25146.1.
BC096760 mRNA. Translation: AAH96760.1.
L20334 mRNA. Translation: AAA16846.1.
CCDSiCCDS22888.1.
PIRiE48909.
RefSeqiNP_034463.2. NM_010333.4.
XP_006510082.1. XM_006510019.1.
UniGeneiMm.46493.

3D structure databases

ProteinModelPortaliP52592.
SMRiP52592. Positions 6-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60681N.
STRINGi10090.ENSMUSP00000053394.

Chemistry

GuidetoPHARMACOLOGYi276.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP52592.

Proteomic databases

PaxDbiP52592.
PRIDEiP52592.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054197; ENSMUSP00000053394; ENSMUSG00000043895.
GeneIDi14739.
KEGGimmu:14739.
UCSCiuc009ojt.2. mouse.

Organism-specific databases

CTDi9294.
MGIiMGI:99569. S1pr2.

Phylogenomic databases

eggNOGiNOG149362.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiP52592.
KOiK04292.
OMAiKFHSAMY.
OrthoDBiEOG708W0B.
TreeFamiTF330052.

Enzyme and pathway databases

ReactomeiREACT_232027. Lysosphingolipid and LPA receptors.
REACT_250376. G alpha (i) signalling events.

Miscellaneous databases

ChiTaRSiS1pr2. mouse.
NextBioi286795.
PROiP52592.
SOURCEiSearch...

Gene expression databases

BgeeiP52592.
GenevestigatoriP52592.

Family and domain databases

InterProiIPR004063. EDG5_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PANTHERiPTHR22750:SF17. PTHR22750:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01525. EDG5RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative analysis of three murine G-protein coupled receptors activated by sphingosine-1-phosphate."
    Zhang G., Contos J.J.A., Weiner J.A., Fukushima N., Chun J.
    Gene 227:89-99(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: 129/SvJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Forelimb and Lung.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Identification, chromosomal location, and genome organization of mammalian G-protein-coupled receptors."
    Wilkie T.M., Chen Y., Gilbert D.J., Moore K.J., Yu L., Simon M.I., Copeland N.G., Jenkins N.A.
    Genomics 18:175-184(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 62-241.
    Tissue: Testis.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-19.
    Tissue: Myoblast.
  8. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-19.

Entry informationi

Entry nameiS1PR2_MOUSE
AccessioniPrimary (citable) accession number: P52592
Secondary accession number(s): Q8C3Q7, Q9R236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: February 4, 2015
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.