Reviewed, UniProtKB/Swiss-Prot P52576 (IFR_PEA)

Names and origin

Protein names

Recommended name:
    Isoflavone reductase
      Short name=IFR
    EC=1.3.1.45
Alternative name(s):
    2'-hydroxyisoflavone reductase
    NADPH:isoflavone oxidoreductase

Gene names

Name:

IFR

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Reduces achiral isoflavones to chiral isoflavanones during the biosynthesis of chiral pterocarpan phytoalexins. The reduction product (sophrol) is a third isomer, which represents the penultimate intermediate in the synthesis of the phytoalexin (+)-pisatin, the major phytoalexin in pea.
Catalytic activity	Vestitone + NADP+ = 2'-hydroxyformononetin + NADPH.
Pathway	Phytoalexin biosynthesis; pterocarpan phytoalexin biosynthesis.
Sequence similarities	Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of nitrogen utilization Inferred from electronic annotation. Source: InterPro
Molecular function	2'-hydroxyisoflavone reductase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro transcription repressor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Isoflavone reductase		PRO_0000204547
Regions
	Nucleotide binding	6 – 37	32	NADP Potential

Sequences

	Sequence		Length	Mass (Da)
	P52576-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6318CF6617CDE28F	FASTA	318	35,429
	10 20 30 40 50 60 MATENKILIL GATGAIGRHI VWASIKAGNP TYALVRKTSD NVNKPKLTEA ANPETKEELL 70 80 90 100 110 120 KNYQASGVIL LEGDINDHET LVNAIKQVDT VICAAGRLLI EDQVKVIKAI KEAGNVKRFF 130 140 150 160 170 180 PSEFGLDVDR HDAVEPVRQV FEEKASIRRV VESEGVPYTY LCCHAFTGYF LRNLAQIDAT 190 200 210 220 230 240 DPPRDKVVIL GDGNVRGAYV TEADVGTYTI RAANDPNTLN KAVHIRLPNN YLTANEVIAL 250 260 270 280 290 300 WEKKIGKTLE KTYVSEEQVL KDIQTSSFPH NYLLALYHSQ QIKGDAVYEI DPAKDVEAYD 310 AYPDVKYTTA DEYLNQFV

References

[1]

"Molecular cloning of isoflavone reductase from pea (Pisum sativum L.): evidence for a 3R-isoflavanone intermediate in (+)-pisatin biosynthesis." Paiva N.L., Sun Y., Dixon R.A., Vanetten H.D., Hrazdina G. Arch. Biochem. Biophys. 312:501-510(1994) [PubMed: 8037464] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Alaska Green.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S72472 mRNA. Translation: AAB31368.1.
PIR	S48631.
3D structure databases
SMR	P52576. Positions 3-318.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.1.45. 287.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR008030. NmrA. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF05368. NmrA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IFR_PEA

Accession

Primary (citable) accession number: P52576

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)