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Protein

Ubiquitin-conjugating enzyme E2-22 kDa

Gene

UbcD4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins.PROSITE-ProRule annotation1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. proteasome binding Source: FlyBase
  4. ubiquitin-like protein transferase activity Source: GO_Central
  5. ubiquitin protein ligase activity Source: GO_Central
  6. ubiquitin protein ligase binding Source: FlyBase
  7. ubiquitin-protein transferase activity Source: FlyBase

GO - Biological processi

  1. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  2. protein polyubiquitination Source: GO_Central
  3. regulation of cell cycle Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiP52486.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-22 kDa (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:UbcD4
ORF Names:CG8284
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0015321. UbcD4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. nucleus Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199Ubiquitin-conjugating enzyme E2-22 kDaPRO_0000082522Add
BLAST

Proteomic databases

PaxDbiP52486.
PRIDEiP52486.

Expressioni

Tissue specificityi

During gastrulation, expression is highest in the invaginating posterior midgut primordium (PMG), high expression is also observed in the cephalic furrow and ventral ectodermal neurogenic region. In stage 10-11 embryos, expression is high in the pole cells present in the pocket formed by the PMG. During germ band retraction, expression appears to reinitiate in many tissues, especially the gut and nervous system. After dorsal closure, expression is detectable at low levels throughout the embryo.1 Publication

Developmental stagei

Expressed both maternally and zygotically. Embryonic expression is highest at 0-8 hours.1 Publication

Gene expression databases

BgeeiP52486.

Interactioni

Subunit structurei

Interacts with Rpn10.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Rpn10P550352EBI-224571,EBI-146479

Protein-protein interaction databases

BioGridi64522. 9 interactions.
DIPiDIP-20847N.
IntActiP52486. 3 interactions.
MINTiMINT-337672.

Structurei

3D structure databases

ProteinModelPortaliP52486.
SMRiP52486. Positions 1-199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini161 – 19939UBAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
InParanoidiP52486.
KOiK04649.
OMAiHEMFILT.
OrthoDBiEOG7F513F.
PhylomeDBiP52486.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANMAVSRIK REFKEVMRSE EIVQCSIKIE LVNDSWTELR GEIAGPPDTP
60 70 80 90 100
YEGGKFVLEI KVPETYPFNP PKVRFITRIW HPNISSVTGA ICLDILKDNW
110 120 130 140 150
AAAMTLRTVL LSLQALLAAA EPDDPQDAVV AYQFKDKYDL FLLTAKHWTN
160 170 180 190
AYAGGPHTFP DCDSKIQRLR DMGIDEHEAR AVLSKENWNL EKATEGLFS
Length:199
Mass (Da):22,510
Last modified:November 9, 2004 - v2
Checksum:i2875F9478632C630
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 363DSW → GQL in CAA63424 (PubMed:11862484).Curated
Sequence conflicti73 – 731V → A in CAA63424 (PubMed:11862484).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92838 mRNA. Translation: CAA63424.1.
Y11349 Genomic DNA. Translation: CAA72184.1.
AE014296 Genomic DNA. Translation: AAF50222.1.
AY060381 mRNA. Translation: AAL25420.1.
PIRiT08465.
RefSeqiNP_001287013.1. NM_001300084.1.
NP_001287014.1. NM_001300085.1.
NP_524010.2. NM_079286.3.
UniGeneiDm.7820.

Genome annotation databases

EnsemblMetazoaiFBtr0076395; FBpp0076124; FBgn0015321.
FBtr0345603; FBpp0311672; FBgn0015321.
FBtr0345604; FBpp0311673; FBgn0015321.
GeneIDi39133.
KEGGidme:Dmel_CG8284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92838 mRNA. Translation: CAA63424.1.
Y11349 Genomic DNA. Translation: CAA72184.1.
AE014296 Genomic DNA. Translation: AAF50222.1.
AY060381 mRNA. Translation: AAL25420.1.
PIRiT08465.
RefSeqiNP_001287013.1. NM_001300084.1.
NP_001287014.1. NM_001300085.1.
NP_524010.2. NM_079286.3.
UniGeneiDm.7820.

3D structure databases

ProteinModelPortaliP52486.
SMRiP52486. Positions 1-199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64522. 9 interactions.
DIPiDIP-20847N.
IntActiP52486. 3 interactions.
MINTiMINT-337672.

Proteomic databases

PaxDbiP52486.
PRIDEiP52486.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0076395; FBpp0076124; FBgn0015321.
FBtr0345603; FBpp0311672; FBgn0015321.
FBtr0345604; FBpp0311673; FBgn0015321.
GeneIDi39133.
KEGGidme:Dmel_CG8284.

Organism-specific databases

CTDi39133.
FlyBaseiFBgn0015321. UbcD4.

Phylogenomic databases

eggNOGiCOG5078.
InParanoidiP52486.
KOiK04649.
OMAiHEMFILT.
OrthoDBiEOG7F513F.
PhylomeDBiP52486.

Enzyme and pathway databases

UniPathwayiUPA00143.
SignaLinkiP52486.

Miscellaneous databases

GenomeRNAii39133.
NextBioi812082.
PROiP52486.

Gene expression databases

BgeeiP52486.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "UbcD4, a ubiquitin-conjugating enzyme in Drosophila melanogaster expressed in pole cells."
    Canning M., Kirby R., Finnegan D.
    Mol. Genet. Genomics 266:907-913(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RPN10, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Canton-S and Oregon-R.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiUBCD4_DROME
AccessioniPrimary (citable) accession number: P52486
Secondary accession number(s): P91633
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 9, 2004
Last modified: April 1, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.