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P52496

- DNLI4_CANAL

UniProt

P52496 - DNLI4_CANAL

Protein

DNA ligase 4

Gene

LIG4

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 3 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Involved in ds DNA break (DSB) repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (NHEJ). Not required for the repair of DSBs induced by ionizing radiation or UV light. Has a important role in morphogenesis, positively affecting the capacity to form hyphae.3 Publications

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).PROSITE-ProRule annotation

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei302 – 3021ATPBy similarity
    Active sitei304 – 3041N6-AMP-lysine intermediatePROSITE-ProRule annotation
    Binding sitei309 – 3091ATPBy similarity
    Binding sitei324 – 3241ATPBy similarity
    Metal bindingi362 – 3621Magnesium 1Sequence Analysis
    Metal bindingi469 – 4691Magnesium 2Sequence Analysis
    Binding sitei474 – 4741ATPBy similarity
    Binding sitei486 – 4861ATPBy similarity
    Binding sitei492 – 4921ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: UniProtKB-EC
    4. DNA ligase activity Source: CGD
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA ligation involved in DNA repair Source: InterPro
    2. DNA recombination Source: UniProtKB-KW
    3. DNA replication Source: UniProtKB-KW
    4. filamentous growth Source: CGD
    5. filamentous growth of a population of unicellular organisms Source: CGD
    6. pathogenesis Source: CGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase 4 (EC:6.5.1.1)
    Short name:
    CaLIG4
    Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4
    Gene namesi
    Name:LIG4
    Synonyms:CDC9
    ORF Names:CaO19.13220, CaO19.5798
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0001558. LIG4.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: CGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 928928DNA ligase 4PRO_0000059584Add
    BLAST

    Expressioni

    Inductioni

    Cell cycle-regulated. Expression peaks in late G1 and during the morphological transition.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi5476.CAL0001558.

    Structurei

    3D structure databases

    ProteinModelPortaliP52496.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini673 – 76997BRCT 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini821 – 927107BRCT 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.Curated
    Contains 2 BRCT domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1793.
    KOiK10777.
    OrthoDBiEOG7QC84G.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.40.50.10190. 1 hit.
    InterProiIPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF00533. BRCT. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00292. BRCT. 2 hits.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 1 hit.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS50172. BRCT. 1 hit.
    PS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52496-1 [UniParc]FASTAAdd to Basket

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    MTYFLNDIRP PSPNDITPSF TLLTKELFDK LDGVRKESLG DFRTVTEKKA    50
    FIIKTFINTF RTHIGNDIYP SAKLIFPEKS GRIYFIKEVA LARLLIKMYK 100
    IPKESEDYIT LHDWNKSYQR SRRFSIDEKK IRDLPLQASR IISKRRPIVD 150
    KLEEYTVPQI NSSLDQLALE KVSQGQIDIL KPLFDNLSIP EVRWLIHILL 200
    NKSILTSMER FFFNTWHPDG YRVFSICNDL QKTLQFSTNP DLRLDPSQLA 250
    IHPCFKFKPQ LSERLTTSYK TLVKKLQRKH EMDPPYEKKF QELGLENKFY 300
    IEEKMDGDRM LLHKDGDSFK FFSRRLKDYS YLYGESFQFG ALTKFLAHAF 350
    AGNIQSVILD GEMVAYDYER NVILPFGTLK SSAIQESVRQ FTTIDQYEQQ 400
    TAYPFFLVFD ILFLNGKDLT NYPLFFRKNI LNRILRPIPN RFEVLDTRLG 450
    SSSEDIERAI REVVSSRCEG LVLKNVQSKY EIDGFRNPDW IKVKPEYLEK 500
    FGENLDLVVI GKSPAIKNSY MCGLKSVTDG VYYSFCTCAN GIEIEEFDKI 550
    ERLTHGKWIK TDVAMPPESL IKFGTKIPTF WIHPSDSLVL EIRARSIDTR 600
    AGTSYAVGST LHNNHCRKIR EDKSIDECVT LQEYTHIKAN YINDLNKAQT 650
    ALGKKREPVY SLDNESKLKK VKVESDLFSG IEFLIMSDKR EADGEVTTIE 700
    EMKAMVKQYG GKIVNSVDLA TNYQIMVITE RELPVSSQYL SKGIDLVKPI 750
    WIYECIKRGC VLQLEPYFIF ASKNWDNFNH MVDQYGDSYI IHHPLNIVVP 800
    KLSESELEDL RNGFDWGDLK PWIYLFKGLS FYVCGNNLSA RFLKERIERF 850
    SGDLSKHFIE CCYIVIPDNH SRPLMLREID KMSNQISREM VIDENGGSSR 900
    IPHFVTEAFV QASIKMNYIP DPDDYKFR 928
    Length:928
    Mass (Da):107,997
    Last modified:September 2, 2008 - v3
    Checksum:iE15D5151593E462E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti531 – 5344VYYS → FIIV in CAA64457. (PubMed:8840507)Curated
    Sequence conflicti564 – 5641A → S in CAA64457. (PubMed:8840507)Curated
    Sequence conflicti698 – 6981T → R in CAA64457. (PubMed:8840507)Curated
    Sequence conflicti894 – 8941E → K in CAA64457. (PubMed:8840507)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95001 Genomic DNA. Translation: CAA64457.2.
    AACQ01000088 Genomic DNA. Translation: EAK96245.1.
    AACQ01000087 Genomic DNA. Translation: EAK96312.1.
    RefSeqiXP_715274.1. XM_710181.1.
    XP_715339.1. XM_710246.1.

    Genome annotation databases

    GeneIDi3642986.
    3643043.
    KEGGical:CaO19.13220.
    cal:CaO19.5798.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95001 Genomic DNA. Translation: CAA64457.2 .
    AACQ01000088 Genomic DNA. Translation: EAK96245.1 .
    AACQ01000087 Genomic DNA. Translation: EAK96312.1 .
    RefSeqi XP_715274.1. XM_710181.1.
    XP_715339.1. XM_710246.1.

    3D structure databases

    ProteinModelPortali P52496.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5476.CAL0001558.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3642986.
    3643043.
    KEGGi cal:CaO19.13220.
    cal:CaO19.5798.

    Organism-specific databases

    CGDi CAL0001558. LIG4.

    Phylogenomic databases

    eggNOGi COG1793.
    KOi K10777.
    OrthoDBi EOG7QC84G.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.40.50.10190. 1 hit.
    InterProi IPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF00533. BRCT. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00292. BRCT. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 1 hit.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS50172. BRCT. 1 hit.
    PS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A Candida albicans gene encoding a DNA ligase."
      Andaluz E., Larriba G., Calderone R.
      Yeast 12:893-898(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 56884 / 366.
    2. Larriba G.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    4. "Cell cycle regulation of a DNA ligase-encoding gene (CaLIG4) from Candida albicans."
      Andaluz E., Ciudad A., Rubio Coque J., Calderone R., Larriba G.
      Yeast 15:1199-1210(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    5. "Phenotypic analysis and virulence of Candida albicans LIG4 mutants."
      Andaluz E., Calderone R., Reyes G., Larriba G.
      Infect. Immun. 69:137-147(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "An evaluation of the role of LIG4 in genomic instability and adaptive mutagenesis in Candida albicans."
      Andaluz E., Ciudad T., Larriba G.
      FEMS Yeast Res. 2:341-348(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiDNLI4_CANAL
    AccessioniPrimary (citable) accession number: P52496
    Secondary accession number(s): Q5A0L3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 95 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3