P52489 (KPYK2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase 2
Short name=PK 2
EC=2.7.1.40

Gene names

Name:	PYK2
Ordered Locus Names:	YOR347C
ORF Names:	O6342

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be used by cells under conditions in which the level of glycolytic flux is very low.
Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Enzyme regulation	Not activated by fructose-1,6-bisphosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Miscellaneous	Present with 2130 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway pyruvate metabolic process Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay PubMed 16962558. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Pyruvate kinase 2		PRO_0000112122
Sites
	Metal binding	53	1	Potassium By similarity
	Metal binding	55	1	Potassium By similarity
	Metal binding	86	1	Potassium By similarity
	Metal binding	87	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	244	1	Magnesium Potential
	Metal binding	268	1	Magnesium By similarity
	Binding site	51	1	Substrate By similarity
	Binding site	267	1	Substrate; via amide nitrogen By similarity
	Binding site	268	1	Substrate; via amide nitrogen By similarity
	Binding site	300	1	Substrate By similarity
	Binding site	339	1	ADP Potential
	Site	242	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	24	1	Phosphoserine Potential

Sequences

	Sequence		Length	Mass (Da)
	P52489 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E0B36C298FA2E5E8	FASTA	506	55,195
	10 20 30 40 50 60 MPESRLQRLA NLKIGTPQQL RRTSIIGTIG PKTNSCEAIT ALRKAGLNII RLNFSHGSYE 70 80 90 100 110 120 FHQSVIENAV KSEQQFPGRP LAIALDTKGP EIRTGRTLND QDLYIPVDHQ MIFTTDASFA 130 140 150 160 170 180 NTSNDKIMYI DYANLTKVIV PGRFIYVDDG ILSFKVLQII DESNLRVQAV NSGYIASHKG 190 200 210 220 230 240 VNLPNTDVDL PPLSAKDMKD LQFGVRNGIH IVFASFIRTS EDVLSIRKAL GSEGQDIKII 250 260 270 280 290 300 SKIENQQGLD NFDEILEVTD GVMIARGDLG IEILAPEVLA IQKKLIAKCN LAGKPVICAT 310 320 330 340 350 360 QMLDSMTHNP RPTRAEVSDV GNAVLDGADC VMLSGETAKG DYPVNAVNIM AATALIAEST 370 380 390 400 410 420 IAHLALYDDL RDATPKPTST TETVAAAATA AILEQDGKAI VVLSTTGNTA RLLSKYRPSC 430 440 450 460 470 480 PIILVTRHAR TARIAHLYRG VFPFLYEPKR LDDWGEDVHR RLKFGVEMAR SFGMVDNGDT 490 500 VVSIQGFKGG VGHSNTLRIS TVGQEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence analysis of a 40 kb segment on the right arm of yeast chromosome XV reveals 18 open reading frames including a new pyruvate kinase and three homologues to chromosome I genes." Purnelle B., Goffeau A. Yeast 12:1475-1481(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 90843 / S288c / FY73.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 90843 / S288c / FY73.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate." Boles E., Schulte F., Miosga T., Freidel K., Schlueter E., Zimmermann F.K., Hollenberg C.P., Heinisch J.J. J. Bacteriol. 179:2987-2993(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95720 Genomic DNA. Translation: CAA65034.1. Z75255 Genomic DNA. Translation: CAA99675.1. BK006948 Genomic DNA. Translation: DAA11108.1.
PIR	S67256.
RefSeq	NP_014992.3. NM_001183767.3.
3D structure databases
ProteinModelPortal	P52489.
SMR	P52489. Positions 4-502.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52489. 12 interactions.
MINT	MINT-2783027.
STRING	4932.YOR347C.
Proteomic databases
PaxDb	P52489.
PeptideAtlas	P52489.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR347C; YOR347C; YOR347C.
GeneID	854529.
KEGG	sce:YOR347C. sce:YOR349W.
Organism-specific databases
CYGD	YOR347c.
SGD	S000005874. PYK2.
Phylogenomic databases
eggNOG	COG0469.
GeneTree	ENSGT00390000008859.
HOGENOM	HOG000021559.
KO	K00873.
OMA	SHEDHRA.
OrthoDB	EOG43XZC1.
Enzyme and pathway databases
SABIO-RK	P52489.
UniPathway	UPA00109; UER00188.
Gene expression databases
Genevestigator	P52489.
GermOnline	YOR347C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976910.

Entry information

Entry name

KPYK2_YEAST

Accession

Primary (citable) accession number: P52489
Secondary accession number(s): D6W342

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program