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P52560 (RELA_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GTP pyrophosphokinase

EC=2.7.6.5
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene names
Name:relA
Ordered Locus Names:SCO1513
ORF Names:SCL2.03c
OrganismStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]
Taxonomic identifier100226 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group

Protein attributes

Sequence length847 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp. PppGpp could play an essential role in triggering antibiotic production under some nutritional conditions.

Catalytic activity

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathway

Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.

Induction

By amino acid starvation. Activation through the binding of uncharged tRNA in the acceptor sites of translating ribosomes By similarity.

Sequence similarities

Belongs to the RelA/SpoT family.

Contains 1 ACT domain.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 847847GTP pyrophosphokinase
PRO_0000166564

Regions

Domain149 – 24698HD
Domain768 – 84275ACT

Experimental info

Sequence conflict1971V → L in CAA63297. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P52560 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 544040B21A0D512E

FASTA84794,182
        10         20         30         40         50         60 
MPDEAQPLTA AKPESASASA AKPAPSAPQA KNDTHGPIQH APAAPVDKPA EQQPRPKPLP 

        70         80         90        100        110        120 
AERPQNAPVV RAPAGQPARS GSSNRVRARL ARLGVQRANP YNPVLEPLLR IVRGNDPKIE 

       130        140        150        160        170        180 
TSTLRQIERA YQVAERWHRG QKRKSGDPYI THPLAVTTIL AELGMDPATL MAGLLHDTVE 

       190        200        210        220        230        240 
DTEYGLEDLR RDFGDVVTLL VDGVTKLDKV KFGEAAQAET VRKMVVAMAK DPRVLVIKLA 

       250        260        270        280        290        300 
DRLHNMRTMR YLKREKQEKK ARETLEIYAP LAHRLGMNTI KWELEDLAFA ILYPKMYDEI 

       310        320        330        340        350        360 
VRLVAERAPK RDEYLAVVTD EVQQDLRAAR IKATVTGRPK HYYSVYQKMI VRGRDFAEIY 

       370        380        390        400        410        420 
DLVGIRVLVD TVRDCYAALG TVHARWNPVP GRFKDYIAMP KFNMYQSLHT TVIGPGGKPV 

       430        440        450        460        470        480 
ELQIRTFDMH RRAEYGIAAH WKYKQEAVAG ASKVRTDAPK SSGKSKDDHL NDMAWLRQLL 

       490        500        510        520        530        540 
DWQKETEDPG EFLESLRFDL SRNEVFVFTP KGDVIALPAG ATPVDFAYAV HTEVGHRTIG 

       550        560        570        580        590        600 
ARVNGRLVPL ESTLDNGDLV EVFTSKAAGA GPSRDWLGFV KSPRARNKIR AWFSKERRDE 

       610        620        630        640        650        660 
AIEQGKDAIV RAMRKQNLPI QRILTGDSLV TLAHEMRYSD ISALYAAIGE GHVSAPNIVQ 

       670        680        690        700        710        720 
KLVQALGGEE AATEEIDESV PPSRGRGRKR RANADPGVVV KGVEDVWVKL ARCCTPVPGD 

       730        740        750        760        770        780 
PIIGFVTRGS GVSVHRSDCV NVDSLSREPE RILEVEWAPT QSSVFLVAIQ VEALDRSRLL 

       790        800        810        820        830        840 
SDVTRVLSDQ HVNILSAAVQ TSRDRVATSR FTFEMGDPKH LGHVLKAVRG VEGVYDVYRV 


TSARRPS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2)."
Chakraburtty R., White J., Takano E., Bibb M.J.
Mol. Microbiol. 19:357-368(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC BAA-471 / A3(2) / M145.
[2]"A relA/spoT homologous gene from Streptomyces coelicolor A3(2) controls antibiotic biosynthetic genes."
Martinez-Costa O.H., Arias P., Romero N.M., Parro V., Mellado R.P., Malpartida F.
J. Biol. Chem. 271:10627-10634(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A3(2) / J802.
[3]"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)."
Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. expand/collapse author list , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.
Nature 417:141-147(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X87267 Genomic DNA. Translation: CAA60717.1.
X92520 Genomic DNA. Translation: CAA63297.1.
AL939109 Genomic DNA. Translation: CAB70915.1.
PIRS70687.
RefSeqNP_625792.1. NC_003888.3.

3D structure databases

ProteinModelPortalP52560.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING100226.SCO1513.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB70915; CAB70915; CAB70915.
GeneID1096939.
KEGGsco:SCO1513.
PATRIC23732602. VBIStrCoe124346_1522.

Phylogenomic databases

eggNOGCOG0317.
HOGENOMHOG000018301.
KOK00951.
OMAIGYITRG.
OrthoDBEOG6SV551.
PhylomeDBP52560.

Enzyme and pathway databases

UniPathwayUPA00908; UER00884.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamPF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMSSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00691. spoT_relA. 1 hit.
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRELA_STRCO
AccessionPrimary (citable) accession number: P52560
Secondary accession number(s): P72401
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways