Reviewed, UniProtKB/Swiss-Prot P52557 (CARA_BACCL)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase pyrimidine-specific small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:

pyrAA

Organism

Bacillus caldolyticus

Taxonomic identifier

1394 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Carbamoyl-phosphate synthase pyrimidine-specific small chain HAMAP MF_01209		PRO_0000112248
Regions
	Domain	171 – 358	188	Glutamine amidotransferase type-1
	Region	1 – 167	167	CPSase HAMAP MF_01209
Sites
	Active site	246	1	Nucleophile By similarity
	Active site	331	1	By similarity
	Active site	333	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52557-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 383A54A839F6631F	FASTA	364	40,289
	10 20 30 40 50 60 MKRQLILEDG SFFVGERFGS LKETTGEVVF NTGMTGYQEI LSDPSYCGQI VTMTYPLIGN 70 80 90 100 110 120 YGINRDDFEA IRPHVHGFIV KEACVKPSNW RGELTLDEYL KEKGIPGLSG IDTRKLTRLI 130 140 150 160 170 180 RQYGTLKGMI CGLDVDPVEA AAKLRAMEWP RDQVRRVSTK SAYPSPGRGE RIVLIDFGMK 190 200 210 220 230 240 HGILRELNKR NCDVIVLPYN ATAEEVLGWH PDGVMLSNGP GDPKDVPEAI EMIRGILGKV 250 260 270 280 290 300 PLFGICLGHQ LFALACGANT EKMKFGHRGS NHPVKDLRTG KVAITSQNHG YTVTHESLSG 310 320 330 340 350 360 TRLEVTHIAL NDGTVEGLRH LDVPAFTVQY HPEASPGPED ANPLFDEFLA LIREFNKKGE VIHA

References

[1]

"The pyrimidine biosynthesis operon of the thermophile Bacillus caldolyticus includes genes for uracil phosphoribosyltransferase and uracil permease." Ghim S.Y., Neuhard J. J. Bacteriol. 176:3698-3707(1994) [PubMed: 8206848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 405 / IFO 15313 / YP-T.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X73308 Genomic DNA. Translation: CAA51738.1.
PIR	I40168.
3D structure databases
HSSP	HSSP built from PDB template 1A9X based on UniProtKB P00907.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.5.5. 1670.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_BACCL

Accession

Primary (citable) accession number: P52557

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	September 22, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)