P52569 (CTR2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Low affinity cationic amino acid transporter 2
Short name=CAT-2
Short name=CAT2

Alternative name(s):
Solute carrier family 7 member 2

Gene names

Name:	SLC7A2
Synonyms:	ATRC2, CAT2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Low-affinity, high capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine). Plays a regulatory role in classical or alternative activation of macrophages By similarity. Ref.2
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed at high levels in the skeletal muscle, placenta and ovary. Expressed at intermediate levels in the liver and pancreas and at low levels in the kidney and heart. Ref.1
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family. [View classification]
Sequence caution	The sequence AAH69648.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAI04906.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAI13662.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAI43584.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. Isoform 2: The sequence AAB62810.1 differs from that shown. Reason: Frameshift at position 29.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular amino acid metabolic process Traceable author statement Ref.1. Source: ProtInc macrophage activation Inferred from electronic annotation. Source: Compara nitric oxide biosynthetic process Inferred from electronic annotation. Source: Compara nitric oxide production involved in inflammatory response Inferred from electronic annotation. Source: Compara regulation of inflammatory response Inferred from electronic annotation. Source: Compara regulation of macrophage activation Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	L-lysine transmembrane transporter activity Inferred from electronic annotation. Source: Compara L-ornithine transmembrane transporter activity Inferred from electronic annotation. Source: Compara basic amino acid transmembrane transporter activity Traceable author statement Ref.1. Source: ProtInc high affinity arginine transmembrane transporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P52569-1)

Also known as: CAT-2B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P52569-2)

Also known as: CAT-2A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKIETSGYNSDKLICRGFIGTPAPPVCDSKFLLSPSSDVRM
     357-398: IFPMPRVIYA...IATLSSGAVA → MFPLPRILFA...AATLTAGVIS

Isoform 3 (identifier: P52569-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKIETSGYNSDKLICRGFIGTPAPPVCDSKFLLSPSSDVRM

