P52559 (PURK_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthase
Short name=N5-CAIR synthase
EC=6.3.4.18

Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetase

Gene names

Name:	purK
Ordered Locus Names:	BMEI0295

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) By similarity.
Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.
Sequence caution	The sequence AAA57003.1 differs from that shown. Reason: Frameshift at positions 22, 43, 63, 67, 140 and 150.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	5-(carboxyamino)imidazole ribonucleotide synthase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	N5-carboxyaminoimidazole ribonucleotide synthase		PRO_0000074995
Regions
	Domain	112 – 300	189	ATP-grasp
	Nucleotide binding	185 – 188	4	ATP By similarity
	Nucleotide binding	270 – 271	2	ATP By similarity
Sites
	Binding site	108	1	ATP By similarity
	Binding site	148	1	ATP By similarity
	Binding site	159	1	ATP By similarity
	Binding site	193	1	ATP By similarity
Experimental info
	Sequence conflict	41	1	Q → H Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52559 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 536A73DC33014F47	FASTA	362	38,682
	10 20 30 40 50 60 MDKTSLKPGS TIGIIGGGQL GRMLAMAAAR FGYETIILEP QAGCPAAQVA NRQIVAAYDD 70 80 90 100 110 120 PKALAELAAA SDVITYEFEN VPVSAADKLA ETALVLPPPA ALEISQDRFT EKQFLNESGI 130 140 150 160 170 180 ETAPWRLVDD EETLIAALGA LGGRGILKIR RLGYDGKGQV RLASLDETQA CNAFAAINKA 190 200 210 220 230 240 PAILEGFVEF EREVSVIAAR DRSGNVAIFD LAENVHKDGI LATSTVPAAI SVQTAEAART 250 260 270 280 290 300 AAEKLLHALD YVGVLGLEFF VLKDGTLLAN EFAPRVHNSG HWTEAACAIS QFEQHIRAVA 310 320 330 340 350 360 GLPLGNTDRH SDCVMENLIG DDIEKVPAIL CEKNAVLHLY GKKEARAGRK IGHVTRIKPR TI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and genetic characterization of the purEK operon of Brucella melitensis strain 16M." Warren R., Hoover D., Hadfield T., Drazek S. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.
[2]	"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10241 Unassigned DNA. Translation: AAA57003.1. Frameshift. AE008917 Genomic DNA. Translation: AAL51476.1.
PIR	AI3288.
RefSeq	NP_539212.1. NC_003317.1.
3D structure databases
ProteinModelPortal	P52559.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1196006.
GenomeReviews	Gene locus BMEI0295 in contig AE008917_GR.
KEGG	bme:BMEI0295.
NMPDR	fig|224914.1.peg.294.
PATRIC	17802231. VBIBruMel146950_3432.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG516369.
OMA	GRKMGHF.
PhylomeDB	P52559.
ProtClustDB	PRK06019.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0295-MONOMER.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01589.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR01161. PurK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_BRUME

Accession

Primary (citable) accession number: P52559

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2002
Last modified:	January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program