Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P52496 (DNLI4_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase 4

Short name=CaLIG4
EC=6.5.1.1
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene names
Name:LIG4
Synonyms:CDC9
ORF Names:CaO19.13220, CaO19.5798
OrganismCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]
Taxonomic identifier237561 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length928 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in ds DNA break (DSB) repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (NHEJ). Not required for the repair of DSBs induced by ionizing radiation or UV light. Has a important role in morphogenesis, positively affecting the capacity to form hyphae. Ref.4 Ref.5 Ref.6

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus.

Induction

Cell cycle-regulated. Expression peaks in late G1 and during the morphological transition. Ref.4

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Contains 2 BRCT domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 928928DNA ligase 4
PRO_0000059584

Regions

Domain673 – 76997BRCT 1
Domain821 – 927107BRCT 2

Sites

Active site3041N6-AMP-lysine intermediate By similarity
Metal binding3621Magnesium 1 Potential
Metal binding4691Magnesium 2 Potential
Binding site3021ATP By similarity
Binding site3091ATP By similarity
Binding site3241ATP By similarity
Binding site4741ATP By similarity
Binding site4861ATP By similarity
Binding site4921ATP By similarity

Experimental info

Sequence conflict531 – 5344VYYS → FIIV in CAA64457. Ref.1
Sequence conflict5641A → S in CAA64457. Ref.1
Sequence conflict6981T → R in CAA64457. Ref.1
Sequence conflict8941E → K in CAA64457. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P52496 [UniParc].

Last modified September 2, 2008. Version 3.
Checksum: E15D5151593E462E

FASTA928107,997
        10         20         30         40         50         60 
MTYFLNDIRP PSPNDITPSF TLLTKELFDK LDGVRKESLG DFRTVTEKKA FIIKTFINTF 

        70         80         90        100        110        120 
RTHIGNDIYP SAKLIFPEKS GRIYFIKEVA LARLLIKMYK IPKESEDYIT LHDWNKSYQR 

       130        140        150        160        170        180 
SRRFSIDEKK IRDLPLQASR IISKRRPIVD KLEEYTVPQI NSSLDQLALE KVSQGQIDIL 

       190        200        210        220        230        240 
KPLFDNLSIP EVRWLIHILL NKSILTSMER FFFNTWHPDG YRVFSICNDL QKTLQFSTNP 

       250        260        270        280        290        300 
DLRLDPSQLA IHPCFKFKPQ LSERLTTSYK TLVKKLQRKH EMDPPYEKKF QELGLENKFY 

       310        320        330        340        350        360 
IEEKMDGDRM LLHKDGDSFK FFSRRLKDYS YLYGESFQFG ALTKFLAHAF AGNIQSVILD 

       370        380        390        400        410        420 
GEMVAYDYER NVILPFGTLK SSAIQESVRQ FTTIDQYEQQ TAYPFFLVFD ILFLNGKDLT 

       430        440        450        460        470        480 
NYPLFFRKNI LNRILRPIPN RFEVLDTRLG SSSEDIERAI REVVSSRCEG LVLKNVQSKY 

       490        500        510        520        530        540 
EIDGFRNPDW IKVKPEYLEK FGENLDLVVI GKSPAIKNSY MCGLKSVTDG VYYSFCTCAN 

       550        560        570        580        590        600 
GIEIEEFDKI ERLTHGKWIK TDVAMPPESL IKFGTKIPTF WIHPSDSLVL EIRARSIDTR 

       610        620        630        640        650        660 
AGTSYAVGST LHNNHCRKIR EDKSIDECVT LQEYTHIKAN YINDLNKAQT ALGKKREPVY 

       670        680        690        700        710        720 
SLDNESKLKK VKVESDLFSG IEFLIMSDKR EADGEVTTIE EMKAMVKQYG GKIVNSVDLA 

       730        740        750        760        770        780 
TNYQIMVITE RELPVSSQYL SKGIDLVKPI WIYECIKRGC VLQLEPYFIF ASKNWDNFNH 

       790        800        810        820        830        840 
MVDQYGDSYI IHHPLNIVVP KLSESELEDL RNGFDWGDLK PWIYLFKGLS FYVCGNNLSA 

       850        860        870        880        890        900 
RFLKERIERF SGDLSKHFIE CCYIVIPDNH SRPLMLREID KMSNQISREM VIDENGGSSR 

       910        920 
IPHFVTEAFV QASIKMNYIP DPDDYKFR 

« Hide

References

« Hide 'large scale' references
[1]"A Candida albicans gene encoding a DNA ligase."
Andaluz E., Larriba G., Calderone R.
Yeast 12:893-898(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 56884 / 366.
[2]Larriba G.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.
[4]"Cell cycle regulation of a DNA ligase-encoding gene (CaLIG4) from Candida albicans."
Andaluz E., Ciudad A., Rubio Coque J., Calderone R., Larriba G.
Yeast 15:1199-1210(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[5]"Phenotypic analysis and virulence of Candida albicans LIG4 mutants."
Andaluz E., Calderone R., Reyes G., Larriba G.
Infect. Immun. 69:137-147(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"An evaluation of the role of LIG4 in genomic instability and adaptive mutagenesis in Candida albicans."
Andaluz E., Ciudad T., Larriba G.
FEMS Yeast Res. 2:341-348(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X95001 Genomic DNA. Translation: CAA64457.2.
AACQ01000088 Genomic DNA. Translation: EAK96245.1.
AACQ01000087 Genomic DNA. Translation: EAK96312.1.
RefSeqXP_715274.1. XM_710181.1.
XP_715339.1. XM_710246.1.

3D structure databases

ProteinModelPortalP52496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5476.CAL0001558.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3642986.
3643043.
KEGGcal:CaO19.13220.
cal:CaO19.5798.

Organism-specific databases

CGDCAL0001558. LIG4.

Phylogenomic databases

eggNOGCOG1793.
KOK10777.
OrthoDBEOG7QC84G.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
InterProIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF00533. BRCT. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI4_CANAL
AccessionPrimary (citable) accession number: P52496
Secondary accession number(s): Q5A0L3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 2, 2008
Last modified: June 11, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Candida albicans

Candida albicans: entries and gene names