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P52489 (KPYK2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase 2

Short name=PK 2
EC=2.7.1.40
Gene names
Name:PYK2
Ordered Locus Names:YOR347C
ORF Names:O6342
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be used by cells under conditions in which the level of glycolytic flux is very low.

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Enzyme regulation

Not activated by fructose-1,6-bisphosphate.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Miscellaneous

Present with 2130 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 506506Pyruvate kinase 2
PRO_0000112122

Sites

Metal binding531Potassium By similarity
Metal binding551Potassium By similarity
Metal binding861Potassium By similarity
Metal binding871Potassium; via carbonyl oxygen By similarity
Metal binding2441Magnesium Potential
Metal binding2681Magnesium By similarity
Binding site511Substrate By similarity
Binding site2671Substrate; via amide nitrogen By similarity
Binding site2681Substrate; via amide nitrogen By similarity
Binding site3001Substrate By similarity
Binding site3391ADP Potential
Site2421Transition state stabilizer By similarity

Amino acid modifications

Modified residue241Phosphoserine Potential

Sequences

Sequence LengthMass (Da)Tools
P52489 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: E0B36C298FA2E5E8

FASTA50655,195
        10         20         30         40         50         60 
MPESRLQRLA NLKIGTPQQL RRTSIIGTIG PKTNSCEAIT ALRKAGLNII RLNFSHGSYE 

        70         80         90        100        110        120 
FHQSVIENAV KSEQQFPGRP LAIALDTKGP EIRTGRTLND QDLYIPVDHQ MIFTTDASFA 

       130        140        150        160        170        180 
NTSNDKIMYI DYANLTKVIV PGRFIYVDDG ILSFKVLQII DESNLRVQAV NSGYIASHKG 

       190        200        210        220        230        240 
VNLPNTDVDL PPLSAKDMKD LQFGVRNGIH IVFASFIRTS EDVLSIRKAL GSEGQDIKII 

       250        260        270        280        290        300 
SKIENQQGLD NFDEILEVTD GVMIARGDLG IEILAPEVLA IQKKLIAKCN LAGKPVICAT 

       310        320        330        340        350        360 
QMLDSMTHNP RPTRAEVSDV GNAVLDGADC VMLSGETAKG DYPVNAVNIM AATALIAEST 

       370        380        390        400        410        420 
IAHLALYDDL RDATPKPTST TETVAAAATA AILEQDGKAI VVLSTTGNTA RLLSKYRPSC 

       430        440        450        460        470        480 
PIILVTRHAR TARIAHLYRG VFPFLYEPKR LDDWGEDVHR RLKFGVEMAR SFGMVDNGDT 

       490        500 
VVSIQGFKGG VGHSNTLRIS TVGQEF 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence analysis of a 40 kb segment on the right arm of yeast chromosome XV reveals 18 open reading frames including a new pyruvate kinase and three homologues to chromosome I genes."
Purnelle B., Goffeau A.
Yeast 12:1475-1481(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 90843 / S288c / FY73.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate."
Boles E., Schulte F., Miosga T., Freidel K., Schlueter E., Zimmermann F.K., Hollenberg C.P., Heinisch J.J.
J. Bacteriol. 179:2987-2993(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X95720 Genomic DNA. Translation: CAA65034.1.
Z75255 Genomic DNA. Translation: CAA99675.1.
BK006948 Genomic DNA. Translation: DAA11108.1.
PIRS67256.
RefSeqNP_014992.3. NM_001183767.3.

3D structure databases

ProteinModelPortalP52489.
SMRP52489. Positions 4-502.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34732. 24 interactions.
IntActP52489. 12 interactions.
MINTMINT-2783027.
STRING4932.YOR347C.

Proteomic databases

MaxQBP52489.
PaxDbP52489.
PeptideAtlasP52489.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR347C; YOR347C; YOR347C.
GeneID854529.
KEGGsce:YOR347C.

Organism-specific databases

CYGDYOR347c.
SGDS000005874. PYK2.

Phylogenomic databases

eggNOGCOG0469.
GeneTreeENSGT00390000008859.
HOGENOMHOG000021559.
KOK00873.
OMAPWILAIS.
OrthoDBEOG7M6DJC.

Enzyme and pathway databases

BioCycYEAST:YOR347C-MONOMER.
SABIO-RKP52489.
UniPathwayUPA00109; UER00188.

Gene expression databases

GenevestigatorP52489.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976910.

Entry information

Entry nameKPYK2_YEAST
AccessionPrimary (citable) accession number: P52489
Secondary accession number(s): D6W342
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways