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P52489

- KPYK2_YEAST

UniProt

P52489 - KPYK2_YEAST

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Protein

Pyruvate kinase 2

Gene

PYK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be used by cells under conditions in which the level of glycolytic flux is very low.

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.By similarity
Potassium.By similarity

Enzyme regulationi

Not activated by fructose-1,6-bisphosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511SubstrateBy similarity
Metal bindingi53 – 531PotassiumBy similarity
Metal bindingi55 – 551PotassiumBy similarity
Metal bindingi86 – 861PotassiumBy similarity
Metal bindingi87 – 871Potassium; via carbonyl oxygenBy similarity
Sitei242 – 2421Transition state stabilizerBy similarity
Metal bindingi244 – 2441MagnesiumSequence Analysis
Binding sitei267 – 2671Substrate; via amide nitrogenBy similarity
Metal bindingi268 – 2681MagnesiumBy similarity
Binding sitei268 – 2681Substrate; via amide nitrogenBy similarity
Binding sitei300 – 3001SubstrateBy similarity
Binding sitei339 – 3391ADPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: SGD

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
  2. pyruvate metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciYEAST:YOR347C-MONOMER.
SABIO-RKP52489.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase 2 (EC:2.7.1.40)
Short name:
PK 2
Gene namesi
Name:PYK2
Ordered Locus Names:YOR347C
ORF Names:O6342
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR347c.
SGDiS000005874. PYK2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Pyruvate kinase 2PRO_0000112122Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241PhosphoserineSequence Analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP52489.
PaxDbiP52489.
PeptideAtlasiP52489.

Expressioni

Gene expression databases

GenevestigatoriP52489.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi34732. 25 interactions.
IntActiP52489. 12 interactions.
MINTiMINT-2783027.
STRINGi4932.YOR347C.

Structurei

3D structure databases

ProteinModelPortaliP52489.
SMRiP52489. Positions 4-502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
GeneTreeiENSGT00390000008859.
HOGENOMiHOG000021559.
InParanoidiP52489.
KOiK00873.
OMAiPWILAIS.
OrthoDBiEOG7M6DJC.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52489-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPESRLQRLA NLKIGTPQQL RRTSIIGTIG PKTNSCEAIT ALRKAGLNII
60 70 80 90 100
RLNFSHGSYE FHQSVIENAV KSEQQFPGRP LAIALDTKGP EIRTGRTLND
110 120 130 140 150
QDLYIPVDHQ MIFTTDASFA NTSNDKIMYI DYANLTKVIV PGRFIYVDDG
160 170 180 190 200
ILSFKVLQII DESNLRVQAV NSGYIASHKG VNLPNTDVDL PPLSAKDMKD
210 220 230 240 250
LQFGVRNGIH IVFASFIRTS EDVLSIRKAL GSEGQDIKII SKIENQQGLD
260 270 280 290 300
NFDEILEVTD GVMIARGDLG IEILAPEVLA IQKKLIAKCN LAGKPVICAT
310 320 330 340 350
QMLDSMTHNP RPTRAEVSDV GNAVLDGADC VMLSGETAKG DYPVNAVNIM
360 370 380 390 400
AATALIAEST IAHLALYDDL RDATPKPTST TETVAAAATA AILEQDGKAI
410 420 430 440 450
VVLSTTGNTA RLLSKYRPSC PIILVTRHAR TARIAHLYRG VFPFLYEPKR
460 470 480 490 500
LDDWGEDVHR RLKFGVEMAR SFGMVDNGDT VVSIQGFKGG VGHSNTLRIS

TVGQEF
Length:506
Mass (Da):55,195
Last modified:October 1, 1996 - v1
Checksum:iE0B36C298FA2E5E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X95720 Genomic DNA. Translation: CAA65034.1.
Z75255 Genomic DNA. Translation: CAA99675.1.
BK006948 Genomic DNA. Translation: DAA11108.1.
PIRiS67256.
RefSeqiNP_014992.3. NM_001183767.3.

Genome annotation databases

EnsemblFungiiYOR347C; YOR347C; YOR347C.
GeneIDi854529.
KEGGisce:YOR347C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X95720 Genomic DNA. Translation: CAA65034.1 .
Z75255 Genomic DNA. Translation: CAA99675.1 .
BK006948 Genomic DNA. Translation: DAA11108.1 .
PIRi S67256.
RefSeqi NP_014992.3. NM_001183767.3.

3D structure databases

ProteinModelPortali P52489.
SMRi P52489. Positions 4-502.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34732. 25 interactions.
IntActi P52489. 12 interactions.
MINTi MINT-2783027.
STRINGi 4932.YOR347C.

Proteomic databases

MaxQBi P52489.
PaxDbi P52489.
PeptideAtlasi P52489.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR347C ; YOR347C ; YOR347C .
GeneIDi 854529.
KEGGi sce:YOR347C.

Organism-specific databases

CYGDi YOR347c.
SGDi S000005874. PYK2.

Phylogenomic databases

eggNOGi COG0469.
GeneTreei ENSGT00390000008859.
HOGENOMi HOG000021559.
InParanoidi P52489.
KOi K00873.
OMAi PWILAIS.
OrthoDBi EOG7M6DJC.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
BioCyci YEAST:YOR347C-MONOMER.
SABIO-RK P52489.

Miscellaneous databases

NextBioi 976910.

Gene expression databases

Genevestigatori P52489.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis of a 40 kb segment on the right arm of yeast chromosome XV reveals 18 open reading frames including a new pyruvate kinase and three homologues to chromosome I genes."
    Purnelle B., Goffeau A.
    Yeast 12:1475-1481(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 90843 / S288c / FY73.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate."
    Boles E., Schulte F., Miosga T., Freidel K., Schlueter E., Zimmermann F.K., Hollenberg C.P., Heinisch J.J.
    J. Bacteriol. 179:2987-2993(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKPYK2_YEAST
AccessioniPrimary (citable) accession number: P52489
Secondary accession number(s): D6W342
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2130 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3