P52558 (PURE_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N5-carboxyaminoimidazole ribonucleotide mutase
Short name=N5-CAIR mutase
EC=5.4.99.18

Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide mutase

Gene names

Name:	purE
Ordered Locus Names:	BMEI0296

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR) By similarity.
Catalytic activity	5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the AIR carboxylase family.
Sequence caution	The sequence AAL51477.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	5-(carboxyamino)imidazole ribonucleotide mutase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 162	162	N5-carboxyaminoimidazole ribonucleotide mutase		PRO_0000074970
Sites
	Binding site	11	1	Substrate By similarity
	Binding site	14	1	Substrate By similarity
	Binding site	41	1	Substrate By similarity
Experimental info
	Sequence conflict	55	1	A → R in AAA57002. Ref.1
	Sequence conflict	67 – 68	2	GA → R in AAA57002. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52558 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 0B812230C4EE9EE7	FASTA	162	16,596
	10 20 30 40 50 60 MSVDVAIIMG SQSDWETMHH AADTLEALGI SFDARIVSAH RTPDRLVAFA KGAKAEGFKV 70 80 90 100 110 120 IIAGAGGAAH LPGMAAAMTP LPVFGVPVQS KALSGQDSLL SIVQMPAGIP VGTLAIGRAG 130 140 150 160 AVNAALLAAA VLALYDEALA ARLDEWRKAQ TESVAERPSN EA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and genetic characterization of the purEK operon of Brucella melitensis strain 16M." Warren R., Hoover D., Hadfield T., Drazek S. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.
[2]	"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10241 Unassigned DNA. Translation: AAA57002.1. AE008917 Genomic DNA. Translation: AAL51477.1. Different initiation.
PIR	AB3289.
RefSeq	NP_539213.1. NC_003317.1.
3D structure databases
ProteinModelPortal	P52558.
SMR	P52558. Positions 5-161.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1196007.
GenomeReviews	Gene locus BMEI0296 in contig AE008917_GR.
KEGG	bme:BMEI0296.
PATRIC	17850606. VBIBruMel92729_0325.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG301965.
OMA	LIMGSDS.
PhylomeDB	P52558.
ProtClustDB	CLSK898071.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0296-MONOMER.
Family and domain databases
InterPro	IPR024694. N5-CAIR_mutase_PurE. IPR000031. N5-CAIR_Mutase_PurE_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit.
KO	K01588.
Pfam	PF00731. AIRC. 1 hit. [Graphical view]
PIRSF	PIRSF001338. AIR_carboxylase. 1 hit.
SMART	SM01001. AIRC. 1 hit. [Graphical view]
SUPFAM	SSF52255. AIR_carboxyl. 1 hit.
TIGRFAMs	TIGR01162. PurE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURE_BRUME

Accession

Primary (citable) accession number: P52558

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2002
Last modified:	January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program