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P52486

- UBCD4_DROME

UniProt

P52486 - UBCD4_DROME

Protein

Ubiquitin-conjugating enzyme E2-22 kDa

Gene

UbcD4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (09 Nov 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins.1 PublicationPROSITE-ProRule annotation

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway
    2. regulation of cell cycle Source: FlyBase

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiP52486.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2-22 kDa (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein
    Ubiquitin-protein ligase
    Gene namesi
    Name:UbcD4
    ORF Names:CG8284
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0015321. UbcD4.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 199199Ubiquitin-conjugating enzyme E2-22 kDaPRO_0000082522Add
    BLAST

    Proteomic databases

    PaxDbiP52486.
    PRIDEiP52486.

    Expressioni

    Tissue specificityi

    During gastrulation, expression is highest in the invaginating posterior midgut primordium (PMG), high expression is also observed in the cephalic furrow and ventral ectodermal neurogenic region. In stage 10-11 embryos, expression is high in the pole cells present in the pocket formed by the PMG. During germ band retraction, expression appears to reinitiate in many tissues, especially the gut and nervous system. After dorsal closure, expression is detectable at low levels throughout the embryo.1 Publication

    Developmental stagei

    Expressed both maternally and zygotically. Embryonic expression is highest at 0-8 hours.1 Publication

    Gene expression databases

    BgeeiP52486.

    Interactioni

    Subunit structurei

    Interacts with Rpn10.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Rpn10P550352EBI-224571,EBI-146479

    Protein-protein interaction databases

    BioGridi64522. 9 interactions.
    DIPiDIP-20847N.
    IntActiP52486. 3 interactions.
    MINTiMINT-337672.

    Structurei

    3D structure databases

    ProteinModelPortaliP52486.
    SMRiP52486. Positions 1-199.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini161 – 19939UBAPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
    Contains 1 UBA domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00670000098059.
    InParanoidiP52486.
    KOiK04649.
    OMAiKHWTNAY.
    OrthoDBiEOG7F513F.
    PhylomeDBiP52486.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00627. UBA. 1 hit.
    PF00179. UQ_con. 1 hit.
    [Graphical view]
    SMARTiSM00165. UBA. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    SSF54495. SSF54495. 1 hit.
    PROSITEiPS50030. UBA. 1 hit.
    PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52486-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANMAVSRIK REFKEVMRSE EIVQCSIKIE LVNDSWTELR GEIAGPPDTP    50
    YEGGKFVLEI KVPETYPFNP PKVRFITRIW HPNISSVTGA ICLDILKDNW 100
    AAAMTLRTVL LSLQALLAAA EPDDPQDAVV AYQFKDKYDL FLLTAKHWTN 150
    AYAGGPHTFP DCDSKIQRLR DMGIDEHEAR AVLSKENWNL EKATEGLFS 199
    Length:199
    Mass (Da):22,510
    Last modified:November 9, 2004 - v2
    Checksum:i2875F9478632C630
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti34 – 363DSW → GQL in CAA63424. (PubMed:11862484)Curated
    Sequence conflicti73 – 731V → A in CAA63424. (PubMed:11862484)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92838 mRNA. Translation: CAA63424.1.
    Y11349 Genomic DNA. Translation: CAA72184.1.
    AE014296 Genomic DNA. Translation: AAF50222.1.
    AY060381 mRNA. Translation: AAL25420.1.
    PIRiT08465.
    RefSeqiNP_524010.2. NM_079286.3.
    UniGeneiDm.7820.

    Genome annotation databases

    EnsemblMetazoaiFBtr0076395; FBpp0076124; FBgn0015321.
    GeneIDi39133.
    KEGGidme:Dmel_CG8284.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92838 mRNA. Translation: CAA63424.1 .
    Y11349 Genomic DNA. Translation: CAA72184.1 .
    AE014296 Genomic DNA. Translation: AAF50222.1 .
    AY060381 mRNA. Translation: AAL25420.1 .
    PIRi T08465.
    RefSeqi NP_524010.2. NM_079286.3.
    UniGenei Dm.7820.

    3D structure databases

    ProteinModelPortali P52486.
    SMRi P52486. Positions 1-199.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 64522. 9 interactions.
    DIPi DIP-20847N.
    IntActi P52486. 3 interactions.
    MINTi MINT-337672.

    Proteomic databases

    PaxDbi P52486.
    PRIDEi P52486.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0076395 ; FBpp0076124 ; FBgn0015321 .
    GeneIDi 39133.
    KEGGi dme:Dmel_CG8284.

    Organism-specific databases

    CTDi 39133.
    FlyBasei FBgn0015321. UbcD4.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00670000098059.
    InParanoidi P52486.
    KOi K04649.
    OMAi KHWTNAY.
    OrthoDBi EOG7F513F.
    PhylomeDBi P52486.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    SignaLinki P52486.

    Miscellaneous databases

    GenomeRNAii 39133.
    NextBioi 812082.
    PROi P52486.

    Gene expression databases

    Bgeei P52486.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00627. UBA. 1 hit.
    PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SMARTi SM00165. UBA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    SSF54495. SSF54495. 1 hit.
    PROSITEi PS50030. UBA. 1 hit.
    PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "UbcD4, a ubiquitin-conjugating enzyme in Drosophila melanogaster expressed in pole cells."
      Canning M., Kirby R., Finnegan D.
      Mol. Genet. Genomics 266:907-913(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RPN10, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: Canton-S and Oregon-R.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.

    Entry informationi

    Entry nameiUBCD4_DROME
    AccessioniPrimary (citable) accession number: P52486
    Secondary accession number(s): P91633
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: November 9, 2004
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3