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P52560

- RELA_STRCO

UniProt

P52560 - RELA_STRCO

Protein

GTP pyrophosphokinase

Gene

relA

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp. PppGpp could play an essential role in triggering antibiotic production under some nutritional conditions.

    Catalytic activityi

    ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

    Pathwayi

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. GTP binding Source: UniProtKB-KW
    4. GTP diphosphokinase activity Source: UniProtKB-EC
    5. kinase activity Source: UniProtKB-KW
    6. metal ion binding Source: InterPro
    7. phosphoric diester hydrolase activity Source: InterPro

    GO - Biological processi

    1. guanosine tetraphosphate biosynthetic process Source: CACAO
    2. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    ATP-binding, GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00908; UER00884.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP pyrophosphokinase (EC:2.7.6.5)
    Alternative name(s):
    (p)ppGpp synthase
    ATP:GTP 3'-pyrophosphotransferase
    ppGpp synthase I
    Gene namesi
    Name:relA
    Ordered Locus Names:SCO1513
    ORF Names:SCL2.03c
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 847847GTP pyrophosphokinasePRO_0000166564Add
    BLAST

    Expressioni

    Inductioni

    By amino acid starvation. Activation through the binding of uncharged tRNA in the acceptor sites of translating ribosomes By similarity.By similarity

    Interactioni

    Protein-protein interaction databases

    STRINGi100226.SCO1513.

    Structurei

    3D structure databases

    ProteinModelPortaliP52560.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini149 – 24698HDAdd
    BLAST
    Domaini768 – 84275ACTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RelA/SpoT family.Curated
    Contains 1 ACT domain.PROSITE-ProRule annotation
    Contains 1 HD domain.Curated

    Phylogenomic databases

    eggNOGiCOG0317.
    HOGENOMiHOG000018301.
    KOiK00951.
    OMAiIGYITRG.
    OrthoDBiEOG6SV551.
    PhylomeDBiP52560.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view]
    PfamiPF01966. HD. 1 hit.
    PF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    SM00954. RelA_SpoT. 1 hit.
    [Graphical view]
    SUPFAMiSSF81271. SSF81271. 1 hit.
    TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
    PROSITEiPS51671. ACT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52560-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPDEAQPLTA AKPESASASA AKPAPSAPQA KNDTHGPIQH APAAPVDKPA    50
    EQQPRPKPLP AERPQNAPVV RAPAGQPARS GSSNRVRARL ARLGVQRANP 100
    YNPVLEPLLR IVRGNDPKIE TSTLRQIERA YQVAERWHRG QKRKSGDPYI 150
    THPLAVTTIL AELGMDPATL MAGLLHDTVE DTEYGLEDLR RDFGDVVTLL 200
    VDGVTKLDKV KFGEAAQAET VRKMVVAMAK DPRVLVIKLA DRLHNMRTMR 250
    YLKREKQEKK ARETLEIYAP LAHRLGMNTI KWELEDLAFA ILYPKMYDEI 300
    VRLVAERAPK RDEYLAVVTD EVQQDLRAAR IKATVTGRPK HYYSVYQKMI 350
    VRGRDFAEIY DLVGIRVLVD TVRDCYAALG TVHARWNPVP GRFKDYIAMP 400
    KFNMYQSLHT TVIGPGGKPV ELQIRTFDMH RRAEYGIAAH WKYKQEAVAG 450
    ASKVRTDAPK SSGKSKDDHL NDMAWLRQLL DWQKETEDPG EFLESLRFDL 500
    SRNEVFVFTP KGDVIALPAG ATPVDFAYAV HTEVGHRTIG ARVNGRLVPL 550
    ESTLDNGDLV EVFTSKAAGA GPSRDWLGFV KSPRARNKIR AWFSKERRDE 600
    AIEQGKDAIV RAMRKQNLPI QRILTGDSLV TLAHEMRYSD ISALYAAIGE 650
    GHVSAPNIVQ KLVQALGGEE AATEEIDESV PPSRGRGRKR RANADPGVVV 700
    KGVEDVWVKL ARCCTPVPGD PIIGFVTRGS GVSVHRSDCV NVDSLSREPE 750
    RILEVEWAPT QSSVFLVAIQ VEALDRSRLL SDVTRVLSDQ HVNILSAAVQ 800
    TSRDRVATSR FTFEMGDPKH LGHVLKAVRG VEGVYDVYRV TSARRPS 847
    Length:847
    Mass (Da):94,182
    Last modified:October 1, 1996 - v1
    Checksum:i544040B21A0D512E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti197 – 1971V → L in CAA63297. (PubMed:8631867)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87267 Genomic DNA. Translation: CAA60717.1.
    X92520 Genomic DNA. Translation: CAA63297.1.
    AL939109 Genomic DNA. Translation: CAB70915.1.
    PIRiS70687.
    RefSeqiNP_625792.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB70915; CAB70915; CAB70915.
    GeneIDi1096939.
    KEGGisco:SCO1513.
    PATRICi23732602. VBIStrCoe124346_1522.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87267 Genomic DNA. Translation: CAA60717.1 .
    X92520 Genomic DNA. Translation: CAA63297.1 .
    AL939109 Genomic DNA. Translation: CAB70915.1 .
    PIRi S70687.
    RefSeqi NP_625792.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali P52560.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO1513.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB70915 ; CAB70915 ; CAB70915 .
    GeneIDi 1096939.
    KEGGi sco:SCO1513.
    PATRICi 23732602. VBIStrCoe124346_1522.

    Phylogenomic databases

    eggNOGi COG0317.
    HOGENOMi HOG000018301.
    KOi K00951.
    OMAi IGYITRG.
    OrthoDBi EOG6SV551.
    PhylomeDBi P52560.

    Enzyme and pathway databases

    UniPathwayi UPA00908 ; UER00884 .

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view ]
    Pfami PF01966. HD. 1 hit.
    PF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    SM00954. RelA_SpoT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81271. SSF81271. 1 hit.
    TIGRFAMsi TIGR00691. spoT_relA. 1 hit.
    PROSITEi PS51671. ACT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2)."
      Chakraburtty R., White J., Takano E., Bibb M.J.
      Mol. Microbiol. 19:357-368(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.
    2. "A relA/spoT homologous gene from Streptomyces coelicolor A3(2) controls antibiotic biosynthetic genes."
      Martinez-Costa O.H., Arias P., Romero N.M., Parro V., Mellado R.P., Malpartida F.
      J. Biol. Chem. 271:10627-10634(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A3(2) / J802.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.

    Entry informationi

    Entry nameiRELA_STRCO
    AccessioniPrimary (citable) accession number: P52560
    Secondary accession number(s): P72401
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3