ID   IFR_PEA                 Reviewed;         318 AA.
AC   P52576;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 45.
DE   RecName: Full=Isoflavone reductase;
DE            Short=IFR;
DE            EC=1.3.1.45;
DE   AltName: Full=2'-hydroxyisoflavone reductase;
DE   AltName: Full=NADPH:isoflavone oxidoreductase;
GN   Name=IFR;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Alaska Green;
RX   MEDLINE=94311603; PubMed=8037464; DOI=10.1006/abbi.1994.1338;
RA   Paiva N.L., Sun Y., Dixon R.A., Vanetten H.D., Hrazdina G.;
RT   "Molecular cloning of isoflavone reductase from pea (Pisum sativum
RT   L.): evidence for a 3R-isoflavanone intermediate in (+)-pisatin
RT   biosynthesis.";
RL   Arch. Biochem. Biophys. 312:501-510(1994).
CC   -!- FUNCTION: Reduces achiral isoflavones to chiral isoflavanones
CC       during the biosynthesis of chiral pterocarpan phytoalexins. The
CC       reduction product (sophrol) is a third isomer, which represents
CC       the penultimate intermediate in the synthesis of the phytoalexin
CC       (+)-pisatin, the major phytoalexin in pea.
CC   -!- CATALYTIC ACTIVITY: Vestitone + NADP(+) = 2'-hydroxyformononetin +
CC       NADPH.
CC   -!- PATHWAY: Phytoalexin biosynthesis; pterocarpan phytoalexin
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the NmrA-type oxidoreductase family.
CC       Isoflavone reductase subfamily.
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DR   EMBL; S72472; AAB31368.1; -; mRNA.
DR   PIR; S48631; S48631.
DR   SMR; P52576; 3-318.
DR   BRENDA; 1.3.1.45; 287.
DR   GO; GO:0047526; F:2'-hydroxyisoflavone reductase activity; IEA:EC.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0016564; F:transcription repressor activity; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006808; P:regulation of nitrogen utilization; IEA:InterPro.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR008030; NmrA.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF05368; NmrA; 1.
PE   2: Evidence at transcript level;
KW   NADP; Oxidoreductase.
FT   CHAIN         1    318       Isoflavone reductase.
FT                                /FTId=PRO_0000204547.
FT   NP_BIND       6     37       NADP (Potential).
SQ   SEQUENCE   318 AA;  35429 MW;  6318CF6617CDE28F CRC64;
     MATENKILIL GATGAIGRHI VWASIKAGNP TYALVRKTSD NVNKPKLTEA ANPETKEELL
     KNYQASGVIL LEGDINDHET LVNAIKQVDT VICAAGRLLI EDQVKVIKAI KEAGNVKRFF
     PSEFGLDVDR HDAVEPVRQV FEEKASIRRV VESEGVPYTY LCCHAFTGYF LRNLAQIDAT
     DPPRDKVVIL GDGNVRGAYV TEADVGTYTI RAANDPNTLN KAVHIRLPNN YLTANEVIAL
     WEKKIGKTLE KTYVSEEQVL KDIQTSSFPH NYLLALYHSQ QIKGDAVYEI DPAKDVEAYD
     AYPDVKYTTA DEYLNQFV
//