P52560 (RELA_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP pyrophosphokinase
EC=2.7.6.5

Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I

Gene names

Name:	relA
Ordered Locus Names:	SCO1513
ORF Names:	SCL2.03c

Organism

Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]

Taxonomic identifier

100226 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces coelicolor

Protein attributes

Sequence length	847 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp. PppGpp could play an essential role in triggering antibiotic production under some nutritional conditions.
Catalytic activity	ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.
Induction	By amino acid starvation. Activation through the binding of uncharged tRNA in the acceptor sites of translating ribosomes By similarity.
Sequence similarities	Belongs to the RelA/SpoT family. Contains 1 ACT domain. Contains 1 HD domain.

Ontologies

Keywords
Biological process	Stress response
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP diphosphokinase activity Inferred from electronic annotation. Source: EC kinase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 847	847	GTP pyrophosphokinase		PRO_0000166564
Regions
	Domain	149 – 246	98	HD
	Domain	767 – 840	74	ACT
Experimental info
	Sequence conflict	197	1	V → L in CAA63297. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P52560 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 544040B21A0D512E	FASTA	847	94,182
	10 20 30 40 50 60 MPDEAQPLTA AKPESASASA AKPAPSAPQA KNDTHGPIQH APAAPVDKPA EQQPRPKPLP 70 80 90 100 110 120 AERPQNAPVV RAPAGQPARS GSSNRVRARL ARLGVQRANP YNPVLEPLLR IVRGNDPKIE 130 140 150 160 170 180 TSTLRQIERA YQVAERWHRG QKRKSGDPYI THPLAVTTIL AELGMDPATL MAGLLHDTVE 190 200 210 220 230 240 DTEYGLEDLR RDFGDVVTLL VDGVTKLDKV KFGEAAQAET VRKMVVAMAK DPRVLVIKLA 250 260 270 280 290 300 DRLHNMRTMR YLKREKQEKK ARETLEIYAP LAHRLGMNTI KWELEDLAFA ILYPKMYDEI 310 320 330 340 350 360 VRLVAERAPK RDEYLAVVTD EVQQDLRAAR IKATVTGRPK HYYSVYQKMI VRGRDFAEIY 370 380 390 400 410 420 DLVGIRVLVD TVRDCYAALG TVHARWNPVP GRFKDYIAMP KFNMYQSLHT TVIGPGGKPV 430 440 450 460 470 480 ELQIRTFDMH RRAEYGIAAH WKYKQEAVAG ASKVRTDAPK SSGKSKDDHL NDMAWLRQLL 490 500 510 520 530 540 DWQKETEDPG EFLESLRFDL SRNEVFVFTP KGDVIALPAG ATPVDFAYAV HTEVGHRTIG 550 560 570 580 590 600 ARVNGRLVPL ESTLDNGDLV EVFTSKAAGA GPSRDWLGFV KSPRARNKIR AWFSKERRDE 610 620 630 640 650 660 AIEQGKDAIV RAMRKQNLPI QRILTGDSLV TLAHEMRYSD ISALYAAIGE GHVSAPNIVQ 670 680 690 700 710 720 KLVQALGGEE AATEEIDESV PPSRGRGRKR RANADPGVVV KGVEDVWVKL ARCCTPVPGD 730 740 750 760 770 780 PIIGFVTRGS GVSVHRSDCV NVDSLSREPE RILEVEWAPT QSSVFLVAIQ VEALDRSRLL 790 800 810 820 830 840 SDVTRVLSDQ HVNILSAAVQ TSRDRVATSR FTFEMGDPKH LGHVLKAVRG VEGVYDVYRV TSARRPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2)." Chakraburtty R., White J., Takano E., Bibb M.J. Mol. Microbiol. 19:357-368(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[2]	"A relA/spoT homologous gene from Streptomyces coelicolor A3(2) controls antibiotic biosynthetic genes." Martinez-Costa O.H., Arias P., Romero N.M., Parro V., Mellado R.P., Malpartida F. J. Biol. Chem. 271:10627-10634(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3(2) / J802.
[3]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87267 Genomic DNA. Translation: CAA60717.1. X92520 Genomic DNA. Translation: CAA63297.1. AL939109 Genomic DNA. Translation: CAB70915.1.
PIR	S70687.
RefSeq	NP_625792.1. NC_003888.3.
3D structure databases
ProteinModelPortal	P52560.
ModBase	Search...
Protein-protein interaction databases
STRING	100226.SCO1513.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB70915; CAB70915; CAB70915.
GeneID	1096939.
KEGG	sco:SCO1513.
PATRIC	23732602. VBIStrCoe124346_1522.
Phylogenomic databases
eggNOG	COG0317.
HOGENOM	HOG000018301.
KO	K00951.
OMA	LSWFREI.
ProtClustDB	CLSK812689.
Enzyme and pathway databases
UniPathway	UPA00908; UER00884.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR026020. (p)ppGpp_Synthase. IPR012675. Beta-grasp_dom. IPR003607. HD/PDEase_dom. IPR006674. HD_domain. IPR004811. RelA/Spo_fam. IPR007685. RelA_SpoT. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
PANTHER	PTHR21262. PTHR21262. 1 hit.
Pfam	PF01966. HD. 1 hit. PF04607. RelA_SpoT. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. SM00954. RelA_SpoT. 1 hit. [Graphical view]
SUPFAM	SSF81271. TGS-like. 1 hit.
TIGRFAMs	TIGR00691. spoT_relA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RELA_STRCO

Accession

Primary (citable) accession number: P52560
Secondary accession number(s): P72401

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program