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Protein

DNA ligase 4

Gene

LIG4

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in ds DNA break (DSB) repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (NHEJ). Not required for the repair of DSBs induced by ionizing radiation or UV light. Has a important role in morphogenesis, positively affecting the capacity to form hyphae.3 Publications

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.PROSITE-ProRule annotation

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei302ATPBy similarity1
Active sitei304N6-AMP-lysine intermediatePROSITE-ProRule annotation1
Binding sitei309ATPBy similarity1
Binding sitei324ATPBy similarity1
Metal bindingi362Magnesium 1Sequence analysis1
Metal bindingi469Magnesium 2Sequence analysis1
Binding sitei474ATPBy similarity1
Binding sitei486ATPBy similarity1
Binding sitei492ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1PROSITE-ProRule annotation)
Short name:
CaLIG4
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:LIG4
Synonyms:CDC9
ORF Names:CaO19.13220, CaO19.5798
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000595841 – 928DNA ligase 4Add BLAST928

Proteomic databases

PRIDEiP52496.

Expressioni

Inductioni

Cell cycle-regulated. Expression peaks in late G1 and during the morphological transition.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP52496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini673 – 769BRCT 1PROSITE-ProRule annotationAdd BLAST97
Domaini821 – 927BRCT 2PROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated
Contains 2 BRCT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiP52496.
KOiK10777.
OrthoDBiEOG092C18KW.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR10459:SF7. PTHR10459:SF7. 3 hits.
PfamiPF16589. BRCT_2. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52496-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYFLNDIRP PSPNDITPSF TLLTKELFDK LDGVRKESLG DFRTVTEKKA
60 70 80 90 100
FIIKTFINTF RTHIGNDIYP SAKLIFPEKS GRIYFIKEVA LARLLIKMYK
110 120 130 140 150
IPKESEDYIT LHDWNKSYQR SRRFSIDEKK IRDLPLQASR IISKRRPIVD
160 170 180 190 200
KLEEYTVPQI NSSLDQLALE KVSQGQIDIL KPLFDNLSIP EVRWLIHILL
210 220 230 240 250
NKSILTSMER FFFNTWHPDG YRVFSICNDL QKTLQFSTNP DLRLDPSQLA
260 270 280 290 300
IHPCFKFKPQ LSERLTTSYK TLVKKLQRKH EMDPPYEKKF QELGLENKFY
310 320 330 340 350
IEEKMDGDRM LLHKDGDSFK FFSRRLKDYS YLYGESFQFG ALTKFLAHAF
360 370 380 390 400
AGNIQSVILD GEMVAYDYER NVILPFGTLK SSAIQESVRQ FTTIDQYEQQ
410 420 430 440 450
TAYPFFLVFD ILFLNGKDLT NYPLFFRKNI LNRILRPIPN RFEVLDTRLG
460 470 480 490 500
SSSEDIERAI REVVSSRCEG LVLKNVQSKY EIDGFRNPDW IKVKPEYLEK
510 520 530 540 550
FGENLDLVVI GKSPAIKNSY MCGLKSVTDG VYYSFCTCAN GIEIEEFDKI
560 570 580 590 600
ERLTHGKWIK TDVAMPPESL IKFGTKIPTF WIHPSDSLVL EIRARSIDTR
610 620 630 640 650
AGTSYAVGST LHNNHCRKIR EDKSIDECVT LQEYTHIKAN YINDLNKAQT
660 670 680 690 700
ALGKKREPVY SLDNESKLKK VKVESDLFSG IEFLIMSDKR EADGEVTTIE
710 720 730 740 750
EMKAMVKQYG GKIVNSVDLA TNYQIMVITE RELPVSSQYL SKGIDLVKPI
760 770 780 790 800
WIYECIKRGC VLQLEPYFIF ASKNWDNFNH MVDQYGDSYI IHHPLNIVVP
810 820 830 840 850
KLSESELEDL RNGFDWGDLK PWIYLFKGLS FYVCGNNLSA RFLKERIERF
860 870 880 890 900
SGDLSKHFIE CCYIVIPDNH SRPLMLREID KMSNQISREM VIDENGGSSR
910 920
IPHFVTEAFV QASIKMNYIP DPDDYKFR
Length:928
Mass (Da):107,997
Last modified:September 2, 2008 - v3
Checksum:iE15D5151593E462E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti531 – 534VYYS → FIIV in CAA64457 (PubMed:8840507).Curated4
Sequence conflicti564A → S in CAA64457 (PubMed:8840507).Curated1
Sequence conflicti698T → R in CAA64457 (PubMed:8840507).Curated1
Sequence conflicti894E → K in CAA64457 (PubMed:8840507).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95001 Genomic DNA. Translation: CAA64457.2.
AACQ01000088 Genomic DNA. Translation: EAK96245.1.
AACQ01000087 Genomic DNA. Translation: EAK96312.1.
RefSeqiXP_715274.1. XM_710181.1.
XP_715339.1. XM_710246.1.

Genome annotation databases

EnsemblFungiiEAK96245; EAK96245; CaO19.13220.
EAK96312; EAK96312; CaO19.5798.
GeneIDi3642986.
3643043.
KEGGical:CaO19.13220.
cal:CaO19.5798.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95001 Genomic DNA. Translation: CAA64457.2.
AACQ01000088 Genomic DNA. Translation: EAK96245.1.
AACQ01000087 Genomic DNA. Translation: EAK96312.1.
RefSeqiXP_715274.1. XM_710181.1.
XP_715339.1. XM_710246.1.

3D structure databases

ProteinModelPortaliP52496.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP52496.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAK96245; EAK96245; CaO19.13220.
EAK96312; EAK96312; CaO19.5798.
GeneIDi3642986.
3643043.
KEGGical:CaO19.13220.
cal:CaO19.5798.

Phylogenomic databases

InParanoidiP52496.
KOiK10777.
OrthoDBiEOG092C18KW.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR10459:SF7. PTHR10459:SF7. 3 hits.
PfamiPF16589. BRCT_2. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNLI4_CANAL
AccessioniPrimary (citable) accession number: P52496
Secondary accession number(s): Q5A0L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 2, 2008
Last modified: November 30, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.