Reviewed, UniProtKB/Swiss-Prot P52556 (BLVRB_BOVIN)

Names and origin

Protein names

Recommended name:
    Flavin reductase
      Short name=FR
    EC=1.5.1.30
Alternative name(s):
    NADPH-dependent diaphorase
    NADPH-flavin reductase
      Short name=FLR
    Biliverdin reductase B
      Short name=BVR-B
    EC=1.3.1.24
    Biliverdin-IX beta-reductase
    Green heme-binding protein
      Short name=GHBP

Gene names

Name:

BLVRB

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes electron transfer from reduced pyridine nucleotides to flavins as well as methylene blue, pyrroloquinoline quinone, riboflavin, or methemoglobin. Possible role in protecting cells from oxidative damage or in regulating iron metabolism. In the liver, converts biliverdin to bilirubin.
Catalytic activity	Reduced riboflavin + NADP(+) = riboflavin + NADPH. Bilirubin + NAD(P)(+) = biliverdin + NAD(P)H.
Subunit structure	Monomer By similarity.
Subcellular location	CytoplasmPotential.
Tissue specificity	At least expressed in the liver and erythrocyte.
Mass spectrometry	Molecular weight is 21994 Da from positions 2 - 206. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	cellular metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	biliverdin reductase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro flavin reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 206	205	Flavin reductase		PRO_0000064947
Regions
	Nucleotide binding	5 – 36	32	NAD or NADP Potential

Sequences

	Sequence		Length	Mass (Da)
	P52556-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 79A679FED289E32C	FASTA	206	22,132
	10 20 30 40 50 60 MVVKKIALFG ATGNTGLTTL AQAVQAGYEV TVLVRDPSRL PSEGPQPAHV VVGDVRQPAD 70 80 90 100 110 120 VDKTVAGQDA VIVLLGTRND LSPTTVMSEG AQNIVAAMKA HGVDKVVACT SAFLLWDPSK 130 140 150 160 170 180 VPPRLQDVTD DHIRMHKVLQ QSGLKYVAVM PPHIGDHPLT GAYTVTLDGR GPSRVISKHD 190 200 LGHFMLHCLT TDKYDGHTTY PSHVYE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Flavin reductase: sequence of cDNA from bovine liver and tissue distribution." Quandt K.S., Hultquist D.E. Proc. Natl. Acad. Sci. U.S.A. 91:9322-9326(1994) [PubMed: 7937764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 37-58 AND 118-127, MASS SPECTROMETRY. Tissue: Liver.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[3]	"Evidence that the protein components of bovine erythrocyte green heme binding protein and flavin reductase are identical." Quandt K.S., Xu F., Chen P., Hultquist D.E. Biochem. Biophys. Res. Commun. 178:315-321(1991) [PubMed: 2069570] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-37; 121-137 AND 146-169. Tissue: Erythrocyte.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L35045 mRNA. Translation: AAC37323.1. BC102269 mRNA. Translation: AAI02270.1.
PIR	S68597.
RefSeq	NP_776676.1.
UniGene	Bt.4093
3D structure databases
HSSP	HSSP built from PDB template 1HDO based on UniProtKB P30043.
SMR	P52556. Positions 1-205.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000010508. Bos taurus. [Contig view]
GeneID	281650.
KEGG	bta:281650.
Phylogenomic databases
HOVERGEN	P52556.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLVRB_BOVIN

Accession

Primary (citable) accession number: P52556
Secondary accession number(s): Q3T0T4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)