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P52486

- UBCD4_DROME

UniProt

P52486 - UBCD4_DROME

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Protein
Ubiquitin-conjugating enzyme E2-22 kDa
Gene
UbcD4, CG8284
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid-amino acid ligase activity Source: InterPro
  3. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. protein ubiquitination Source: UniProtKB-UniPathway
  2. regulation of cell cycle Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiP52486.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-22 kDa (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:UbcD4
ORF Names:CG8284
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0015321. UbcD4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199Ubiquitin-conjugating enzyme E2-22 kDa
PRO_0000082522Add
BLAST

Proteomic databases

PaxDbiP52486.
PRIDEiP52486.

Expressioni

Tissue specificityi

During gastrulation, expression is highest in the invaginating posterior midgut primordium (PMG), high expression is also observed in the cephalic furrow and ventral ectodermal neurogenic region. In stage 10-11 embryos, expression is high in the pole cells present in the pocket formed by the PMG. During germ band retraction, expression appears to reinitiate in many tissues, especially the gut and nervous system. After dorsal closure, expression is detectable at low levels throughout the embryo.1 Publication

Developmental stagei

Expressed both maternally and zygotically. Embryonic expression is highest at 0-8 hours.1 Publication

Gene expression databases

BgeeiP52486.

Interactioni

Subunit structurei

Interacts with Rpn10.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Rpn10P550352EBI-224571,EBI-146479

Protein-protein interaction databases

BioGridi64522. 9 interactions.
DIPiDIP-20847N.
IntActiP52486. 3 interactions.
MINTiMINT-337672.

Structurei

3D structure databases

ProteinModelPortaliP52486.
SMRiP52486. Positions 1-199.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini161 – 19939UBA
Add
BLAST

Sequence similaritiesi

Contains 1 UBA domain.

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00670000098059.
InParanoidiP52486.
KOiK04649.
OMAiKHWTNAY.
OrthoDBiEOG7F513F.
PhylomeDBiP52486.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52486-1 [UniParc]FASTAAdd to Basket

« Hide

MANMAVSRIK REFKEVMRSE EIVQCSIKIE LVNDSWTELR GEIAGPPDTP    50
YEGGKFVLEI KVPETYPFNP PKVRFITRIW HPNISSVTGA ICLDILKDNW 100
AAAMTLRTVL LSLQALLAAA EPDDPQDAVV AYQFKDKYDL FLLTAKHWTN 150
AYAGGPHTFP DCDSKIQRLR DMGIDEHEAR AVLSKENWNL EKATEGLFS 199
Length:199
Mass (Da):22,510
Last modified:November 9, 2004 - v2
Checksum:i2875F9478632C630
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 363DSW → GQL in CAA63424. 1 Publication
Sequence conflicti73 – 731V → A in CAA63424. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X92838 mRNA. Translation: CAA63424.1.
Y11349 Genomic DNA. Translation: CAA72184.1.
AE014296 Genomic DNA. Translation: AAF50222.1.
AY060381 mRNA. Translation: AAL25420.1.
PIRiT08465.
RefSeqiNP_524010.2. NM_079286.3.
UniGeneiDm.7820.

Genome annotation databases

EnsemblMetazoaiFBtr0076395; FBpp0076124; FBgn0015321.
GeneIDi39133.
KEGGidme:Dmel_CG8284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X92838 mRNA. Translation: CAA63424.1 .
Y11349 Genomic DNA. Translation: CAA72184.1 .
AE014296 Genomic DNA. Translation: AAF50222.1 .
AY060381 mRNA. Translation: AAL25420.1 .
PIRi T08465.
RefSeqi NP_524010.2. NM_079286.3.
UniGenei Dm.7820.

3D structure databases

ProteinModelPortali P52486.
SMRi P52486. Positions 1-199.
ModBasei Search...

Protein-protein interaction databases

BioGridi 64522. 9 interactions.
DIPi DIP-20847N.
IntActi P52486. 3 interactions.
MINTi MINT-337672.

Proteomic databases

PaxDbi P52486.
PRIDEi P52486.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0076395 ; FBpp0076124 ; FBgn0015321 .
GeneIDi 39133.
KEGGi dme:Dmel_CG8284.

Organism-specific databases

CTDi 39133.
FlyBasei FBgn0015321. UbcD4.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00670000098059.
InParanoidi P52486.
KOi K04649.
OMAi KHWTNAY.
OrthoDBi EOG7F513F.
PhylomeDBi P52486.

Enzyme and pathway databases

UniPathwayi UPA00143 .
SignaLinki P52486.

Miscellaneous databases

GenomeRNAii 39133.
NextBioi 812082.
PROi P52486.

Gene expression databases

Bgeei P52486.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00627. UBA. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view ]
SMARTi SM00165. UBA. 1 hit.
[Graphical view ]
SUPFAMi SSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEi PS50030. UBA. 1 hit.
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "UbcD4, a ubiquitin-conjugating enzyme in Drosophila melanogaster expressed in pole cells."
    Canning M., Kirby R., Finnegan D.
    Mol. Genet. Genomics 266:907-913(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RPN10, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Canton-S and Oregon-R.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiUBCD4_DROME
AccessioniPrimary (citable) accession number: P52486
Secondary accession number(s): P91633
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 9, 2004
Last modified: September 3, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi