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Protein

Pyruvate kinase 2

Gene

PYK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be used by cells under conditions in which the level of glycolytic flux is very low.

Miscellaneous

Present with 2130 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Enzyme regulationi

Not activated by fructose-1,6-bisphosphate.

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (TDH1), Glyceraldehyde-3-phosphate dehydrogenase 2 (TDH2), Glyceraldehyde-3-phosphate dehydrogenase 3 (TDH3)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase 2 (GPM2), Phosphoglycerate mutase 3 (GPM3), Phosphoglycerate mutase 1 (GPM1)
  4. Enolase 2 (ENO2), Enolase-related protein 2 (ERR2), Enolase-related protein 3 (ERR3), Enolase-related protein 1 (ERR1), Enolase 1 (ENO1)
  5. Pyruvate kinase 2 (PYK2), Pyruvate kinase 1 (CDC19)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51SubstrateBy similarity1
Metal bindingi53PotassiumBy similarity1
Metal bindingi55PotassiumBy similarity1
Metal bindingi86PotassiumBy similarity1
Metal bindingi87Potassium; via carbonyl oxygenBy similarity1
Sitei242Transition state stabilizerBy similarity1
Metal bindingi244MagnesiumSequence analysis1
Binding sitei267Substrate; via amide nitrogenBy similarity1
Metal bindingi268MagnesiumBy similarity1
Binding sitei268Substrate; via amide nitrogenBy similarity1
Binding sitei300SubstrateBy similarity1
Binding sitei339ADPSequence analysis1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • potassium ion binding Source: InterPro
  • pyruvate kinase activity Source: SGD

GO - Biological processi

  • pyruvate metabolic process Source: SGD

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciYEAST:YOR347C-MONOMER
ReactomeiR-SCE-6798695 Neutrophil degranulation
R-SCE-70171 Glycolysis
SABIO-RKP52489
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase 2 (EC:2.7.1.40)
Short name:
PK 2
Gene namesi
Name:PYK2
Ordered Locus Names:YOR347C
ORF Names:O6342
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR347C
SGDiS000005874 PYK2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121221 – 506Pyruvate kinase 2Add BLAST506

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP52489
PaxDbiP52489
PRIDEiP52489

PTM databases

iPTMnetiP52489

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi34732, 83 interactors
IntActiP52489, 12 interactors
MINTiP52489
STRINGi4932.YOR347C

Structurei

3D structure databases

ProteinModelPortaliP52489
SMRiP52489
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000008859
HOGENOMiHOG000021559
InParanoidiP52489
KOiK00873
OMAiIEHMCLL
OrthoDBiEOG092C1RXZ

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 2 hits
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P52489-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPESRLQRLA NLKIGTPQQL RRTSIIGTIG PKTNSCEAIT ALRKAGLNII
60 70 80 90 100
RLNFSHGSYE FHQSVIENAV KSEQQFPGRP LAIALDTKGP EIRTGRTLND
110 120 130 140 150
QDLYIPVDHQ MIFTTDASFA NTSNDKIMYI DYANLTKVIV PGRFIYVDDG
160 170 180 190 200
ILSFKVLQII DESNLRVQAV NSGYIASHKG VNLPNTDVDL PPLSAKDMKD
210 220 230 240 250
LQFGVRNGIH IVFASFIRTS EDVLSIRKAL GSEGQDIKII SKIENQQGLD
260 270 280 290 300
NFDEILEVTD GVMIARGDLG IEILAPEVLA IQKKLIAKCN LAGKPVICAT
310 320 330 340 350
QMLDSMTHNP RPTRAEVSDV GNAVLDGADC VMLSGETAKG DYPVNAVNIM
360 370 380 390 400
AATALIAEST IAHLALYDDL RDATPKPTST TETVAAAATA AILEQDGKAI
410 420 430 440 450
VVLSTTGNTA RLLSKYRPSC PIILVTRHAR TARIAHLYRG VFPFLYEPKR
460 470 480 490 500
LDDWGEDVHR RLKFGVEMAR SFGMVDNGDT VVSIQGFKGG VGHSNTLRIS

TVGQEF
Length:506
Mass (Da):55,195
Last modified:October 1, 1996 - v1
Checksum:iE0B36C298FA2E5E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95720 Genomic DNA Translation: CAA65034.1
Z75255 Genomic DNA Translation: CAA99675.1
BK006948 Genomic DNA Translation: DAA11108.1
PIRiS67256
RefSeqiNP_014992.3, NM_001183767.3

Genome annotation databases

EnsemblFungiiYOR347C; YOR347C; YOR347C
GeneIDi854529
KEGGisce:YOR347C

Similar proteinsi

Entry informationi

Entry nameiKPYK2_YEAST
AccessioniPrimary (citable) accession number: P52489
Secondary accession number(s): D6W342
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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