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Protein

Rho GDP-dissociation inhibitor 2

Gene

ARHGDIB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).3 Publications

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB-KW
  • GTPase activity Source: CACAO
  • Rac GTPase binding Source: UniProtKB
  • Rho GDP-dissociation inhibitor activity Source: UniProtKB

GO - Biological processi

  • cellular response to redox state Source: Ensembl
  • movement of cell or subcellular component Source: UniProtKB
  • multicellular organism development Source: ProtInc
  • negative regulation of cell adhesion Source: ProtInc
  • negative regulation of trophoblast cell migration Source: CACAO
  • regulation of actin cytoskeleton reorganization Source: UniProtKB
  • regulation of Rho protein signal transduction Source: UniProtKB
  • regulation of small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.
SIGNORiP52566.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GDP-dissociation inhibitor 2
Short name:
Rho GDI 2
Alternative name(s):
Ly-GDI1 Publication
Rho-GDI beta
Gene namesi
Name:ARHGDIB
Synonyms:GDIA2, GDID4, RAP1GN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:679. ARHGDIB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoplasmic, membrane-bounded vesicle Source: ProtInc
  • cytoskeleton Source: UniProtKB
  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24964.

Polymorphism and mutation databases

BioMutaiARHGDIB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 201200Rho GDP-dissociation inhibitor 2PRO_0000219016Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine1 Publication
Modified residuei21 – 211N6-acetyllysineCombined sources
Modified residuei24 – 241PhosphotyrosineCombined sources
Modified residuei25 – 251N6-acetyllysineCombined sources
Modified residuei40 – 401N6-acetyllysineCombined sources
Modified residuei47 – 471N6-acetyllysineCombined sources
Modified residuei102 – 1021N6-acetyllysineCombined sources
Modified residuei124 – 1241N6-acetyllysineCombined sources
Modified residuei145 – 1451PhosphoserineCombined sources
Modified residuei175 – 1751N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP52566.
MaxQBiP52566.
PaxDbiP52566.
PeptideAtlasiP52566.
PRIDEiP52566.
TopDownProteomicsiP52566.

2D gel databases

OGPiP52566.
SWISS-2DPAGEP52566.

PTM databases

iPTMnetiP52566.
PhosphoSiteiP52566.
SwissPalmiP52566.

Miscellaneous databases

PMAP-CutDBP52566.

Expressioni

Tissue specificityi

Detected in bone marrow, thymus and spleen.1 Publication

Gene expression databases

BgeeiENSG00000111348.
CleanExiHS_ARHGDIB.
ExpressionAtlasiP52566. baseline and differential.
GenevisibleiP52566. HS.

Organism-specific databases

HPAiCAB018584.
HPA051235.

Interactioni

Subunit structurei

Interacts with RHOA (PubMed:20400958). Interacts with RAC1 (PubMed:7512369). Interacts with RAC2 (PubMed:10655614). Interacts with CDC42 (PubMed:7512369).3 Publications

GO - Molecular functioni

  • Rac GTPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi106890. 13 interactions.
DIPiDIP-40959N.
IntActiP52566. 8 interactions.
MINTiMINT-1616342.
STRINGi9606.ENSP00000228945.

Structurei

Secondary structure

1
201
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi32 – 376Combined sources
Turni38 – 414Combined sources
Helixi43 – 5210Combined sources
Beta strandi62 – 643Combined sources
Beta strandi69 – 757Combined sources
Beta strandi84 – 863Combined sources
Helixi91 – 966Combined sources
Beta strandi98 – 1025Combined sources
Beta strandi106 – 1138Combined sources
Beta strandi120 – 13112Combined sources
Beta strandi134 – 14613Combined sources
Beta strandi153 – 1564Combined sources
Turni166 – 1683Combined sources
Beta strandi170 – 17910Combined sources
Beta strandi187 – 19812Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DS6X-ray2.35B23-199[»]
ProteinModelPortaliP52566.
SMRiP52566. Positions 23-199.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52566.

Family & Domainsi

Sequence similaritiesi

Belongs to the Rho GDI family.Curated

Phylogenomic databases

eggNOGiKOG3205. Eukaryota.
ENOG4111K44. LUCA.
GeneTreeiENSGT00390000006233.
HOGENOMiHOG000175765.
HOVERGENiHBG000206.
InParanoidiP52566.
KOiK12462.
OMAiQDHLTWE.
OrthoDBiEOG091G0MBU.
PhylomeDBiP52566.
TreeFamiTF105387.