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 658	658	Low affinity cationic amino acid transporter 2		PRO_0000054264
Regions
	Topological domain	1 – 37	37	Cytoplasmic Potential
	Transmembrane	38 – 59	22	Helical; Potential
	Topological domain	60 – 63	4	Extracellular Potential
	Transmembrane	64 – 84	21	Helical; Potential
	Topological domain	85 – 104	20	Cytoplasmic Potential
	Transmembrane	105 – 125	21	Helical; Potential
	Topological domain	126 – 163	38	Extracellular Potential
	Transmembrane	164 – 184	21	Helical; Potential
	Topological domain	185 – 192	8	Cytoplasmic Potential
	Transmembrane	193 – 213	21	Helical; Potential
	Topological domain	214 – 248	35	Extracellular Potential
	Transmembrane	249 – 269	21	Helical; Potential
	Topological domain	270 – 289	20	Cytoplasmic Potential
	Transmembrane	290 – 309	20	Helical; Potential
	Topological domain	310 – 339	30	Extracellular Potential
	Transmembrane	340 – 360	21	Helical; Potential
	Topological domain	361 – 386	26	Cytoplasmic Potential
	Transmembrane	387 – 407	21	Helical; Potential
	Topological domain	408 – 410	3	Extracellular Potential
	Transmembrane	411 – 431	21	Helical; Potential
	Topological domain	432 – 490	59	Cytoplasmic Potential
	Transmembrane	491 – 511	21	Helical; Potential
	Topological domain	512 – 524	13	Extracellular Potential
	Transmembrane	525 – 549	25	Helical; Potential
	Topological domain	550 – 557	8	Cytoplasmic Potential
	Transmembrane	558 – 578	21	Helical; Potential
	Topological domain	579 – 582	4	Extracellular Potential
	Transmembrane	583 – 603	21	Helical; Potential
	Topological domain	604 – 658	55	Cytoplasmic Potential
Amino acid modifications
	Modified residue	455	1	Phosphoserine Ref.8
	Modified residue	646	1	Phosphoserine Ref.7
	Glycosylation	157	1	N-linked (GlcNAc...) Potential
	Glycosylation	227	1	N-linked (GlcNAc...) Potential
	Glycosylation	239	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1	1	M → MKIETSGYNSDKLICRGFIG TPAPPVCDSKFLLSPSSDVR M in isoform 2 and isoform 3.		VSP_037354
	Alternative sequence	357 – 398	42	IFPMP…SGAVA → MFPLPRILFAMARDGLLFRF LARVSKRQSPVAATLTAGVI S in isoform 2.		VSP_023354
	Natural variant	20	1	V → M. Corresponds to variant rs12680645 [ dbSNP | Ensembl ].		VAR_030799
	Natural variant	376	1	C → F. Corresponds to variant rs1134975 [ dbSNP | Ensembl ].		VAR_030800
	Natural variant	531	1	A → T. Ref.2 Ref.5 Corresponds to variant rs62622371 [ dbSNP | Ensembl ].		VAR_030801
	Natural variant	547	1	Q → L. Corresponds to variant rs1981498 [ dbSNP | Ensembl ].		VAR_058414
Experimental info
	Sequence conflict	134	1	W → G in BAA06271. Ref.1
	Sequence conflict	364	1	I → N in BAA06271. Ref.1
	Sequence conflict	520	1	T → Y in BAA06271. Ref.1
	Sequence conflict	552	1	Q → R in BAA06271. Ref.1
	Sequence conflict	566	1	A → T in BAA06271. Ref.1
	Sequence conflict	568	1	S → T in BAA06271. Ref.1
	Sequence conflict	605	1	H → R in CAD89909. Ref.6
	Sequence conflict	619	1	D → V in CAD89909. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CAT-2B) [UniParc]. Last modified February 20, 2007. Version 2. Checksum: 633FD86A333E9208	FASTA	658	71,673
