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P52496

- DNLI4_CANAL

UniProt

P52496 - DNLI4_CANAL

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Protein

DNA ligase 4

Gene

LIG4

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in ds DNA break (DSB) repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (NHEJ). Not required for the repair of DSBs induced by ionizing radiation or UV light. Has a important role in morphogenesis, positively affecting the capacity to form hyphae.3 Publications

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).PROSITE-ProRule annotation

Cofactori

Magnesium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei302 – 3021ATPBy similarity
Active sitei304 – 3041N6-AMP-lysine intermediatePROSITE-ProRule annotation
Binding sitei309 – 3091ATPBy similarity
Binding sitei324 – 3241ATPBy similarity
Metal bindingi362 – 3621Magnesium 1Sequence Analysis
Metal bindingi469 – 4691Magnesium 2Sequence Analysis
Binding sitei474 – 4741ATPBy similarity
Binding sitei486 – 4861ATPBy similarity
Binding sitei492 – 4921ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-EC
  4. DNA ligase activity Source: CGD
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA biosynthetic process Source: InterPro
  2. DNA ligation involved in DNA repair Source: InterPro
  3. DNA recombination Source: UniProtKB-KW
  4. DNA replication Source: UniProtKB-KW
  5. filamentous growth Source: CGD
  6. filamentous growth of a population of unicellular organisms Source: CGD
  7. pathogenesis Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1)
Short name:
CaLIG4
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:LIG4
Synonyms:CDC9
ORF Names:CaO19.13220, CaO19.5798
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0001558. LIG4.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 928928DNA ligase 4PRO_0000059584Add
BLAST

Expressioni

Inductioni

Cell cycle-regulated. Expression peaks in late G1 and during the morphological transition.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi5476.CAL0001558.

Structurei

3D structure databases

ProteinModelPortaliP52496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini673 – 76997BRCT 1PROSITE-ProRule annotationAdd
BLAST
Domaini821 – 927107BRCT 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated
Contains 2 BRCT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1793.
InParanoidiP52496.
KOiK10777.
OrthoDBiEOG7QC84G.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR10459:SF7. PTHR10459:SF7. 1 hit.
PfamiPF00533. BRCT. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52496-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTYFLNDIRP PSPNDITPSF TLLTKELFDK LDGVRKESLG DFRTVTEKKA
60 70 80 90 100
FIIKTFINTF RTHIGNDIYP SAKLIFPEKS GRIYFIKEVA LARLLIKMYK
110 120 130 140 150
IPKESEDYIT LHDWNKSYQR SRRFSIDEKK IRDLPLQASR IISKRRPIVD
160 170 180 190 200
KLEEYTVPQI NSSLDQLALE KVSQGQIDIL KPLFDNLSIP EVRWLIHILL
210 220 230 240 250
NKSILTSMER FFFNTWHPDG YRVFSICNDL QKTLQFSTNP DLRLDPSQLA
260 270 280 290 300
IHPCFKFKPQ LSERLTTSYK TLVKKLQRKH EMDPPYEKKF QELGLENKFY
310 320 330 340 350
IEEKMDGDRM LLHKDGDSFK FFSRRLKDYS YLYGESFQFG ALTKFLAHAF
360 370 380 390 400
AGNIQSVILD GEMVAYDYER NVILPFGTLK SSAIQESVRQ FTTIDQYEQQ
410 420 430 440 450
TAYPFFLVFD ILFLNGKDLT NYPLFFRKNI LNRILRPIPN RFEVLDTRLG
460 470 480 490 500
SSSEDIERAI REVVSSRCEG LVLKNVQSKY EIDGFRNPDW IKVKPEYLEK
510 520 530 540 550
FGENLDLVVI GKSPAIKNSY MCGLKSVTDG VYYSFCTCAN GIEIEEFDKI
560 570 580 590 600
ERLTHGKWIK TDVAMPPESL IKFGTKIPTF WIHPSDSLVL EIRARSIDTR
610 620 630 640 650
AGTSYAVGST LHNNHCRKIR EDKSIDECVT LQEYTHIKAN YINDLNKAQT
660 670 680 690 700
ALGKKREPVY SLDNESKLKK VKVESDLFSG IEFLIMSDKR EADGEVTTIE
710 720 730 740 750
EMKAMVKQYG GKIVNSVDLA TNYQIMVITE RELPVSSQYL SKGIDLVKPI
760 770 780 790 800
WIYECIKRGC VLQLEPYFIF ASKNWDNFNH MVDQYGDSYI IHHPLNIVVP
810 820 830 840 850
KLSESELEDL RNGFDWGDLK PWIYLFKGLS FYVCGNNLSA RFLKERIERF
860 870 880 890 900
SGDLSKHFIE CCYIVIPDNH SRPLMLREID KMSNQISREM VIDENGGSSR
910 920
IPHFVTEAFV QASIKMNYIP DPDDYKFR
Length:928
Mass (Da):107,997
Last modified:September 2, 2008 - v3
Checksum:iE15D5151593E462E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti531 – 5344VYYS → FIIV in CAA64457. (PubMed:8840507)Curated
Sequence conflicti564 – 5641A → S in CAA64457. (PubMed:8840507)Curated
Sequence conflicti698 – 6981T → R in CAA64457. (PubMed:8840507)Curated
Sequence conflicti894 – 8941E → K in CAA64457. (PubMed:8840507)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X95001 Genomic DNA. Translation: CAA64457.2.
AACQ01000088 Genomic DNA. Translation: EAK96245.1.
AACQ01000087 Genomic DNA. Translation: EAK96312.1.
RefSeqiXP_715274.1. XM_710181.1.
XP_715339.1. XM_710246.1.