Family and domain databases

Gene3Di2.70.50.30. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR000406. Rho_GDI.
IPR024792. RhoGDI_domain.
[Graphical view]
PANTHERiPTHR10980. PTHR10980. 1 hit.
PfamiPF02115. Rho_GDI. 1 hit.
[Graphical view]
PRINTSiPR00492. RHOGDI.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEKAPEPHV EEDDDDELDS KLNYKPPPQK SLKELQEMDK DDESLIKYKK
60 70 80 90 100
TLLGDGPVVT DPKAPNVVVT RLTLVCESAP GPITMDLTGD LEALKKETIV
110 120 130 140 150
LKEGSEYRVK IHFKVNRDIV SGLKYVQHTY RTGVKVDKAT FMVGSYGPRP
160 170 180 190 200
EEYEFLTPVE EAPKGMLARG TYHNKSFFTD DDKQDHLSWE WNLSIKKEWT

E
Length:201
Mass (Da):22,988
Last modified:January 23, 2007 - v3
Checksum:iF1E840134F643E5F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 1531Y → V AA sequence (PubMed:2226408).Curated
Sequence conflicti169 – 1702RG → QD in L07916 (PubMed:8434008).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20688 mRNA. Translation: AAA59539.1.
X69549 mRNA. Translation: CAA49280.1.
L07916 mRNA. No translation available.
AF498927 mRNA. Translation: AAM21075.1.
AB451315 mRNA. Translation: BAG70129.1.
AB451445 mRNA. Translation: BAG70259.1.
CH471094 Genomic DNA. Translation: EAW96336.1.
BC009200 mRNA. Translation: AAH09200.1.
CCDSiCCDS8671.1.
PIRiA47742.
RefSeqiNP_001166.3. NM_001175.6.
NP_001308349.1. NM_001321420.1.
NP_001308350.1. NM_001321421.1.
NP_001308351.1. NM_001321422.1.
NP_001308352.1. NM_001321423.1.
UniGeneiHs.504877.

Genome annotation databases

EnsembliENST00000228945; ENSP00000228945; ENSG00000111348.
ENST00000541546; ENSP00000440560; ENSG00000111348.
ENST00000541644; ENSP00000444860; ENSG00000111348.
GeneIDi397.
KEGGihsa:397.
UCSCiuc001rcq.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20688 mRNA. Translation: AAA59539.1.
X69549 mRNA. Translation: CAA49280.1.
L07916 mRNA. No translation available.
AF498927 mRNA. Translation: AAM21075.1.
AB451315 mRNA. Translation: BAG70129.1.
AB451445 mRNA. Translation: BAG70259.1.
CH471094 Genomic DNA. Translation: EAW96336.1.
BC009200 mRNA. Translation: AAH09200.1.
CCDSiCCDS8671.1.
PIRiA47742.
RefSeqiNP_001166.3. NM_001175.6.
NP_001308349.1. NM_001321420.1.
NP_001308350.1. NM_001321421.1.
NP_001308351.1. NM_001321422.1.
NP_001308352.1. NM_001321423.1.
UniGeneiHs.504877.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DS6X-ray2.35B23-199[»]
ProteinModelPortaliP52566.
SMRiP52566. Positions 23-199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106890. 13 interactions.
DIPiDIP-40959N.
IntActiP52566. 8 interactions.
MINTiMINT-1616342.
STRINGi9606.ENSP00000228945.

PTM databases

iPTMnetiP52566.
PhosphoSiteiP52566.
SwissPalmiP52566.

Polymorphism and mutation databases

BioMutaiARHGDIB.

2D gel databases

OGPiP52566.
SWISS-2DPAGEP52566.

Proteomic databases

EPDiP52566.
MaxQBiP52566.
PaxDbiP52566.
PeptideAtlasiP52566.
PRIDEiP52566.
TopDownProteomicsiP52566.

Protocols and materials databases

DNASUi397.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000228945; ENSP00000228945; ENSG00000111348.
ENST00000541546; ENSP00000440560; ENSG00000111348.
ENST00000541644; ENSP00000444860; ENSG00000111348.
GeneIDi397.
KEGGihsa:397.
UCSCiuc001rcq.2. human.

Organism-specific databases

CTDi397.
GeneCardsiARHGDIB.
H-InvDBHIX0036989.
HGNCiHGNC:679. ARHGDIB.
HPAiCAB018584.
HPA051235.
MIMi602843. gene.
neXtProtiNX_P52566.
PharmGKBiPA24964.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3205. Eukaryota.
ENOG4111K44. LUCA.
GeneTreeiENSGT00390000006233.
HOGENOMiHOG000175765.
HOVERGENiHBG000206.
InParanoidiP52566.
KOiK12462.
OMAiQDHLTWE.
OrthoDBiEOG091G0MBU.
PhylomeDBiP52566.
TreeFamiTF105387.

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.
SIGNORiP52566.

Miscellaneous databases

ChiTaRSiARHGDIB. human.
EvolutionaryTraceiP52566.
GeneWikiiARHGDIB.
GenomeRNAii397.
PMAP-CutDBP52566.
PROiP52566.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111348.
CleanExiHS_ARHGDIB.
ExpressionAtlasiP52566. baseline and differential.
GenevisibleiP52566. HS.

Family and domain databases

Gene3Di2.70.50.30. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR000406. Rho_GDI.
IPR024792. RhoGDI_domain.
[Graphical view]
PANTHERiPTHR10980. PTHR10980. 1 hit.
PfamiPF02115. Rho_GDI. 1 hit.
[Graphical view]
PRINTSiPR00492. RHOGDI.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGDIR2_HUMAN
AccessioniPrimary (citable) accession number: P52566
Secondary accession number(s): B5BU79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 159 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.