Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P52489

- KPYK2_YEAST

UniProt

P52489 - KPYK2_YEAST

Protein

Pyruvate kinase 2

Gene

PYK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May be used by cells under conditions in which the level of glycolytic flux is very low.

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Enzyme regulationi

    Not activated by fructose-1,6-bisphosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei51 – 511SubstrateBy similarity
    Metal bindingi53 – 531PotassiumBy similarity
    Metal bindingi55 – 551PotassiumBy similarity
    Metal bindingi86 – 861PotassiumBy similarity
    Metal bindingi87 – 871Potassium; via carbonyl oxygenBy similarity
    Sitei242 – 2421Transition state stabilizerBy similarity
    Metal bindingi244 – 2441MagnesiumSequence Analysis
    Binding sitei267 – 2671Substrate; via amide nitrogenBy similarity
    Metal bindingi268 – 2681MagnesiumBy similarity
    Binding sitei268 – 2681Substrate; via amide nitrogenBy similarity
    Binding sitei300 – 3001SubstrateBy similarity
    Binding sitei339 – 3391ADPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: SGD

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway
    2. pyruvate metabolic process Source: SGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciYEAST:YOR347C-MONOMER.
    SABIO-RKP52489.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase 2 (EC:2.7.1.40)
    Short name:
    PK 2
    Gene namesi
    Name:PYK2
    Ordered Locus Names:YOR347C
    ORF Names:O6342
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR347c.
    SGDiS000005874. PYK2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 506506Pyruvate kinase 2PRO_0000112122Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei24 – 241PhosphoserineSequence Analysis

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP52489.
    PaxDbiP52489.
    PeptideAtlasiP52489.

    Expressioni

    Gene expression databases

    GenevestigatoriP52489.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    BioGridi34732. 24 interactions.
    IntActiP52489. 12 interactions.
    MINTiMINT-2783027.
    STRINGi4932.YOR347C.

    Structurei

    3D structure databases

    ProteinModelPortaliP52489.
    SMRiP52489. Positions 4-502.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    GeneTreeiENSGT00390000008859.
    HOGENOMiHOG000021559.
    KOiK00873.
    OMAiPWILAIS.
    OrthoDBiEOG7M6DJC.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52489-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPESRLQRLA NLKIGTPQQL RRTSIIGTIG PKTNSCEAIT ALRKAGLNII    50
    RLNFSHGSYE FHQSVIENAV KSEQQFPGRP LAIALDTKGP EIRTGRTLND 100
    QDLYIPVDHQ MIFTTDASFA NTSNDKIMYI DYANLTKVIV PGRFIYVDDG 150
    ILSFKVLQII DESNLRVQAV NSGYIASHKG VNLPNTDVDL PPLSAKDMKD 200
    LQFGVRNGIH IVFASFIRTS EDVLSIRKAL GSEGQDIKII SKIENQQGLD 250
    NFDEILEVTD GVMIARGDLG IEILAPEVLA IQKKLIAKCN LAGKPVICAT 300
    QMLDSMTHNP RPTRAEVSDV GNAVLDGADC VMLSGETAKG DYPVNAVNIM 350
    AATALIAEST IAHLALYDDL RDATPKPTST TETVAAAATA AILEQDGKAI 400
    VVLSTTGNTA RLLSKYRPSC PIILVTRHAR TARIAHLYRG VFPFLYEPKR 450
    LDDWGEDVHR RLKFGVEMAR SFGMVDNGDT VVSIQGFKGG VGHSNTLRIS 500
    TVGQEF 506
    Length:506
    Mass (Da):55,195
    Last modified:October 1, 1996 - v1
    Checksum:iE0B36C298FA2E5E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95720 Genomic DNA. Translation: CAA65034.1.
    Z75255 Genomic DNA. Translation: CAA99675.1.
    BK006948 Genomic DNA. Translation: DAA11108.1.
    PIRiS67256.
    RefSeqiNP_014992.3. NM_001183767.3.

    Genome annotation databases

    EnsemblFungiiYOR347C; YOR347C; YOR347C.
    GeneIDi854529.
    KEGGisce:YOR347C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95720 Genomic DNA. Translation: CAA65034.1 .
    Z75255 Genomic DNA. Translation: CAA99675.1 .
    BK006948 Genomic DNA. Translation: DAA11108.1 .
    PIRi S67256.
    RefSeqi NP_014992.3. NM_001183767.3.

    3D structure databases

    ProteinModelPortali P52489.
    SMRi P52489. Positions 4-502.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34732. 24 interactions.
    IntActi P52489. 12 interactions.
    MINTi MINT-2783027.
    STRINGi 4932.YOR347C.

    Proteomic databases

    MaxQBi P52489.
    PaxDbi P52489.
    PeptideAtlasi P52489.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR347C ; YOR347C ; YOR347C .
    GeneIDi 854529.
    KEGGi sce:YOR347C.

    Organism-specific databases

    CYGDi YOR347c.
    SGDi S000005874. PYK2.

    Phylogenomic databases

    eggNOGi COG0469.
    GeneTreei ENSGT00390000008859.
    HOGENOMi HOG000021559.
    KOi K00873.
    OMAi PWILAIS.
    OrthoDBi EOG7M6DJC.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci YEAST:YOR347C-MONOMER.
    SABIO-RK P52489.

    Miscellaneous databases

    NextBioi 976910.

    Gene expression databases

    Genevestigatori P52489.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence analysis of a 40 kb segment on the right arm of yeast chromosome XV reveals 18 open reading frames including a new pyruvate kinase and three homologues to chromosome I genes."
      Purnelle B., Goffeau A.
      Yeast 12:1475-1481(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 90843 / S288c / FY73.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate."
      Boles E., Schulte F., Miosga T., Freidel K., Schlueter E., Zimmermann F.K., Hollenberg C.P., Heinisch J.J.
      J. Bacteriol. 179:2987-2993(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKPYK2_YEAST
    AccessioniPrimary (citable) accession number: P52489
    Secondary accession number(s): D6W342
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2130 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3