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P52496

- DNLI4_CANAL

UniProt

P52496 - DNLI4_CANAL

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Protein
DNA ligase 4
Gene
LIG4, CDC9, CaO19.13220, CaO19.5798
Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in ds DNA break (DSB) repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (NHEJ). Not required for the repair of DSBs induced by ionizing radiation or UV light. Has a important role in morphogenesis, positively affecting the capacity to form hyphae.3 Publications

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactori

Magnesium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei302 – 3021ATP By similarity
Active sitei304 – 3041N6-AMP-lysine intermediate By similarity
Binding sitei309 – 3091ATP By similarity
Binding sitei324 – 3241ATP By similarity
Metal bindingi362 – 3621Magnesium 1 Reviewed prediction
Metal bindingi469 – 4691Magnesium 2 Reviewed prediction
Binding sitei474 – 4741ATP By similarity
Binding sitei486 – 4861ATP By similarity
Binding sitei492 – 4921ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-EC
  4. DNA ligase activity Source: CGD
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA ligation involved in DNA repair Source: InterPro
  2. DNA recombination Source: UniProtKB-KW
  3. DNA replication Source: UniProtKB-KW
  4. filamentous growth Source: CGD
  5. filamentous growth of a population of unicellular organisms Source: CGD
  6. pathogenesis Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1)
Short name:
CaLIG4
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:LIG4
Synonyms:CDC9
ORF Names:CaO19.13220, CaO19.5798
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0001558. LIG4.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: CGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 928928DNA ligase 4
PRO_0000059584Add
BLAST

Expressioni

Inductioni

Cell cycle-regulated. Expression peaks in late G1 and during the morphological transition.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi5476.CAL0001558.

Structurei

3D structure databases

ProteinModelPortaliP52496.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini673 – 76997BRCT 1
Add
BLAST
Domaini821 – 927107BRCT 2
Add
BLAST

Sequence similaritiesi

Contains 2 BRCT domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1793.
KOiK10777.
OrthoDBiEOG7QC84G.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52496-1 [UniParc]FASTAAdd to Basket

« Hide

MTYFLNDIRP PSPNDITPSF TLLTKELFDK LDGVRKESLG DFRTVTEKKA    50
FIIKTFINTF RTHIGNDIYP SAKLIFPEKS GRIYFIKEVA LARLLIKMYK 100
IPKESEDYIT LHDWNKSYQR SRRFSIDEKK IRDLPLQASR IISKRRPIVD 150
KLEEYTVPQI NSSLDQLALE KVSQGQIDIL KPLFDNLSIP EVRWLIHILL 200
NKSILTSMER FFFNTWHPDG YRVFSICNDL QKTLQFSTNP DLRLDPSQLA 250
IHPCFKFKPQ LSERLTTSYK TLVKKLQRKH EMDPPYEKKF QELGLENKFY 300
IEEKMDGDRM LLHKDGDSFK FFSRRLKDYS YLYGESFQFG ALTKFLAHAF 350
AGNIQSVILD GEMVAYDYER NVILPFGTLK SSAIQESVRQ FTTIDQYEQQ 400
TAYPFFLVFD ILFLNGKDLT NYPLFFRKNI LNRILRPIPN RFEVLDTRLG 450
SSSEDIERAI REVVSSRCEG LVLKNVQSKY EIDGFRNPDW IKVKPEYLEK 500
FGENLDLVVI GKSPAIKNSY MCGLKSVTDG VYYSFCTCAN GIEIEEFDKI 550
ERLTHGKWIK TDVAMPPESL IKFGTKIPTF WIHPSDSLVL EIRARSIDTR 600
AGTSYAVGST LHNNHCRKIR EDKSIDECVT LQEYTHIKAN YINDLNKAQT 650
ALGKKREPVY SLDNESKLKK VKVESDLFSG IEFLIMSDKR EADGEVTTIE 700
EMKAMVKQYG GKIVNSVDLA TNYQIMVITE RELPVSSQYL SKGIDLVKPI 750
WIYECIKRGC VLQLEPYFIF ASKNWDNFNH MVDQYGDSYI IHHPLNIVVP 800
KLSESELEDL RNGFDWGDLK PWIYLFKGLS FYVCGNNLSA RFLKERIERF 850
SGDLSKHFIE CCYIVIPDNH SRPLMLREID KMSNQISREM VIDENGGSSR 900
IPHFVTEAFV QASIKMNYIP DPDDYKFR 928
Length:928
Mass (Da):107,997
Last modified:September 2, 2008 - v3
Checksum:iE15D5151593E462E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti531 – 5344VYYS → FIIV in CAA64457. 1 Publication
Sequence conflicti564 – 5641A → S in CAA64457. 1 Publication
Sequence conflicti698 – 6981T → R in CAA64457. 1 Publication
Sequence conflicti894 – 8941E → K in CAA64457. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X95001 Genomic DNA. Translation: CAA64457.2.
AACQ01000088 Genomic DNA. Translation: EAK96245.1.
AACQ01000087 Genomic DNA. Translation: EAK96312.1.
RefSeqiXP_715274.1. XM_710181.1.
XP_715339.1. XM_710246.1.

Genome annotation databases

GeneIDi3642986.
3643043.
KEGGical:CaO19.13220.
cal:CaO19.5798.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X95001 Genomic DNA. Translation: CAA64457.2 .
AACQ01000088 Genomic DNA. Translation: EAK96245.1 .
AACQ01000087 Genomic DNA. Translation: EAK96312.1 .
RefSeqi XP_715274.1. XM_710181.1.
XP_715339.1. XM_710246.1.

3D structure databases

ProteinModelPortali P52496.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0001558.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3642986.
3643043.
KEGGi cal:CaO19.13220.
cal:CaO19.5798.

Organism-specific databases

CGDi CAL0001558. LIG4.

Phylogenomic databases

eggNOGi COG1793.
KOi K10777.
OrthoDBi EOG7QC84G.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
InterProi IPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
Pfami PF00533. BRCT. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SMARTi SM00292. BRCT. 2 hits.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A Candida albicans gene encoding a DNA ligase."
    Andaluz E., Larriba G., Calderone R.
    Yeast 12:893-898(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 56884 / 366.
  2. Larriba G.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  4. "Cell cycle regulation of a DNA ligase-encoding gene (CaLIG4) from Candida albicans."
    Andaluz E., Ciudad A., Rubio Coque J., Calderone R., Larriba G.
    Yeast 15:1199-1210(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  5. "Phenotypic analysis and virulence of Candida albicans LIG4 mutants."
    Andaluz E., Calderone R., Reyes G., Larriba G.
    Infect. Immun. 69:137-147(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "An evaluation of the role of LIG4 in genomic instability and adaptive mutagenesis in Candida albicans."
    Andaluz E., Ciudad T., Larriba G.
    FEMS Yeast Res. 2:341-348(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDNLI4_CANAL
AccessioniPrimary (citable) accession number: P52496
Secondary accession number(s): Q5A0L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 2, 2008
Last modified: June 11, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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