	10 20 30 40 50 60 MIPCRAALTF ARCLIRRKIV TLDSLEDTKL CRCLSTMDLI ALGVGSTLGA GVYVLAGEVA 70 80 90 100 110 120 KADSGPSIVV SFLIAALASV MAGLCYAEFG ARVPKTGSAY LYTYVTVGEL WAFITGWNLI 130 140 150 160 170 180 LSYVIGTSSV ARAWSGTFDE LLSKQIGQFL RTYFRMNYTG LAEYPDFFAV CLILLLAGLL 190 200 210 220 230 240 SFGVKESAWV NKVFTAVNIL VLLFVMVAGF VKGNVANWKI SEEFLKNISA SAREPPSENG 250 260 270 280 290 300 TSIYGAGGFM PYGFTGTLAG AATCFYAFVG FDCIATTGEE VRNPQKAIPI GIVTSLLVCF 310 320 330 340 350 360 MAYFGVSAAL TLMMPYYLLD EKSPLPVAFE YVGWGPAKYV VAAGSLCALS TSLLGSIFPM 370 380 390 400 410 420 PRVIYAMAED GLLFKCLAQI NSKTKTPIIA TLSSGAVAAL MAFLFDLKAL VDMMSIGTLM 430 440 450 460 470 480 AYSLVAACVL ILRYQPGLSY DQPKCSPEKD GLGSSPRVTS KSESQVTMLQ RQGFSMRTLF 490 500 510 520 530 540 CPSLLPTQQS ASLVSFLVGF LAFLVLGLSV LTTYGVHAIT RLEAWSLALL ALFLVLFVAI 550 560 570 580 590 600 VLTIWRQPQN QQKVAFMVPF LPFLPAFSIL VNIYLMVQLS ADTWVRFSIW MAIGFLIYFS 610 620 630 640 650 YGIRHSLEGH LRDENNEEDA YPDNVHAAAE EKSAIQANDH HPRNLSSPFI FHEKTSEF
	Isoform 2 (CAT-2A) [UniParc]. Checksum: A5753DDB8FF1FFFA	FASTA	697	76,024
	10 20 30 40 50 60 MKIETSGYNS DKLICRGFIG TPAPPVCDSK FLLSPSSDVR MIPCRAALTF ARCLIRRKIV 70 80 90 100 110 120 TLDSLEDTKL CRCLSTMDLI ALGVGSTLGA GVYVLAGEVA KADSGPSIVV SFLIAALASV 130 140 150 160 170 180 MAGLCYAEFG ARVPKTGSAY LYTYVTVGEL WAFITGWNLI LSYVIGTSSV ARAWSGTFDE 190 200 210 220 230 240 LLSKQIGQFL RTYFRMNYTG LAEYPDFFAV CLILLLAGLL SFGVKESAWV NKVFTAVNIL 250 260 270 280 290 300 VLLFVMVAGF VKGNVANWKI SEEFLKNISA SAREPPSENG TSIYGAGGFM PYGFTGTLAG 310 320 330 340 350 360 AATCFYAFVG FDCIATTGEE VRNPQKAIPI GIVTSLLVCF MAYFGVSAAL TLMMPYYLLD 370 380 390 400 410 420 EKSPLPVAFE YVGWGPAKYV VAAGSLCALS TSLLGSMFPL PRILFAMARD GLLFRFLARV 430 440 450 460 470 480 SKRQSPVAAT LTAGVISALM AFLFDLKALV DMMSIGTLMA YSLVAACVLI LRYQPGLSYD 490 500 510 520 530 540 QPKCSPEKDG LGSSPRVTSK SESQVTMLQR QGFSMRTLFC PSLLPTQQSA SLVSFLVGFL 550 560 570 580 590 600 AFLVLGLSVL TTYGVHAITR LEAWSLALLA LFLVLFVAIV LTIWRQPQNQ QKVAFMVPFL 610 620 630 640 650 660 PFLPAFSILV NIYLMVQLSA DTWVRFSIWM AIGFLIYFSY GIRHSLEGHL RDENNEEDAY 670 680 690 PDNVHAAAEE KSAIQANDHH PRNLSSPFIF HEKTSEF
	Isoform 3 [UniParc]. Checksum: 6ED2F36C607015C0	FASTA	698	75,986
	10 20 30 40 50 60 MKIETSGYNS DKLICRGFIG TPAPPVCDSK FLLSPSSDVR MIPCRAALTF ARCLIRRKIV 70 80 90 100 110 120 TLDSLEDTKL CRCLSTMDLI ALGVGSTLGA GVYVLAGEVA KADSGPSIVV SFLIAALASV 130 140 150 160 170 180 MAGLCYAEFG ARVPKTGSAY LYTYVTVGEL WAFITGWNLI LSYVIGTSSV ARAWSGTFDE 190 200 210 220 230 240 LLSKQIGQFL RTYFRMNYTG LAEYPDFFAV CLILLLAGLL SFGVKESAWV NKVFTAVNIL 250 260 270 280 290 300 VLLFVMVAGF VKGNVANWKI SEEFLKNISA SAREPPSENG TSIYGAGGFM PYGFTGTLAG 310 320 330 340 350 360 AATCFYAFVG FDCIATTGEE VRNPQKAIPI GIVTSLLVCF MAYFGVSAAL TLMMPYYLLD 370 380 390 400 410 420 EKSPLPVAFE YVGWGPAKYV VAAGSLCALS TSLLGSIFPM PRVIYAMAED GLLFKCLAQI 430 440 450 460 470 480 NSKTKTPIIA TLSSGAVAAL MAFLFDLKAL VDMMSIGTLM AYSLVAACVL ILRYQPGLSY 490 500 510 520 530 540 DQPKCSPEKD GLGSSPRVTS KSESQVTMLQ RQGFSMRTLF CPSLLPTQQS ASLVSFLVGF 550 560 570 580 590 600 LAFLVLGLSV LTTYGVHAIT RLEAWSLALL ALFLVLFVAI VLTIWRQPQN QQKVAFMVPF 610 620 630 640 650 660 LPFLPAFSIL VNIYLMVQLS ADTWVRFSIW MAIGFLIYFS YGIRHSLEGH LRDENNEEDA 670 680 690 YPDNVHAAAE EKSAIQANDH HPRNLSSPFI FHEKTSEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, tissue distribution, and chromosomal localization of human cationic amino acid transporter 2 (HCAT2)." Hoshide R., Ikeda Y., Karashima S., Matsuura T., Komaki S., Kishino T., Niikawa N., Endo F., Matsuda I. Genomics 38:174-178(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Intestine.
