P52589 (PDI_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein disulfide-isomerase
Short name=PDI
EC=5.3.4.1

Gene names

Name:

PDI

Organism

Triticum aestivum (Wheat)

Taxonomic identifier

4565 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer By similarity.
Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen Potential.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glycerol ether metabolic process Inferred from electronic annotation. Source: InterPro protein folding Inferred from electronic annotation. Source: GOC
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro protein disulfide isomerase activity Inferred from electronic annotation. Source: EC protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	By similarity
	Chain	26 – 515	490	Protein disulfide-isomerase		PRO_0000034212
Regions
	Domain	26 – 150	125	Thioredoxin 1
	Domain	346 – 489	144	Thioredoxin 2
	Motif	512 – 515	4	Prevents secretion from ER Potential
Sites
	Active site	68	1	Nucleophile By similarity
	Active site	71	1	Nucleophile By similarity
	Active site	412	1	Nucleophile By similarity
	Active site	415	1	Nucleophile By similarity
	Site	69	1	Contributes to redox potential value By similarity
	Site	70	1	Contributes to redox potential value By similarity
	Site	136	1	Lowers pKa of C-terminal Cys of first active site By similarity
	Site	413	1	Contributes to redox potential value By similarity
	Site	414	1	Contributes to redox potential value By similarity
	Site	475	1	Lowers pKa of C-terminal Cys of second active site By similarity
Amino acid modifications
	Glycosylation	283	1	N-linked (GlcNAc...) Potential
	Disulfide bond	68 ↔ 71		Redox-active By similarity
	Disulfide bond	412 ↔ 415		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52589 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6630AB9A252A96B6	FASTA	515	56,533
	10 20 30 40 50 60 MAISKVWISL LLALAVVLSA PAARAEEAAA AEEAAAAPEA VLTLHADNFD DAIAKHPFIL 70 80 90 100 110 120 VEFYAPWCGH CKSLAPEYEK AAQLLSKHDP AIVLAKVDAN DEKNKPLAGK YEVQGFPTLK 130 140 150 160 170 180 IFRSGGKNIQ EYKGPREAEG IVEYLKKQVG PASKEIKAPE DATYLEDGKI HIVGVFTEFS 190 200 210 220 230 240 GTEFTNFLEL AEKLRSDYDF GHTVHANHLP RGDAAVERPL VRLFKPFDEL VVDSKDFDVS 250 260 270 280 290 300 ALEKFIDASS TPKVVTFDKN PDNHPYLLKY FQSNAPKAML FLNFSTGPFE SFKSAYYGAV 310 320 330 340 350 360 EEFSGKDVKF LIGDIEASQG AFQYNGLKED QAPLILIQDS DSKKFLKEQV EAGQIVAWLK 370 380 390 400 410 420 DYFDGKLTPF RKSEPIPEAN NEPVKVVVAD NIHDVVFKSA KNVLIEFYAP WCGHCKKLAP 430 440 450 460 470 480 ILDEAAATLQ SEEDVVIAKI DATANDVPGE FDVQGYPTLY FVTPSGKKVS YEGGRTADEI 490 500 510 VDYIKKNKET AGQAAAAATE KAAEPAATEP LKDEL

References

[1]	"Nucleotide sequence of a wheat cDNA encoding protein disulfide isomerase." Shimoni Y., Segal G., Zhu X.Z., Galili G. Plant Physiol. 107:281-281(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Chinese Spring.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11496 mRNA. Translation: AAA19660.1.
PIR	T06262.
UniGene	Ta.2019.
3D structure databases
ProteinModelPortal	P52589.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P52589.
Family and domain databases
Gene3D	3.40.30.10. 3 hits.
InterPro	IPR005788. Disulphide_isomerase. IPR005792. Prot_disulphide_isomerase. IPR005746. Thioredoxin. IPR012336. Thioredoxin-like_fold. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
SUPFAM	SSF52833. Thiordxn-like_fd. 4 hits.
TIGRFAMs	TIGR01130. ER_PDI_fam. 1 hit. TIGR01126. pdi_dom. 2 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDI_WHEAT

Accession

Primary (citable) accession number: P52589

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program