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P52488 (UBA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-activating enzyme E1-like
Alternative name(s):
Polymerase-interacting protein 2
SMT3-activating enzyme subunit 2
Gene names
Name:UBA2
Synonyms:PIP2, UAL1
Ordered Locus Names:YDR390C
ORF Names:D9509.10
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length636 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The dimeric enzyme acts as a SMT3 E1 ligase. It mediates ATP-dependent activation of SMT3 and formation of a thioester with a conserved cysteine residue on AOS1. Ref.6

Pathway

Protein modification; protein sumoylation.

Subunit structure

Heterodimer of UBA2 and AOS1. The complex binds SMT3.

Subcellular location

Nucleus.

Post-translational modification

Multiubiquitinated in vivo.

Miscellaneous

Present with 18800 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ubiquitin-activating E1 family.

Sequence caution

The sequence CAA82980.1 differs from that shown. Reason: Frameshift at positions 180 and 268.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 636636Ubiquitin-activating enzyme E1-like
PRO_0000194979

Regions

Nucleotide binding28 – 336ATP By similarity
Nucleotide binding60 – 634ATP By similarity
Nucleotide binding121 – 1266ATP By similarity
Motif619 – 6224Nuclear localization signal Potential

Sites

Active site1771Glycyl thioester intermediate By similarity
Metal binding1621Zinc By similarity
Metal binding1651Zinc By similarity
Metal binding4351Zinc By similarity
Metal binding4381Zinc By similarity
Binding site521ATP By similarity
Binding site761ATP By similarity

Experimental info

Mutagenesis1771C → A or S: Loss of function.

Secondary structure

......................... 636
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P52488 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: DBF800E1458B3B10

FASTA63671,259
        10         20         30         40         50         60 
MPRETSLVTI IGEDSYKKLR SSRCLLVGAG GIGSELLKDI ILMEFGEIHI VDLDTIDLSN 

        70         80         90        100        110        120 
LNRQFLFRQK DIKQPKSTTA VKAVQHFNNS KLVPYQGNVM DISTFPLHWF EQFDIIFNAL 

       130        140        150        160        170        180 
DNLAARRYVN KISQFLSLPL IESGTAGFDG YMQPIIPGKT ECFECTKKET PKTFPVCTIR 

       190        200        210        220        230        240 
STPSQPIHCI VWAKNFLFNQ LFASETSGNE DDNNQDWGTD DAEEIKRIKQ ETNELYELQK 

       250        260        270        280        290        300 
IIISRDASRI PEILNKLFIQ DINKLLAIEN LWKTRTKPVP LSDSQINTPT KTAQSASNSV 

       310        320        330        340        350        360 
GTIQEQISNF INITQKLMDR YPKEQNHIEF DKDDADTLEF VATAANIRSH IFNIPMKSVF 

       370        380        390        400        410        420 
DIKQIAGNII PAIATTNAIV AGASSLISLR VLNLLKYAPT TKYTDLNMAF TAKASNLSQN 

       430        440        450        460        470        480 
RYLSNPKLAP PNKNCPVCSK VCRGVIKLSS DCLNKMKLSD FVVLIREKYS YPQDISLLDA 

       490        500        510        520        530        540 
SNQRLLFDYD FEDLNDRTLS EINLGNGSII LFSDEEGDTM IRKAIELFLD VDDELPCNTC 

       550        560        570        580        590        600 
SLPDVEVPLI KANNSPSKNE EEEKNEKGAD VVATTNSHGK DGIVILDDDE GEITIDAEPI 

       610        620        630 
NGSKKRPVDT EISEAPSNKR TKLVNEPTNS DIVELD 

« Hide

References

« Hide 'large scale' references
[1]"An essential yeast gene encoding a homolog of ubiquitin-activating enzyme."
Dohmen R.J., Stappen R., McGrath J.P., Forrova H., Kolarov J., Goffeau A., Varshavsky A.
J. Biol. Chem. 270:18099-18109(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Kolarov J.
Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]del Olmo M., Gross S., Moore C.L.
Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 200060 / W303.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer."
Johnson E.S., Schwienhorst I., Dohmen R.J., Blobel G.
EMBO J. 16:5509-5519(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z48725 Genomic DNA. Translation: CAA88617.1.
Z30326 Genomic DNA. Translation: CAA82980.1. Frameshift.
U17263 Genomic DNA. Translation: AAB46626.1.
U32274 Genomic DNA. Translation: AAB64832.1.
BK006938 Genomic DNA. Translation: DAA12234.1.
PIRA57178.
RefSeqNP_010678.3. NM_001180698.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ONGX-ray2.30A/C439-563[»]
3ONHX-ray1.60A439-563[»]
ProteinModelPortalP52488.
SMRP52488. Positions 19-552.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32451. 26 interactions.
DIPDIP-2296N.
IntActP52488. 9 interactions.
MINTMINT-657883.
STRING4932.YDR390C.

Proteomic databases

PaxDbP52488.
PeptideAtlasP52488.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR390C; YDR390C; YDR390C.
GeneID851998.
KEGGsce:YDR390C.

Organism-specific databases

CYGDYDR390c.
SGDS000002798. UBA2.

Phylogenomic databases

eggNOGCOG0476.
GeneTreeENSGT00550000074924.
HOGENOMHOG000216514.
KOK10685.
OMAPHHGNIM.
OrthoDBEOG7FZ07P.

Enzyme and pathway databases

BioCycYEAST:G3O-29938-MONOMER.
UniPathwayUPA00886.

Gene expression databases

GenevestigatorP52488.

Family and domain databases

Gene3D1.10.3240.10. 1 hit.
3.40.50.720. 2 hits.
InterProIPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR023280. Ub-like_act_enz_cat_cys_dom.
IPR000127. UBact_repeat.
IPR019572. Ubiquitin-activating_enzyme.
IPR018074. UBQ-activ_enz_E1_AS.
[Graphical view]
PfamPF00899. ThiF. 1 hit.
PF10585. UBA_e1_thiolCys. 1 hit.
PF02134. UBACT. 1 hit.
[Graphical view]
SUPFAMSSF69572. SSF69572. 2 hits.
PROSITEPS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970178.
PROP52488.

Entry information

Entry nameUBA2_YEAST
AccessionPrimary (citable) accession number: P52488
Secondary accession number(s): D6VT24
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways