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P52575 (IFR_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoflavone reductase

Short name=IFR
EC=1.3.1.45
Alternative name(s):
2'-hydroxyisoflavone reductase
NADPH:isoflavone oxidoreductase
Gene names
Name:IFR
OrganismMedicago sativa (Alfalfa)
Taxonomic identifier3879 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reduces achiral isoflavones to chiral isoflavanones during the biosynthesis of chiral pterocarpan phytoalexins. The reduction product is a third isomer, which represents the penultimate intermediate in the synthesis of the phytoalexin (-)-medicarpin, the major phytoalexin in Alfalfa.

Catalytic activity

Vestitone + NADP+ = 2'-hydroxyformononetin + NADPH.

Pathway

Phytoalexin biosynthesis; pterocarpan phytoalexin biosynthesis.

Induction

By fungal elicitor.

Sequence similarities

Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.

Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
   Technical term3D-structure
Gene Ontology (GO)
   Molecular function2'-hydroxyisoflavone reductase activity

Inferred from electronic annotation. Source: EC

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Isoflavone reductase
PRO_0000204546

Regions

Nucleotide binding6 – 3732NADP Potential

Experimental info

Sequence conflict1321E → D in CAA41106. Ref.2
Sequence conflict1791T → A in CAA41106. Ref.2
Sequence conflict2291E → K in CAA41106. Ref.2

Secondary structure

........................................................ 318
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P52575 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 5E2AF9C8CFB79F17

FASTA31835,455
        10         20         30         40         50         60 
MATENKILIL GPTGAIGRHI VWASIKAGNP TYALVRKTPG NVNKPKLITA ANPETKEELI 

        70         80         90        100        110        120 
DNYQSLGVIL LEGDINDHET LVKAIKQVDI VICAAGRLLI EDQVKIIKAI KEAGNVKKFF 

       130        140        150        160        170        180 
PSEFGLDVDR HEAVEPVRQV FEEKASIRRV IEAEGVPYTY LCCHAFTGYF LRNLAQLDTT 

       190        200        210        220        230        240 
DPPRDKVVIL GDGNVKGAYV TEADVGTFTI RAANDPNTLN KAVHIRLPEN YLTQNEVIAL 

       250        260        270        280        290        300 
WEKKIGKTLE KTYVSEEQVL KDIQESSFPH NYLLALYHSQ QIKGDAVYEI DPAKDIEASE 

       310 
AYPDVTYTTA DEYLNQFV 

« Hide

References

[1]"The elicitor-inducible alfalfa isoflavone reductase promoter confers different patterns of developmental expression in homologous and heterologous transgenic plants."
Oommen A., Dixon R.A., Paiva N.L.
Plant Cell 6:1789-1803(1994) [PubMed: 7866024] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Stress responses in alfalfa (Medicago sativa L.) 11. Molecular cloning and expression of alfalfa isoflavone reductase, a key enzyme of isoflavonoid phytoalexin biosynthesis."
Paiva N.L., Edwards R., Sun Y., Hrazdina G., Dixon R.A.
Plant Mol. Biol. 17:653-667(1991) [PubMed: 1912490] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Apollo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U17436 Genomic DNA. Translation: AAC48976.1.
X58078 mRNA. Translation: CAA41106.1.
PIRS17744.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2GASX-ray1.60A/B3-318[»]
ProteinModelPortalP52575.
SMRP52575. Positions 3-318.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR016040. NAD(P)-bd_dom.
IPR008030. NmrA.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF05368. NmrA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIFR_MEDSA
AccessionPrimary (citable) accession number: P52575
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families