Genome annotation databases

GeneIDi3642986.
3643043.
KEGGical:CaO19.13220.
cal:CaO19.5798.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X95001 Genomic DNA. Translation: CAA64457.2 .
AACQ01000088 Genomic DNA. Translation: EAK96245.1 .
AACQ01000087 Genomic DNA. Translation: EAK96312.1 .
RefSeqi XP_715274.1. XM_710181.1.
XP_715339.1. XM_710246.1.

3D structure databases

ProteinModelPortali P52496.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0001558.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3642986.
3643043.
KEGGi cal:CaO19.13220.
cal:CaO19.5798.

Organism-specific databases

CGDi CAL0001558. LIG4.

Phylogenomic databases

eggNOGi COG1793.
InParanoidi P52496.
KOi K10777.
OrthoDBi EOG7QC84G.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
InterProi IPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
PANTHERi PTHR10459:SF7. PTHR10459:SF7. 1 hit.
Pfami PF00533. BRCT. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SMARTi SM00292. BRCT. 2 hits.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A Candida albicans gene encoding a DNA ligase."
    Andaluz E., Larriba G., Calderone R.
    Yeast 12:893-898(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 56884 / 366.
  2. Larriba G.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  4. "Cell cycle regulation of a DNA ligase-encoding gene (CaLIG4) from Candida albicans."
    Andaluz E., Ciudad A., Rubio Coque J., Calderone R., Larriba G.
    Yeast 15:1199-1210(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  5. "Phenotypic analysis and virulence of Candida albicans LIG4 mutants."
    Andaluz E., Calderone R., Reyes G., Larriba G.
    Infect. Immun. 69:137-147(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "An evaluation of the role of LIG4 in genomic instability and adaptive mutagenesis in Candida albicans."
    Andaluz E., Ciudad T., Larriba G.
    FEMS Yeast Res. 2:341-348(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDNLI4_CANAL
AccessioniPrimary (citable) accession number: P52496
Secondary accession number(s): Q5A0L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 2, 2008
Last modified: October 29, 2014
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3