[2]	"Human cationic amino acid transporters hCAT-1, hCAT-2A, and hCAT-2B: three related carriers with distinct transport properties." Closs E.I., Graef P., Habermeier A., Cunningham J.M., Foerstermann U. Biochemistry 36:6462-6468(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA] OF 337-469 (ISOFORM 1), FUNCTION, VARIANT THR-531. Tissue: Liver.
[3]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANT THR-531. Tissue: Liver.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 313-658 (ISOFORM 2). Tissue: Skeletal muscle.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-646, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-455, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D29990 mRNA. Translation: BAA06271.1. U76368 mRNA. Translation: AAB62810.1. Frameshift. U76369 mRNA. Translation: AAB62811.1. AB020863 Genomic DNA. No translation available. CH471080 Genomic DNA. Translation: EAW63813.1. BC069648 mRNA. Translation: AAH69648.1. Different initiation. BC104905 mRNA. Translation: AAI04906.1. Different initiation. BC113661 mRNA. Translation: AAI13662.1. Different initiation. BC143583 mRNA. Translation: AAI43584.1. Different initiation. AL832016 mRNA. Translation: CAD89909.1.
IPI	IPI00003819. IPI00873323. IPI00930563.
RefSeq	NP_001008539.3. NM_001008539.3. NP_001158243.1. NM_001164771.1. NP_003037.4. NM_003046.5.
UniGene	Hs.448520.
3D structure databases
ProteinModelPortal	P52569.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52569. 1 interaction.
PTM databases
PhosphoSite	P52569.
Polymorphism databases
DMDM	126302539.
Proteomic databases
PaxDb	P52569.
PRIDE	P52569.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000004531; ENSP00000004531; ENSG00000003989. ENST00000398090; ENSP00000381164; ENSG00000003989. ENST00000470360; ENSP00000419873; ENSG00000003989. ENST00000494857; ENSP00000419140; ENSG00000003989. ENST00000522656; ENSP00000430464; ENSG00000003989.
GeneID	6542.
KEGG	hsa:6542.
UCSC	uc011kyc.2. human. uc011kyd.2. human. uc011kye.2. human.
Organism-specific databases
CTD	6542.
GeneCards	GC08P017354.
HGNC	HGNC:11060. SLC7A2.
HPA	HPA009169.
MIM	601872. gene.
neXtProt	NX_P52569.
PharmGKB	PA35920.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0531.
HOGENOM	HOG000250623.
HOVERGEN	HBG000280.
KO	K13864.
OMA	NSDKLIC.
OrthoDB	EOG46143S.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_19419. Amino acid and oligopeptide SLC transporters.
Gene expression databases
Bgee	P52569.
CleanEx	HS_SLC7A2.
Genevestigator	P52569.
GermOnline	ENSG00000003989. Homo sapiens.
Family and domain databases
InterPro	IPR002293. AA/rel_permease1. IPR015606. Cat-AATrans. IPR004755. Cat_AA_permease. [Graphical view]
PANTHER	PTHR11785. PTHR11785. 1 hit. PTHR11785:SF53. PTHR11785:SF53. 1 hit.
PIRSF	PIRSF006060. AA_transporter. 1 hit.
TIGRFAMs	TIGR00906. 2A0303. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	SLC7A2. human.
DrugBank	DB00123. L-Lysine. DB00129. L-Ornithine.
GenomeRNAi	6542.
NextBio	25455.
SOURCE	Search...

Entry information

Entry name

CTR2_HUMAN

Accession

Primary (citable) accession number: P52569
Secondary accession number(s): B7ZL54 , O15291, O15292, Q14CQ6, Q6NSZ7, Q86TC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	February 20, 2007
Last modified:	April 3, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.