DNA ligase 4 - Candida albicans (Yeast)

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Reviewed, UniProtKB/Swiss-Prot P52496 (DNLI4_CANAL)

Last modified June 16, 2009. Version 57. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    DNA ligase 4
      Short name=CaLIG4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	LIG4
Synonyms:	CDC9
ORF Names:	CaO19.5798, CaO19.13220

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

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Protein attributes

Sequence length	928 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Involved in ds DNA break (DSB) repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (NHEJ). Not required for the repair of DSBs induced by ionizing radiation or UV light. Has a important role in morphogenesis, positively affecting the capacity to form hyphae. Ref.4 Ref.5 Ref.6
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus.
Induction	Cell cycle-regulated. Expression peaks in late G1 and during the morphological transition. Ref.4
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 1 BRCT domain.

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Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 928

928

DNA ligase 4

PRO_0000059584

Regions

Domain

673 – 769

BRCT 1

Domain

821 – 927

107

BRCT 2

Sites

Active site

304

N6-AMP-lysine intermediate By similarity

Metal binding

362

Magnesium 1 Potential

Metal binding

469

Magnesium 2 Potential

Binding site

302

ATP By similarity

Binding site

309

ATP By similarity

Binding site

324

ATP By similarity

Binding site

474

ATP By similarity

Binding site

486

ATP By similarity

Binding site

492

ATP By similarity

Experimental info

Sequence conflict

531 – 534

VYYS → FIIV in CAA64457. Ref.1

Sequence conflict

564

A → S in CAA64457. Ref.1

Sequence conflict

698

T → R in CAA64457. Ref.1

Sequence conflict

894

E → K in CAA64457. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P52496-1 [UniParc].

Last modified September 2, 2008. Version 3.
Checksum: E15D5151593E462E
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FASTA

928

107,997

        10         20         30         40         50         60 
MTYFLNDIRP PSPNDITPSF TLLTKELFDK LDGVRKESLG DFRTVTEKKA FIIKTFINTF 

        70         80         90        100        110        120 
RTHIGNDIYP SAKLIFPEKS GRIYFIKEVA LARLLIKMYK IPKESEDYIT LHDWNKSYQR 

       130        140        150        160        170        180 
SRRFSIDEKK IRDLPLQASR IISKRRPIVD KLEEYTVPQI NSSLDQLALE KVSQGQIDIL 

       190        200        210        220        230        240 
KPLFDNLSIP EVRWLIHILL NKSILTSMER FFFNTWHPDG YRVFSICNDL QKTLQFSTNP 

       250        260        270        280        290        300 
DLRLDPSQLA IHPCFKFKPQ LSERLTTSYK TLVKKLQRKH EMDPPYEKKF QELGLENKFY 

       310        320        330        340        350        360 
IEEKMDGDRM LLHKDGDSFK FFSRRLKDYS YLYGESFQFG ALTKFLAHAF AGNIQSVILD 

       370        380        390        400        410        420 
GEMVAYDYER NVILPFGTLK SSAIQESVRQ FTTIDQYEQQ TAYPFFLVFD ILFLNGKDLT 

       430        440        450        460        470        480 
NYPLFFRKNI LNRILRPIPN RFEVLDTRLG SSSEDIERAI REVVSSRCEG LVLKNVQSKY 

       490        500        510        520        530        540 
EIDGFRNPDW IKVKPEYLEK FGENLDLVVI GKSPAIKNSY MCGLKSVTDG VYYSFCTCAN 

       550        560        570        580        590        600 
GIEIEEFDKI ERLTHGKWIK TDVAMPPESL IKFGTKIPTF WIHPSDSLVL EIRARSIDTR 

       610        620        630        640        650        660 
AGTSYAVGST LHNNHCRKIR EDKSIDECVT LQEYTHIKAN YINDLNKAQT ALGKKREPVY 

       670        680        690        700        710        720 
SLDNESKLKK VKVESDLFSG IEFLIMSDKR EADGEVTTIE EMKAMVKQYG GKIVNSVDLA 

       730        740        750        760        770        780 
TNYQIMVITE RELPVSSQYL SKGIDLVKPI WIYECIKRGC VLQLEPYFIF ASKNWDNFNH 

       790        800        810        820        830        840 
MVDQYGDSYI IHHPLNIVVP KLSESELEDL RNGFDWGDLK PWIYLFKGLS FYVCGNNLSA 

       850        860        870        880        890        900 
RFLKERIERF SGDLSKHFIE CCYIVIPDNH SRPLMLREID KMSNQISREM VIDENGGSSR 

       910        920 
IPHFVTEAFV QASIKMNYIP DPDDYKFR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Candida albicans gene encoding a DNA ligase." Andaluz E., Larriba G., Calderone R. Yeast 12:893-898(1996) [PubMed: 8840507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 56884 / 366.
[2]	Larriba G. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.
[4]	"Cell cycle regulation of a DNA ligase-encoding gene (CaLIG4) from Candida albicans." Andaluz E., Ciudad A., Rubio Coque J., Calderone R., Larriba G. Yeast 15:1199-1210(1999) [PubMed: 10487922] [Abstract] Cited for: FUNCTION, INDUCTION.
[5]	"Phenotypic analysis and virulence of Candida albicans LIG4 mutants." Andaluz E., Calderone R., Reyes G., Larriba G. Infect. Immun. 69:137-147(2001) [PubMed: 11119499] [Abstract] Cited for: FUNCTION.
[6]	"An evaluation of the role of LIG4 in genomic instability and adaptive mutagenesis in Candida albicans." Andaluz E., Ciudad T., Larriba G. FEMS Yeast Res. 2:341-348(2002) [PubMed: 12702284] [Abstract] Cited for: FUNCTION.

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Cross-references

Sequence databases
	X95001 Genomic DNA. Translation: CAA64457.2. AACQ01000088 Genomic DNA. Translation: EAK96245.1. AACQ01000087 Genomic DNA. Translation: EAK96312.1.
RefSeq	XP_715274.1. XP_715339.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3642986. 3643043.
KEGG	cal:CaO19.13220. cal:CaO19.5798.
Organism-specific databases
CGD	CAL0001558. LIG4.
Phylogenomic databases
OMA	P52496. RRLKDYS.
Enzyme and pathway databases
BRENDA	6.5.1.1. 1124.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

DNLI4_CANAL

Accession

Primary (citable) accession number: P52496
Secondary accession number(s): Q5A0L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 2, 2008
Last modified:	June 16, 2009
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

Candida albicans

Candida albicans: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents